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The map label for this gene is metF [H]

Identifier: 108760527

GI number: 108760527

Start: 3565349

End: 3566221

Strand: Reverse

Name: metF [H]

Synonym: MXAN_3039

Alternate gene names: 108760527

Gene position: 3566221-3565349 (Counterclockwise)

Preceding gene: 108758191

Following gene: 108757147

Centisome position: 39.02

GC content: 65.52

Gene sequence:

>873_bases
ATGAAGATTCGTAATCGGTTGAATCCTTCCGACCCGTGCTTCTCCTTCGAGTTCTTCCCGCCCCGGACGGACGAGGGCGA
GGCGAACCTGCTCAAGGCGCTGGAAGACCTGGCGACCCTGCAGCCGGGTTTCGTCTCCGTGACGGAAGGCGCGGGAGGCA
GCACTCGGGCGAAGACGGTGGAGTTGGTGCTGCGCATCAAGCAGGAGGCGGGTATCGAGGCGATGGCGCACCTGACATGC
GGGGGGCACTCCCCGGCGGAGCTGCGCACCGTGCTGGAGCGCCTGTCGGCCGCGAAGGTGGACAACGTCCTGGTGCTGCG
CGGAGACCCACCCAAGGGCCAGACGGTCTTCGAGCCCGCGCCCGGGGGTTTCCGCTACGCGTCGGAGATGGTGCGATTCA
TCCGAGAAGAGGATTTCAACTTCTGCCTCGGGGGAGCGTGCTATCCGGAGGGCCACGTGGAGACGCCCTCCCGTGACGAC
GACCTGCGTCATCTCAAGGCCAAGGTGGATGCCGGCCTGGACTTCGTGGTGACGCAGCTCTTCTTCGACAACGCGTTCTA
CTTCGACTTCGTGGAGCGGGCGCGCCGCGCGGGCATCAACGTCCCCATCGTCCCCGGCATCATGCCCATCACCAACTACG
AGCAGATTCAACGATTCACGCGCCTGTGCGGAGCCACCGTGCCCATGCGCCTGGCGTTGCAGCTCGAGCGGGTGAAGGAC
AACCCGGAGGCCATGGCCCAGCTCGGCGTGGCCCATGCGACGGTGCAGTGCATGGAGTTGCTGTCGCGCGGCGTGCCTGG
CATCCACTTCTACACGCTCAACAAGTCCCCGGCGACGCGGATGATCGTGAGCGCACTGAGAGCCCGTTCATGA

Upstream 100 bases:

>100_bases
CCATCTTCATTCTCCTGGAGGGGTGTGGAAAACGGCGCGGACTATAGCCGCGAGACTTGCCCGATCAAGGACGACGCGGA
GCGTCTACACTGGGGGCGAC

Downstream 100 bases:

>100_bases
GTGGTCCTGGAATCCACCTGCCCCCAAGCGACCCGCGTAGCAAGGTCCTCAACATCATCCGTCCGCCCGCCTTCTTCCTG
CTGTGCGTGGGCGTGCTGAA

Product: 5,10-methylenetetrahydrofolate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 290; Mature: 290

Protein sequence:

>290_residues
MKIRNRLNPSDPCFSFEFFPPRTDEGEANLLKALEDLATLQPGFVSVTEGAGGSTRAKTVELVLRIKQEAGIEAMAHLTC
GGHSPAELRTVLERLSAAKVDNVLVLRGDPPKGQTVFEPAPGGFRYASEMVRFIREEDFNFCLGGACYPEGHVETPSRDD
DLRHLKAKVDAGLDFVVTQLFFDNAFYFDFVERARRAGINVPIVPGIMPITNYEQIQRFTRLCGATVPMRLALQLERVKD
NPEAMAQLGVAHATVQCMELLSRGVPGIHFYTLNKSPATRMIVSALRARS

Sequences:

>Translated_290_residues
MKIRNRLNPSDPCFSFEFFPPRTDEGEANLLKALEDLATLQPGFVSVTEGAGGSTRAKTVELVLRIKQEAGIEAMAHLTC
GGHSPAELRTVLERLSAAKVDNVLVLRGDPPKGQTVFEPAPGGFRYASEMVRFIREEDFNFCLGGACYPEGHVETPSRDD
DLRHLKAKVDAGLDFVVTQLFFDNAFYFDFVERARRAGINVPIVPGIMPITNYEQIQRFTRLCGATVPMRLALQLERVKD
NPEAMAQLGVAHATVQCMELLSRGVPGIHFYTLNKSPATRMIVSALRARS
>Mature_290_residues
MKIRNRLNPSDPCFSFEFFPPRTDEGEANLLKALEDLATLQPGFVSVTEGAGGSTRAKTVELVLRIKQEAGIEAMAHLTC
GGHSPAELRTVLERLSAAKVDNVLVLRGDPPKGQTVFEPAPGGFRYASEMVRFIREEDFNFCLGGACYPEGHVETPSRDD
DLRHLKAKVDAGLDFVVTQLFFDNAFYFDFVERARRAGINVPIVPGIMPITNYEQIQRFTRLCGATVPMRLALQLERVKD
NPEAMAQLGVAHATVQCMELLSRGVPGIHFYTLNKSPATRMIVSALRARS

Specific function: Methionine biosynthesis. [C]

COG id: COG0685

COG function: function code E; 5,10-methylenetetrahydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methylenetetrahydrofolate reductase family [H]

Homologues:

Organism=Homo sapiens, GI87240000, Length=293, Percent_Identity=39.2491467576792, Blast_Score=203, Evalue=1e-52,
Organism=Escherichia coli, GI1790377, Length=277, Percent_Identity=38.9891696750903, Blast_Score=190, Evalue=1e-49,
Organism=Caenorhabditis elegans, GI25147441, Length=292, Percent_Identity=36.3013698630137, Blast_Score=177, Evalue=7e-45,
Organism=Caenorhabditis elegans, GI25147436, Length=292, Percent_Identity=36.3013698630137, Blast_Score=177, Evalue=8e-45,
Organism=Saccharomyces cerevisiae, GI6325234, Length=299, Percent_Identity=36.1204013377926, Blast_Score=177, Evalue=1e-45,
Organism=Saccharomyces cerevisiae, GI6321313, Length=291, Percent_Identity=33.6769759450172, Blast_Score=176, Evalue=4e-45,
Organism=Drosophila melanogaster, GI24662658, Length=213, Percent_Identity=28.169014084507, Blast_Score=77, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003171
- InterPro:   IPR004620 [H]

Pfam domain/function: PF02219 MTHFR [H]

EC number: =1.5.1.20 [H]

Molecular weight: Translated: 32068; Mature: 32068

Theoretical pI: Translated: 7.09; Mature: 7.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIRNRLNPSDPCFSFEFFPPRTDEGEANLLKALEDLATLQPGFVSVTEGAGGSTRAKTV
CCCCCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHH
ELVLRIKQEAGIEAMAHLTCGGHSPAELRTVLERLSAAKVDNVLVLRGDPPKGQTVFEPA
HHHHHHHHHCCHHHHHHEECCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCCEEECCC
PGGFRYASEMVRFIREEDFNFCLGGACYPEGHVETPSRDDDLRHLKAKVDAGLDFVVTQL
CCHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHH
FFDNAFYFDFVERARRAGINVPIVPGIMPITNYEQIQRFTRLCGATVPMRLALQLERVKD
HHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHCC
NPEAMAQLGVAHATVQCMELLSRGVPGIHFYTLNKSPATRMIVSALRARS
CHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MKIRNRLNPSDPCFSFEFFPPRTDEGEANLLKALEDLATLQPGFVSVTEGAGGSTRAKTV
CCCCCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHH
ELVLRIKQEAGIEAMAHLTCGGHSPAELRTVLERLSAAKVDNVLVLRGDPPKGQTVFEPA
HHHHHHHHHCCHHHHHHEECCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCCEEECCC
PGGFRYASEMVRFIREEDFNFCLGGACYPEGHVETPSRDDDLRHLKAKVDAGLDFVVTQL
CCHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHH
FFDNAFYFDFVERARRAGINVPIVPGIMPITNYEQIQRFTRLCGATVPMRLALQLERVKD
HHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHCC
NPEAMAQLGVAHATVQCMELLSRGVPGIHFYTLNKSPATRMIVSALRARS
CHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]