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The map label for this gene is rimK [H]

Identifier: 108762539

GI number: 108762539

Start: 3590588

End: 3591589

Strand: Direct

Name: rimK [H]

Synonym: MXAN_3061

Alternate gene names: 108762539

Gene position: 3590588-3591589 (Clockwise)

Preceding gene: 108760924

Following gene: 108757864

Centisome position: 39.29

GC content: 69.96

Gene sequence:

>1002_bases
ATGAAAGCCACGATCCTCTCGCGCTCCGCTTCCATCCCGTCCACTCGGCGACTTGTCGAGGTGGCGCGCGCAAGAGGGCA
CCGGGTGCGGGTCCTCAACCCACTCCGCGTGCAGATGCACCTCGATGGGCGCAGCGCCACGCTCTACTACGGCCGCAAGA
AGCTGGCGCCCACGGACGTCGTCCTCCCCCGCATCGCCCAGTCCATCAGTAACTATGGCCTGGCCGTGGTGAATCAGTTC
GGCCTGGCGCGGGTCTCGCTCGTCAACCACGCGCAGGCCATCGCGCAGTCGCGCAACAAGATGCGCTCGCTGCAACTGCT
CTCGGCGCACGGCATCGACATTCCGTCGACGGTGATGGCGCGCGACGCGGCTCACCTCAAGGAGATGGTGGGCTTGCTGG
GCGGCGTGCCCGTCCTCGTCAAACTGCTGCAGGGGCAGGAGAAGCACGGCGTCATGGTGTGCGAGAGCCTCCAGTCGCTG
GAGGCCGCGCTCGAGGCCGTCCTGGGGCTGGGGCACAATCTCGTGATGCAGGAGTACGTGAAGAGCACGGGCATCGACGT
CCGTGTGCTCGTCGTGGGTGGGCAGGCGGTGGCCGCGGTTCGTCGCCGCCCGCGCCCCGGGCGCCTGGCGCACACCCTCA
TCAAGGGGGCCCGGCTGGAGGCGCACGAGCTGTCACCCGCCCAGCGAGCCACCGCGGAGAAGGCCACCCGGCTCATCGGG
CTCGAAGTGGCGGCGGTGGACCTGCTCGACGTGCAGGGCCAACCCAAGATCTTCGAGGTGAACAGCTCCCCCGCGCTTCC
TGAGATGGAGGCGGTGACGGGCGTGGACCTGGCCTCGCTCATCATCATCCGCGCGGAAGCGCTCGTGGCGGGGGAGCCGC
CCGTGTCCGTGCCGGACCTGTCGCCTCCGTCGGCCCTGCCCGCGTCATCTGAGCCTGCCCAGCTCCCTGTGGACCGGAAG
GGCACGGGGCGCCGCGCTCGGACCGGCGAAGGCGGCGCGTGA

Upstream 100 bases:

>100_bases
GGCCATCCGTCAGCGCTAGTCCAGCGGTTCGCGGGGCGGCTTTACCCGGCGAGGGGCGGGGATTATAGGTCCCTCTCTCC
CCGGAACGGCCGCCCCGCTC

Downstream 100 bases:

>100_bases
AGAGAAAGACGGCCCCCGCTGTCTCAAGCGTGATACGCACCCATCGTTTCCAGGAGACGCCCATGCTTCGTCGCCAACGC
CTTTCCTCCCTCGCCGCCCT

Product: alpha-L-glutamate ligase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 333; Mature: 333

Protein sequence:

>333_residues
MKATILSRSASIPSTRRLVEVARARGHRVRVLNPLRVQMHLDGRSATLYYGRKKLAPTDVVLPRIAQSISNYGLAVVNQF
GLARVSLVNHAQAIAQSRNKMRSLQLLSAHGIDIPSTVMARDAAHLKEMVGLLGGVPVLVKLLQGQEKHGVMVCESLQSL
EAALEAVLGLGHNLVMQEYVKSTGIDVRVLVVGGQAVAAVRRRPRPGRLAHTLIKGARLEAHELSPAQRATAEKATRLIG
LEVAAVDLLDVQGQPKIFEVNSSPALPEMEAVTGVDLASLIIIRAEALVAGEPPVSVPDLSPPSALPASSEPAQLPVDRK
GTGRRARTGEGGA

Sequences:

>Translated_333_residues
MKATILSRSASIPSTRRLVEVARARGHRVRVLNPLRVQMHLDGRSATLYYGRKKLAPTDVVLPRIAQSISNYGLAVVNQF
GLARVSLVNHAQAIAQSRNKMRSLQLLSAHGIDIPSTVMARDAAHLKEMVGLLGGVPVLVKLLQGQEKHGVMVCESLQSL
EAALEAVLGLGHNLVMQEYVKSTGIDVRVLVVGGQAVAAVRRRPRPGRLAHTLIKGARLEAHELSPAQRATAEKATRLIG
LEVAAVDLLDVQGQPKIFEVNSSPALPEMEAVTGVDLASLIIIRAEALVAGEPPVSVPDLSPPSALPASSEPAQLPVDRK
GTGRRARTGEGGA
>Mature_333_residues
MKATILSRSASIPSTRRLVEVARARGHRVRVLNPLRVQMHLDGRSATLYYGRKKLAPTDVVLPRIAQSISNYGLAVVNQF
GLARVSLVNHAQAIAQSRNKMRSLQLLSAHGIDIPSTVMARDAAHLKEMVGLLGGVPVLVKLLQGQEKHGVMVCESLQSL
EAALEAVLGLGHNLVMQEYVKSTGIDVRVLVVGGQAVAAVRRRPRPGRLAHTLIKGARLEAHELSPAQRATAEKATRLIG
LEVAAVDLLDVQGQPKIFEVNSSPALPEMEAVTGVDLASLIIIRAEALVAGEPPVSVPDLSPPSALPASSEPAQLPVDRK
GTGRRARTGEGGA

Specific function: Responsible for the addition of glutamate residues to the C-terminus of ribosomal protein S6 [H]

COG id: COG0189

COG function: function code HJ; Glutathione synthase/Ribosomal protein S6 modification enzyme (glutaminyl transferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1787076, Length=283, Percent_Identity=38.5159010600707, Blast_Score=208, Evalue=4e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013651
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR004666 [H]

Pfam domain/function: PF08443 RimK [H]

EC number: NA

Molecular weight: Translated: 35392; Mature: 35392

Theoretical pI: Translated: 10.92; Mature: 10.92

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKATILSRSASIPSTRRLVEVARARGHRVRVLNPLRVQMHLDGRSATLYYGRKKLAPTDV
CCCCEECCCCCCCHHHHHHHHHHHCCCEEEEECCEEEEEEECCCCEEEEECCCCCCCHHH
VLPRIAQSISNYGLAVVNQFGLARVSLVNHAQAIAQSRNKMRSLQLLSAHGIDIPSTVMA
HHHHHHHHHHHCCHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
RDAAHLKEMVGLLGGVPVLVKLLQGQEKHGVMVCESLQSLEAALEAVLGLGHNLVMQEYV
HHHHHHHHHHHHHCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
KSTGIDVRVLVVGGQAVAAVRRRPRPGRLAHTLIKGARLEAHELSPAQRATAEKATRLIG
HHCCCCEEEEEECCHHHHHHHCCCCCCHHHHHHHHCCCCCHHHCCHHHHHHHHHHHHHHH
LEVAAVDLLDVQGQPKIFEVNSSPALPEMEAVTGVDLASLIIIRAEALVAGEPPVSVPDL
HHHHEEEEEECCCCCCEEEECCCCCCCCHHHHHCHHHHHHHHHHHHHHCCCCCCCCCCCC
SPPSALPASSEPAQLPVDRKGTGRRARTGEGGA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKATILSRSASIPSTRRLVEVARARGHRVRVLNPLRVQMHLDGRSATLYYGRKKLAPTDV
CCCCEECCCCCCCHHHHHHHHHHHCCCEEEEECCEEEEEEECCCCEEEEECCCCCCCHHH
VLPRIAQSISNYGLAVVNQFGLARVSLVNHAQAIAQSRNKMRSLQLLSAHGIDIPSTVMA
HHHHHHHHHHHCCHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
RDAAHLKEMVGLLGGVPVLVKLLQGQEKHGVMVCESLQSLEAALEAVLGLGHNLVMQEYV
HHHHHHHHHHHHHCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
KSTGIDVRVLVVGGQAVAAVRRRPRPGRLAHTLIKGARLEAHELSPAQRATAEKATRLIG
HHCCCCEEEEEECCHHHHHHHCCCCCCHHHHHHHHCCCCCHHHCCHHHHHHHHHHHHHHH
LEVAAVDLLDVQGQPKIFEVNSSPALPEMEAVTGVDLASLIIIRAEALVAGEPPVSVPDL
HHHHEEEEEECCCCCCEEEECCCCCCCCHHHHHCHHHHHHHHHHHHHHCCCCCCCCCCCC
SPPSALPASSEPAQLPVDRKGTGRRARTGEGGA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Glu; Ribosomal protein S6 [C]

Specific reaction: Catalyzes the addition of glutamic acids to the carboxyl end of ribosomal protein S6 [C]

General reaction: Ligases; Forming Carbon-Nitrogen Bonds; Acid-D-Amino-Acid Ligases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA