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The map label for this gene is thiG

Identifier: 108762312

GI number: 108762312

Start: 3820820

End: 3821605

Strand: Reverse

Name: thiG

Synonym: MXAN_3268

Alternate gene names: 108762312

Gene position: 3821605-3820820 (Counterclockwise)

Preceding gene: 108763282

Following gene: 108760708

Centisome position: 41.81

GC content: 68.45

Gene sequence:

>786_bases
ATGAGCATCCAGGACAAGCCTTTCACCATCGCCGGAGTGACGTTCAGCTCCCGGCTCATCCTCGGCACGGGGAAGTATCC
CAGCCACGACATCATGAAGCGCTGCCACGAGTCCTCCGGCACGGAGATGGTGACCGTGGCCGTGCGCCGCCTGGACCTCA
AGGCCACGGGCGAGGCGTCGCTGATGAACTGGATTGACCGCAACCGCCTGCGCCTGCTCCCCAACACAGCGCTCTGCTAC
ACGGCGGATGACGCGGTCCGCACCTGCCGGCTCGCCGAGGAGCTGGGCATGAGCAAGTGGGTGAAGCTCGAGGTGCTCGG
CGACGAGAAGACGCTCTACCCGGACGTCGAGGAGACGGTGAAGGCGGCCCGCATCCTGGTGAAGGAGGGCTTCACCGTGC
TGCCGTACACCAGCGACGACCCCATCACCGCCCGCAAGCTGGAGGACGCGGGCTGCGCGGCCGTGATGCCGCTGGCGGCG
CCCATCGGCAGCGGCCTGGGCATCCGCAATCCGCACAACATCCGCCTCATCCTGGAGACGGTGAAGGTCCCCGTCATCGT
GGACGCGGGCGTGGGCACGGCGTCCGACGCGGCCATCGCCATGGAGTTGGGCGTGGAGGCGGTGCTGATGAACACCGCCA
TCGCCGGAGCGCAGGACCCGGTGCGCATGGCCGTGGCCATGAAGAAGGCGGTGGAGGCTGGCCGCGACGCGTATCTGGCG
GGCCGCATCCCGCGAAAGGCCTACGGCTCCGCGTCCAGCCCGATTGACGGGCTCGTCCACCACTGA

Upstream 100 bases:

>100_bases
AGGTGGTGCGCCGCGCCCGCCATCCCGAACACCACCTCCAGGCGGGAGACCGCGTGGAAATCGTCACCTTCGTCGGCGGC
GGCTAGCCAGGAGTCCGCAC

Downstream 100 bases:

>100_bases
CGGTCGGGGGCGCACGGTGGTGCCTTCAACCGTTCCCCGGCTCGTCGTCATCACCGACTGGCGCCTGCCGTCCGAACGGC
TGAGGTGGGCGCTCACCCGG

Product: thiazole synthase

Products: 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate; 4-hydroxy-benzyl-alcohol; C1 of tyrosine; ThiS protein [C]

Alternate protein names: NA

Number of amino acids: Translated: 261; Mature: 260

Protein sequence:

>261_residues
MSIQDKPFTIAGVTFSSRLILGTGKYPSHDIMKRCHESSGTEMVTVAVRRLDLKATGEASLMNWIDRNRLRLLPNTALCY
TADDAVRTCRLAEELGMSKWVKLEVLGDEKTLYPDVEETVKAARILVKEGFTVLPYTSDDPITARKLEDAGCAAVMPLAA
PIGSGLGIRNPHNIRLILETVKVPVIVDAGVGTASDAAIAMELGVEAVLMNTAIAGAQDPVRMAVAMKKAVEAGRDAYLA
GRIPRKAYGSASSPIDGLVHH

Sequences:

>Translated_261_residues
MSIQDKPFTIAGVTFSSRLILGTGKYPSHDIMKRCHESSGTEMVTVAVRRLDLKATGEASLMNWIDRNRLRLLPNTALCY
TADDAVRTCRLAEELGMSKWVKLEVLGDEKTLYPDVEETVKAARILVKEGFTVLPYTSDDPITARKLEDAGCAAVMPLAA
PIGSGLGIRNPHNIRLILETVKVPVIVDAGVGTASDAAIAMELGVEAVLMNTAIAGAQDPVRMAVAMKKAVEAGRDAYLA
GRIPRKAYGSASSPIDGLVHH
>Mature_260_residues
SIQDKPFTIAGVTFSSRLILGTGKYPSHDIMKRCHESSGTEMVTVAVRRLDLKATGEASLMNWIDRNRLRLLPNTALCYT
ADDAVRTCRLAEELGMSKWVKLEVLGDEKTLYPDVEETVKAARILVKEGFTVLPYTSDDPITARKLEDAGCAAVMPLAAP
IGSGLGIRNPHNIRLILETVKVPVIVDAGVGTASDAAIAMELGVEAVLMNTAIAGAQDPVRMAVAMKKAVEAGRDAYLAG
RIPRKAYGSASSPIDGLVHH

Specific function: Catalyzes the rearrangement of 1-deoxy-D-xylulose 5- phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H(2)S

COG id: COG2022

COG function: function code H; Uncharacterized enzyme of thiazole biosynthesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiG family

Homologues:

Organism=Escherichia coli, GI48994993, Length=250, Percent_Identity=46, Blast_Score=211, Evalue=4e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIG_MYXXD (Q1D7A6)

Other databases:

- EMBL:   CP000113
- RefSeq:   YP_631467.1
- ProteinModelPortal:   Q1D7A6
- SMR:   Q1D7A6
- STRING:   Q1D7A6
- GeneID:   4106058
- GenomeReviews:   CP000113_GR
- KEGG:   mxa:MXAN_3268
- TIGR:   MXAN_3268
- eggNOG:   COG2022
- HOGENOM:   HBG296821
- OMA:   VAIRRTN
- PhylomeDB:   Q1D7A6
- ProtClustDB:   PRK00208
- BioCyc:   MXAN246197:MXAN_3268-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00443
- InterPro:   IPR013785
- InterPro:   IPR008867
- Gene3D:   G3DSA:3.20.20.70

Pfam domain/function: PF05690 ThiG; SSF110399 ThiG

EC number: NA

Molecular weight: Translated: 27997; Mature: 27866

Theoretical pI: Translated: 7.50; Mature: 7.50

Prosite motif: NA

Important sites: ACT_SITE 102-102 BINDING 163-163

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIQDKPFTIAGVTFSSRLILGTGKYPSHDIMKRCHESSGTEMVTVAVRRLDLKATGEAS
CCCCCCCEEEEEEEEECEEEEECCCCCCHHHHHHHHHCCCCEEEEEEEHHHCCCCCCCHH
LMNWIDRNRLRLLPNTALCYTADDAVRTCRLAEELGMSKWVKLEVLGDEKTLYPDVEETV
HHHHHHCCCEEECCCCEEEEECHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCHHHHH
KAARILVKEGFTVLPYTSDDPITARKLEDAGCAAVMPLAAPIGSGLGIRNPHNIRLILET
HHHHHHHHCCCEEEEECCCCCCCHHHHCCCCCEEEHHHHHHCCCCCCCCCCCCEEEEEEE
VKVPVIVDAGVGTASDAAIAMELGVEAVLMNTAIAGAQDPVRMAVAMKKAVEAGRDAYLA
CCEEEEEECCCCCCCCCEEEEHHCHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCEEEE
GRIPRKAYGSASSPIDGLVHH
CCCCHHHCCCCCCCCHHHCCC
>Mature Secondary Structure 
SIQDKPFTIAGVTFSSRLILGTGKYPSHDIMKRCHESSGTEMVTVAVRRLDLKATGEAS
CCCCCCEEEEEEEEECEEEEECCCCCCHHHHHHHHHCCCCEEEEEEEHHHCCCCCCCHH
LMNWIDRNRLRLLPNTALCYTADDAVRTCRLAEELGMSKWVKLEVLGDEKTLYPDVEETV
HHHHHHCCCEEECCCCEEEEECHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCHHHHH
KAARILVKEGFTVLPYTSDDPITARKLEDAGCAAVMPLAAPIGSGLGIRNPHNIRLILET
HHHHHHHHCCCEEEEECCCCCCCHHHHCCCCCEEEHHHHHHCCCCCCCCCCCCEEEEEEE
VKVPVIVDAGVGTASDAAIAMELGVEAVLMNTAIAGAQDPVRMAVAMKKAVEAGRDAYLA
CCEEEEEECCCCCCCCCEEEEHHCHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCEEEE
GRIPRKAYGSASSPIDGLVHH
CCCCHHHCCCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: deoxyxylulose-5-phosphate; ThiS-COSH; L-tyrosine [C]

Specific reaction: deoxyxylulose-5-phosphate + ThiS-COSH + L-tyrosine = 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate + 4-hydroxy-benzyl-alcohol + C1 of tyrosine + ThiS protein [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA