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The map label for this gene is nifS1 [H]

Identifier: 108763530

GI number: 108763530

Start: 3495866

End: 3497011

Strand: Reverse

Name: nifS1 [H]

Synonym: MXAN_2983

Alternate gene names: 108763530

Gene position: 3497011-3495866 (Counterclockwise)

Preceding gene: 108757496

Following gene: 108760545

Centisome position: 38.26

GC content: 73.82

Gene sequence:

>1146_bases
GTGACGTACTGGGACTACAACGCGGCCGCGCCGATACGAGCGGAGGTCGCGACGCTGCTGTCGCGCGCCTTCGCGTCGAG
CGGCTACGGCAATGCGTCCAGCGTGCACCAGGAGGGCCGCGCGGCCCGGGCCCGGTTGGACGCGGCACGGGCGCAGGTGG
CCCGCGTGCTTGGCTGCGAGCCGAAGGAAGTCAGCTTCACCGGCTCCGGCAGCGAGGCGGACGCGCTGGCGCTCGTGGGC
GCCTGGCACATGCGGCCCCAGCCCGAGCGGCGGCGGGTGGTGACTTCCGCGGTGGAGCACCCCGCCCTGCTGGGCGCGGT
GGCGCAGTTGGAGCGGGAAGGCGCTTACGTCGTGCGGGTGGCGCCTGGTCCGGACGGGCGCGTGCGCGAAGCCGACATGC
TGGAGGCCCTGACGCCAGACGCGGCCTTGTGCTCGTTGATGTGGGCCAACAACGAGACGGGCGTGCTCCAGCCCGCGCGG
GAGGTGGCGCTCGCGTGCCGGCAGCGCGGCGTGCTGTTCCACACGGACGCGGTGCAGGCCGCGGGCAAGGTGCCCCTGTC
CCTGCGCGAGGTGGACGCGGACCTGTTGTCCCTGTCCGCGCACAAGTTCGGCGGCCCTCCGGGCGTGGGCGTGCTGATGG
TCCGCAAGAGCGTGGACGTCCGCGCGCTCACGCCGGGTCATCAAGAAGGCGGCCGTCGCGGAGGGACGCAGAACGTGCCC
TACGCGGAGGCGCTGGCGCTCGCGCTCGCGTTGGCCGATGCGGAGCTGACTGAGACAACGGCGCGGCTGACCACGCTCCG
CGATGCGTTCGAGCGGGAGGTGACGGCGCGACTGCCCGGCGTGCACATCAACGGCGGCGCGGCCCCGCGCGTGCCCAACA
CCAGCAACCTGCGCTTCGACGGCGTGGAGGGTGAGGCGCTGCTCATCGCCCTGGATCTGGAGGGCATCTGCGTGTCCTCC
GGCGCCGCCTGCGCGTCCGGCACGCTGACGCCGTCGCATGTCCTGCGGGCCATGGGCCTGTCGCCCGCGCAGGCCCGAGG
CAGCCTTCGCTTCAGCATGGGCCCCGGCACGACGGAAGCGGACGTGGCGCGCGTGGTGGACGCGCTCGTCCGACACGTGC
CGCATGTCCGCGCGCTGGCCGGCTAG

Upstream 100 bases:

>100_bases
CGCAGCGCAAGGCGCTCAAGCGCGAGCTGGTGGCCAAGTTCCTGGAGGCATTCGGCGTCGCCAACGAGCGGCCGGTGTCG
CTGCTGTACGCGCAGGACTC

Downstream 100 bases:

>100_bases
GACACGGAACGTCGGCTCAGGGGCAGCCGAAGATGGAAGGTCGCCCCGCCCACAGCACGGACTTCACCGACAACGAGCAG
CGCATGGGCCACCCAGGTGA

Product: putative cysteine desulfurase

Products: NA

Alternate protein names: Nitrogenase metalloclusters biosynthesis protein nifS1 [H]

Number of amino acids: Translated: 381; Mature: 380

Protein sequence:

>381_residues
MTYWDYNAAAPIRAEVATLLSRAFASSGYGNASSVHQEGRAARARLDAARAQVARVLGCEPKEVSFTGSGSEADALALVG
AWHMRPQPERRRVVTSAVEHPALLGAVAQLEREGAYVVRVAPGPDGRVREADMLEALTPDAALCSLMWANNETGVLQPAR
EVALACRQRGVLFHTDAVQAAGKVPLSLREVDADLLSLSAHKFGGPPGVGVLMVRKSVDVRALTPGHQEGGRRGGTQNVP
YAEALALALALADAELTETTARLTTLRDAFEREVTARLPGVHINGGAAPRVPNTSNLRFDGVEGEALLIALDLEGICVSS
GAACASGTLTPSHVLRAMGLSPAQARGSLRFSMGPGTTEADVARVVDALVRHVPHVRALAG

Sequences:

>Translated_381_residues
MTYWDYNAAAPIRAEVATLLSRAFASSGYGNASSVHQEGRAARARLDAARAQVARVLGCEPKEVSFTGSGSEADALALVG
AWHMRPQPERRRVVTSAVEHPALLGAVAQLEREGAYVVRVAPGPDGRVREADMLEALTPDAALCSLMWANNETGVLQPAR
EVALACRQRGVLFHTDAVQAAGKVPLSLREVDADLLSLSAHKFGGPPGVGVLMVRKSVDVRALTPGHQEGGRRGGTQNVP
YAEALALALALADAELTETTARLTTLRDAFEREVTARLPGVHINGGAAPRVPNTSNLRFDGVEGEALLIALDLEGICVSS
GAACASGTLTPSHVLRAMGLSPAQARGSLRFSMGPGTTEADVARVVDALVRHVPHVRALAG
>Mature_380_residues
TYWDYNAAAPIRAEVATLLSRAFASSGYGNASSVHQEGRAARARLDAARAQVARVLGCEPKEVSFTGSGSEADALALVGA
WHMRPQPERRRVVTSAVEHPALLGAVAQLEREGAYVVRVAPGPDGRVREADMLEALTPDAALCSLMWANNETGVLQPARE
VALACRQRGVLFHTDAVQAAGKVPLSLREVDADLLSLSAHKFGGPPGVGVLMVRKSVDVRALTPGHQEGGRRGGTQNVPY
AEALALALALADAELTETTARLTTLRDAFEREVTARLPGVHINGGAAPRVPNTSNLRFDGVEGEALLIALDLEGICVSSG
AACASGTLTPSHVLRAMGLSPAQARGSLRFSMGPGTTEADVARVVDALVRHVPHVRALAG

Specific function: Catalyzes the removal of elemental sulfur atoms from cysteine to produce alanine. Seems to participate in the biosynthesis of the nitrogenase metalloclusters by providing the inorganic sulfur required for the Fe-S core formation [H]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=382, Percent_Identity=35.8638743455497, Blast_Score=231, Evalue=7e-61,
Organism=Homo sapiens, GI156713448, Length=408, Percent_Identity=30.1470588235294, Blast_Score=161, Evalue=1e-39,
Organism=Escherichia coli, GI48994898, Length=383, Percent_Identity=36.8146214099217, Blast_Score=243, Evalue=2e-65,
Organism=Escherichia coli, GI1789175, Length=391, Percent_Identity=27.1099744245524, Blast_Score=88, Evalue=8e-19,
Organism=Caenorhabditis elegans, GI25143064, Length=382, Percent_Identity=36.1256544502618, Blast_Score=239, Evalue=2e-63,
Organism=Caenorhabditis elegans, GI17533177, Length=317, Percent_Identity=33.1230283911672, Blast_Score=152, Evalue=3e-37,
Organism=Saccharomyces cerevisiae, GI6319831, Length=384, Percent_Identity=35.4166666666667, Blast_Score=220, Evalue=2e-58,
Organism=Drosophila melanogaster, GI20129463, Length=383, Percent_Identity=36.8146214099217, Blast_Score=222, Evalue=3e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR017772
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 39895; Mature: 39764

Theoretical pI: Translated: 7.31; Mature: 7.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTYWDYNAAAPIRAEVATLLSRAFASSGYGNASSVHQEGRAARARLDAARAQVARVLGCE
CCEECCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PKEVSFTGSGSEADALALVGAWHMRPQPERRRVVTSAVEHPALLGAVAQLEREGAYVVRV
CCCEEEECCCCCCCCEEEEEHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
APGPDGRVREADMLEALTPDAALCSLMWANNETGVLQPAREVALACRQRGVLFHTDAVQA
CCCCCCCCCHHHHHHHHCCCHHHHHEEECCCCCCCCCHHHHHHHHHHHCCCEEECHHHHH
AGKVPLSLREVDADLLSLSAHKFGGPPGVGVLMVRKSVDVRALTPGHQEGGRRGGTQNVP
CCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCEEEECCCCHHCCCCCCCCCCC
YAEALALALALADAELTETTARLTTLRDAFEREVTARLPGVHINGGAAPRVPNTSNLRFD
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCEEEE
GVEGEALLIALDLEGICVSSGAACASGTLTPSHVLRAMGLSPAQARGSLRFSMGPGTTEA
CCCCCEEEEEEECCCEEECCCCCCCCCCCCHHHHHHHHCCCCHHHCCCEEEECCCCCCHH
DVARVVDALVRHVPHVRALAG
HHHHHHHHHHHHCCCHHHHCC
>Mature Secondary Structure 
TYWDYNAAAPIRAEVATLLSRAFASSGYGNASSVHQEGRAARARLDAARAQVARVLGCE
CEECCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PKEVSFTGSGSEADALALVGAWHMRPQPERRRVVTSAVEHPALLGAVAQLEREGAYVVRV
CCCEEEECCCCCCCCEEEEEHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
APGPDGRVREADMLEALTPDAALCSLMWANNETGVLQPAREVALACRQRGVLFHTDAVQA
CCCCCCCCCHHHHHHHHCCCHHHHHEEECCCCCCCCCHHHHHHHHHHHCCCEEECHHHHH
AGKVPLSLREVDADLLSLSAHKFGGPPGVGVLMVRKSVDVRALTPGHQEGGRRGGTQNVP
CCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCEEEECCCCHHCCCCCCCCCCC
YAEALALALALADAELTETTARLTTLRDAFEREVTARLPGVHINGGAAPRVPNTSNLRFD
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCEEEE
GVEGEALLIALDLEGICVSSGAACASGTLTPSHVLRAMGLSPAQARGSLRFSMGPGTTEA
CCCCCEEEEEEECCCEEECCCCCCCCCCCCHHHHHHHHCCCCHHHCCCEEEECCCCCCHH
DVARVVDALVRHVPHVRALAG
HHHHHHHHHHHHCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7568132 [H]