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The map label for this gene is amsH [H]

Identifier: 108761277

GI number: 108761277

Start: 3775550

End: 3776122

Strand: Direct

Name: amsH [H]

Synonym: MXAN_3225

Alternate gene names: 108761277

Gene position: 3775550-3776122 (Clockwise)

Preceding gene: 108764041

Following gene: 108762066

Centisome position: 41.31

GC content: 68.41

Gene sequence:

>573_bases
ATGGGCAAGACGAGCGCGGGGTTCTGGACGGTGTTGGGCGTGATGCTCCTGGGGGGCTGCGCGCACCAGTCGCAGGTGAA
GGTGGACAACTCGGAGCAGCCCTACCGGATTGGCCGCGAGGACGTGCTGGACATCGCGGTGTGGCGTGACGCGGAGTTGT
CCCGGACGCTGCCGGTCCGTCCGGATGGCTACATCTCCATGCCCATGGTGGGCGAGGTCCACGCCGCGGGCAAGACGCCC
ACGGAGCTGGCCGACTCGCTGAAGGAAGCCTTCCAGCCGTACGTGCAGGAGCCGCGCGTGACGGTGATTGTCCGCGAGGT
GAACAGCAGCCGCGTGTTCGTCACCGGCGAGGTGGCCAACCCGGGCGCCTATCCGCTGCGCGGCCGCGTGTCGCTGCTGC
AGGCCATCGCGCTGGCGGGCGGCTTCACGGACTTCGCCAACTCGGACGGCATCGTGGTCATCCGCACCGACAGCAAGGGC
GGACAGATTCCGGTGCGCTACAGCGACCTCATCTCTCCGGATGGCGGACACGAGGTCATGCTCCGGCCGGGTGACACCGT
CGTCGTTCCGTGA

Upstream 100 bases:

>100_bases
AGTACGTGGCACACGCGCTGCTAAGGCAACGCCGCCGGAAGCAACGCCCGGCCGCGGTAGGGCCGGGAGCGCGACACACG
ATTTTGAGGGAGGGCTGACG

Downstream 100 bases:

>100_bases
GGGATGGTGACGCAGTGGGCGTCGTGGACGTGGGCGGTGGTTGGCGTGCGGGACGGAGGGCGACGGTGAAGGGCAACTGG
AAGAAGTGGCTACTCGCGGG

Product: polysaccharide biosynthesis/export protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 190; Mature: 189

Protein sequence:

>190_residues
MGKTSAGFWTVLGVMLLGGCAHQSQVKVDNSEQPYRIGREDVLDIAVWRDAELSRTLPVRPDGYISMPMVGEVHAAGKTP
TELADSLKEAFQPYVQEPRVTVIVREVNSSRVFVTGEVANPGAYPLRGRVSLLQAIALAGGFTDFANSDGIVVIRTDSKG
GQIPVRYSDLISPDGGHEVMLRPGDTVVVP

Sequences:

>Translated_190_residues
MGKTSAGFWTVLGVMLLGGCAHQSQVKVDNSEQPYRIGREDVLDIAVWRDAELSRTLPVRPDGYISMPMVGEVHAAGKTP
TELADSLKEAFQPYVQEPRVTVIVREVNSSRVFVTGEVANPGAYPLRGRVSLLQAIALAGGFTDFANSDGIVVIRTDSKG
GQIPVRYSDLISPDGGHEVMLRPGDTVVVP
>Mature_189_residues
GKTSAGFWTVLGVMLLGGCAHQSQVKVDNSEQPYRIGREDVLDIAVWRDAELSRTLPVRPDGYISMPMVGEVHAAGKTPT
ELADSLKEAFQPYVQEPRVTVIVREVNSSRVFVTGEVANPGAYPLRGRVSLLQAIALAGGFTDFANSDGIVVIRTDSKGG
QIPVRYSDLISPDGGHEVMLRPGDTVVVP

Specific function: Involved in the biosynthesis of amylovoran which functions as a virulence factor. Might be involved in the translocation of polysaccharides across the outer membrane [H]

COG id: COG1596

COG function: function code M; Periplasmic protein involved in polysaccharide export

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the BexD/CtrA/VexA family [H]

Homologues:

Organism=Escherichia coli, GI1788376, Length=173, Percent_Identity=30.0578034682081, Blast_Score=73, Evalue=1e-14,
Organism=Escherichia coli, GI1787218, Length=171, Percent_Identity=30.9941520467836, Blast_Score=70, Evalue=9e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003715
- InterPro:   IPR019554 [H]

Pfam domain/function: PF02563 Poly_export; PF10531 SLBB [H]

EC number: NA

Molecular weight: Translated: 20433; Mature: 20301

Theoretical pI: Translated: 5.26; Mature: 5.26

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKTSAGFWTVLGVMLLGGCAHQSQVKVDNSEQPYRIGREDVLDIAVWRDAELSRTLPVR
CCCCCCHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCHHEEEEEEECCCCCCCCCCCC
PDGYISMPMVGEVHAAGKTPTELADSLKEAFQPYVQEPRVTVIVREVNSSRVFVTGEVAN
CCCEEECCCCCEEECCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCEEEEEEECCC
PGAYPLRGRVSLLQAIALAGGFTDFANSDGIVVIRTDSKGGQIPVRYSDLISPDGGHEVM
CCCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCEEEEEHHHCCCCCCCCEEE
LRPGDTVVVP
EECCCEEEEC
>Mature Secondary Structure 
GKTSAGFWTVLGVMLLGGCAHQSQVKVDNSEQPYRIGREDVLDIAVWRDAELSRTLPVR
CCCCCHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCHHEEEEEEECCCCCCCCCCCC
PDGYISMPMVGEVHAAGKTPTELADSLKEAFQPYVQEPRVTVIVREVNSSRVFVTGEVAN
CCCEEECCCCCEEECCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCEEEEEEECCC
PGAYPLRGRVSLLQAIALAGGFTDFANSDGIVVIRTDSKGGQIPVRYSDLISPDGGHEVM
CCCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCEEEEEHHHCCCCCCCCEEE
LRPGDTVVVP
EECCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7596293 [H]