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The map label for this gene is xth [H]

Identifier: 108759762

GI number: 108759762

Start: 6469430

End: 6470200

Strand: Reverse

Name: xth [H]

Synonym: MXAN_5178

Alternate gene names: 108759762

Gene position: 6470200-6469430 (Counterclockwise)

Preceding gene: 108761115

Following gene: 108759633

Centisome position: 70.79

GC content: 66.8

Gene sequence:

>771_bases
ATGAAGATCGCCACCTGGAACGTGAACTCGGTGCGGGCCCGTCAGGAACGGCTGCTCGCCTGGTTGAAGAAGGCCCAGCC
GGATGTCCTCTGCCTGCAGGAGCTCAAGTGCGTGGACGACGACTTCCCCATGGAGGCCGTGCGGGAGTTGGGCTACCACG
CCGCCGTCCACGGGCAGAAGACGTACAACGGCGTCGCCATCCTCGCGAAGGAGGAGCCGAAGGACGTCGTGAAGGGCCTG
TCCGACGGCGTGGATGATTCGCATGCGCGCCTCATCGCGGCGACCGTGGGCGGCATCCGCGTGGTGAGCGCGTACGCGCC
CAATGGCCAGTCGGTGGACTCGCCGCAGTACCAGTACAAGCTGGAGTGGTACGGCCGGCTGCGGCGCTACCTGGACACAC
GGCACAAGCCCGACGAGCCCCTGGTGCTGGGCGGGGACTGGAACGTGGCGCCCGAGGACATCGACACCTATGACCCGAAG
TTGTGGGAAGGCCAGACGCTCTTCACGCTGAAGGAGCGGGATGCCCTCCAGCGCCTGGGCGCCTTCGGGCTTTCGGATGC
CTTCCGCAGGCTGCACCCTGGCGTTCAGAAGTTCACCTGGTGGGATTACCGCATGCTCGGCTTCCCGAAGAACCTGGGCC
TGCGCATCGACCACCTCTACGTGACGGCGCCCGTGGCCGAACGACTGACGGTCGTGGACGTGGACCGCGAGGAGCGCAAG
GGCAAGCAGCCCTCGGACCACGCTCCCGTGTGGCTCGAACTGCGCGACTGA

Upstream 100 bases:

>100_bases
GCATGCTCCCAGAGGGACGGAGGGGGCTGTGTGGCCCTGTGGCCACAGGGCCGGGGAAAAGCATGGCGGGGGGCGCGGGC
GGCATGCGACGGTCCGCGCG

Downstream 100 bases:

>100_bases
AGCGGTATTCGCGAGCGGCCATGTCATCGCTGGCATGGCCGCTCTCCACGAGCACCAGGCACGTGGTGGGATTCGTGCGA
TGCTCCAGGACCGAAGGCCA

Product: exodeoxyribonuclease III

Products: NA

Alternate protein names: EXO III; Exonuclease III [H]

Number of amino acids: Translated: 256; Mature: 256

Protein sequence:

>256_residues
MKIATWNVNSVRARQERLLAWLKKAQPDVLCLQELKCVDDDFPMEAVRELGYHAAVHGQKTYNGVAILAKEEPKDVVKGL
SDGVDDSHARLIAATVGGIRVVSAYAPNGQSVDSPQYQYKLEWYGRLRRYLDTRHKPDEPLVLGGDWNVAPEDIDTYDPK
LWEGQTLFTLKERDALQRLGAFGLSDAFRRLHPGVQKFTWWDYRMLGFPKNLGLRIDHLYVTAPVAERLTVVDVDREERK
GKQPSDHAPVWLELRD

Sequences:

>Translated_256_residues
MKIATWNVNSVRARQERLLAWLKKAQPDVLCLQELKCVDDDFPMEAVRELGYHAAVHGQKTYNGVAILAKEEPKDVVKGL
SDGVDDSHARLIAATVGGIRVVSAYAPNGQSVDSPQYQYKLEWYGRLRRYLDTRHKPDEPLVLGGDWNVAPEDIDTYDPK
LWEGQTLFTLKERDALQRLGAFGLSDAFRRLHPGVQKFTWWDYRMLGFPKNLGLRIDHLYVTAPVAERLTVVDVDREERK
GKQPSDHAPVWLELRD
>Mature_256_residues
MKIATWNVNSVRARQERLLAWLKKAQPDVLCLQELKCVDDDFPMEAVRELGYHAAVHGQKTYNGVAILAKEEPKDVVKGL
SDGVDDSHARLIAATVGGIRVVSAYAPNGQSVDSPQYQYKLEWYGRLRRYLDTRHKPDEPLVLGGDWNVAPEDIDTYDPK
LWEGQTLFTLKERDALQRLGAFGLSDAFRRLHPGVQKFTWWDYRMLGFPKNLGLRIDHLYVTAPVAERLTVVDVDREERK
GKQPSDHAPVWLELRD

Specific function: Major apurinic-apyrimidinic endonuclease of E.coli. It removes the damaged DNA at cytosines and guanines by cleaving on the 3'-side of the AP site by a beta-elimination reaction [H]

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=262, Percent_Identity=34.3511450381679, Blast_Score=134, Evalue=1e-31,
Organism=Homo sapiens, GI18375503, Length=262, Percent_Identity=34.3511450381679, Blast_Score=134, Evalue=1e-31,
Organism=Homo sapiens, GI18375501, Length=262, Percent_Identity=34.3511450381679, Blast_Score=134, Evalue=1e-31,
Organism=Escherichia coli, GI1788046, Length=267, Percent_Identity=40.8239700374532, Blast_Score=197, Evalue=7e-52,
Organism=Caenorhabditis elegans, GI71989536, Length=261, Percent_Identity=29.8850574712644, Blast_Score=103, Evalue=7e-23,
Organism=Drosophila melanogaster, GI221330655, Length=267, Percent_Identity=32.5842696629214, Blast_Score=114, Evalue=6e-26,
Organism=Drosophila melanogaster, GI17136678, Length=267, Percent_Identity=32.5842696629214, Blast_Score=113, Evalue=9e-26,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 29263; Mature: 29263

Theoretical pI: Translated: 6.89; Mature: 6.89

Prosite motif: PS00726 AP_NUCLEASE_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIATWNVNSVRARQERLLAWLKKAQPDVLCLQELKCVDDDFPMEAVRELGYHAAVHGQK
CEEEEECCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHCCCCEEECCCC
TYNGVAILAKEEPKDVVKGLSDGVDDSHARLIAATVGGIRVVSAYAPNGQSVDSPQYQYK
CCCCEEEEECCCHHHHHHHHHCCCCCCHHEEEEEECCCEEEEEEECCCCCCCCCCCEEEE
LEWYGRLRRYLDTRHKPDEPLVLGGDWNVAPEDIDTYDPKLWEGQTLFTLKERDALQRLG
HHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHH
AFGLSDAFRRLHPGVQKFTWWDYRMLGFPKNLGLRIDHLYVTAPVAERLTVVDVDREERK
CCCHHHHHHHHCCCHHHCEEECEEEECCCCCCCEEEEEEEEECCHHHCEEEEECCHHHHC
GKQPSDHAPVWLELRD
CCCCCCCCCEEEEECC
>Mature Secondary Structure
MKIATWNVNSVRARQERLLAWLKKAQPDVLCLQELKCVDDDFPMEAVRELGYHAAVHGQK
CEEEEECCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHCCCCEEECCCC
TYNGVAILAKEEPKDVVKGLSDGVDDSHARLIAATVGGIRVVSAYAPNGQSVDSPQYQYK
CCCCEEEEECCCHHHHHHHHHCCCCCCHHEEEEEECCCEEEEEEECCCCCCCCCCCEEEE
LEWYGRLRRYLDTRHKPDEPLVLGGDWNVAPEDIDTYDPKLWEGQTLFTLKERDALQRLG
HHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHH
AFGLSDAFRRLHPGVQKFTWWDYRMLGFPKNLGLRIDHLYVTAPVAERLTVVDVDREERK
CCCHHHHHHHHCCCHHHCEEECEEEECCCCCCCEEEEEEEEECCHHHCEEEEECCHHHHC
GKQPSDHAPVWLELRD
CCCCCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]