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The map label for this gene is crt [H]

Identifier: 108762855

GI number: 108762855

Start: 4474964

End: 4475740

Strand: Direct

Name: crt [H]

Synonym: MXAN_3757

Alternate gene names: 108762855

Gene position: 4474964-4475740 (Clockwise)

Preceding gene: 108760873

Following gene: 108758347

Centisome position: 48.96

GC content: 70.91

Gene sequence:

>777_bases
ATGCCGGAATTCAAGGTCGACGCACGCGGTCCCATCGAAATCTGGACCATCGACGGCGAGAGCCGCCGCAATGCCATCAG
CCGCGCCATGCTGAAGGAGTTGGGCGAGCTGGTGACCCGCGTGTCTTCCAGCCGTGACGTGCGCGCCGTCGTCATCACCG
GCGCGGGCGACAAGGCCTTCTGCGCCGGCGCCGACCTGAAGGAGCGCGCCACCATGGCCGAGGACGAGGTCCGCGCCTTC
CTGGACGGGCTGCGCCGCACCTTCCGCGCGATCGAGAAGAGCGACTGCGTCTTCATCGCCGCCATCAACGGCGCGGCCTT
GGGCGGTGGCACAGAGCTGGCGCTCGCGTGTGATTTGCGCGTGGCCGCCCCGGCCGCGGAGCTGGGTCTCACCGAGGTGA
AGCTGGGCATCATCCCCGGCGGCGGAGGCACGCAGCGCCTGGCGCGCCTGGTGGGCCCGGGCCGCGCGAAGGACCTCATC
CTCACCGCCCGCCGCATCAACGCCGCAGAGGCCTTCAGCGTGGGCCTGGCGAACCGATTGGCGCCGGAGGGGCACCTGCT
GGCCGTGGCGTACGGGTTGGCGGAGTCGGTGGTGGAGAACGCGCCCATCGCCGTGGCCACGGCCAAGCACGCCATCGACG
AGGGCACGGGCCTGGAGCTGGACGATGCGCTGGCGCTGGAGCTGCGCAAGTACGAGGAGATTCTCAAGACGGAGGACCGC
CTGGAGGGCCTGCGCGCCTTCGCGGAGAAGCGCGCGCCCGTCTACAAGGGCCGCTAG

Upstream 100 bases:

>100_bases
CCCATGCTAACCGTTTCCGCCTTGCCAGACCCGGCTTATGGCCGCACTCTGCCGCGGCTCAGAGCAGCCCGCCTGCCAGG
CCGGGAAAAGGGGAGCGAAC

Downstream 100 bases:

>100_bases
CCCGCACGGACGAAATCGGGGACGGAAGGCCCGGTACTCACCGGTGCCCCGTCCCCGAAGTGCGTCACGTCACATGGCGC
GAAGGCCACGCGCGCCGGTC

Product: 3-hydroxybutyryl-CoA dehydratase

Products: trans(or 3)-enoyl-CoA; H2O [C]

Alternate protein names: NA

Number of amino acids: Translated: 258; Mature: 257

Protein sequence:

>258_residues
MPEFKVDARGPIEIWTIDGESRRNAISRAMLKELGELVTRVSSSRDVRAVVITGAGDKAFCAGADLKERATMAEDEVRAF
LDGLRRTFRAIEKSDCVFIAAINGAALGGGTELALACDLRVAAPAAELGLTEVKLGIIPGGGGTQRLARLVGPGRAKDLI
LTARRINAAEAFSVGLANRLAPEGHLLAVAYGLAESVVENAPIAVATAKHAIDEGTGLELDDALALELRKYEEILKTEDR
LEGLRAFAEKRAPVYKGR

Sequences:

>Translated_258_residues
MPEFKVDARGPIEIWTIDGESRRNAISRAMLKELGELVTRVSSSRDVRAVVITGAGDKAFCAGADLKERATMAEDEVRAF
LDGLRRTFRAIEKSDCVFIAAINGAALGGGTELALACDLRVAAPAAELGLTEVKLGIIPGGGGTQRLARLVGPGRAKDLI
LTARRINAAEAFSVGLANRLAPEGHLLAVAYGLAESVVENAPIAVATAKHAIDEGTGLELDDALALELRKYEEILKTEDR
LEGLRAFAEKRAPVYKGR
>Mature_257_residues
PEFKVDARGPIEIWTIDGESRRNAISRAMLKELGELVTRVSSSRDVRAVVITGAGDKAFCAGADLKERATMAEDEVRAFL
DGLRRTFRAIEKSDCVFIAAINGAALGGGTELALACDLRVAAPAAELGLTEVKLGIIPGGGGTQRLARLVGPGRAKDLIL
TARRINAAEAFSVGLANRLAPEGHLLAVAYGLAESVVENAPIAVATAKHAIDEGTGLELDDALALELRKYEEILKTEDRL
EGLRAFAEKRAPVYKGR

Specific function: Could Possibly Oxidizes Fatty Acids Using Specific Components (By Similarity). [C]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI4502327, Length=239, Percent_Identity=45.6066945606695, Blast_Score=167, Evalue=1e-41,
Organism=Homo sapiens, GI194097323, Length=235, Percent_Identity=35.7446808510638, Blast_Score=144, Evalue=6e-35,
Organism=Homo sapiens, GI150378541, Length=242, Percent_Identity=35.9504132231405, Blast_Score=132, Evalue=3e-31,
Organism=Homo sapiens, GI70995211, Length=247, Percent_Identity=30.7692307692308, Blast_Score=105, Evalue=4e-23,
Organism=Homo sapiens, GI20127408, Length=176, Percent_Identity=35.7954545454545, Blast_Score=102, Evalue=3e-22,
Organism=Homo sapiens, GI68989263, Length=157, Percent_Identity=40.1273885350318, Blast_Score=101, Evalue=8e-22,
Organism=Homo sapiens, GI213417737, Length=225, Percent_Identity=26.6666666666667, Blast_Score=89, Evalue=4e-18,
Organism=Homo sapiens, GI157694516, Length=225, Percent_Identity=26.6666666666667, Blast_Score=89, Evalue=5e-18,
Organism=Homo sapiens, GI31542718, Length=243, Percent_Identity=27.1604938271605, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI157694520, Length=202, Percent_Identity=25.2475247524752, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI261878539, Length=75, Percent_Identity=50.6666666666667, Blast_Score=72, Evalue=4e-13,
Organism=Homo sapiens, GI62530384, Length=169, Percent_Identity=28.4023668639053, Blast_Score=72, Evalue=6e-13,
Organism=Escherichia coli, GI1787659, Length=259, Percent_Identity=37.8378378378378, Blast_Score=155, Evalue=4e-39,
Organism=Escherichia coli, GI221142681, Length=241, Percent_Identity=38.5892116182573, Blast_Score=147, Evalue=5e-37,
Organism=Escherichia coli, GI1788597, Length=249, Percent_Identity=30.9236947791165, Blast_Score=106, Evalue=1e-24,
Organism=Escherichia coli, GI1788682, Length=189, Percent_Identity=37.037037037037, Blast_Score=104, Evalue=7e-24,
Organism=Escherichia coli, GI87082183, Length=242, Percent_Identity=28.5123966942149, Blast_Score=101, Evalue=6e-23,
Organism=Escherichia coli, GI1790281, Length=166, Percent_Identity=32.5301204819277, Blast_Score=94, Evalue=9e-21,
Organism=Escherichia coli, GI1787660, Length=251, Percent_Identity=32.2709163346614, Blast_Score=86, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17540714, Length=223, Percent_Identity=39.9103139013453, Blast_Score=172, Evalue=1e-43,
Organism=Caenorhabditis elegans, GI25145438, Length=240, Percent_Identity=37.5, Blast_Score=154, Evalue=6e-38,
Organism=Caenorhabditis elegans, GI17554946, Length=236, Percent_Identity=36.4406779661017, Blast_Score=152, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI17558304, Length=180, Percent_Identity=36.1111111111111, Blast_Score=111, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI17560910, Length=202, Percent_Identity=34.1584158415842, Blast_Score=109, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17508953, Length=184, Percent_Identity=34.7826086956522, Blast_Score=95, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI25144276, Length=184, Percent_Identity=34.7826086956522, Blast_Score=95, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17536985, Length=250, Percent_Identity=28.4, Blast_Score=95, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17508951, Length=184, Percent_Identity=34.7826086956522, Blast_Score=95, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17534483, Length=221, Percent_Identity=27.6018099547511, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI25144160, Length=209, Percent_Identity=30.622009569378, Blast_Score=83, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI25144157, Length=209, Percent_Identity=30.622009569378, Blast_Score=83, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI25141363, Length=166, Percent_Identity=29.5180722891566, Blast_Score=74, Evalue=8e-14,
Organism=Caenorhabditis elegans, GI17540306, Length=244, Percent_Identity=28.2786885245902, Blast_Score=71, Evalue=6e-13,
Organism=Drosophila melanogaster, GI24653139, Length=254, Percent_Identity=44.0944881889764, Blast_Score=202, Evalue=1e-52,
Organism=Drosophila melanogaster, GI20129971, Length=229, Percent_Identity=38.4279475982533, Blast_Score=153, Evalue=1e-37,
Organism=Drosophila melanogaster, GI24653477, Length=229, Percent_Identity=38.4279475982533, Blast_Score=153, Evalue=1e-37,
Organism=Drosophila melanogaster, GI24650670, Length=264, Percent_Identity=32.5757575757576, Blast_Score=92, Evalue=3e-19,
Organism=Drosophila melanogaster, GI19920382, Length=206, Percent_Identity=32.5242718446602, Blast_Score=92, Evalue=3e-19,
Organism=Drosophila melanogaster, GI21357171, Length=185, Percent_Identity=35.1351351351351, Blast_Score=90, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24654903, Length=193, Percent_Identity=26.9430051813472, Blast_Score=89, Evalue=4e-18,
Organism=Drosophila melanogaster, GI28571729, Length=159, Percent_Identity=30.8176100628931, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI28571730, Length=159, Percent_Identity=30.8176100628931, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24583165, Length=160, Percent_Identity=31.875, Blast_Score=72, Evalue=4e-13,
Organism=Drosophila melanogaster, GI19921018, Length=159, Percent_Identity=30.8176100628931, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: 4.2.1.17 [C]

Molecular weight: Translated: 27401; Mature: 27270

Theoretical pI: Translated: 5.96; Mature: 5.96

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPEFKVDARGPIEIWTIDGESRRNAISRAMLKELGELVTRVSSSRDVRAVVITGAGDKAF
CCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCE
CAGADLKERATMAEDEVRAFLDGLRRTFRAIEKSDCVFIAAINGAALGGGTELALACDLR
ECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCEECCCCEEEEEEEEE
VAAPAAELGLTEVKLGIIPGGGGTQRLARLVGPGRAKDLILTARRINAAEAFSVGLANRL
EECCHHHCCCEEEEEEEEECCCHHHHHHHHHCCCCCHHHEEEHHHCCHHHHHHHHHHHHC
APEGHLLAVAYGLAESVVENAPIAVATAKHAIDEGTGLELDDALALELRKYEEILKTEDR
CCCCCEEEHHHHHHHHHHHCCCEEEEEHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHH
LEGLRAFAEKRAPVYKGR
HHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
PEFKVDARGPIEIWTIDGESRRNAISRAMLKELGELVTRVSSSRDVRAVVITGAGDKAF
CCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCE
CAGADLKERATMAEDEVRAFLDGLRRTFRAIEKSDCVFIAAINGAALGGGTELALACDLR
ECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCEECCCCEEEEEEEEE
VAAPAAELGLTEVKLGIIPGGGGTQRLARLVGPGRAKDLILTARRINAAEAFSVGLANRL
EECCHHHCCCEEEEEEEEECCCHHHHHHHHHCCCCCHHHEEEHHHCCHHHHHHHHHHHHC
APEGHLLAVAYGLAESVVENAPIAVATAKHAIDEGTGLELDDALALELRKYEEILKTEDR
CCCCCEEEHHHHHHHHHHHCCCEEEEEHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHH
LEGLRAFAEKRAPVYKGR
HHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.05 {crotonyl-CoA}} 0.008 {2-decenoyl-CoA}} [C]

Substrates: (3S)-hydroxyacyl-CoA [C]

Specific reaction: (3S)-hydroxyacyl-CoA = trans(or 3)-enoyl-CoA + H2O [C]

General reaction: addition (of H2O to carbon carbon double bondelimination (of H2O C-O bond cleavagehydration [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9387222; 9384377 [H]