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The map label for this gene is pfkA [H]

Identifier: 108760502

GI number: 108760502

Start: 4919928

End: 4921013

Strand: Direct

Name: pfkA [H]

Synonym: MXAN_4016

Alternate gene names: 108760502

Gene position: 4919928-4921013 (Clockwise)

Preceding gene: 108759386

Following gene: 108756816

Centisome position: 53.83

GC content: 69.61

Gene sequence:

>1086_bases
ATGAGACTTGGAGTCCTGACCGGCGGTGGCGACTGCCCCGGCCTCAACGCACTCATTCGCGGCCTCGTGAAGCGAGGCAC
GCACGAATTCGGCTACGAGTTCGTGGGCATCGAGAACGGCTACATGGGGCTGGTGGAGCCAGGGCTCGCCCACCCGCTCA
CCGAGGAGGACACCCGCGGCATCCTCCCCAAGGGCGGCACCATCCTGGGCACGTCCAACAAGGCCAACCCCTTCAGCTAC
GCGACCCGTGAGGATGGGCACTGGGTGGAGCGGGACGTGTCCGATCAGGTCCTGCTCCGCTGCGAGGAGCTGGGCCTGGA
CGGGCTCATCGCCGTGGGCGGCGACGGGACGCTGTCCATCGCCCACCGGCTCGTGGAGAAGGGGCTCAAGGTCGTCGGCT
GTCCGAAGACCATCGACAACGACCTGTCCGGGACGGATCAGACCTTCGGCTTCGACACCGCGCGGCTCATCGTCACCGAG
GCGCTCGACCGCCTGCACTCCACCGCCGAGGCCCACGACCGGGTGATGGTCGTGGAGATCATGGGCCGCCACGCCGGCTT
CCTCACCCTGGAGAGCGGCATCGCCGGGGGCGCGGACGTCATCCTGATTCCGGAGATTCCGTACAGCGTGGAGTCCGTGG
TGGAGAAGATCCGCCGCCGCTCCACCCGCCGTCGCAGCTTCTCCATCATCGCCATCTCCGAAGGCGCGTTCCCCCAGGGC
GGCGAGCTGGCCGTGCTGGACACGGCGGAGGCCATTCCCGGCCGGGGCGTGGTGCGGCTCGGCGGCTCGGGGAAGGCGCT
GGCGGACCTGCTGGCCCGGCACATCGAGGCGGAGATTCGCGTGACGGTGCTGGGCCACCTCCAGCGCGGGGGCAGCCCCA
GCGCGGCGGACCGGGTACTGGCCACTCGCTACGGCTGCAAGGTGCTGGACCTGGTGAGCGCGGGCCAGTGGGACCACATG
GTGGCCCTGCGTGCGGGCGAAATCATCGCGGTCCCCTTGAGCGAGTCGCGCAAGGAGCGCCGCGTGGATCCGGCGGGTGA
GCTGGTGCGCTTCACCAAGAGCATGGGCATCAGCTTCGGGGACTGA

Upstream 100 bases:

>100_bases
TGCACCCCCGGGGGGCCCCCCCTACAATCAGGTCCGACGTTTCTGTCATGGTGAAGCACCCTCCCTCTGACACCCGCGTC
CGACCTGTATGCCCCGTCCC

Downstream 100 bases:

>100_bases
GGGGACCGCCAAACCGCCATGACGACGCTCGTCGGCCGCCATATCGGTCGCTACCGCATCCTCGAGCAACTGGGCTCGGG
GGGCATGAGCGTCGTGTACA

Product: 6-phosphofructokinase I

Products: NA

Alternate protein names: Phosphofructokinase 2; Phosphohexokinase 2 [H]

Number of amino acids: Translated: 361; Mature: 361

Protein sequence:

>361_residues
MRLGVLTGGGDCPGLNALIRGLVKRGTHEFGYEFVGIENGYMGLVEPGLAHPLTEEDTRGILPKGGTILGTSNKANPFSY
ATREDGHWVERDVSDQVLLRCEELGLDGLIAVGGDGTLSIAHRLVEKGLKVVGCPKTIDNDLSGTDQTFGFDTARLIVTE
ALDRLHSTAEAHDRVMVVEIMGRHAGFLTLESGIAGGADVILIPEIPYSVESVVEKIRRRSTRRRSFSIIAISEGAFPQG
GELAVLDTAEAIPGRGVVRLGGSGKALADLLARHIEAEIRVTVLGHLQRGGSPSAADRVLATRYGCKVLDLVSAGQWDHM
VALRAGEIIAVPLSESRKERRVDPAGELVRFTKSMGISFGD

Sequences:

>Translated_361_residues
MRLGVLTGGGDCPGLNALIRGLVKRGTHEFGYEFVGIENGYMGLVEPGLAHPLTEEDTRGILPKGGTILGTSNKANPFSY
ATREDGHWVERDVSDQVLLRCEELGLDGLIAVGGDGTLSIAHRLVEKGLKVVGCPKTIDNDLSGTDQTFGFDTARLIVTE
ALDRLHSTAEAHDRVMVVEIMGRHAGFLTLESGIAGGADVILIPEIPYSVESVVEKIRRRSTRRRSFSIIAISEGAFPQG
GELAVLDTAEAIPGRGVVRLGGSGKALADLLARHIEAEIRVTVLGHLQRGGSPSAADRVLATRYGCKVLDLVSAGQWDHM
VALRAGEIIAVPLSESRKERRVDPAGELVRFTKSMGISFGD
>Mature_361_residues
MRLGVLTGGGDCPGLNALIRGLVKRGTHEFGYEFVGIENGYMGLVEPGLAHPLTEEDTRGILPKGGTILGTSNKANPFSY
ATREDGHWVERDVSDQVLLRCEELGLDGLIAVGGDGTLSIAHRLVEKGLKVVGCPKTIDNDLSGTDQTFGFDTARLIVTE
ALDRLHSTAEAHDRVMVVEIMGRHAGFLTLESGIAGGADVILIPEIPYSVESVVEKIRRRSTRRRSFSIIAISEGAFPQG
GELAVLDTAEAIPGRGVVRLGGSGKALADLLARHIEAEIRVTVLGHLQRGGSPSAADRVLATRYGCKVLDLVSAGQWDHM
VALRAGEIIAVPLSESRKERRVDPAGELVRFTKSMGISFGD

Specific function: Key control step of glycolysis. [C]

COG id: COG0205

COG function: function code G; 6-phosphofructokinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphofructokinase family [H]

Homologues:

Organism=Homo sapiens, GI11321601, Length=363, Percent_Identity=33.3333333333333, Blast_Score=155, Evalue=6e-38,
Organism=Homo sapiens, GI48762920, Length=386, Percent_Identity=31.8652849740933, Blast_Score=153, Evalue=3e-37,
Organism=Homo sapiens, GI266453768, Length=363, Percent_Identity=32.2314049586777, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI266453748, Length=363, Percent_Identity=32.2314049586777, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI4505749, Length=363, Percent_Identity=32.2314049586777, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI266453619, Length=363, Percent_Identity=32.2314049586777, Blast_Score=149, Evalue=3e-36,
Organism=Escherichia coli, GI1790350, Length=328, Percent_Identity=36.280487804878, Blast_Score=198, Evalue=4e-52,
Organism=Caenorhabditis elegans, GI25147584, Length=334, Percent_Identity=28.7425149700599, Blast_Score=135, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI17558788, Length=353, Percent_Identity=31.1614730878187, Blast_Score=123, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI72003601, Length=165, Percent_Identity=32.1212121212121, Blast_Score=91, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6321679, Length=392, Percent_Identity=33.6734693877551, Blast_Score=163, Evalue=4e-41,
Organism=Saccharomyces cerevisiae, GI6323861, Length=388, Percent_Identity=31.9587628865979, Blast_Score=150, Evalue=4e-37,
Organism=Drosophila melanogaster, GI28573326, Length=363, Percent_Identity=31.404958677686, Blast_Score=151, Evalue=8e-37,
Organism=Drosophila melanogaster, GI24652337, Length=364, Percent_Identity=31.3186813186813, Blast_Score=149, Evalue=2e-36,
Organism=Drosophila melanogaster, GI17647809, Length=364, Percent_Identity=31.3186813186813, Blast_Score=149, Evalue=3e-36,

Paralogues:

None

Copy number: 3981 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 950 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012003
- InterPro:   IPR012829
- InterPro:   IPR022953
- InterPro:   IPR015912
- InterPro:   IPR000023 [H]

Pfam domain/function: PF00365 PFK [H]

EC number: =2.7.1.11 [H]

Molecular weight: Translated: 38533; Mature: 38533

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: PS00433 PHOSPHOFRUCTOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLGVLTGGGDCPGLNALIRGLVKRGTHEFGYEFVGIENGYMGLVEPGLAHPLTEEDTRG
CEEEEEECCCCCCCHHHHHHHHHHHCCHHHCEEEEEECCCCEECCCCCCCCCCCCCCCCC
ILPKGGTILGTSNKANPFSYATREDGHWVERDVSDQVLLRCEELGLDGLIAVGGDGTLSI
CCCCCCEEEECCCCCCCCCCCCCCCCCCHHCCCCHHHHHHHHHCCCCEEEEECCCCHHHH
AHRLVEKGLKVVGCPKTIDNDLSGTDQTFGFDTARLIVTEALDRLHSTAEAHDRVMVVEI
HHHHHHCCCEEEECCCHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEEE
MGRHAGFLTLESGIAGGADVILIPEIPYSVESVVEKIRRRSTRRRSFSIIAISEGAFPQG
CCCCCCEEEEECCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHEEEEEEEECCCCCCC
GELAVLDTAEAIPGRGVVRLGGSGKALADLLARHIEAEIRVTVLGHLQRGGSPSAADRVL
CCEEEEECCCCCCCCCEEEECCCCHHHHHHHHHHHCCEEEEEEEEEHHCCCCCHHHHHHH
ATRYGCKVLDLVSAGQWDHMVALRAGEIIAVPLSESRKERRVDPAGELVRFTKSMGISFG
HHHHCCHHEEHHCCCCCCEEEEEECCCEEEECCCCCHHHHCCCCHHHHHHHHHHCCCCCC
D
C
>Mature Secondary Structure
MRLGVLTGGGDCPGLNALIRGLVKRGTHEFGYEFVGIENGYMGLVEPGLAHPLTEEDTRG
CEEEEEECCCCCCCHHHHHHHHHHHCCHHHCEEEEEECCCCEECCCCCCCCCCCCCCCCC
ILPKGGTILGTSNKANPFSYATREDGHWVERDVSDQVLLRCEELGLDGLIAVGGDGTLSI
CCCCCCEEEECCCCCCCCCCCCCCCCCCHHCCCCHHHHHHHHHCCCCEEEEECCCCHHHH
AHRLVEKGLKVVGCPKTIDNDLSGTDQTFGFDTARLIVTEALDRLHSTAEAHDRVMVVEI
HHHHHHCCCEEEECCCHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEEE
MGRHAGFLTLESGIAGGADVILIPEIPYSVESVVEKIRRRSTRRRSFSIIAISEGAFPQG
CCCCCCEEEEECCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHEEEEEEEECCCCCCC
GELAVLDTAEAIPGRGVVRLGGSGKALADLLARHIEAEIRVTVLGHLQRGGSPSAADRVL
CCEEEEECCCCCCCCCEEEECCCCHHHHHHHHHHHCCEEEEEEEEEHHCCCCCHHHHHHH
ATRYGCKVLDLVSAGQWDHMVALRAGEIIAVPLSESRKERRVDPAGELVRFTKSMGISFG
HHHHCCHHEEHHCCCCCCEEEEEECCCEEEECCCCCHHHHCCCCHHHHHHHHHHCCCCCC
D
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11792842 [H]