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The map label for this gene is hflB [C]

Identifier: 108761830

GI number: 108761830

Start: 7059246

End: 7061003

Strand: Reverse

Name: hflB [C]

Synonym: MXAN_5695

Alternate gene names: 108761830

Gene position: 7061003-7059246 (Counterclockwise)

Preceding gene: 108761001

Following gene: 108758616

Centisome position: 77.26

GC content: 67.24

Gene sequence:

>1758_bases
GTGAGCAAGGTGCGCAAGGTGAACAAGGCGGATCCCCTGGCGGATCTTCCTCGCTGGGCCCAGCAGTTGGCCCGGAAGTA
CTACACGAAGACGGTCAGCGCCTTCCTGCTGTACGGGGCCGTGCGGGACTTGCAGCCCCTGCAGCTCGAGGAGGGCGGCC
GCGGCTTCGGCACGCTGAAGACGTTCCTCTCCGAGGAGCTCTTCGGCGGCCGGGACCACGTCATCTTCTATGACCGGTCG
TCCGGCATCCGCTCCGCGACGCCGGAGACGCAGAAGGACCTGCAGCGGGCCATGGCGGGCTACGACGCCATGTACGGCAC
GGACTACGCCAAGGTCATGCCGAGAGACCCGGGCCGCGCGCTCCAGATTCTGGAGAACTTCATGCGCATGCGCCTGAGCG
AGGGCCGCTCGCTGGCGCTCATCGTCGACTTCGCGGAGACGCTCGTTCCCGGCGGAGAGATGAGCCACCTTTCCAGCGAG
GACCGCTTCGTGGTGGCCACGTTGGACAAGTGGGCGCACGATCCGCAGTTCCTCGCGTCCGACGTGTCCGTGGTGCTGCT
GGCGGAGAACCTGGCGGACGTGTCGCCGCGCATCAGCCGCAACCCGTACGTGGCGCCCATCGAGCTGCCCCTTCCCACCG
AGGAGGAGCGGCTGGAGTACGTGCGCTACAAGCTGGAGGGCAAGCGGCTCCAGTCCGTGTCGGACGTGCCGCTGGCGGGC
CTGGCGAAGATGACGGCGGGTTTGTCCCGCATCAACCTGGACCGCGTGCTCACCGAGGCGCTCGAGCGCGAGGTGCGCAT
CACCTCCGAGCTGCTCAAGGAGAAGAAGCGCGAAATCATCCAGGCGGAGTGCCACGGCCTGCTGGAGTTCATCGAGCCGG
TGCACACGCTGGACGCCGTGGCGGGACACGCCAAGGCCAAGCAGATGCTGCGGCAGGCCGCCGGGGCCCTGAAGAAGGGG
CGCCTGGAGGTCATGCCCATGGGCTACCTGCTGTCGGGCCCCGTGGGCACGGGCAAGACGTTCATGGTGAGCTGCTTCGC
CGGGGAGATTGGAATCCCCGTGGTGAAGTTCCTCAACTTCCGCAGCCAGTGGCAGGGCGTCACCGAGGCGAACCTCGAGA
AGATCTTCAACCTGCTCAAGGCCCTGTGGCCGGTGGCGGTGATGATTGACGAGGCGGATACGTTCCTCGGCAACCGTGAC
TCCGGCGGAGACTCCGGGACGAGCAGCCGCGTGTTCGGCTCCATCGCCTCGTTCATGGGCAACACGTTCTACCGCGGGAA
GATTGTCTGGTTCCTGATGACGGCGCGGCCGGACCTGCTGCCCATCGACCTCAAGCGGCAGGGGCGCGCGGAGGAGCACC
TGGCGCTCTTCTATCCGCAGACGGACGCGGAGCGGGACGAGCTGTTCCAGGTCATGTCCAAGAAGACGGGCGTGTCCGTG
GAAGGCATCGAATCCTTCTCCACGCTGATTCCGGAGGGCGTGCGCGCCTTCAGCGGCGCGGACATCGAAGCCGTCATGGT
GCGCTCGAAGTTCCGCGCGCTGGCGGAGGGCCGCGAGGCGGTGACGAAGGACGACCTGGCCGCGGTGCTCGCGGACTTCG
TGCCCCCCAGCTACCCGCTGGAAATCGAGCTGCAGAACCTGGTGGCGGTGCAGGAGTGCACCAGCCGCGAGCTGCTGCCC
GAGGGCTTCCGCCAACTGGACCGGGACTACATCACCCGGCGCGTGCGCGAGCTGAAGGCGCTGCTGGAGTCGCAGTAG

Upstream 100 bases:

>100_bases
CAAGCGGCAGCCGCGCTGCCGTTAGCCGAAGGCCCGCTTCCCGAGAAGGGGGCGGGCGCGCCAGCTCCCCTGTGGGCGGA
GCGAGAGGAGGCGGGAGTTC

Downstream 100 bases:

>100_bases
CCGGTGCTGCCTTACGCCCAGGCGCTTGTCCTGGGCGCACGGCCCGCTGAGACTTGGGACATGGCTGATGGCCCCACTGC
TCCGCGTTCCTGGTTCGCGT

Product: AAA family ATPase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 585; Mature: 584

Protein sequence:

>585_residues
MSKVRKVNKADPLADLPRWAQQLARKYYTKTVSAFLLYGAVRDLQPLQLEEGGRGFGTLKTFLSEELFGGRDHVIFYDRS
SGIRSATPETQKDLQRAMAGYDAMYGTDYAKVMPRDPGRALQILENFMRMRLSEGRSLALIVDFAETLVPGGEMSHLSSE
DRFVVATLDKWAHDPQFLASDVSVVLLAENLADVSPRISRNPYVAPIELPLPTEEERLEYVRYKLEGKRLQSVSDVPLAG
LAKMTAGLSRINLDRVLTEALEREVRITSELLKEKKREIIQAECHGLLEFIEPVHTLDAVAGHAKAKQMLRQAAGALKKG
RLEVMPMGYLLSGPVGTGKTFMVSCFAGEIGIPVVKFLNFRSQWQGVTEANLEKIFNLLKALWPVAVMIDEADTFLGNRD
SGGDSGTSSRVFGSIASFMGNTFYRGKIVWFLMTARPDLLPIDLKRQGRAEEHLALFYPQTDAERDELFQVMSKKTGVSV
EGIESFSTLIPEGVRAFSGADIEAVMVRSKFRALAEGREAVTKDDLAAVLADFVPPSYPLEIELQNLVAVQECTSRELLP
EGFRQLDRDYITRRVRELKALLESQ

Sequences:

>Translated_585_residues
MSKVRKVNKADPLADLPRWAQQLARKYYTKTVSAFLLYGAVRDLQPLQLEEGGRGFGTLKTFLSEELFGGRDHVIFYDRS
SGIRSATPETQKDLQRAMAGYDAMYGTDYAKVMPRDPGRALQILENFMRMRLSEGRSLALIVDFAETLVPGGEMSHLSSE
DRFVVATLDKWAHDPQFLASDVSVVLLAENLADVSPRISRNPYVAPIELPLPTEEERLEYVRYKLEGKRLQSVSDVPLAG
LAKMTAGLSRINLDRVLTEALEREVRITSELLKEKKREIIQAECHGLLEFIEPVHTLDAVAGHAKAKQMLRQAAGALKKG
RLEVMPMGYLLSGPVGTGKTFMVSCFAGEIGIPVVKFLNFRSQWQGVTEANLEKIFNLLKALWPVAVMIDEADTFLGNRD
SGGDSGTSSRVFGSIASFMGNTFYRGKIVWFLMTARPDLLPIDLKRQGRAEEHLALFYPQTDAERDELFQVMSKKTGVSV
EGIESFSTLIPEGVRAFSGADIEAVMVRSKFRALAEGREAVTKDDLAAVLADFVPPSYPLEIELQNLVAVQECTSRELLP
EGFRQLDRDYITRRVRELKALLESQ
>Mature_584_residues
SKVRKVNKADPLADLPRWAQQLARKYYTKTVSAFLLYGAVRDLQPLQLEEGGRGFGTLKTFLSEELFGGRDHVIFYDRSS
GIRSATPETQKDLQRAMAGYDAMYGTDYAKVMPRDPGRALQILENFMRMRLSEGRSLALIVDFAETLVPGGEMSHLSSED
RFVVATLDKWAHDPQFLASDVSVVLLAENLADVSPRISRNPYVAPIELPLPTEEERLEYVRYKLEGKRLQSVSDVPLAGL
AKMTAGLSRINLDRVLTEALEREVRITSELLKEKKREIIQAECHGLLEFIEPVHTLDAVAGHAKAKQMLRQAAGALKKGR
LEVMPMGYLLSGPVGTGKTFMVSCFAGEIGIPVVKFLNFRSQWQGVTEANLEKIFNLLKALWPVAVMIDEADTFLGNRDS
GGDSGTSSRVFGSIASFMGNTFYRGKIVWFLMTARPDLLPIDLKRQGRAEEHLALFYPQTDAERDELFQVMSKKTGVSVE
GIESFSTLIPEGVRAFSGADIEAVMVRSKFRALAEGREAVTKDDLAAVLADFVPPSYPLEIELQNLVAVQECTSRELLPE
GFRQLDRDYITRRVRELKALLESQ

Specific function: Seems To Act As An ATP-Dependent Zinc Metallopeptidase. Involved In The Degradation Of Sigma-32. Degrades Carboxy- Terminal-Tagged Cytoplasmic Proteins. These Proteins Are Tagged With An 11-Amino-Acid Nonpolar Destabilizing Tail VIa A Mechanism Involving

COG id: COG0465

COG function: function code O; ATP-dependent Zn proteases

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AAA ATPase family. CDC48 subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657689, Length=256, Percent_Identity=27.734375, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI21327685, Length=256, Percent_Identity=27.734375, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI157671927, Length=154, Percent_Identity=30.5194805194805, Blast_Score=69, Evalue=1e-11,
Organism=Escherichia coli, GI1789568, Length=186, Percent_Identity=27.9569892473118, Blast_Score=66, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI25153574, Length=561, Percent_Identity=23.8859180035651, Blast_Score=80, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17508053, Length=326, Percent_Identity=24.5398773006135, Blast_Score=73, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17532375, Length=417, Percent_Identity=23.5011990407674, Blast_Score=70, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17537217, Length=197, Percent_Identity=26.9035532994924, Blast_Score=69, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6323429, Length=198, Percent_Identity=29.2929292929293, Blast_Score=80, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6320077, Length=414, Percent_Identity=24.8792270531401, Blast_Score=79, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6322994, Length=280, Percent_Identity=26.4285714285714, Blast_Score=76, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6323736, Length=235, Percent_Identity=30.2127659574468, Blast_Score=72, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6320854, Length=221, Percent_Identity=27.1493212669683, Blast_Score=70, Evalue=8e-13,
Organism=Saccharomyces cerevisiae, GI6324833, Length=205, Percent_Identity=27.8048780487805, Blast_Score=69, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6322651, Length=196, Percent_Identity=28.0612244897959, Blast_Score=65, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6324000, Length=202, Percent_Identity=28.2178217821782, Blast_Score=64, Evalue=5e-11,
Organism=Drosophila melanogaster, GI281365776, Length=222, Percent_Identity=27.4774774774775, Blast_Score=75, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24660075, Length=222, Percent_Identity=27.4774774774775, Blast_Score=75, Evalue=2e-13,
Organism=Drosophila melanogaster, GI45552965, Length=222, Percent_Identity=27.4774774774775, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI19921202, Length=436, Percent_Identity=24.3119266055046, Blast_Score=71, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24639391, Length=272, Percent_Identity=27.9411764705882, Blast_Score=70, Evalue=4e-12,
Organism=Drosophila melanogaster, GI17137560, Length=197, Percent_Identity=26.3959390862944, Blast_Score=69, Evalue=7e-12,
Organism=Drosophila melanogaster, GI161076488, Length=421, Percent_Identity=22.5653206650831, Blast_Score=69, Evalue=7e-12,
Organism=Drosophila melanogaster, GI161076486, Length=197, Percent_Identity=26.3959390862944, Blast_Score=69, Evalue=8e-12,
Organism=Drosophila melanogaster, GI24652279, Length=197, Percent_Identity=26.3959390862944, Blast_Score=67, Evalue=4e-11,
Organism=Drosophila melanogaster, GI24658770, Length=241, Percent_Identity=25.7261410788382, Blast_Score=66, Evalue=8e-11,
Organism=Drosophila melanogaster, GI21355121, Length=193, Percent_Identity=27.4611398963731, Blast_Score=66, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009010
- InterPro:   IPR003593
- InterPro:   IPR005938
- InterPro:   IPR003959
- InterPro:   IPR003960
- InterPro:   IPR003338
- InterPro:   IPR004201
- InterPro:   IPR015415 [H]

Pfam domain/function: PF00004 AAA; PF02933 CDC48_2; PF02359 CDC48_N; PF09336 Vps4_C [H]

EC number: 3.4.24.- [C]

Molecular weight: Translated: 65307; Mature: 65176

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKVRKVNKADPLADLPRWAQQLARKYYTKTVSAFLLYGAVRDLQPLQLEEGGRGFGTLK
CCCHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCHHHHH
TFLSEELFGGRDHVIFYDRSSGIRSATPETQKDLQRAMAGYDAMYGTDYAKVMPRDPGRA
HHHHHHHCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCHH
LQILENFMRMRLSEGRSLALIVDFAETLVPGGEMSHLSSEDRFVVATLDKWAHDPQFLAS
HHHHHHHHHHHHHCCCEEEEEEEHHHHHCCCCCHHCCCCCCCEEEEEHHHHCCCHHHHHC
DVSVVLLAENLADVSPRISRNPYVAPIELPLPTEEERLEYVRYKLEGKRLQSVSDVPLAG
CHHHEEEEHHHHHCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCHHHCCCCCCCHHH
LAKMTAGLSRINLDRVLTEALEREVRITSELLKEKKREIIQAECHGLLEFIEPVHTLDAV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AGHAKAKQMLRQAAGALKKGRLEVMPMGYLLSGPVGTGKTFMVSCFAGEIGIPVVKFLNF
HHHHHHHHHHHHHHHHHHCCCEEEEECCEEEECCCCCCCCEEEEEECCCCCHHHHHHHHH
RSQWQGVTEANLEKIFNLLKALWPVAVMIDEADTFLGNRDSGGDSGTSSRVFGSIASFMG
HHHHCCCCHHHHHHHHHHHHHHHHHHEEEECHHHHCCCCCCCCCCCCHHHHHHHHHHHHC
NTFYRGKIVWFLMTARPDLLPIDLKRQGRAEEHLALFYPQTDAERDELFQVMSKKTGVSV
CCEECCEEEEEEEECCCCCEEECCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHCCCCH
EGIESFSTLIPEGVRAFSGADIEAVMVRSKFRALAEGREAVTKDDLAAVLADFVPPSYPL
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCE
EIELQNLVAVQECTSRELLPEGFRQLDRDYITRRVRELKALLESQ
EEEHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SKVRKVNKADPLADLPRWAQQLARKYYTKTVSAFLLYGAVRDLQPLQLEEGGRGFGTLK
CCHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCHHHHH
TFLSEELFGGRDHVIFYDRSSGIRSATPETQKDLQRAMAGYDAMYGTDYAKVMPRDPGRA
HHHHHHHCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCHH
LQILENFMRMRLSEGRSLALIVDFAETLVPGGEMSHLSSEDRFVVATLDKWAHDPQFLAS
HHHHHHHHHHHHHCCCEEEEEEEHHHHHCCCCCHHCCCCCCCEEEEEHHHHCCCHHHHHC
DVSVVLLAENLADVSPRISRNPYVAPIELPLPTEEERLEYVRYKLEGKRLQSVSDVPLAG
CHHHEEEEHHHHHCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCHHHCCCCCCCHHH
LAKMTAGLSRINLDRVLTEALEREVRITSELLKEKKREIIQAECHGLLEFIEPVHTLDAV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AGHAKAKQMLRQAAGALKKGRLEVMPMGYLLSGPVGTGKTFMVSCFAGEIGIPVVKFLNF
HHHHHHHHHHHHHHHHHHCCCEEEEECCEEEECCCCCCCCEEEEEECCCCCHHHHHHHHH
RSQWQGVTEANLEKIFNLLKALWPVAVMIDEADTFLGNRDSGGDSGTSSRVFGSIASFMG
HHHHCCCCHHHHHHHHHHHHHHHHHHEEEECHHHHCCCCCCCCCCCCHHHHHHHHHHHHC
NTFYRGKIVWFLMTARPDLLPIDLKRQGRAEEHLALFYPQTDAERDELFQVMSKKTGVSV
CCEECCEEEEEEEECCCCCEEECCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHCCCCH
EGIESFSTLIPEGVRAFSGADIEAVMVRSKFRALAEGREAVTKDDLAAVLADFVPPSYPL
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCE
EIELQNLVAVQECTSRELLPEGFRQLDRDYITRRVRELKALLESQ
EEEHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Metalloendopeptidases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9389475 [H]