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The map label for this gene is eno

Identifier: 108761804

GI number: 108761804

Start: 5511805

End: 5513103

Strand: Reverse

Name: eno

Synonym: MXAN_4451

Alternate gene names: 108761804

Gene position: 5513103-5511805 (Counterclockwise)

Preceding gene: 108761313

Following gene: 108760040

Centisome position: 60.32

GC content: 68.51

Gene sequence:

>1299_bases
ATGACCGAGATTTCTCAGATTCTGGCGCGCGAAGTGCTCGACTCCCGTGGCAACCCGACCGTGGAGGCGGAGGTCCAGCT
TGCCGGGGGCGCTCGTGGCCGCGCCGCGGTGCCGTCCGGTGCGTCCACCGGTGAGCATGAGGCCATCGAGCTGCGCGACG
GCGACAAGCATCGCTACCTGGGCAAGGGCGTGCAGAAGGCCGTGAAGAACGTGGTCGACGTGCTCGCGCCCGCGCTGGTG
GGCATGGACGCGGCGGATCAGTTCGCGGTGGACCAGCGGATGCTGGAGCTGGACGGCACCGCCACCAAGGGCAAGCTGGG
CGCCAACGCCATCCTCGCGGTGTCCATGGCGGCCGCTCGCGCCGCGGCGGACGCGCACGGGCTGCCGCTGTACCGCTACG
TGGGCGGCGTGCAGGCGCGCACCCTGCCGGTGCCGCTGATGAACATCCTCAACGGCGGCGCGCACGCGGACACCCGCGTG
GACGTGCAGGAGTTCATGGTGGTGCCCGCCGGTGCGTCCTCCTTCGCGGAGGGCCTGCGCTGGGGCGCCGAGGTGTTCCA
CGCGCTGAAGAAGATTCTCAAGGGCCGCAAGCTGGCCACGGGCGTGGGCGACGAGGGCGGCTACGCCCCGGACCTGCCGG
CGAACGAAGAGGCGCTGAAGCTCATCATGGAGGCCATCGACCAGGCGGGCTTCAAGGCCGGTGAGCAGCTCTTCCTGGCC
CTGGACGTGGCGGCCAGCGAGTTCTTCGACAAGGGCAGCAAGAAGTACAAGCTGAAGGGCGAGGGCAAGGAGTACGACTC
GACCGGCCTGCTCGAGTACTACCGGGGCCTCTCCGAGCGCTACCCCATCATCTCCATCGAAGACGGCATGGCGGAGGATG
ACTGGGAGGGCTGGAAGAAGCTCACCGACGCGCTGGGTTCGCGCATGCAGTTGGTGGGTGACGACCTCTTCGTCACCAAC
GTGGAGCGCCTGGGCCGCGGCATCGAGACGGGCACGGCCAACTCCATCCTGGTGAAGGTGAACCAGATTGGCACCCTGAC
GGAGACGTTCGACGCCGTGCGCATGGCGCACCGCGCCGGTTACACGTCCGTCATGAGCCACCGCTCCGGCGAGACGGAGG
ACACCACCATCGCCGACCTGGCCGTGGCGCTGGACTGCGGGCAGATCAAGACGGGTTCCGCGTCCCGCTCCGACCGCGTG
GCCAAGTACAACCAGTTGCTGCGCATCGAAGGCGAGCTGGGGGCCGCGGCCCGCTACGCTGGCAAGTCGGTGTTCCGCGC
CTTGAACCAGAAGAAGTGA

Upstream 100 bases:

>100_bases
GCGGCGTTGGGGCGGAGGCATTCGACGGGTGGACACTGTAGCAGCGCGGGCCGCGAGCACGCTTGCCCGACCCGGAGGGC
TCTTCTAGAAACGGCAGCCT

Downstream 100 bases:

>100_bases
GCCCGTGAGCAACAAGCCCAAGCGCATCCTGGTTTCCAACGACGACGGCTATTTCTCAGAGGGGCTTCAGGCGCTGGTCG
AGGCCGTGAGCCCCCTGGGG

Product: phosphopyruvate hydratase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase

Number of amino acids: Translated: 432; Mature: 431

Protein sequence:

>432_residues
MTEISQILAREVLDSRGNPTVEAEVQLAGGARGRAAVPSGASTGEHEAIELRDGDKHRYLGKGVQKAVKNVVDVLAPALV
GMDAADQFAVDQRMLELDGTATKGKLGANAILAVSMAAARAAADAHGLPLYRYVGGVQARTLPVPLMNILNGGAHADTRV
DVQEFMVVPAGASSFAEGLRWGAEVFHALKKILKGRKLATGVGDEGGYAPDLPANEEALKLIMEAIDQAGFKAGEQLFLA
LDVAASEFFDKGSKKYKLKGEGKEYDSTGLLEYYRGLSERYPIISIEDGMAEDDWEGWKKLTDALGSRMQLVGDDLFVTN
VERLGRGIETGTANSILVKVNQIGTLTETFDAVRMAHRAGYTSVMSHRSGETEDTTIADLAVALDCGQIKTGSASRSDRV
AKYNQLLRIEGELGAAARYAGKSVFRALNQKK

Sequences:

>Translated_432_residues
MTEISQILAREVLDSRGNPTVEAEVQLAGGARGRAAVPSGASTGEHEAIELRDGDKHRYLGKGVQKAVKNVVDVLAPALV
GMDAADQFAVDQRMLELDGTATKGKLGANAILAVSMAAARAAADAHGLPLYRYVGGVQARTLPVPLMNILNGGAHADTRV
DVQEFMVVPAGASSFAEGLRWGAEVFHALKKILKGRKLATGVGDEGGYAPDLPANEEALKLIMEAIDQAGFKAGEQLFLA
LDVAASEFFDKGSKKYKLKGEGKEYDSTGLLEYYRGLSERYPIISIEDGMAEDDWEGWKKLTDALGSRMQLVGDDLFVTN
VERLGRGIETGTANSILVKVNQIGTLTETFDAVRMAHRAGYTSVMSHRSGETEDTTIADLAVALDCGQIKTGSASRSDRV
AKYNQLLRIEGELGAAARYAGKSVFRALNQKK
>Mature_431_residues
TEISQILAREVLDSRGNPTVEAEVQLAGGARGRAAVPSGASTGEHEAIELRDGDKHRYLGKGVQKAVKNVVDVLAPALVG
MDAADQFAVDQRMLELDGTATKGKLGANAILAVSMAAARAAADAHGLPLYRYVGGVQARTLPVPLMNILNGGAHADTRVD
VQEFMVVPAGASSFAEGLRWGAEVFHALKKILKGRKLATGVGDEGGYAPDLPANEEALKLIMEAIDQAGFKAGEQLFLAL
DVAASEFFDKGSKKYKLKGEGKEYDSTGLLEYYRGLSERYPIISIEDGMAEDDWEGWKKLTDALGSRMQLVGDDLFVTNV
ERLGRGIETGTANSILVKVNQIGTLTETFDAVRMAHRAGYTSVMSHRSGETEDTTIADLAVALDCGQIKTGSASRSDRVA
KYNQLLRIEGELGAAARYAGKSVFRALNQKK

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the bacterial ce

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family

Homologues:

Organism=Homo sapiens, GI5803011, Length=435, Percent_Identity=51.7241379310345, Blast_Score=421, Evalue=1e-118,
Organism=Homo sapiens, GI301897477, Length=433, Percent_Identity=50.5773672055427, Blast_Score=415, Evalue=1e-116,
Organism=Homo sapiens, GI301897469, Length=433, Percent_Identity=50.5773672055427, Blast_Score=415, Evalue=1e-116,
Organism=Homo sapiens, GI4503571, Length=433, Percent_Identity=49.1916859122402, Blast_Score=411, Evalue=1e-115,
Organism=Homo sapiens, GI301897479, Length=431, Percent_Identity=45.9396751740139, Blast_Score=360, Evalue=2e-99,
Organism=Homo sapiens, GI169201331, Length=336, Percent_Identity=27.0833333333333, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI169201757, Length=336, Percent_Identity=27.0833333333333, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI239744207, Length=336, Percent_Identity=27.0833333333333, Blast_Score=110, Evalue=2e-24,
Organism=Escherichia coli, GI1789141, Length=427, Percent_Identity=58.7822014051522, Blast_Score=486, Evalue=1e-138,
Organism=Caenorhabditis elegans, GI71995829, Length=436, Percent_Identity=51.8348623853211, Blast_Score=416, Evalue=1e-116,
Organism=Caenorhabditis elegans, GI17536383, Length=436, Percent_Identity=51.8348623853211, Blast_Score=416, Evalue=1e-116,
Organism=Caenorhabditis elegans, GI32563855, Length=197, Percent_Identity=49.746192893401, Blast_Score=184, Evalue=5e-47,
Organism=Saccharomyces cerevisiae, GI6321693, Length=435, Percent_Identity=51.9540229885057, Blast_Score=399, Evalue=1e-112,
Organism=Saccharomyces cerevisiae, GI6323985, Length=433, Percent_Identity=49.4226327944573, Blast_Score=393, Evalue=1e-110,
Organism=Saccharomyces cerevisiae, GI6324974, Length=433, Percent_Identity=49.4226327944573, Blast_Score=393, Evalue=1e-110,
Organism=Saccharomyces cerevisiae, GI6324969, Length=433, Percent_Identity=49.4226327944573, Blast_Score=393, Evalue=1e-110,
Organism=Saccharomyces cerevisiae, GI6321968, Length=435, Percent_Identity=51.7241379310345, Blast_Score=372, Evalue=1e-104,
Organism=Drosophila melanogaster, GI24580918, Length=434, Percent_Identity=48.6175115207373, Blast_Score=391, Evalue=1e-109,
Organism=Drosophila melanogaster, GI24580916, Length=434, Percent_Identity=48.6175115207373, Blast_Score=391, Evalue=1e-109,
Organism=Drosophila melanogaster, GI24580920, Length=434, Percent_Identity=48.6175115207373, Blast_Score=391, Evalue=1e-109,
Organism=Drosophila melanogaster, GI24580914, Length=434, Percent_Identity=48.6175115207373, Blast_Score=391, Evalue=1e-109,
Organism=Drosophila melanogaster, GI281360527, Length=434, Percent_Identity=48.6175115207373, Blast_Score=390, Evalue=1e-109,
Organism=Drosophila melanogaster, GI17137654, Length=434, Percent_Identity=48.6175115207373, Blast_Score=390, Evalue=1e-109,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): ENO_MYXXD (Q1D401)

Other databases:

- EMBL:   CP000113
- RefSeq:   YP_632622.1
- ProteinModelPortal:   Q1D401
- SMR:   Q1D401
- STRING:   Q1D401
- GeneID:   4105547
- GenomeReviews:   CP000113_GR
- KEGG:   mxa:MXAN_4451
- TIGR:   MXAN_4451
- eggNOG:   COG0148
- HOGENOM:   HBG726599
- OMA:   DIAVGTN
- PhylomeDB:   Q1D401
- ProtClustDB:   PRK00077
- BioCyc:   MXAN246197:MXAN_4451-MONOMER
- GO:   GO:0006096
- HAMAP:   MF_00318
- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811
- PIRSF:   PIRSF001400
- PRINTS:   PR00148
- TIGRFAMs:   TIGR01060

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N

EC number: =4.2.1.11

Molecular weight: Translated: 46145; Mature: 46014

Theoretical pI: Translated: 5.75; Mature: 5.75

Prosite motif: PS00164 ENOLASE

Important sites: ACT_SITE 205-205 ACT_SITE 339-339 BINDING 155-155 BINDING 164-164 BINDING 287-287 BINDING 314-314 BINDING 339-339 BINDING 390-390

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEISQILAREVLDSRGNPTVEAEVQLAGGARGRAAVPSGASTGEHEAIELRDGDKHRYL
CCHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCEEEECCCCCHHHH
GKGVQKAVKNVVDVLAPALVGMDAADQFAVDQRMLELDGTATKGKLGANAILAVSMAAAR
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHH
AAADAHGLPLYRYVGGVQARTLPVPLMNILNGGAHADTRVDVQEFMVVPAGASSFAEGLR
HHHCCCCCHHHHHHCCCCCEECCHHHHHHHCCCCCCCCCCCHHHHEEECCCHHHHHHHHH
WGAEVFHALKKILKGRKLATGVGDEGGYAPDLPANEEALKLIMEAIDQAGFKAGEQLFLA
HHHHHHHHHHHHHCCCCEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEE
LDVAASEFFDKGSKKYKLKGEGKEYDSTGLLEYYRGLSERYPIISIEDGMAEDDWEGWKK
HHHHHHHHHHCCCCEEEECCCCCCCCCCHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHH
LTDALGSRMQLVGDDLFVTNVERLGRGIETGTANSILVKVNQIGTLTETFDAVRMAHRAG
HHHHHHHHHHHHCCCHHEECHHHHCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHCC
YTSVMSHRSGETEDTTIADLAVALDCGQIKTGSASRSDRVAKYNQLLRIEGELGAAARYA
HHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHEEECCCCCHHHHHH
GKSVFRALNQKK
HHHHHHHHHCCC
>Mature Secondary Structure 
TEISQILAREVLDSRGNPTVEAEVQLAGGARGRAAVPSGASTGEHEAIELRDGDKHRYL
CHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCEEEECCCCCHHHH
GKGVQKAVKNVVDVLAPALVGMDAADQFAVDQRMLELDGTATKGKLGANAILAVSMAAAR
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHH
AAADAHGLPLYRYVGGVQARTLPVPLMNILNGGAHADTRVDVQEFMVVPAGASSFAEGLR
HHHCCCCCHHHHHHCCCCCEECCHHHHHHHCCCCCCCCCCCHHHHEEECCCHHHHHHHHH
WGAEVFHALKKILKGRKLATGVGDEGGYAPDLPANEEALKLIMEAIDQAGFKAGEQLFLA
HHHHHHHHHHHHHCCCCEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEE
LDVAASEFFDKGSKKYKLKGEGKEYDSTGLLEYYRGLSERYPIISIEDGMAEDDWEGWKK
HHHHHHHHHHCCCCEEEECCCCCCCCCCHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHH
LTDALGSRMQLVGDDLFVTNVERLGRGIETGTANSILVKVNQIGTLTETFDAVRMAHRAG
HHHHHHHHHHHHCCCHHEECHHHHCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHCC
YTSVMSHRSGETEDTTIADLAVALDCGQIKTGSASRSDRVAKYNQLLRIEGELGAAARYA
HHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHEEECCCCCHHHHHH
GKSVFRALNQKK
HHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA