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The map label for this gene is iaaA [H]

Identifier: 108762857

GI number: 108762857

Start: 1350467

End: 1351522

Strand: Direct

Name: iaaA [H]

Synonym: MXAN_1160

Alternate gene names: 108762857

Gene position: 1350467-1351522 (Clockwise)

Preceding gene: 108757287

Following gene: 108758831

Centisome position: 14.78

GC content: 70.55

Gene sequence:

>1056_bases
GTGGGCTGTGCCACCCAGACTGGGGCTGCTCGCACCGACAGCGAGCAGCCCACCTCGCGTGATGCACCCGCGCCCAAGCC
GAAGTGGGGGCTGGTCATCCACGGCGGCGCGGGTGTCATCTCCCGGGAGAATCTCTCCCCAGAGCGCGAGGAGGAGGTCC
GCGCCGCGCTGACCCAGTCCCTCCAGGCGGGACACGCGGTGTTGGCCAGTGGTGGCTCCGCGCTGGACGCAGTGAGCGCC
GCGGTCCGCATCCTGGAGGACTCTCCGTACTTCAACGCGGGCAAGGGCGCCGTCTTCACGCACGACGGGGTCAACGAGCT
GGATGCCGCCATCATGGATGGCACGACGCGCAAGGCGGGGGCCGTGGCGGGCTTGCGGCACGTGAAGAACCCCATCTCGC
TGGCGCGGCTCGTCATGGAGCAGTCGCCGCACGTGATGATGGTGGGGGAGGGCGCGGAGGCCTTCGCGAAGTCGCAGGGT
GTGGAGTTGGTGGACCCGAAGTACTTCTACACGGAGGACCGGTGGCAGGGCCTCCAGCGCGCGCTGGAGAAGGAGCGCTC
GCAGCCGCAGCCATCCTCCGCGCTCCCGGCGGGGTATGACCCGGTGAGCGGGGACCACAAGTTCGGCACCGTGGGCGCCG
TGGCCCTGGACCAGACGGGCGCGCTCGCCGCGGCCACGTCCACGGGAGGCATGACGAACAAGCGCTACGGCCGCGTGGGG
GACTCGCCCATCATCGGCGCGGGGACGTATGCGGACGCGCGCTGCGCCGTGTCCGCCACCGGCCATGGCGAGTTCTTCAT
TCGCTACACCGTGGCGCGCGACATCTGCGCCCGCGTGGAATACCAGAACGTGCCGTTGCCGGAGGCCGCCGACGTCGTCA
TCAACGACGTGCTGGTGAAGGTCGGCGGCGAGGGCGGCGTCATCGCGATGGACCGCGAGGGCAACGTGGCCATGCCCTTC
AACTCCGCCGGCATGTACCGCGGCTACGTGGGCGAGGACGGCAAGCCCGTCGTGGCCATCTTCCGGGATGCGGCGGAGGC
CGCTGGCGCGAAGTAG

Upstream 100 bases:

>100_bases
GTGCTACCGACGGAGCGCTCTGGAGGTCCTTCGAATGTCCGCCGCTTCCGTTCTCCTCCGCCGTTCTTTCGTCGTGGGTG
CCGTGATGCTGCTTTCTCCC

Downstream 100 bases:

>100_bases
CAGCGCACGGAGGCCACCATCCGGGATGCGCCCGGGGGGCCTCCCCGAGCGCGCCGTGAGGCGTCAGTCCCGGCCGCCGC
GATGTCCCCCATCGCGGCGG

Product: asparaginase family protein

Products: NA

Alternate protein names: Beta-aspartyl-peptidase; EcAIII; Isoaspartyl dipeptidase [H]

Number of amino acids: Translated: 351; Mature: 350

Protein sequence:

>351_residues
MGCATQTGAARTDSEQPTSRDAPAPKPKWGLVIHGGAGVISRENLSPEREEEVRAALTQSLQAGHAVLASGGSALDAVSA
AVRILEDSPYFNAGKGAVFTHDGVNELDAAIMDGTTRKAGAVAGLRHVKNPISLARLVMEQSPHVMMVGEGAEAFAKSQG
VELVDPKYFYTEDRWQGLQRALEKERSQPQPSSALPAGYDPVSGDHKFGTVGAVALDQTGALAAATSTGGMTNKRYGRVG
DSPIIGAGTYADARCAVSATGHGEFFIRYTVARDICARVEYQNVPLPEAADVVINDVLVKVGGEGGVIAMDREGNVAMPF
NSAGMYRGYVGEDGKPVVAIFRDAAEAAGAK

Sequences:

>Translated_351_residues
MGCATQTGAARTDSEQPTSRDAPAPKPKWGLVIHGGAGVISRENLSPEREEEVRAALTQSLQAGHAVLASGGSALDAVSA
AVRILEDSPYFNAGKGAVFTHDGVNELDAAIMDGTTRKAGAVAGLRHVKNPISLARLVMEQSPHVMMVGEGAEAFAKSQG
VELVDPKYFYTEDRWQGLQRALEKERSQPQPSSALPAGYDPVSGDHKFGTVGAVALDQTGALAAATSTGGMTNKRYGRVG
DSPIIGAGTYADARCAVSATGHGEFFIRYTVARDICARVEYQNVPLPEAADVVINDVLVKVGGEGGVIAMDREGNVAMPF
NSAGMYRGYVGEDGKPVVAIFRDAAEAAGAK
>Mature_350_residues
GCATQTGAARTDSEQPTSRDAPAPKPKWGLVIHGGAGVISRENLSPEREEEVRAALTQSLQAGHAVLASGGSALDAVSAA
VRILEDSPYFNAGKGAVFTHDGVNELDAAIMDGTTRKAGAVAGLRHVKNPISLARLVMEQSPHVMMVGEGAEAFAKSQGV
ELVDPKYFYTEDRWQGLQRALEKERSQPQPSSALPAGYDPVSGDHKFGTVGAVALDQTGALAAATSTGGMTNKRYGRVGD
SPIIGAGTYADARCAVSATGHGEFFIRYTVARDICARVEYQNVPLPEAADVVINDVLVKVGGEGGVIAMDREGNVAMPFN
SAGMYRGYVGEDGKPVVAIFRDAAEAAGAK

Specific function: May be involved in glutathione, and possibly other peptide, transport, although these results could also be due to polar effects of disruption [H]

COG id: COG1446

COG function: function code E; Asparaginase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Ntn-hydrolase family [H]

Homologues:

Organism=Homo sapiens, GI145275202, Length=297, Percent_Identity=42.0875420875421, Blast_Score=200, Evalue=1e-51,
Organism=Homo sapiens, GI145275200, Length=297, Percent_Identity=42.0875420875421, Blast_Score=200, Evalue=1e-51,
Organism=Homo sapiens, GI98991769, Length=295, Percent_Identity=30.8474576271186, Blast_Score=115, Evalue=7e-26,
Organism=Homo sapiens, GI285002251, Length=219, Percent_Identity=37.4429223744292, Blast_Score=108, Evalue=9e-24,
Organism=Homo sapiens, GI285002253, Length=219, Percent_Identity=35.1598173515982, Blast_Score=91, Evalue=1e-18,
Organism=Escherichia coli, GI1787050, Length=316, Percent_Identity=53.1645569620253, Blast_Score=306, Evalue=2e-84,
Organism=Caenorhabditis elegans, GI17569239, Length=241, Percent_Identity=28.2157676348548, Blast_Score=85, Evalue=5e-17,
Organism=Drosophila melanogaster, GI18921183, Length=325, Percent_Identity=38.7692307692308, Blast_Score=187, Evalue=8e-48,
Organism=Drosophila melanogaster, GI21357893, Length=277, Percent_Identity=32.8519855595668, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI221330102, Length=240, Percent_Identity=35.4166666666667, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24652135, Length=240, Percent_Identity=35.4166666666667, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24655553, Length=264, Percent_Identity=29.1666666666667, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24657358, Length=235, Percent_Identity=30.2127659574468, Blast_Score=77, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000246 [H]

Pfam domain/function: PF01112 Asparaginase_2 [H]

EC number: =3.4.19.5 [H]

Molecular weight: Translated: 36601; Mature: 36470

Theoretical pI: Translated: 5.43; Mature: 5.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGCATQTGAARTDSEQPTSRDAPAPKPKWGLVIHGGAGVISRENLSPEREEEVRAALTQS
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCEEECCCCCCHHHHHHHHHHHHH
LQAGHAVLASGGSALDAVSAAVRILEDSPYFNAGKGAVFTHDGVNELDAAIMDGTTRKAG
HHCCCEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCCEEECCCHHHHHHHHHCCCCCHHH
AVAGLRHVKNPISLARLVMEQSPHVMMVGEGAEAFAKSQGVELVDPKYFYTEDRWQGLQR
HHHHHHHHCCHHHHHHHHHHCCCCEEEEECCHHHHHHHCCCEEECCCEEECCHHHHHHHH
ALEKERSQPQPSSALPAGYDPVSGDHKFGTVGAVALDQTGALAAATSTGGMTNKRYGRVG
HHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCEEEECCCCCCCCCCCCCCC
DSPIIGAGTYADARCAVSATGHGEFFIRYTVARDICARVEYQNVPLPEAADVVINDVLVK
CCCEECCCCCCCCEEEEEECCCCEEEEEEHHHHHHHHHCCCCCCCCCCHHHHHHEEEEEE
VGGEGGVIAMDREGNVAMPFNSAGMYRGYVGEDGKPVVAIFRDAAEAAGAK
ECCCCCEEEEECCCCEEECCCCCCCEECCCCCCCCEEEEEEHHHHHHCCCC
>Mature Secondary Structure 
GCATQTGAARTDSEQPTSRDAPAPKPKWGLVIHGGAGVISRENLSPEREEEVRAALTQS
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCEEECCCCCCHHHHHHHHHHHHH
LQAGHAVLASGGSALDAVSAAVRILEDSPYFNAGKGAVFTHDGVNELDAAIMDGTTRKAG
HHCCCEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCCEEECCCHHHHHHHHHCCCCCHHH
AVAGLRHVKNPISLARLVMEQSPHVMMVGEGAEAFAKSQGVELVDPKYFYTEDRWQGLQR
HHHHHHHHCCHHHHHHHHHHCCCCEEEEECCHHHHHHHCCCEEECCCEEECCHHHHHHHH
ALEKERSQPQPSSALPAGYDPVSGDHKFGTVGAVALDQTGALAAATSTGGMTNKRYGRVG
HHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCEEEECCCCCCCCCCCCCCC
DSPIIGAGTYADARCAVSATGHGEFFIRYTVARDICARVEYQNVPLPEAADVVINDVLVK
CCCEECCCCCCCCEEEEEECCCCEEEEEEHHHHHHHHHCCCCCCCCCCHHHHHHEEEEEE
VGGEGGVIAMDREGNVAMPFNSAGMYRGYVGEDGKPVVAIFRDAAEAAGAK
ECCCCCEEEEECCCCEEECCCCCCCEECCCCCCCCEEEEEEHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 3045084; 7984428 [H]