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The map label for this gene is nla28 [H]

Identifier: 108761633

GI number: 108761633

Start: 1361810

End: 1363153

Strand: Direct

Name: nla28 [H]

Synonym: MXAN_1167

Alternate gene names: 108761633

Gene position: 1361810-1363153 (Clockwise)

Preceding gene: 108760911

Following gene: 108759629

Centisome position: 14.9

GC content: 70.76

Gene sequence:

>1344_bases
ATGAGCTCAGCCCGCATCCTCGTCGTGGATGATGACCCGCAGGCCCGCGATTTGCTCCAGCGCTTGCTGGGGCCGCTGGG
GGCGGTGACGCAGGCTCCGGACCCGCGCCGCGCCACCGAGCGCATCGCGGAGGGCGCCTTTGACCTGGTCCTCACGGACA
TGGCCATGCCCGAGCCGGGCGACGGGCTGAAGGTGCTCCATGAGGTGAAGGCGCAACTGCCGGACACGCCCGTGGTGGTG
GTGACAGCCTTCGGCAACATCGAGGGCGCGCTGGACAGCATCCAGCAGGGCGCCTTCGACTACCTGGCGAAGCCCTTCGA
CGTGGACGCGATTCTGCGCGTGGCGCGCCGGGCGCTGGAGCAGAAGCGGCTGGTGGAGGAGAACCGCTCGCTGCGCCAGC
AGGTGGAGCGCGGTTCGCTGATGCTGGTGGGCCGCAGCCCGGCGCTGCTGGAGGTGTACAAGCACGTGGCGCGCGCGGCG
GCCAGCAACGTGCCAGTGCTGATTACGGGCGAGACGGGCACGGGGAAGGAGATGGTGGCCCGCGCGCTGCACAAGCGCTC
GCCGCGCATGAACGGGCCCTTCATCCCCGTGGACTGCGGCGCGATTACGGAGTCGCTGATGGAGAGCGAACTGTTCGGCC
ACGCGAAGGGCAGCTTCACCGGCGCGTCCGGCGCGCGGCGAGGCGTCTTCGAGGAGGCCAGCGGCGGCACGCTCTTCCTG
GATGAGATTGGCGACGTCGGCATGAAGGTACAGTCGCAGCTCCTGCGCGTGTTGCAGGAGGGCGAAATCCGCCGCGTGGG
TGAGAGCGTCCCCGTCAAGGTGGACGTGCGCGTGGTGGCGGCGACGAACAAGGACCTCAAGACGCGGGTGGCGGAAGGCC
TCTTCCGCGAGGACCTGCTGTACCGCCTGGACGTGGTGCACCTGCACCTGCCACCGCTGCGGGAGCGGAGCGAGGACATC
CCCGCGCTGGTGGAGCACTTCGCCGGGCGACATGCGCGCGGCGGCGTGCGGCCGGTGGTGACGTCGGAGGCGAACGCGCG
CCTGGCGGCCTACGACTGGCCGGGCAACGTGCGGCAGTTGGAGAACGTGGTGGCGCGGGCGCTGGCGCTCAACGTGACGG
GCGTCCTGGGGCCCCAGGACTTCCCGGAGCCCATCGGGGATGCGTCCACCCCGAAGATGGCGGGCCTGGCGGGCGACATG
CCGAGTCTGGCCGAGCTGTCCCGCCGCTACGCCGCGCAGGTGCTCCAGCACGTGGGCGGCAACAAGAGCGAGGCGGCGCG
CCTGCTCGATGTGGACCGCAAGACGCTCTACAAGCTGCTGGAGGCCCCGGAGGCCGAGTCGTAG

Upstream 100 bases:

>100_bases
CGCACGTGGTGGTGCGCTTCCCCCGCGTGGTGCGCCCGGCGGCGGGCACCAGCGGTTCTCAGACAACGACTGCTTCCGCC
GGAATGCGCAAGGTCGGAAC

Downstream 100 bases:

>100_bases
CGCGCGCGGCGGACGTCAGTTGCTGACGCCGAGCAGCTCCCGAATCTGCCGCAGCATCTCGATTTCGGCCGCGTGCACGC
TGCCGTCGCTGGCGATGAGC

Product: sigma-54 dependent DNA-binding reponse regulator Nla28

Products: NA

Alternate protein names: Ornithine decarboxylase antizyme; Ornithine/arginine decarboxylase inhibitor [H]

Number of amino acids: Translated: 447; Mature: 446

Protein sequence:

>447_residues
MSSARILVVDDDPQARDLLQRLLGPLGAVTQAPDPRRATERIAEGAFDLVLTDMAMPEPGDGLKVLHEVKAQLPDTPVVV
VTAFGNIEGALDSIQQGAFDYLAKPFDVDAILRVARRALEQKRLVEENRSLRQQVERGSLMLVGRSPALLEVYKHVARAA
ASNVPVLITGETGTGKEMVARALHKRSPRMNGPFIPVDCGAITESLMESELFGHAKGSFTGASGARRGVFEEASGGTLFL
DEIGDVGMKVQSQLLRVLQEGEIRRVGESVPVKVDVRVVAATNKDLKTRVAEGLFREDLLYRLDVVHLHLPPLRERSEDI
PALVEHFAGRHARGGVRPVVTSEANARLAAYDWPGNVRQLENVVARALALNVTGVLGPQDFPEPIGDASTPKMAGLAGDM
PSLAELSRRYAAQVLQHVGGNKSEAARLLDVDRKTLYKLLEAPEAES

Sequences:

>Translated_447_residues
MSSARILVVDDDPQARDLLQRLLGPLGAVTQAPDPRRATERIAEGAFDLVLTDMAMPEPGDGLKVLHEVKAQLPDTPVVV
VTAFGNIEGALDSIQQGAFDYLAKPFDVDAILRVARRALEQKRLVEENRSLRQQVERGSLMLVGRSPALLEVYKHVARAA
ASNVPVLITGETGTGKEMVARALHKRSPRMNGPFIPVDCGAITESLMESELFGHAKGSFTGASGARRGVFEEASGGTLFL
DEIGDVGMKVQSQLLRVLQEGEIRRVGESVPVKVDVRVVAATNKDLKTRVAEGLFREDLLYRLDVVHLHLPPLRERSEDI
PALVEHFAGRHARGGVRPVVTSEANARLAAYDWPGNVRQLENVVARALALNVTGVLGPQDFPEPIGDASTPKMAGLAGDM
PSLAELSRRYAAQVLQHVGGNKSEAARLLDVDRKTLYKLLEAPEAES
>Mature_446_residues
SSARILVVDDDPQARDLLQRLLGPLGAVTQAPDPRRATERIAEGAFDLVLTDMAMPEPGDGLKVLHEVKAQLPDTPVVVV
TAFGNIEGALDSIQQGAFDYLAKPFDVDAILRVARRALEQKRLVEENRSLRQQVERGSLMLVGRSPALLEVYKHVARAAA
SNVPVLITGETGTGKEMVARALHKRSPRMNGPFIPVDCGAITESLMESELFGHAKGSFTGASGARRGVFEEASGGTLFLD
EIGDVGMKVQSQLLRVLQEGEIRRVGESVPVKVDVRVVAATNKDLKTRVAEGLFREDLLYRLDVVHLHLPPLRERSEDIP
ALVEHFAGRHARGGVRPVVTSEANARLAAYDWPGNVRQLENVVARALALNVTGVLGPQDFPEPIGDASTPKMAGLAGDMP
SLAELSRRYAAQVLQHVGGNKSEAARLLDVDRKTLYKLLEAPEAES

Specific function: Member of the two-component regulatory system AtoS/AtoC involved in the transcriptional regulation of the ato genes for acetoacetate metabolism. Also an inhibitor of polyamine biosynthesis [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=449, Percent_Identity=42.3162583518931, Blast_Score=356, Evalue=2e-99,
Organism=Escherichia coli, GI1790437, Length=434, Percent_Identity=44.9308755760369, Blast_Score=344, Evalue=5e-96,
Organism=Escherichia coli, GI1790299, Length=467, Percent_Identity=42.1841541755889, Blast_Score=337, Evalue=1e-93,
Organism=Escherichia coli, GI1788905, Length=453, Percent_Identity=40.6181015452539, Blast_Score=301, Evalue=4e-83,
Organism=Escherichia coli, GI87082117, Length=255, Percent_Identity=51.3725490196078, Blast_Score=251, Evalue=9e-68,
Organism=Escherichia coli, GI87082152, Length=310, Percent_Identity=43.8709677419355, Blast_Score=238, Evalue=7e-64,
Organism=Escherichia coli, GI1789087, Length=263, Percent_Identity=48.6692015209125, Blast_Score=238, Evalue=8e-64,
Organism=Escherichia coli, GI1789233, Length=226, Percent_Identity=48.6725663716814, Blast_Score=224, Evalue=1e-59,
Organism=Escherichia coli, GI1786524, Length=324, Percent_Identity=41.6666666666667, Blast_Score=221, Evalue=5e-59,
Organism=Escherichia coli, GI87081872, Length=244, Percent_Identity=48.3606557377049, Blast_Score=221, Evalue=1e-58,
Organism=Escherichia coli, GI1787583, Length=289, Percent_Identity=42.9065743944637, Blast_Score=220, Evalue=1e-58,
Organism=Escherichia coli, GI1789828, Length=244, Percent_Identity=35.2459016393443, Blast_Score=144, Evalue=1e-35,
Organism=Escherichia coli, GI87081858, Length=292, Percent_Identity=32.8767123287671, Blast_Score=144, Evalue=1e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 48397; Mature: 48266

Theoretical pI: Translated: 6.27; Mature: 6.27

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSARILVVDDDPQARDLLQRLLGPLGAVTQAPDPRRATERIAEGAFDLVLTDMAMPEPG
CCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCC
DGLKVLHEVKAQLPDTPVVVVTAFGNIEGALDSIQQGAFDYLAKPFDVDAILRVARRALE
CHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
QKRLVEENRSLRQQVERGSLMLVGRSPALLEVYKHVARAAASNVPVLITGETGTGKEMVA
HHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHH
RALHKRSPRMNGPFIPVDCGAITESLMESELFGHAKGSFTGASGARRGVFEEASGGTLFL
HHHHHCCCCCCCCEEECCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEH
DEIGDVGMKVQSQLLRVLQEGEIRRVGESVPVKVDVRVVAATNKDLKTRVAEGLFREDLL
HHHCCHHHHHHHHHHHHHHCCCHHHCCCCCCEEEEEEEEEECCCHHHHHHHHHHHHHHHH
YRLDVVHLHLPPLRERSEDIPALVEHFAGRHARGGVRPVVTSEANARLAAYDWPGNVRQL
HHHHHHEECCCCHHHCCCCHHHHHHHHCCCCCCCCCCCEEECCCCCEEEEECCCCCHHHH
ENVVARALALNVTGVLGPQDFPEPIGDASTPKMAGLAGDMPSLAELSRRYAAQVLQHVGG
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHCCCCCCCHHHHHHHHHHHHHHHHHCC
NKSEAARLLDVDRKTLYKLLEAPEAES
CHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SSARILVVDDDPQARDLLQRLLGPLGAVTQAPDPRRATERIAEGAFDLVLTDMAMPEPG
CCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCC
DGLKVLHEVKAQLPDTPVVVVTAFGNIEGALDSIQQGAFDYLAKPFDVDAILRVARRALE
CHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
QKRLVEENRSLRQQVERGSLMLVGRSPALLEVYKHVARAAASNVPVLITGETGTGKEMVA
HHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHH
RALHKRSPRMNGPFIPVDCGAITESLMESELFGHAKGSFTGASGARRGVFEEASGGTLFL
HHHHHCCCCCCCCEEECCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEH
DEIGDVGMKVQSQLLRVLQEGEIRRVGESVPVKVDVRVVAATNKDLKTRVAEGLFREDLL
HHHCCHHHHHHHHHHHHHHCCCHHHCCCCCCEEEEEEEEEECCCHHHHHHHHHHHHHHHH
YRLDVVHLHLPPLRERSEDIPALVEHFAGRHARGGVRPVVTSEANARLAAYDWPGNVRQL
HHHHHHEECCCCHHHCCCCHHHHHHHHCCCCCCCCCCCEEECCCCCEEEEECCCCCHHHH
ENVVARALALNVTGVLGPQDFPEPIGDASTPKMAGLAGDMPSLAELSRRYAAQVLQHVGG
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHCCCCCCCHHHHHHHHHHHHHHHHHCC
NKSEAARLLDVDRKTLYKLLEAPEAES
CHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503; 8346225 [H]