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The map label for this gene is gdhA [H]

Identifier: 108763589

GI number: 108763589

Start: 5325253

End: 5326782

Strand: Direct

Name: gdhA [H]

Synonym: MXAN_4327

Alternate gene names: 108763589

Gene position: 5325253-5326782 (Clockwise)

Preceding gene: 108757650

Following gene: 108761798

Centisome position: 58.26

GC content: 67.65

Gene sequence:

>1530_bases
ATGCGTGCCCCGGCCCCTACGCCCAAGCGCACCGTCTACACCGAGGCGATGGAGATCTTCCATCGTGCAGCGGACCTCAT
CGCGCTGGACAAGCGCGTCCGCCTCGAGCTGGAGGAGCCGGACTACGAGCACATCTTCTACGTCACGGCGAAGCTGAAGG
ACCGCCTCGTCCCGCTCTCCGAGGAGAAGGCGCGCACCTTCTCCAACCTTCCCGAGACGCAGGTGCGCAACAAGGAAGGC
CTGGAGCTGCTGGCCAACGGCAACATCATCCTCAACGGCCGCGCCCTGCTGGGCTCGGACGTGGACATCCGCCAGGGCCA
CCTGCGCCTGCCCGACGGCAAGGTGTACCAGTTGGTCCCCGGTGAGTCGCAGCGCTTCAAGGCCTACCGCGTGCAGCACA
ACCAGGCGCGCGGCCCCTACAAGGGCGGCCTGCGCTACCACCGCGAGGTGTCCCTGGACCTCTTCAAGGCCCTGGCCGCG
GAGATGACCTGGAAGACGGCCATCGCCGAGGTTCCGTTCGGCGGCGGCAAGGGCGGCATCCAGATCGACCCGCGCGAGTA
CGGCCGCGAGGAGATCGAGGCCATCACCCTGCGCTTCATGTACCGGCTCAAGAGCATGATTGGCCCGGACATCGACATCC
CGGCGCCGGACGTGGGCACCAACCCCGGCATCATGGCCCTGCTCTACCGCCAGTACTCGGACGGTGAGCGCGAGCGCCAC
AACCTGCGCGGCATCGTCACCGGCAAGGACGTGCGCATCGGCGGCTCCGAGGGCCGCCACAAGGCCACCGGCCAGGGCGT
CGCCTTCTGCATCGAGGACTACTACGCCGACCGCGGCGAGTCCGTGAAGGGCAAGACGTTCATCATCCAGGGCTTCGGCA
ACGTGGGCAGCCACGCCGCCAACATCCTGTCGCAGATGGGCGCCCGCCTGCTTGCGGTGAACGACGCCGACGGCACCATC
TTCAATGGTGACGGCATCGACGTGACCGCGCTCTCCGCCTACGTGCAGGACCCCAAGAACCTCAAGCGCAGCGTGCTCGG
CTTCCCGGGCGCCCAGCGCATCGAGAAGAAGGACCTCTGGGACGTCCAGGCGGACATCCTGGTCCCCGCGGCCCTGGGCG
GCGAAATCACCGCCGACGTGGCCGAGCGCCTGCGCGTCAAGCTGGTGGCCGAGGGCGCCAACGGCCCCACCACCCCGGAG
GCCGACCGCATCCTGGAGAAGCGCGGCATCGAGCTCATCCCGGACATCATCGCCAACGCCGGTGGTGTGACGGTGAGCTA
CTACGAGTGGATCCAGAACAAGCGCATGGAGCGCTGGAGCGAGGCCGAGGTCGACCAGCGGCTCGAGCGCGCCATGAAGC
GCAACTACCGCATCATCCGCGACATCTCGCGCAACCAGCCGCGCAAGACGGAGATGCACGACAGCCGCCAGTACTGCATC
GGTGAGACGGTGGACACCCGCTGCGCCGCCATGATTCTGGCGCTCAAGCGCATCGAGGCCCACTACCTCCTCGAGGGCTT
CTCGCAGTAG

Upstream 100 bases:

>100_bases
GCGCCTATGTACATAAGGGCCCCGCTACCCCCACGTGCCTCGCCCACGAGCGGCGACGCGACAAGGAGCATCTCCCGTCA
TGGCCAGCGAAGAGAATTTC

Downstream 100 bases:

>100_bases
TCGAAGCCACGGGCACCCGCCCACCGCTGGAAACACGAAGGGCACGGTCCTCCATCTGGAAGGCCGTGCCCTTTCGTTTT
CACCGGGTCCGGAGGTTGGC

Product: Glu/Leu/Phe/Val dehydrogenase family protein

Products: NA

Alternate protein names: GDH [H]

Number of amino acids: Translated: 509; Mature: 509

Protein sequence:

>509_residues
MRAPAPTPKRTVYTEAMEIFHRAADLIALDKRVRLELEEPDYEHIFYVTAKLKDRLVPLSEEKARTFSNLPETQVRNKEG
LELLANGNIILNGRALLGSDVDIRQGHLRLPDGKVYQLVPGESQRFKAYRVQHNQARGPYKGGLRYHREVSLDLFKALAA
EMTWKTAIAEVPFGGGKGGIQIDPREYGREEIEAITLRFMYRLKSMIGPDIDIPAPDVGTNPGIMALLYRQYSDGERERH
NLRGIVTGKDVRIGGSEGRHKATGQGVAFCIEDYYADRGESVKGKTFIIQGFGNVGSHAANILSQMGARLLAVNDADGTI
FNGDGIDVTALSAYVQDPKNLKRSVLGFPGAQRIEKKDLWDVQADILVPAALGGEITADVAERLRVKLVAEGANGPTTPE
ADRILEKRGIELIPDIIANAGGVTVSYYEWIQNKRMERWSEAEVDQRLERAMKRNYRIIRDISRNQPRKTEMHDSRQYCI
GETVDTRCAAMILALKRIEAHYLLEGFSQ

Sequences:

>Translated_509_residues
MRAPAPTPKRTVYTEAMEIFHRAADLIALDKRVRLELEEPDYEHIFYVTAKLKDRLVPLSEEKARTFSNLPETQVRNKEG
LELLANGNIILNGRALLGSDVDIRQGHLRLPDGKVYQLVPGESQRFKAYRVQHNQARGPYKGGLRYHREVSLDLFKALAA
EMTWKTAIAEVPFGGGKGGIQIDPREYGREEIEAITLRFMYRLKSMIGPDIDIPAPDVGTNPGIMALLYRQYSDGERERH
NLRGIVTGKDVRIGGSEGRHKATGQGVAFCIEDYYADRGESVKGKTFIIQGFGNVGSHAANILSQMGARLLAVNDADGTI
FNGDGIDVTALSAYVQDPKNLKRSVLGFPGAQRIEKKDLWDVQADILVPAALGGEITADVAERLRVKLVAEGANGPTTPE
ADRILEKRGIELIPDIIANAGGVTVSYYEWIQNKRMERWSEAEVDQRLERAMKRNYRIIRDISRNQPRKTEMHDSRQYCI
GETVDTRCAAMILALKRIEAHYLLEGFSQ
>Mature_509_residues
MRAPAPTPKRTVYTEAMEIFHRAADLIALDKRVRLELEEPDYEHIFYVTAKLKDRLVPLSEEKARTFSNLPETQVRNKEG
LELLANGNIILNGRALLGSDVDIRQGHLRLPDGKVYQLVPGESQRFKAYRVQHNQARGPYKGGLRYHREVSLDLFKALAA
EMTWKTAIAEVPFGGGKGGIQIDPREYGREEIEAITLRFMYRLKSMIGPDIDIPAPDVGTNPGIMALLYRQYSDGERERH
NLRGIVTGKDVRIGGSEGRHKATGQGVAFCIEDYYADRGESVKGKTFIIQGFGNVGSHAANILSQMGARLLAVNDADGTI
FNGDGIDVTALSAYVQDPKNLKRSVLGFPGAQRIEKKDLWDVQADILVPAALGGEITADVAERLRVKLVAEGANGPTTPE
ADRILEKRGIELIPDIIANAGGVTVSYYEWIQNKRMERWSEAEVDQRLERAMKRNYRIIRDISRNQPRKTEMHDSRQYCI
GETVDTRCAAMILALKRIEAHYLLEGFSQ

Specific function: Unknown

COG id: COG0334

COG function: function code E; Glutamate dehydrogenase/leucine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Glu/Leu/Phe/Val dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI31377775, Length=365, Percent_Identity=42.4657534246575, Blast_Score=272, Evalue=7e-73,
Organism=Homo sapiens, GI4885281, Length=364, Percent_Identity=42.8571428571429, Blast_Score=270, Evalue=3e-72,
Organism=Escherichia coli, GI1788059, Length=335, Percent_Identity=34.3283582089552, Blast_Score=185, Evalue=8e-48,
Organism=Caenorhabditis elegans, GI17544676, Length=327, Percent_Identity=41.8960244648318, Blast_Score=241, Evalue=9e-64,
Organism=Saccharomyces cerevisiae, GI6324951, Length=372, Percent_Identity=33.8709677419355, Blast_Score=163, Evalue=8e-41,
Organism=Saccharomyces cerevisiae, GI6319256, Length=365, Percent_Identity=34.2465753424658, Blast_Score=161, Evalue=2e-40,
Organism=Drosophila melanogaster, GI45553475, Length=361, Percent_Identity=40.1662049861496, Blast_Score=258, Evalue=8e-69,
Organism=Drosophila melanogaster, GI24649283, Length=324, Percent_Identity=43.8271604938272, Blast_Score=258, Evalue=8e-69,
Organism=Drosophila melanogaster, GI45549226, Length=360, Percent_Identity=40.2777777777778, Blast_Score=257, Evalue=1e-68,
Organism=Drosophila melanogaster, GI24649460, Length=318, Percent_Identity=39.937106918239, Blast_Score=224, Evalue=1e-58,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 9362 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006095
- InterPro:   IPR006096
- InterPro:   IPR006097
- InterPro:   IPR014362
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00208 ELFV_dehydrog; PF02812 ELFV_dehydrog_N [H]

EC number: =1.4.1.3 [H]

Molecular weight: Translated: 56971; Mature: 56971

Theoretical pI: Translated: 8.38; Mature: 8.38

Prosite motif: PS00074 GLFV_DEHYDROGENASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRAPAPTPKRTVYTEAMEIFHRAADLIALDKRVRLELEEPDYEHIFYVTAKLKDRLVPLS
CCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCEEEEEEEEHHHCCCCCC
EEKARTFSNLPETQVRNKEGLELLANGNIILNGRALLGSDVDIRQGHLRLPDGKVYQLVP
HHHHHHHHCCCHHHHCCCCCCEEEECCCEEEECCEEECCCCCCCCCEEECCCCCEEEECC
GESQRFKAYRVQHNQARGPYKGGLRYHREVSLDLFKALAAEMTWKTAIAEVPFGGGKGGI
CCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCE
QIDPREYGREEIEAITLRFMYRLKSMIGPDIDIPAPDVGTNPGIMALLYRQYSDGERERH
EECHHHHCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHCCCHHHHH
NLRGIVTGKDVRIGGSEGRHKATGQGVAFCIEDYYADRGESVKGKTFIIQGFGNVGSHAA
CCCEEEECCEEEECCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCEEEEECCCCCCHHHH
NILSQMGARLLAVNDADGTIFNGDGIDVTALSAYVQDPKNLKRSVLGFPGAQRIEKKDLW
HHHHHCCCEEEEEECCCCEEECCCCCCHHHHHHHHCCHHHHHHHHHCCCCHHHHCHHHHH
DVQADILVPAALGGEITADVAERLRVKLVAEGANGPTTPEADRILEKRGIELIPDIIANA
CCHHHEEEEECCCCCHHHHHHHHHEEEEEEECCCCCCCCHHHHHHHHCCCHHHHHHHCCC
GGVTVSYYEWIQNKRMERWSEAEVDQRLERAMKRNYRIIRDISRNQPRKTEMHDSRQYCI
CCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCHHCCCCCHHC
GETVDTRCAAMILALKRIEAHYLLEGFSQ
CCCHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MRAPAPTPKRTVYTEAMEIFHRAADLIALDKRVRLELEEPDYEHIFYVTAKLKDRLVPLS
CCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCEEEEEEEEHHHCCCCCC
EEKARTFSNLPETQVRNKEGLELLANGNIILNGRALLGSDVDIRQGHLRLPDGKVYQLVP
HHHHHHHHCCCHHHHCCCCCCEEEECCCEEEECCEEECCCCCCCCCEEECCCCCEEEECC
GESQRFKAYRVQHNQARGPYKGGLRYHREVSLDLFKALAAEMTWKTAIAEVPFGGGKGGI
CCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCE
QIDPREYGREEIEAITLRFMYRLKSMIGPDIDIPAPDVGTNPGIMALLYRQYSDGERERH
EECHHHHCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHCCCHHHHH
NLRGIVTGKDVRIGGSEGRHKATGQGVAFCIEDYYADRGESVKGKTFIIQGFGNVGSHAA
CCCEEEECCEEEECCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCEEEEECCCCCCHHHH
NILSQMGARLLAVNDADGTIFNGDGIDVTALSAYVQDPKNLKRSVLGFPGAQRIEKKDLW
HHHHHCCCEEEEEECCCCEEECCCCCCHHHHHHHHCCHHHHHHHHHCCCCHHHHCHHHHH
DVQADILVPAALGGEITADVAERLRVKLVAEGANGPTTPEADRILEKRGIELIPDIIANA
CCHHHEEEEECCCCCHHHHHHHHHEEEEEEECCCCCCCCHHHHHHHHCCCHHHHHHHCCC
GGVTVSYYEWIQNKRMERWSEAEVDQRLERAMKRNYRIIRDISRNQPRKTEMHDSRQYCI
CCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCHHCCCCCHHC
GETVDTRCAAMILALKRIEAHYLLEGFSQ
CCCHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9680336; 10360571; 9135121; 9654452; 10366510 [H]