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The map label for this gene is gadA [C]

Identifier: 108759811

GI number: 108759811

Start: 3363088

End: 3364617

Strand: Direct

Name: gadA [C]

Synonym: MXAN_2880

Alternate gene names: 108759811

Gene position: 3363088-3364617 (Clockwise)

Preceding gene: 108762417

Following gene: 108757953

Centisome position: 36.8

GC content: 68.5

Gene sequence:

>1530_bases
ATGGCACTTCCAGACCTGAAGGCGCTGAGCCTGCTCAACCACGTCCCGCCGAGGCTGCTATCCGCGGCGGAGCGCTACCT
CAAGGCTGTTCCGGGTGTGAAGGGCCTGCTCAACCGGGAGACGGGCTCGCTGCTGTCCGAACTGGAGAGCGGCCTCAAGC
CCTACCGCGGGAAGATGCAGTCGTTCGACCACCTGCCGCCGACGGGGCGCTCGCGCGAGGACGTGCTGCGTGAGCTCCAG
GCCCTGGAGTCGCAGGAGGAGTACCGCTGGCGGGAGGGCCGCGTGTCCGGAGGCGTGTACAACGGCGACGCGGAGCACAT
CGCGTTCCTCAACCGCGTCTATGCGCTGCATTCGCAGAGCAACCCGCTGCACGCCGACCTGTGGCCCAGCGCCACCAAGT
TCGAGGCGGAGGTGGTGGCGATGACGGCCAGCATGCTCGGCGCGGACGTGGCGAACGCGGGCCAGCCGGAGGAGGCGCGC
ATCTGCGGGGCCATGTCGTCCGGTGGCACCGAGAGCATCATGCTGGCGATGAAGACGTACCGGGACTGGGCCCGGGAGAC
CAAGGGCATCACCAGGCCGGAGATGGTGGCGCCCGCCAGCGCGCACCCGGCCTTCGACAAGGCGGCGCACTACTTCGGCA
TCAAGATGGTGCGGGTGCCGGTGGGCCCGGACTACCGTGCGGACGTGGCCGCCATGCGCAAGGCCATCAACCGCAACACC
ATCGTCCTCATCGGCTCGGCGCCCGGCTTTCCGCACGGTGTCATCGACCCCATCGAGGCGCTGTCCGAGATCGCGCGCAA
GAAGCGCATCGGCTTCCACACCGACGCGTGCCTGGGCGGCTTCGTGCTGCCCTTCGCCATGAAGCTGGGCTACGACGTGC
CGCCCTTCGACTTCCGGCTGCCGGGCGTGACGTCCATGTCCGTGGACACGCACAAGTTCGGCTATGCGGCCAAGGGCTCC
TCGGTGGTGTTGTACCGGGGCACGGAGCTGCGCTCGCACCAGTACTTCACGGCGACGGACTGGCCGGGTGGCATCTACTT
CTCGCCCACCTTCTCCGGCAGCCGGCCCGGGGCGCTCATCGCCTCGGCGTGGGCCGCGCTGGTGAGCACGGGGGAGCAGG
GCTACCTGGACGCCACCCGGAGCATCCTGGAGACGGCGGCGGCCATCAAGAAGGGCATCCGGGACATCCCGGAGCTGCAC
GTGCTGGGGGACCCGCTGTTCGTCATCGCCTTCGGTTCGGAGTCCGTGGACATCTTCCAGGTGATGGAGCGGATGAGCGA
GCACGGGTGGAACCTCAACGGCCTGCACAAACCGTCCGCCGTCCACCTGTGCGTCACGCTGCGCCACACGCAGCCCGGCG
TCGCGGAGCAGTTCCTGGTCGACCTGCGCGACGCCGTGGACTACGTGAAGTCCCACCCCAGCGCGAAGGGGACGATGGCG
CCCGTGTACGGCATGGCGGCCTCCGTGCCCTTCCGCGGTCTGGTGAGCGACCTGCTCAAGAAGTACATGGACCTGCTCTA
CAAGGTGTAA

Upstream 100 bases:

>100_bases
ACGAGCTGTTTCGCGAGTTCGTCAACATCTACAAACACAACAAGGCCATCTTCGCGCGGCTCAATCGAGCGCGAAGCGCC
TGAACCCCCAGGAGTTCGCC

Downstream 100 bases:

>100_bases
CGGCGCGGGTCCTACAGCGACCGGCTGGCGATTTCCGCCAGCTCTGCGTCGGTGAGCACCAGGGGGTTGGCCTTCATGCT
GCTGGCCTCCTTGGCCTTCG

Product: putative sphingosine-1-phosphate lyase

Products: NA

Alternate protein names: SP-lyase; SPL; Sphingosine-1-phosphate aldolase [H]

Number of amino acids: Translated: 509; Mature: 508

Protein sequence:

>509_residues
MALPDLKALSLLNHVPPRLLSAAERYLKAVPGVKGLLNRETGSLLSELESGLKPYRGKMQSFDHLPPTGRSREDVLRELQ
ALESQEEYRWREGRVSGGVYNGDAEHIAFLNRVYALHSQSNPLHADLWPSATKFEAEVVAMTASMLGADVANAGQPEEAR
ICGAMSSGGTESIMLAMKTYRDWARETKGITRPEMVAPASAHPAFDKAAHYFGIKMVRVPVGPDYRADVAAMRKAINRNT
IVLIGSAPGFPHGVIDPIEALSEIARKKRIGFHTDACLGGFVLPFAMKLGYDVPPFDFRLPGVTSMSVDTHKFGYAAKGS
SVVLYRGTELRSHQYFTATDWPGGIYFSPTFSGSRPGALIASAWAALVSTGEQGYLDATRSILETAAAIKKGIRDIPELH
VLGDPLFVIAFGSESVDIFQVMERMSEHGWNLNGLHKPSAVHLCVTLRHTQPGVAEQFLVDLRDAVDYVKSHPSAKGTMA
PVYGMAASVPFRGLVSDLLKKYMDLLYKV

Sequences:

>Translated_509_residues
MALPDLKALSLLNHVPPRLLSAAERYLKAVPGVKGLLNRETGSLLSELESGLKPYRGKMQSFDHLPPTGRSREDVLRELQ
ALESQEEYRWREGRVSGGVYNGDAEHIAFLNRVYALHSQSNPLHADLWPSATKFEAEVVAMTASMLGADVANAGQPEEAR
ICGAMSSGGTESIMLAMKTYRDWARETKGITRPEMVAPASAHPAFDKAAHYFGIKMVRVPVGPDYRADVAAMRKAINRNT
IVLIGSAPGFPHGVIDPIEALSEIARKKRIGFHTDACLGGFVLPFAMKLGYDVPPFDFRLPGVTSMSVDTHKFGYAAKGS
SVVLYRGTELRSHQYFTATDWPGGIYFSPTFSGSRPGALIASAWAALVSTGEQGYLDATRSILETAAAIKKGIRDIPELH
VLGDPLFVIAFGSESVDIFQVMERMSEHGWNLNGLHKPSAVHLCVTLRHTQPGVAEQFLVDLRDAVDYVKSHPSAKGTMA
PVYGMAASVPFRGLVSDLLKKYMDLLYKV
>Mature_508_residues
ALPDLKALSLLNHVPPRLLSAAERYLKAVPGVKGLLNRETGSLLSELESGLKPYRGKMQSFDHLPPTGRSREDVLRELQA
LESQEEYRWREGRVSGGVYNGDAEHIAFLNRVYALHSQSNPLHADLWPSATKFEAEVVAMTASMLGADVANAGQPEEARI
CGAMSSGGTESIMLAMKTYRDWARETKGITRPEMVAPASAHPAFDKAAHYFGIKMVRVPVGPDYRADVAAMRKAINRNTI
VLIGSAPGFPHGVIDPIEALSEIARKKRIGFHTDACLGGFVLPFAMKLGYDVPPFDFRLPGVTSMSVDTHKFGYAAKGSS
VVLYRGTELRSHQYFTATDWPGGIYFSPTFSGSRPGALIASAWAALVSTGEQGYLDATRSILETAAAIKKGIRDIPELHV
LGDPLFVIAFGSESVDIFQVMERMSEHGWNLNGLHKPSAVHLCVTLRHTQPGVAEQFLVDLRDAVDYVKSHPSAKGTMAP
VYGMAASVPFRGLVSDLLKKYMDLLYKV

Specific function: Cleaves phosphorylated sphingoid bases (PSBs), such as sphingosine-1-phosphate, into fatty aldehydes and phosphoethanolamine. Possibly implicated in influencing the macrophage autophagy pathway [H]

COG id: COG0076

COG function: function code E; Glutamate decarboxylase and related PLP-dependent proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the group II decarboxylase family. Sphingosine-1-phosphate lyase subfamily [H]

Homologues:

Organism=Homo sapiens, GI31982936, Length=446, Percent_Identity=48.2062780269058, Blast_Score=420, Evalue=1e-117,
Organism=Escherichia coli, GI1789934, Length=405, Percent_Identity=24.1975308641975, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1787769, Length=405, Percent_Identity=24.1975308641975, Blast_Score=94, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17557272, Length=489, Percent_Identity=39.6728016359918, Blast_Score=352, Evalue=2e-97,
Organism=Caenorhabditis elegans, GI17543922, Length=485, Percent_Identity=38.9690721649485, Blast_Score=352, Evalue=2e-97,
Organism=Caenorhabditis elegans, GI25148342, Length=479, Percent_Identity=34.446764091858, Blast_Score=288, Evalue=5e-78,
Organism=Saccharomyces cerevisiae, GI6320500, Length=494, Percent_Identity=40.6882591093117, Blast_Score=375, Evalue=1e-104,
Organism=Saccharomyces cerevisiae, GI6323905, Length=287, Percent_Identity=26.4808362369338, Blast_Score=87, Evalue=9e-18,
Organism=Drosophila melanogaster, GI21355963, Length=487, Percent_Identity=44.1478439425051, Blast_Score=438, Evalue=1e-123,
Organism=Drosophila melanogaster, GI24654344, Length=487, Percent_Identity=44.1478439425051, Blast_Score=438, Evalue=1e-123,

Paralogues:

None

Copy number: 3820 Molecules/Cell In: Early Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002129
- InterPro:   IPR015424
- InterPro:   IPR015421 [H]

Pfam domain/function: PF00282 Pyridoxal_deC [H]

EC number: =4.1.2.27 [H]

Molecular weight: Translated: 55540; Mature: 55409

Theoretical pI: Translated: 8.41; Mature: 8.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALPDLKALSLLNHVPPRLLSAAERYLKAVPGVKGLLNRETGSLLSELESGLKPYRGKMQ
CCCCCHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHCCHHHHHHHHHHHCCCHHHHHHH
SFDHLPPTGRSREDVLRELQALESQEEYRWREGRVSGGVYNGDAEHIAFLNRVYALHSQS
HCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCHHHHHHHHHHHHHHCCC
NPLHADLWPSATKFEAEVVAMTASMLGADVANAGQPEEARICGAMSSGGTESIMLAMKTY
CCCEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHEECCCCCCCHHHHHHHHHH
RDWARETKGITRPEMVAPASAHPAFDKAAHYFGIKMVRVPVGPDYRADVAAMRKAINRNT
HHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHCEEEEEECCCCCCHHHHHHHHHHCCCCE
IVLIGSAPGFPHGVIDPIEALSEIARKKRIGFHTDACLGGFVLPFAMKLGYDVPPFDFRL
EEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEC
PGVTSMSVDTHKFGYAAKGSSVVLYRGTELRSHQYFTATDWPGGIYFSPTFSGSRPGALI
CCCCCCCCCCHHCCCCCCCCEEEEEECCCCCCCCEEEECCCCCCEEECCCCCCCCCCHHH
ASAWAALVSTGEQGYLDATRSILETAAAIKKGIRDIPELHVLGDPLFVIAFGSESVDIFQ
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCEEEEEECCCCHHHHH
VMERMSEHGWNLNGLHKPSAVHLCVTLRHTQPGVAEQFLVDLRDAVDYVKSHPSAKGTMA
HHHHHHHCCCCCCCCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCC
PVYGMAASVPFRGLVSDLLKKYMDLLYKV
HHHHHHHCCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
ALPDLKALSLLNHVPPRLLSAAERYLKAVPGVKGLLNRETGSLLSELESGLKPYRGKMQ
CCCCHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHCCHHHHHHHHHHHCCCHHHHHHH
SFDHLPPTGRSREDVLRELQALESQEEYRWREGRVSGGVYNGDAEHIAFLNRVYALHSQS
HCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCHHHHHHHHHHHHHHCCC
NPLHADLWPSATKFEAEVVAMTASMLGADVANAGQPEEARICGAMSSGGTESIMLAMKTY
CCCEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHEECCCCCCCHHHHHHHHHH
RDWARETKGITRPEMVAPASAHPAFDKAAHYFGIKMVRVPVGPDYRADVAAMRKAINRNT
HHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHCEEEEEECCCCCCHHHHHHHHHHCCCCE
IVLIGSAPGFPHGVIDPIEALSEIARKKRIGFHTDACLGGFVLPFAMKLGYDVPPFDFRL
EEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEC
PGVTSMSVDTHKFGYAAKGSSVVLYRGTELRSHQYFTATDWPGGIYFSPTFSGSRPGALI
CCCCCCCCCCHHCCCCCCCCEEEEEECCCCCCCCEEEECCCCCCEEECCCCCCCCCCHHH
ASAWAALVSTGEQGYLDATRSILETAAAIKKGIRDIPELHVLGDPLFVIAFGSESVDIFQ
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCEEEEEECCCCHHHHH
VMERMSEHGWNLNGLHKPSAVHLCVTLRHTQPGVAEQFLVDLRDAVDYVKSHPSAKGTMA
HHHHHHHCCCCCCCCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCC
PVYGMAASVPFRGLVSDLLKKYMDLLYKV
HHHHHHHCCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA