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The map label for this gene is spo0F [H]

Identifier: 108760778

GI number: 108760778

Start: 3470329

End: 3470706

Strand: Direct

Name: spo0F [H]

Synonym: MXAN_2962

Alternate gene names: 108760778

Gene position: 3470329-3470706 (Clockwise)

Preceding gene: 108757205

Following gene: 108760089

Centisome position: 37.97

GC content: 65.34

Gene sequence:

>378_bases
GTGGTCCTGGTCGTCGATGACGACCCGGACATCCTGGAGGCCCTTTCAGAGATTCTGGAAGCCGAAGGCTTCGAAATCCG
CCGGGCGCGGAACGGGAAGGAGGCGTTGGACCGGCTGGAGCCCGAACCGCCCAACCTCATCCTGTTGGACCTGATGATGC
CGGTGATGGACGGTTGGGAGTTCGCCCAGCGGATGCGGCAGAAGCCGCCCGAGGTGGCGCGCATCCCCCTCATCGTCCTG
AGCGCGGACCGCAATGTGGGCAGCAAGGCCGCAGACATTGGCGCGGTGGGGCATCTGGCCAAGCCGTTCGAACTCAACGA
TTTGTTGGACATGGTTCGCCGCTCGCTGAACCCCGCCGCCGCGTCCACCATCGCGTGA

Upstream 100 bases:

>100_bases
TTTCTTGCGCGGCCGTGTGGGAGGATGGTTGAAGAATCCCAGCGTCGCACCTCGGGAGACTGCTCTGACCTTGCACAATG
CACAGCATGCGAGAGCCCCG

Downstream 100 bases:

>100_bases
CAGTTGCCGCATCCGTGTCTTGACCTGCCAGGGGGGCGCCATTACGGTCCCCCCTGTCTTTGATTTTCAAATCAACGATC
AGGCCAAGGAGTGTGTCGTG

Product: response regulator

Products: NA

Alternate protein names: Stage 0 sporulation protein F [H]

Number of amino acids: Translated: 125; Mature: 125

Protein sequence:

>125_residues
MVLVVDDDPDILEALSEILEAEGFEIRRARNGKEALDRLEPEPPNLILLDLMMPVMDGWEFAQRMRQKPPEVARIPLIVL
SADRNVGSKAADIGAVGHLAKPFELNDLLDMVRRSLNPAAASTIA

Sequences:

>Translated_125_residues
MVLVVDDDPDILEALSEILEAEGFEIRRARNGKEALDRLEPEPPNLILLDLMMPVMDGWEFAQRMRQKPPEVARIPLIVL
SADRNVGSKAADIGAVGHLAKPFELNDLLDMVRRSLNPAAASTIA
>Mature_125_residues
MVLVVDDDPDILEALSEILEAEGFEIRRARNGKEALDRLEPEPPNLILLDLMMPVMDGWEFAQRMRQKPPEVARIPLIVL
SADRNVGSKAADIGAVGHLAKPFELNDLLDMVRRSLNPAAASTIA

Specific function: Key element in the phosphorelay regulating sporulation initiation. Phosphorylation of spo0B during sporulation initiation [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=118, Percent_Identity=33.8983050847458, Blast_Score=68, Evalue=2e-13,
Organism=Escherichia coli, GI1790299, Length=119, Percent_Identity=31.9327731092437, Blast_Score=65, Evalue=1e-12,
Organism=Escherichia coli, GI87082012, Length=122, Percent_Identity=31.1475409836066, Blast_Score=61, Evalue=2e-11,
Organism=Escherichia coli, GI1786911, Length=124, Percent_Identity=35.4838709677419, Blast_Score=60, Evalue=4e-11,
Organism=Escherichia coli, GI1790552, Length=114, Percent_Identity=33.3333333333333, Blast_Score=59, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 13795; Mature: 13795

Theoretical pI: Translated: 4.35; Mature: 4.35

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVLVVDDDPDILEALSEILEAEGFEIRRARNGKEALDRLEPEPPNLILLDLMMPVMDGWE
CEEEECCCHHHHHHHHHHHHCCCCHHHHHCCHHHHHHHCCCCCCCEEHHHHHHHHHCCHH
FAQRMRQKPPEVARIPLIVLSADRNVGSKAADIGAVGHLAKPFELNDLLDMVRRSLNPAA
HHHHHHCCCCCCHHCCEEEEECCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHH
ASTIA
HHCCC
>Mature Secondary Structure
MVLVVDDDPDILEALSEILEAEGFEIRRARNGKEALDRLEPEPPNLILLDLMMPVMDGWE
CEEEECCCHHHHHHHHHHHHCCCCHHHHHCCHHHHHHHCCCCCCCEEHHHHHHHHHCCHH
FAQRMRQKPPEVARIPLIVLSADRNVGSKAADIGAVGHLAKPFELNDLLDMVRRSLNPAA
HHHHHHCCCCCCHHCCEEEEECCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHH
ASTIA
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3003689; 2997779; 2457578; 9353933; 9384377; 8528078; 9254596; 8805550; 9335530; 10997904 [H]