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The map label for this gene is thiF [C]

Identifier: 108760863

GI number: 108760863

Start: 4985283

End: 4985924

Strand: Reverse

Name: thiF [C]

Synonym: MXAN_4066

Alternate gene names: 108760863

Gene position: 4985924-4985283 (Counterclockwise)

Preceding gene: 108761704

Following gene: 108762420

Centisome position: 54.55

GC content: 76.01

Gene sequence:

>642_bases
ATGGCGCTGCGCGAAGATCAGATCCTGCGCTACTCCCGGCAGATCCTCCTGCGCGAGGTGGGCGGACGTGGCCAGGAGTC
CCTGCTCGCGGGCACGGCGCGGTTGGATGGCATTGGCGCGTCGGGGCTGACGGCGGCGGCGTACCTGGCGGGAGGTGGCA
CGCCGGTGACGGGCGCGGGCTCGCTGACGCTGGGGCCCTGGGCGCCGGGCTTCCTGATGGGGCCTTCCGACGTGGGCCTG
CCCGTGACGGAGGCGCTGGCCCGCGAGGTGCCCGCGCTGAATCCGGACGCGGGCGCGGTGGGGCAGGGGGGCGGGCTGGT
GGCGGAGCTGCCCGCGGCGTGGAGTGGCGAGGCGCCCTGGGTGGCGCTGGGGGGCGACGGCATGCGGGCCGCCGTTGTCT
TCCGAGGCCCCGAGGGGTGCCTCTGGTGCTTCGGCGAGACGGTGCGGCACCTGGGCTCGCCCCCGGATGGGGCGCTGGGC
GTGGCCGTGGGGACGCTGGGAGCGCTCGTGTTTCAGCGGCTGCGGTTGGGCCTGGGCCCCGCGCTGGGCGGCCGGTGGTT
GGTGGCACCTGGAACCGTGGAGGAGATGGAAATGCGCAGGTGCTCGCGCTGCGCGGGCCGTGAGCCGCCCGCCGAGTCGT
GA

Upstream 100 bases:

>100_bases
TCAACGACGAGGACATCCGCGCGCTGAACGGGTTGGACACGCCGGTGAAGGACGCTGACCGCATCACCCTCATCCCCGCC
ATGGCGGGGGGCTGACGGCC

Downstream 100 bases:

>100_bases
GTCACTGGCCCGAGCACATCCTCGACGAGGTGGGACGTCACCTGGAGGCCGCGTATCCGTTCGAGGGCTGTGGGGTGATC
CTCCGCGCGGAGGGGTCGGG

Product: hypothetical protein

Products: ThiS-COSH; AMP; ThiS-COAMP; pyrophosphate [C]

Alternate protein names: None

Number of amino acids: Translated: 213; Mature: 212

Protein sequence:

>213_residues
MALREDQILRYSRQILLREVGGRGQESLLAGTARLDGIGASGLTAAAYLAGGGTPVTGAGSLTLGPWAPGFLMGPSDVGL
PVTEALAREVPALNPDAGAVGQGGGLVAELPAAWSGEAPWVALGGDGMRAAVVFRGPEGCLWCFGETVRHLGSPPDGALG
VAVGTLGALVFQRLRLGLGPALGGRWLVAPGTVEEMEMRRCSRCAGREPPAES

Sequences:

>Translated_213_residues
MALREDQILRYSRQILLREVGGRGQESLLAGTARLDGIGASGLTAAAYLAGGGTPVTGAGSLTLGPWAPGFLMGPSDVGL
PVTEALAREVPALNPDAGAVGQGGGLVAELPAAWSGEAPWVALGGDGMRAAVVFRGPEGCLWCFGETVRHLGSPPDGALG
VAVGTLGALVFQRLRLGLGPALGGRWLVAPGTVEEMEMRRCSRCAGREPPAES
>Mature_212_residues
ALREDQILRYSRQILLREVGGRGQESLLAGTARLDGIGASGLTAAAYLAGGGTPVTGAGSLTLGPWAPGFLMGPSDVGLP
VTEALAREVPALNPDAGAVGQGGGLVAELPAAWSGEAPWVALGGDGMRAAVVFRGPEGCLWCFGETVRHLGSPPDGALGV
AVGTLGALVFQRLRLGLGPALGGRWLVAPGTVEEMEMRRCSRCAGREPPAES

Specific function: Catalyzes The Adenylation By ATP Of The Carboxyl- Terminal Glycine Of This. [C]

COG id: COG0476

COG function: function code H; Dinucleotide-utilizing enzymes involved in molybdopterin and thiamine biosynthesis family 2

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 2.7.7.- [C]

Molecular weight: Translated: 21606; Mature: 21475

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: PS00213 LIPOCALIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALREDQILRYSRQILLREVGGRGQESLLAGTARLDGIGASGLTAAAYLAGGGTPVTGAG
CCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCC
SLTLGPWAPGFLMGPSDVGLPVTEALAREVPALNPDAGAVGQGGGLVAELPAAWSGEAPW
CEEECCCCCCCEECCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCCCCCCCCE
VALGGDGMRAAVVFRGPEGCLWCFGETVRHLGSPPDGALGVAVGTLGALVFQRLRLGLGP
EEECCCCCEEEEEEECCCCEEEHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCC
ALGGRWLVAPGTVEEMEMRRCSRCAGREPPAES
CCCCEEEECCCCHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
ALREDQILRYSRQILLREVGGRGQESLLAGTARLDGIGASGLTAAAYLAGGGTPVTGAG
CCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCC
SLTLGPWAPGFLMGPSDVGLPVTEALAREVPALNPDAGAVGQGGGLVAELPAAWSGEAPW
CEEECCCCCCCEECCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCCCCCCCCE
VALGGDGMRAAVVFRGPEGCLWCFGETVRHLGSPPDGALGVAVGTLGALVFQRLRLGLGP
EEECCCCCEEEEEEECCCCEEEHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCC
ALGGRWLVAPGTVEEMEMRRCSRCAGREPPAES
CCCCEEEECCCCHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ThiS-COAMP; L-cysteine; ThiS protein; ATP [C]

Specific reaction: ThiS-COAMP + L-cysteine = ThiS-COSH + AMP ThiS protein + ATP = ThiS-COAMP + pyrophosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA