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The map label for this gene is pnpA

Identifier: 108763580

GI number: 108763580

Start: 2404779

End: 2406947

Strand: Direct

Name: pnpA

Synonym: MXAN_2073

Alternate gene names: 108763580

Gene position: 2404779-2406947 (Clockwise)

Preceding gene: 108762422

Following gene: 108759305

Centisome position: 26.31

GC content: 67.04

Gene sequence:

>2169_bases
ATGTTGAAGAAGAGCGTCAAGATTGGCGAGAGCGAGCTGAGCATCGAAGTGGGCCGTCTGGCGAAGCAGGCCGACGGTTC
CGTGGTGGTCCGCTATGGCGACACCATGCTGCTGGTGACGGCGGTGAGCGCCCGGGAGAAGAAGGACATCGACTTCCTTC
CCCTGACGGTGGAGTACCAGGAGAAGCTGTATTCGGCCGGCCGCATCCCCGGCAGCTACTTCAAGCGCGAGGGCCGTCTC
ACGGAGAAGGAGACGCTGGCCAGCCGCCTGGTCGACCGCTCCTGCCGCCCGCTGTTCCCGGAAGGCTACGCGTACGAGAC
GCAGATCATCGCCAGCGTCATCTCCTCCGACCCGGAGAACGAGGGTGACATCCACGGCATCACCGGCGCCTCCGCGGCGC
TGTGGGTGTCGGACATCCCGTTTGACGGCCCCATTGCCGGCATCCGCGTGGGCCGCGTCGGCGGTCAGCTGGTGGCCAAC
CCCACCGCGAAGCAGCGCGAGCAGAGCGACCTGGACCTGGTCATGGCGGTGAGCCGTAAGGCCATCGTCATGGTGGAAGG
TGGCGCGGAGGAGGTCTCCGAGGCCGACATGGTCGCGGCGCTCGACTTCGGCTTCACGACGGCGCAGCCCGCGCTGGACC
TGCAGGACGAGCTGCGGCGCGAGCTGAACAAGCAGGTTCGCTCCTTCGAGAAGCCCGCCGCCGTGGACGAGGGCCTGCGC
GCCAAGGTGCGTGAGCTGGCCATGGACGGCATCAAGGCGGGCTACGGCATCAAGGAGAAGGGCGCGCGTTACGAGGCGCT
CGGCAAGACGAAGAAGGAGGCGCTCGCCAAGCTCAAGGAGCAGCTGGGCGACGGCTACACCCCCCTGGTGGAGAAGCACG
CCAAGGCGGTGGTGGAGGACCTGAAGTACGAGCACATGCGCGAGATGACGGTCAACGGTGGCCGCATCGGCGACCGTGGC
CACGACGTGGTCCGTTCGATTACGTGCGAGGTGGGCGTGCTCCCGCGCACCCACGGCAGCGCCGTCTTCACGCGCGGCGA
GACGCAGGCGCTCGTGGTCACCACGCTGGGTACCAGCGATGACGAGCAGCGCCTGGAGATGCTGGGCGGCATGGCCTTCA
AGCGCTTCATGCTGCACTACAACTTCCCGCCGTTCAGCGTGAACGAGACGAAGCCGCTGCGTGGCCCGGGCCGCCGTGAA
GTCGGCCACGGGGCGCTGGCGGAGCGCGCGCTGCGCAACATGGTGCCGAAGAGCGAGTCCTTCCCGTACACGGTGCGCTT
GGTGTCGGACATCCTGGAGTCCAACGGCTCCTCGTCCATGGCCTCCGTCTGCGGCGGCACGCTGGCGCTGATGGACGCGG
GTGTTCCGCTCAAGGCCCCTGTGGCGGGTATCGCCATGGGTCTGGTGAAGGAGGGCGACAAGATTGCCATCCTCTCCGAC
ATCCTCGGTGACGAGGACCACCTGGGCGACATGGACTTCAAGGTGTGCGGCACCTCGAAGGGCATCACGTCCATCCAGAT
GGACATCAAGATCACCGGCCTCACGACGGAAATCATGAGCCGCGCGCTGGAGCAGGCGCGTCAGGGCCGCCTCCACATCC
TGGGTGAGATGCTCAAGACGCTGGCCGAGTCCCGCAAGGAGATCAGCCAGTACGCGCCGCGCATCACCACCATCCAGATT
CGTCCCGAGTTCATCAAGAACGTCATCGGGCCGGGCGGCAAGGTCATCAAGGACATCATCGCCCGCACGGGTGCCGCGAT
TAACATCGAGGACTCGGGCCGCGTGGACATCGCCAGCGCGAACGGCGAGGCCGTGAAGGCCGCCATCGCGATGATTCAGG
CGCTGACCCGCGAGGCGGAGATCGGGAAGATCTACACGGGTACGGTGCGCAAGATCGCCGAGTTCGGCGCCTTCGTGGAG
CTGTTCCCGGGCACCGACGGCCTCATCCACATCTCCGAGCTGTCCGACAAGCGCGTCAAGAGCGTCTCCGACGTGCTGAA
CGAGGGCGACGAGGTGTTGGTGAAGGTCGTCAGCATCGACAAGACGGGCAAGATTCGCCTGTCGCGCAAGGAGGCCATGG
CGGAGCGCGCCGCGCAGCAGGGCGCCGCCGCCGGTGAGGCCGCCGCGCAGCCGGCGCCCGCGCCCACGCAGCCTGACGCC
AAGGCCTGA

Upstream 100 bases:

>100_bases
CGCTCAAACACCCCGAGGGCCCTCCCGGGGTGCCGGGTCCATTTCTCGGACCGGGTGCGCGCGGTGGGGTCTTCGCCCTG
AAGGATCCAGAGGCACGGAC

Downstream 100 bases:

>100_bases
TTCGCGCCCGAAAGCCCGGGCTCCTTCCTTGAGAGCCCGGCGCTCATGACGCCCCCTTCCGCCACCGCGCGGGAGGGGGT
GTTTTCGTTTCGGGCCGGGG

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase

Number of amino acids: Translated: 722; Mature: 722

Protein sequence:

>722_residues
MLKKSVKIGESELSIEVGRLAKQADGSVVVRYGDTMLLVTAVSAREKKDIDFLPLTVEYQEKLYSAGRIPGSYFKREGRL
TEKETLASRLVDRSCRPLFPEGYAYETQIIASVISSDPENEGDIHGITGASAALWVSDIPFDGPIAGIRVGRVGGQLVAN
PTAKQREQSDLDLVMAVSRKAIVMVEGGAEEVSEADMVAALDFGFTTAQPALDLQDELRRELNKQVRSFEKPAAVDEGLR
AKVRELAMDGIKAGYGIKEKGARYEALGKTKKEALAKLKEQLGDGYTPLVEKHAKAVVEDLKYEHMREMTVNGGRIGDRG
HDVVRSITCEVGVLPRTHGSAVFTRGETQALVVTTLGTSDDEQRLEMLGGMAFKRFMLHYNFPPFSVNETKPLRGPGRRE
VGHGALAERALRNMVPKSESFPYTVRLVSDILESNGSSSMASVCGGTLALMDAGVPLKAPVAGIAMGLVKEGDKIAILSD
ILGDEDHLGDMDFKVCGTSKGITSIQMDIKITGLTTEIMSRALEQARQGRLHILGEMLKTLAESRKEISQYAPRITTIQI
RPEFIKNVIGPGGKVIKDIIARTGAAINIEDSGRVDIASANGEAVKAAIAMIQALTREAEIGKIYTGTVRKIAEFGAFVE
LFPGTDGLIHISELSDKRVKSVSDVLNEGDEVLVKVVSIDKTGKIRLSRKEAMAERAAQQGAAAGEAAAQPAPAPTQPDA
KA

Sequences:

>Translated_722_residues
MLKKSVKIGESELSIEVGRLAKQADGSVVVRYGDTMLLVTAVSAREKKDIDFLPLTVEYQEKLYSAGRIPGSYFKREGRL
TEKETLASRLVDRSCRPLFPEGYAYETQIIASVISSDPENEGDIHGITGASAALWVSDIPFDGPIAGIRVGRVGGQLVAN
PTAKQREQSDLDLVMAVSRKAIVMVEGGAEEVSEADMVAALDFGFTTAQPALDLQDELRRELNKQVRSFEKPAAVDEGLR
AKVRELAMDGIKAGYGIKEKGARYEALGKTKKEALAKLKEQLGDGYTPLVEKHAKAVVEDLKYEHMREMTVNGGRIGDRG
HDVVRSITCEVGVLPRTHGSAVFTRGETQALVVTTLGTSDDEQRLEMLGGMAFKRFMLHYNFPPFSVNETKPLRGPGRRE
VGHGALAERALRNMVPKSESFPYTVRLVSDILESNGSSSMASVCGGTLALMDAGVPLKAPVAGIAMGLVKEGDKIAILSD
ILGDEDHLGDMDFKVCGTSKGITSIQMDIKITGLTTEIMSRALEQARQGRLHILGEMLKTLAESRKEISQYAPRITTIQI
RPEFIKNVIGPGGKVIKDIIARTGAAINIEDSGRVDIASANGEAVKAAIAMIQALTREAEIGKIYTGTVRKIAEFGAFVE
LFPGTDGLIHISELSDKRVKSVSDVLNEGDEVLVKVVSIDKTGKIRLSRKEAMAERAAQQGAAAGEAAAQPAPAPTQPDA
KA
>Mature_722_residues
MLKKSVKIGESELSIEVGRLAKQADGSVVVRYGDTMLLVTAVSAREKKDIDFLPLTVEYQEKLYSAGRIPGSYFKREGRL
TEKETLASRLVDRSCRPLFPEGYAYETQIIASVISSDPENEGDIHGITGASAALWVSDIPFDGPIAGIRVGRVGGQLVAN
PTAKQREQSDLDLVMAVSRKAIVMVEGGAEEVSEADMVAALDFGFTTAQPALDLQDELRRELNKQVRSFEKPAAVDEGLR
AKVRELAMDGIKAGYGIKEKGARYEALGKTKKEALAKLKEQLGDGYTPLVEKHAKAVVEDLKYEHMREMTVNGGRIGDRG
HDVVRSITCEVGVLPRTHGSAVFTRGETQALVVTTLGTSDDEQRLEMLGGMAFKRFMLHYNFPPFSVNETKPLRGPGRRE
VGHGALAERALRNMVPKSESFPYTVRLVSDILESNGSSSMASVCGGTLALMDAGVPLKAPVAGIAMGLVKEGDKIAILSD
ILGDEDHLGDMDFKVCGTSKGITSIQMDIKITGLTTEIMSRALEQARQGRLHILGEMLKTLAESRKEISQYAPRITTIQI
RPEFIKNVIGPGGKVIKDIIARTGAAINIEDSGRVDIASANGEAVKAAIAMIQALTREAEIGKIYTGTVRKIAEFGAFVE
LFPGTDGLIHISELSDKRVKSVSDVLNEGDEVLVKVVSIDKTGKIRLSRKEAMAERAAQQGAAAGEAAAQPAPAPTQPDA
KA

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain

Homologues:

Organism=Homo sapiens, GI188528628, Length=713, Percent_Identity=38.148667601683, Blast_Score=460, Evalue=1e-129,
Organism=Escherichia coli, GI145693187, Length=691, Percent_Identity=50.6512301013025, Blast_Score=655, Evalue=0.0,
Organism=Caenorhabditis elegans, GI115534063, Length=720, Percent_Identity=35.2777777777778, Blast_Score=356, Evalue=3e-98,
Organism=Caenorhabditis elegans, GI17535281, Length=76, Percent_Identity=51.3157894736842, Blast_Score=68, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6320850, Length=90, Percent_Identity=38.8888888888889, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI281362905, Length=708, Percent_Identity=36.2994350282486, Blast_Score=430, Evalue=1e-120,
Organism=Drosophila melanogaster, GI24651641, Length=708, Percent_Identity=36.2994350282486, Blast_Score=430, Evalue=1e-120,
Organism=Drosophila melanogaster, GI24651643, Length=708, Percent_Identity=36.2994350282486, Blast_Score=430, Evalue=1e-120,
Organism=Drosophila melanogaster, GI161079377, Length=655, Percent_Identity=36.1832061068702, Blast_Score=398, Evalue=1e-111,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): PNP_MYXXD (Q1DAM1)

Other databases:

- EMBL:   CP000113
- RefSeq:   YP_630302.1
- ProteinModelPortal:   Q1DAM1
- STRING:   Q1DAM1
- GeneID:   4107339
- GenomeReviews:   CP000113_GR
- KEGG:   mxa:MXAN_2073
- TIGR:   MXAN_2073
- eggNOG:   COG1185
- HOGENOM:   HBG382411
- OMA:   YGETVVL
- PhylomeDB:   Q1DAM1
- ProtClustDB:   PRK11824
- BioCyc:   MXAN246197:MXAN_2073-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_01595
- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR009019
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967
- Gene3D:   G3DSA:2.40.50.140
- Gene3D:   G3DSA:1.10.10.400
- PANTHER:   PTHR11252
- PIRSF:   PIRSF005499
- SMART:   SM00322
- SMART:   SM00316
- TIGRFAMs:   TIGR03591

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1; SSF46915 3_ExoRNase; SSF55666 3_ExoRNase; SSF54814 KH_prok; SSF50249 Nucleic_acid_OB; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.7.8

Molecular weight: Translated: 77797; Mature: 77797

Theoretical pI: Translated: 6.54; Mature: 6.54

Prosite motif: PS50084 KH_TYPE_1; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKKSVKIGESELSIEVGRLAKQADGSVVVRYGDTMLLVTAVSAREKKDIDFLPLTVEYQ
CCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCEEEEEEEHHHCCCCCCCEEEEEHHHH
EKLYSAGRIPGSYFKREGRLTEKETLASRLVDRSCRPLFPEGYAYETQIIASVISSDPEN
HHHHHCCCCCHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCC
EGDIHGITGASAALWVSDIPFDGPIAGIRVGRVGGQLVANPTAKQREQSDLDLVMAVSRK
CCCCCEECCCCCEEEEECCCCCCCCCCEEEECCCCEEEECCCHHHHHHCCCHHHEEECCC
AIVMVEGGAEEVSEADMVAALDFGFTTAQPALDLQDELRRELNKQVRSFEKPAAVDEGLR
EEEEEECCHHHHHHHHHEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHH
AKVRELAMDGIKAGYGIKEKGARYEALGKTKKEALAKLKEQLGDGYTPLVEKHAKAVVED
HHHHHHHHHHHHCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
LKYEHMREMTVNGGRIGDRGHDVVRSITCEVGVLPRTHGSAVFTRGETQALVVTTLGTSD
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHHEECCCCCCCCEEEECCCCCEEEEEEECCCC
DEQRLEMLGGMAFKRFMLHYNFPPFSVNETKPLRGPGRREVGHGALAERALRNMVPKSES
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCC
FPYTVRLVSDILESNGSSSMASVCGGTLALMDAGVPLKAPVAGIAMGLVKEGDKIAILSD
CCCHHHHHHHHHHCCCCCHHHHHHCCHHEEHCCCCCCCCCHHHHHHHHHCCCCCEEEEHH
ILGDEDHLGDMDFKVCGTSKGITSIQMDIKITGLTTEIMSRALEQARQGRLHILGEMLKT
HCCCCCCCCCCCEEEECCCCCCEEEEEEEEEECCHHHHHHHHHHHHHCCHHHHHHHHHHH
LAESRKEISQYAPRITTIQIRPEFIKNVIGPGGKVIKDIIARTGAAINIEDSGRVDIASA
HHHHHHHHHHHCCCEEEEEECHHHHHHHHCCCHHHHHHHHHHCCCEEEECCCCCEEEECC
NGEAVKAAIAMIQALTREAEIGKIYTGTVRKIAEFGAFVELFPGTDGLIHISELSDKRVK
CCHHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHCCEEEEECCCCCEEEEHHHCHHHHH
SVSDVLNEGDEVLVKVVSIDKTGKIRLSRKEAMAERAAQQGAAAGEAAAQPAPAPTQPDA
HHHHHHCCCCEEEEEEEEECCCCCEEEHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
KA
CC
>Mature Secondary Structure
MLKKSVKIGESELSIEVGRLAKQADGSVVVRYGDTMLLVTAVSAREKKDIDFLPLTVEYQ
CCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCEEEEEEEHHHCCCCCCCEEEEEHHHH
EKLYSAGRIPGSYFKREGRLTEKETLASRLVDRSCRPLFPEGYAYETQIIASVISSDPEN
HHHHHCCCCCHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCC
EGDIHGITGASAALWVSDIPFDGPIAGIRVGRVGGQLVANPTAKQREQSDLDLVMAVSRK
CCCCCEECCCCCEEEEECCCCCCCCCCEEEECCCCEEEECCCHHHHHHCCCHHHEEECCC
AIVMVEGGAEEVSEADMVAALDFGFTTAQPALDLQDELRRELNKQVRSFEKPAAVDEGLR
EEEEEECCHHHHHHHHHEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHH
AKVRELAMDGIKAGYGIKEKGARYEALGKTKKEALAKLKEQLGDGYTPLVEKHAKAVVED
HHHHHHHHHHHHCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
LKYEHMREMTVNGGRIGDRGHDVVRSITCEVGVLPRTHGSAVFTRGETQALVVTTLGTSD
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHHEECCCCCCCCEEEECCCCCEEEEEEECCCC
DEQRLEMLGGMAFKRFMLHYNFPPFSVNETKPLRGPGRREVGHGALAERALRNMVPKSES
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCC
FPYTVRLVSDILESNGSSSMASVCGGTLALMDAGVPLKAPVAGIAMGLVKEGDKIAILSD
CCCHHHHHHHHHHCCCCCHHHHHHCCHHEEHCCCCCCCCCHHHHHHHHHCCCCCEEEEHH
ILGDEDHLGDMDFKVCGTSKGITSIQMDIKITGLTTEIMSRALEQARQGRLHILGEMLKT
HCCCCCCCCCCCEEEECCCCCCEEEEEEEEEECCHHHHHHHHHHHHHCCHHHHHHHHHHH
LAESRKEISQYAPRITTIQIRPEFIKNVIGPGGKVIKDIIARTGAAINIEDSGRVDIASA
HHHHHHHHHHHCCCEEEEEECHHHHHHHHCCCHHHHHHHHHHCCCEEEECCCCCEEEECC
NGEAVKAAIAMIQALTREAEIGKIYTGTVRKIAEFGAFVELFPGTDGLIHISELSDKRVK
CCHHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHCCEEEEECCCCCEEEEHHHCHHHHH
SVSDVLNEGDEVLVKVVSIDKTGKIRLSRKEAMAERAAQQGAAAGEAAAQPAPAPTQPDA
HHHHHHCCCCEEEEEEEEECCCCCEEEHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
KA
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA