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The map label for this gene is gltB [H]

Identifier: 108761021

GI number: 108761021

Start: 4715095

End: 4716555

Strand: Direct

Name: gltB [H]

Synonym: MXAN_3918

Alternate gene names: 108761021

Gene position: 4715095-4716555 (Clockwise)

Preceding gene: 108760738

Following gene: 108762200

Centisome position: 51.59

GC content: 71.87

Gene sequence:

>1461_bases
ATGGGTAAGCCAACTGGATTCATCGAGTGGGAGCGCGTCCACGCGGACAAGCGGGACAAGGCGGACCGGCTGGGCGACTG
GCGCGAGTTCGCGCTGCCGCTGGCCCCCGACGAGGCCAAACGGCAGGCCGGCCGCTGCATGGACTGTGGCGTCCCCTTCT
GCCACCAGGGCTGTCCCCTGGGGAACCTCATCCCCGACTTCAACGAGGCCGTGTACCGCGGGCGCTGGCGCGAGGCGTAT
GACTTGCTCAGCCGCACCAATGGCTTCCCGGAGATGACGGGCCGGCTGTGCCCGGCGCCGTGCGAGGCCGCGTGTGTGCT
CGCCATCGACCGGGACCCGGTCACCATCGAACAGATGGAGAAGGAAATCGTCGAACGGGCCTTCACGGAGGGCTGGGTGA
AGCCCCGGCCTCCGGCACGCCGCACCGGGAAGACGGTGGGTGTGGTGGGCTCCGGGCCCGCGGGACTGGCGGCGGCGGCG
CAGCTCAACGCGGCCGGACACACCGTCACCGTGTACGAGCGGGATGCCCGGGCCGGCGGCCTGCTGCGCTATGGCATCCC
CGACTTCAAGCTGGAGAAGTCCGTGGTGGACCGGCGCCTGGCGCTGATGGAGGCGGAGGGCATCACCTTCGTCACCGGCG
TGGACGTGGGCGGCGCCCTGAGCTACCGCGAGCTGCGCGCGAAGCATGACGCCCTGGTGCTGGCCATGGGCGCGCGCCGT
CCGCGCGAGCTGGAAGTCCCCGGACGCGAACTGTCCGGTGTCGTCCAGGCCATGGAGTACCTGGAGCACCAGAACCGGCT
CGTCTCGGGCCATGGCCAGAAGGACCCACGCCTGGACGCGGCCGGCCGGCACGTCGTGGTGCTGGGCGGCGGCGACACCG
GCTCGGACTGCCTGGGTACCGCGCTGCGTCAGGGGGCGGCGAGCGTGCGGCAGGTGGAGCTCCTCCCCGCGCCGCCCCCG
GTGCGCGCGGAGGGCAACCCCTGGCCGAGGTGGCCCGTCATCTTCCGCACCTCCACCAGCCAGGAGGAAGGCGGCGAGCG
CCGCTTCGCGCTGATGACGAAGCGGCTGACGGGCAGCGACGGACGGCTCCAGACGCTGCACGCCGTGGAAATCGAGTTCC
AGCCCGGTCCTGGCGCCCTGCCCCGCCTCATTGAACGTCCCGGCACCGAGGTGACGTTCGAAACGGACCTGCTGGTGCTG
GCCATGGGCTTCACGGGGCCCGAGGCGGGCAACCTCTCCGAGGAGTTGGGGGTGAAGCTGTCACCCCGCGGCACGGTGCA
GGTGGACGCGCGCTTCGCCACGTCCGCGGACGGGGTATTCTGCGCCGGGGACGCGAGCCGGGGCGCCAGCCTCATCGTCT
GGGCCCTGTCGGACGGCCGCGAAGCCGCCAAGGCCTGCGATGCGTATCTTTCTGGGGGGCGTTCCGCGCTGCCCACCCGG
GGCACGGACTGCGCCTTCTGA

Upstream 100 bases:

>100_bases
GGACTACAAGCGGGTCCTCCAGGCACGGCGCGCGGCGCGCAGACCACCGGAGCCCACATCCGCGGCGCTGCCCCAGTCCG
CGGCGGGGAGGGCCTGAGCC

Downstream 100 bases:

>100_bases
GCGGAATGAAACGACCGGGTCGCCTTTTGGGGGCGACTCGGGAATAAATAGCAGGCGAGGCAGTTAGAATGGGCTTAACC
CATTACTCCCTCCCACAGGA

Product: glutamate synthase small subunit

Products: NA

Alternate protein names: NADPH-GOGAT [H]

Number of amino acids: Translated: 486; Mature: 485

Protein sequence:

>486_residues
MGKPTGFIEWERVHADKRDKADRLGDWREFALPLAPDEAKRQAGRCMDCGVPFCHQGCPLGNLIPDFNEAVYRGRWREAY
DLLSRTNGFPEMTGRLCPAPCEAACVLAIDRDPVTIEQMEKEIVERAFTEGWVKPRPPARRTGKTVGVVGSGPAGLAAAA
QLNAAGHTVTVYERDARAGGLLRYGIPDFKLEKSVVDRRLALMEAEGITFVTGVDVGGALSYRELRAKHDALVLAMGARR
PRELEVPGRELSGVVQAMEYLEHQNRLVSGHGQKDPRLDAAGRHVVVLGGGDTGSDCLGTALRQGAASVRQVELLPAPPP
VRAEGNPWPRWPVIFRTSTSQEEGGERRFALMTKRLTGSDGRLQTLHAVEIEFQPGPGALPRLIERPGTEVTFETDLLVL
AMGFTGPEAGNLSEELGVKLSPRGTVQVDARFATSADGVFCAGDASRGASLIVWALSDGREAAKACDAYLSGGRSALPTR
GTDCAF

Sequences:

>Translated_486_residues
MGKPTGFIEWERVHADKRDKADRLGDWREFALPLAPDEAKRQAGRCMDCGVPFCHQGCPLGNLIPDFNEAVYRGRWREAY
DLLSRTNGFPEMTGRLCPAPCEAACVLAIDRDPVTIEQMEKEIVERAFTEGWVKPRPPARRTGKTVGVVGSGPAGLAAAA
QLNAAGHTVTVYERDARAGGLLRYGIPDFKLEKSVVDRRLALMEAEGITFVTGVDVGGALSYRELRAKHDALVLAMGARR
PRELEVPGRELSGVVQAMEYLEHQNRLVSGHGQKDPRLDAAGRHVVVLGGGDTGSDCLGTALRQGAASVRQVELLPAPPP
VRAEGNPWPRWPVIFRTSTSQEEGGERRFALMTKRLTGSDGRLQTLHAVEIEFQPGPGALPRLIERPGTEVTFETDLLVL
AMGFTGPEAGNLSEELGVKLSPRGTVQVDARFATSADGVFCAGDASRGASLIVWALSDGREAAKACDAYLSGGRSALPTR
GTDCAF
>Mature_485_residues
GKPTGFIEWERVHADKRDKADRLGDWREFALPLAPDEAKRQAGRCMDCGVPFCHQGCPLGNLIPDFNEAVYRGRWREAYD
LLSRTNGFPEMTGRLCPAPCEAACVLAIDRDPVTIEQMEKEIVERAFTEGWVKPRPPARRTGKTVGVVGSGPAGLAAAAQ
LNAAGHTVTVYERDARAGGLLRYGIPDFKLEKSVVDRRLALMEAEGITFVTGVDVGGALSYRELRAKHDALVLAMGARRP
RELEVPGRELSGVVQAMEYLEHQNRLVSGHGQKDPRLDAAGRHVVVLGGGDTGSDCLGTALRQGAASVRQVELLPAPPPV
RAEGNPWPRWPVIFRTSTSQEEGGERRFALMTKRLTGSDGRLQTLHAVEIEFQPGPGALPRLIERPGTEVTFETDLLVLA
MGFTGPEAGNLSEELGVKLSPRGTVQVDARFATSADGVFCAGDASRGASLIVWALSDGREAAKACDAYLSGGRSALPTRG
TDCAF

Specific function: NITROGEN METABOLISM, GLUTAMATE BIOSYNTHESIS. THE CATALYZED REACTION BRINGS TOGETHER THE NITROGEN AND CARBON METABOLISM. [C]

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=457, Percent_Identity=24.507658643326, Blast_Score=94, Evalue=4e-19,
Organism=Homo sapiens, GI111118981, Length=155, Percent_Identity=34.1935483870968, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI111118983, Length=154, Percent_Identity=34.4155844155844, Blast_Score=72, Evalue=2e-12,
Organism=Escherichia coli, GI1789606, Length=479, Percent_Identity=32.776617954071, Blast_Score=232, Evalue=4e-62,
Organism=Escherichia coli, GI1788811, Length=465, Percent_Identity=34.6236559139785, Blast_Score=231, Evalue=7e-62,
Organism=Escherichia coli, GI87082180, Length=471, Percent_Identity=33.5456475583864, Blast_Score=223, Evalue=2e-59,
Organism=Escherichia coli, GI1788468, Length=439, Percent_Identity=27.3348519362187, Blast_Score=138, Evalue=9e-34,
Organism=Escherichia coli, GI1789243, Length=428, Percent_Identity=30.3738317757009, Blast_Score=118, Evalue=9e-28,
Organism=Caenorhabditis elegans, GI17570289, Length=487, Percent_Identity=45.1745379876797, Blast_Score=427, Evalue=1e-120,
Organism=Caenorhabditis elegans, GI71984108, Length=452, Percent_Identity=25.6637168141593, Blast_Score=89, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17543860, Length=156, Percent_Identity=30.7692307692308, Blast_Score=79, Evalue=6e-15,
Organism=Saccharomyces cerevisiae, GI6320030, Length=492, Percent_Identity=46.9512195121951, Blast_Score=478, Evalue=1e-135,
Organism=Drosophila melanogaster, GI28574881, Length=489, Percent_Identity=47.239263803681, Blast_Score=461, Evalue=1e-130,
Organism=Drosophila melanogaster, GI24665539, Length=489, Percent_Identity=47.239263803681, Blast_Score=461, Evalue=1e-130,
Organism=Drosophila melanogaster, GI24665547, Length=489, Percent_Identity=47.239263803681, Blast_Score=459, Evalue=1e-129,
Organism=Drosophila melanogaster, GI24665543, Length=489, Percent_Identity=47.239263803681, Blast_Score=459, Evalue=1e-129,
Organism=Drosophila melanogaster, GI24640763, Length=469, Percent_Identity=25.5863539445629, Blast_Score=90, Evalue=4e-18,
Organism=Drosophila melanogaster, GI18858217, Length=469, Percent_Identity=25.5863539445629, Blast_Score=90, Evalue=4e-18,
Organism=Drosophila melanogaster, GI17137178, Length=157, Percent_Identity=29.9363057324841, Blast_Score=69, Evalue=7e-12,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR006005
- InterPro:   IPR009051
- InterPro:   IPR016040
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.4.1.13 [H]

Molecular weight: Translated: 52400; Mature: 52269

Theoretical pI: Translated: 6.71; Mature: 6.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKPTGFIEWERVHADKRDKADRLGDWREFALPLAPDEAKRQAGRCMDCGVPFCHQGCPL
CCCCCCCEEEHHHCCCCHHHHHHCCCHHHHCCCCCCCHHHHHHCCEEECCCCHHHCCCCC
GNLIPDFNEAVYRGRWREAYDLLSRTNGFPEMTGRLCPAPCEAACVLAIDRDPVTIEQME
CCCCCCHHHHHHHCCHHHHHHHHHHCCCCHHHHCCCCCCCCCCEEEEEECCCCCCHHHHH
KEIVERAFTEGWVKPRPPARRTGKTVGVVGSGPAGLAAAAQLNAAGHTVTVYERDARAGG
HHHHHHHHHHCCCCCCCCHHHCCCEEEEEECCCCCHHHHHEECCCCCEEEEEECCCCCCC
LLRYGIPDFKLEKSVVDRRLALMEAEGITFVTGVDVGGALSYRELRAKHDALVLAMGARR
EEEECCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHCCCEEEEEECCCC
PRELEVPGRELSGVVQAMEYLEHQNRLVSGHGQKDPRLDAAGRHVVVLGGGDTGSDCLGT
CCCCCCCCHHHHHHHHHHHHHHHHCCEECCCCCCCCCCCCCCCEEEEEECCCCCHHHHHH
ALRQGAASVRQVELLPAPPPVRAEGNPWPRWPVIFRTSTSQEEGGERRFALMTKRLTGSD
HHHHHHHHHEEEEEECCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCEEEEEEHHHCCCC
GRLQTLHAVEIEFQPGPGALPRLIERPGTEVTFETDLLVLAMGFTGPEAGNLSEELGVKL
CCEEEEEEEEEEECCCCCHHHHHHHCCCCEEEEECCEEEEEECCCCCCCCCCHHHHCEEE
SPRGTVQVDARFATSADGVFCAGDASRGASLIVWALSDGREAAKACDAYLSGGRSALPTR
CCCCCEEEEEEEECCCCCEEEECCCCCCCEEEEEEECCCHHHHHHHHHHHCCCCCCCCCC
GTDCAF
CCCCCC
>Mature Secondary Structure 
GKPTGFIEWERVHADKRDKADRLGDWREFALPLAPDEAKRQAGRCMDCGVPFCHQGCPL
CCCCCCEEEHHHCCCCHHHHHHCCCHHHHCCCCCCCHHHHHHCCEEECCCCHHHCCCCC
GNLIPDFNEAVYRGRWREAYDLLSRTNGFPEMTGRLCPAPCEAACVLAIDRDPVTIEQME
CCCCCCHHHHHHHCCHHHHHHHHHHCCCCHHHHCCCCCCCCCCEEEEEECCCCCCHHHHH
KEIVERAFTEGWVKPRPPARRTGKTVGVVGSGPAGLAAAAQLNAAGHTVTVYERDARAGG
HHHHHHHHHHCCCCCCCCHHHCCCEEEEEECCCCCHHHHHEECCCCCEEEEEECCCCCCC
LLRYGIPDFKLEKSVVDRRLALMEAEGITFVTGVDVGGALSYRELRAKHDALVLAMGARR
EEEECCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHCCCEEEEEECCCC
PRELEVPGRELSGVVQAMEYLEHQNRLVSGHGQKDPRLDAAGRHVVVLGGGDTGSDCLGT
CCCCCCCCHHHHHHHHHHHHHHHHCCEECCCCCCCCCCCCCCCEEEEEECCCCCHHHHHH
ALRQGAASVRQVELLPAPPPVRAEGNPWPRWPVIFRTSTSQEEGGERRFALMTKRLTGSD
HHHHHHHHHEEEEEECCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCEEEEEEHHHCCCC
GRLQTLHAVEIEFQPGPGALPRLIERPGTEVTFETDLLVLAMGFTGPEAGNLSEELGVKL
CCEEEEEEEEEEECCCCCHHHHHHHCCCCEEEEECCEEEEEECCCCCCCCCCHHHHCEEE
SPRGTVQVDARFATSADGVFCAGDASRGASLIVWALSDGREAAKACDAYLSGGRSALPTR
CCCCCEEEEEEEECCCCCEEEECCCCCCCEEEEEEECCCHHHHHHHHHHHCCCCCCCCCC
GTDCAF
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377; 11029411 [H]