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The map label for this gene is ligA

Identifier: 108759370

GI number: 108759370

Start: 7002285

End: 7004303

Strand: Direct

Name: ligA

Synonym: MXAN_5637

Alternate gene names: 108759370

Gene position: 7002285-7004303 (Clockwise)

Preceding gene: 108762102

Following gene: 108757088

Centisome position: 76.61

GC content: 67.71

Gene sequence:

>2019_bases
GTGGACGACTTCCAGAAAGCCGCAGCCCGAGCCCGTGAGCTCCACCGTGAGCTGGCGCACCACAACTACCGTTACTACGT
CCTCGACTCGCCCGAGGTCAGCGACGCGCAATACGACAAGCTGATGCGCGAGCTGCAGGACCTGGAGGCGAAGCACCCGA
GCCTCCAGACGCCGGACTCGCCCACCCAGCGCGTGGGCGGCGCGGCGGCCGAGGAGTTTGGCGAGGTGGTGCACCGGGCG
CCCATGATCTCGCTGGCCAACATCTTCGAGGACCAGGGCCTCACTGAGTTCGACGAGCGCATCCGCAAGCTGGTGGGCCT
GCCGGGCATCGCCTACGTCTGCGAGCCCAAGCTGGACGGACTCGCCATCGCGTTGCGTTACGAAAAGGGCGCGTTCGTCC
AGGGCGCCACCCGGGGCGACGGCACCACTGGCGAGGACGTCACCTCGAACCTGCGCACCATCCGAAGCCTGCCCATGTCG
CTGTTCCCCCAGGACGACGTGAAGGTGCCGGACGTGCTGGAGGTCCGCGGTGAGGTCTTCATCCGCAAGAAGGACTTCCA
GAAGCTCAACGAGAAGCGCGAGGAGGAAGGCGAGCCGCTCTTCGCCAACCCGCGCAACGCCGCCGCCGGCAGCCTGCGCC
AGTTGGACCCGCGGATGACGGCCGCCCGGCCCCTCTCCGTGTTCCTCTACGAATGCGTCCCGGGCGAAGGCGTGCCCGTC
TTCAAGACGCACATCGAGAAGCTGGAGTACCTCAAGACGCTGGGCCTGCCCATCAACCAGTACCGCCGCGCCGAGGGCCT
GGAGGGCGTGCGCCAGGCCTATGACGCCTCCCTCAAGGGCCGCCACGAGCTGCCCTTCGAAGTGGACGGCATGGTGGTGA
AGGTGGATGACGAGGACCAGCGCAAGCGCCTGGGCCAGGTCTCCAAGAGCCCCCGCTGGGCGGTGGCTTACAAGTTCCCT
CCGGAGGAGGAGTCCACGGAGGTGATGGACATCGGCATCCAGGTGGGCCGCACGGGCGCGCTGACGCCGGTGGCACACCT
CAAGCCGGTGAAGGTGGGCGGCGTCACGGTGGCGCGCGCCACGCTGCACAACGAGGACGAGCTGCGCCGCAAGGACGTGC
GCAAGGGCGACACCGTCTTCGTGCGCCGCGCCGGTGACGTGATTCCGGAAATCGTCTCCGTGGTGCTGTCCAAGCGCCCC
GCGGACTCCGCGCCCTTCGAGTTCCCCAAGCACTGTCCTGTCTGCGATGCGGTGGCGACCAAGGACGAGGACGGCGCCAT
CATCCGCTGCACGGGCGCTTCCTGCCCCGCACAACTGGTGGAGAAGATCCGCCACTTCGCCAGCCGCCTGGCCATGGACA
TCGAAGGGCTGGGCGACAAGCTGGCCGCGCAGTTGGTGTCCACCGGCAGGGTGAAGGCGTTCGCGGACCTCTACGCGCTC
ACCAAGGAGGACCTGCTGACGCTGGAGCGCATGGGCGACAAGAGCGCGGACAACCTCATCGCGTCACTGGAGCGCTCCAA
GCAGACCACACAGCGCCGCTTCCTCTATTCCCTGGGCATCCGCCACGTGGGTGACGCCACCGCCAAGGCGCTGGCGGAGG
CCTTCCCCCGGTCAGAGATGCTCTTCGAGGCCAGCCTGGAGGACATCTCCCGCGTAAAGGACGTAGGCCCCATCATGGCC
CAGGTCATCCACACCTTCTTCCAGGAGCCCCAGAATCAGGAGGCCATCCGCGCGCTCCTGGCGGCGGGGGTCCAGCCCGC
GGCGCCCCAGGTCGCCACGGGAGGCCCCTTCGTGGGCAAGTCGGTGGTGCTCACCGGCGCGATGACGGGTATGACGCGGG
AGCAGGCCAAGGAAGAGGTCGAGCGCCGGGGCGGCAAGGTCGCCGGAAGTGTCTCGCGCAAGACCGATTTCGTCGTGGCC
GGTGAGGATGCGGGCAGCAAACTGAAGAAGGCCCAGGAACTCGGGGTAAGAATCCTGGACGAGCAGGCGTTCCTGCAGAT
GCTGCAGACGAACGCCTAG

Upstream 100 bases:

>100_bases
GCGGAGACGACGACCGGACCTTGTCAGTCGGGAACCGTAGAGTGGCCGCCTTTCGAGGCACGCGCCGACGCGCCGTGTTT
TGGAGTAAGGACCGCGAACC

Downstream 100 bases:

>100_bases
AGGATGGGGCAGTCCATGAGTGGCACGCGGCGAGCCTCGCTGCGCATCGAAGGCAAGGTGCAGGGCGTCTTCTTCAGGGA
GAGCGCCCGCGTTGAAGCCA

Product: NAD-dependent DNA ligase

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+]

Number of amino acids: Translated: 672; Mature: 672

Protein sequence:

>672_residues
MDDFQKAAARARELHRELAHHNYRYYVLDSPEVSDAQYDKLMRELQDLEAKHPSLQTPDSPTQRVGGAAAEEFGEVVHRA
PMISLANIFEDQGLTEFDERIRKLVGLPGIAYVCEPKLDGLAIALRYEKGAFVQGATRGDGTTGEDVTSNLRTIRSLPMS
LFPQDDVKVPDVLEVRGEVFIRKKDFQKLNEKREEEGEPLFANPRNAAAGSLRQLDPRMTAARPLSVFLYECVPGEGVPV
FKTHIEKLEYLKTLGLPINQYRRAEGLEGVRQAYDASLKGRHELPFEVDGMVVKVDDEDQRKRLGQVSKSPRWAVAYKFP
PEEESTEVMDIGIQVGRTGALTPVAHLKPVKVGGVTVARATLHNEDELRRKDVRKGDTVFVRRAGDVIPEIVSVVLSKRP
ADSAPFEFPKHCPVCDAVATKDEDGAIIRCTGASCPAQLVEKIRHFASRLAMDIEGLGDKLAAQLVSTGRVKAFADLYAL
TKEDLLTLERMGDKSADNLIASLERSKQTTQRRFLYSLGIRHVGDATAKALAEAFPRSEMLFEASLEDISRVKDVGPIMA
QVIHTFFQEPQNQEAIRALLAAGVQPAAPQVATGGPFVGKSVVLTGAMTGMTREQAKEEVERRGGKVAGSVSRKTDFVVA
GEDAGSKLKKAQELGVRILDEQAFLQMLQTNA

Sequences:

>Translated_672_residues
MDDFQKAAARARELHRELAHHNYRYYVLDSPEVSDAQYDKLMRELQDLEAKHPSLQTPDSPTQRVGGAAAEEFGEVVHRA
PMISLANIFEDQGLTEFDERIRKLVGLPGIAYVCEPKLDGLAIALRYEKGAFVQGATRGDGTTGEDVTSNLRTIRSLPMS
LFPQDDVKVPDVLEVRGEVFIRKKDFQKLNEKREEEGEPLFANPRNAAAGSLRQLDPRMTAARPLSVFLYECVPGEGVPV
FKTHIEKLEYLKTLGLPINQYRRAEGLEGVRQAYDASLKGRHELPFEVDGMVVKVDDEDQRKRLGQVSKSPRWAVAYKFP
PEEESTEVMDIGIQVGRTGALTPVAHLKPVKVGGVTVARATLHNEDELRRKDVRKGDTVFVRRAGDVIPEIVSVVLSKRP
ADSAPFEFPKHCPVCDAVATKDEDGAIIRCTGASCPAQLVEKIRHFASRLAMDIEGLGDKLAAQLVSTGRVKAFADLYAL
TKEDLLTLERMGDKSADNLIASLERSKQTTQRRFLYSLGIRHVGDATAKALAEAFPRSEMLFEASLEDISRVKDVGPIMA
QVIHTFFQEPQNQEAIRALLAAGVQPAAPQVATGGPFVGKSVVLTGAMTGMTREQAKEEVERRGGKVAGSVSRKTDFVVA
GEDAGSKLKKAQELGVRILDEQAFLQMLQTNA
>Mature_672_residues
MDDFQKAAARARELHRELAHHNYRYYVLDSPEVSDAQYDKLMRELQDLEAKHPSLQTPDSPTQRVGGAAAEEFGEVVHRA
PMISLANIFEDQGLTEFDERIRKLVGLPGIAYVCEPKLDGLAIALRYEKGAFVQGATRGDGTTGEDVTSNLRTIRSLPMS
LFPQDDVKVPDVLEVRGEVFIRKKDFQKLNEKREEEGEPLFANPRNAAAGSLRQLDPRMTAARPLSVFLYECVPGEGVPV
FKTHIEKLEYLKTLGLPINQYRRAEGLEGVRQAYDASLKGRHELPFEVDGMVVKVDDEDQRKRLGQVSKSPRWAVAYKFP
PEEESTEVMDIGIQVGRTGALTPVAHLKPVKVGGVTVARATLHNEDELRRKDVRKGDTVFVRRAGDVIPEIVSVVLSKRP
ADSAPFEFPKHCPVCDAVATKDEDGAIIRCTGASCPAQLVEKIRHFASRLAMDIEGLGDKLAAQLVSTGRVKAFADLYAL
TKEDLLTLERMGDKSADNLIASLERSKQTTQRRFLYSLGIRHVGDATAKALAEAFPRSEMLFEASLEDISRVKDVGPIMA
QVIHTFFQEPQNQEAIRALLAAGVQPAAPQVATGGPFVGKSVVLTGAMTGMTREQAKEEVERRGGKVAGSVSRKTDFVVA
GEDAGSKLKKAQELGVRILDEQAFLQMLQTNA

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain

Homologues:

Organism=Homo sapiens, GI32528306, Length=100, Percent_Identity=39, Blast_Score=69, Evalue=1e-11,
Organism=Escherichia coli, GI1788750, Length=663, Percent_Identity=50.9803921568627, Blast_Score=655, Evalue=0.0,
Organism=Escherichia coli, GI87082305, Length=529, Percent_Identity=23.2514177693762, Blast_Score=104, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNLJ_MYXXD (Q1D0P7)

Other databases:

- EMBL:   CP000113
- RefSeq:   YP_633776.1
- ProteinModelPortal:   Q1D0P7
- SMR:   Q1D0P7
- STRING:   Q1D0P7
- GeneID:   4101032
- GenomeReviews:   CP000113_GR
- KEGG:   mxa:MXAN_5637
- TIGR:   MXAN_5637
- eggNOG:   COG0272
- HOGENOM:   HBG620317
- OMA:   IKHFASR
- PhylomeDB:   Q1D0P7
- BioCyc:   MXAN246197:MXAN_5637-MONOMER
- GO:   GO:0005622
- HAMAP:   MF_01588
- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149
- Gene3D:   G3DSA:2.40.50.140
- PIRSF:   PIRSF001604
- SMART:   SM00292
- SMART:   SM00278
- SMART:   SM00532
- TIGRFAMs:   TIGR00575

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD; SSF52113 BRCT; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like

EC number: =6.5.1.2

Molecular weight: Translated: 74046; Mature: 74046

Theoretical pI: Translated: 6.47; Mature: 6.47

Prosite motif: PS50172 BRCT; PS01055 DNA_LIGASE_N1; PS01056 DNA_LIGASE_N2

Important sites: ACT_SITE 117-117 BINDING 115-115 BINDING 138-138 BINDING 178-178 BINDING 294-294 BINDING 318-318

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDDFQKAAARARELHRELAHHNYRYYVLDSPEVSDAQYDKLMRELQDLEAKHPSLQTPDS
CCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCC
PTQRVGGAAAEEFGEVVHRAPMISLANIFEDQGLTEFDERIRKLVGLPGIAYVCEPKLDG
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCEEEEECCCCCC
LAIALRYEKGAFVQGATRGDGTTGEDVTSNLRTIRSLPMSLFPQDDVKVPDVLEVRGEVF
EEEEEEECCCCEEECCCCCCCCCCHHHHHHHHHHHHCCHHCCCCCCCCCCHHHHHCCHHE
IRKKDFQKLNEKREEEGEPLFANPRNAAAGSLRQLDPRMTAARPLSVFLYECVPGEGVPV
EEHHHHHHHHHHHHHCCCCCCCCCCCHHHCHHHHCCCHHHHCCHHHHHHHHHCCCCCCHH
FKTHIEKLEYLKTLGLPINQYRRAEGLEGVRQAYDASLKGRHELPFEVDGMVVKVDDEDQ
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCEEEEECCHHH
RKRLGQVSKSPRWAVAYKFPPEEESTEVMDIGIQVGRTGALTPVAHLKPVKVGGVTVARA
HHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHEECCCCCCCCHHHCCEEEECCEEEEHH
TLHNEDELRRKDVRKGDTVFVRRAGDVIPEIVSVVLSKRPADSAPFEFPKHCPVCDAVAT
HHCCHHHHHHHHCCCCCEEEEECHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHCC
KDEDGAIIRCTGASCPAQLVEKIRHFASRLAMDIEGLGDKLAAQLVSTGRVKAFADLYAL
CCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
TKEDLLTLERMGDKSADNLIASLERSKQTTQRRFLYSLGIRHVGDATAKALAEAFPRSEM
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHH
LFEASLEDISRVKDVGPIMAQVIHTFFQEPQNQEAIRALLAAGVQPAAPQVATGGPFVGK
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCC
SVVLTGAMTGMTREQAKEEVERRGGKVAGSVSRKTDFVVAGEDAGSKLKKAQELGVRILD
HHHHHHHHHCCHHHHHHHHHHHCCCCEEECCCCCCCEEEECCCCCHHHHHHHHHCCEEEC
EQAFLQMLQTNA
HHHHHHHHHHCC
>Mature Secondary Structure
MDDFQKAAARARELHRELAHHNYRYYVLDSPEVSDAQYDKLMRELQDLEAKHPSLQTPDS
CCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCC
PTQRVGGAAAEEFGEVVHRAPMISLANIFEDQGLTEFDERIRKLVGLPGIAYVCEPKLDG
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCEEEEECCCCCC
LAIALRYEKGAFVQGATRGDGTTGEDVTSNLRTIRSLPMSLFPQDDVKVPDVLEVRGEVF
EEEEEEECCCCEEECCCCCCCCCCHHHHHHHHHHHHCCHHCCCCCCCCCCHHHHHCCHHE
IRKKDFQKLNEKREEEGEPLFANPRNAAAGSLRQLDPRMTAARPLSVFLYECVPGEGVPV
EEHHHHHHHHHHHHHCCCCCCCCCCCHHHCHHHHCCCHHHHCCHHHHHHHHHCCCCCCHH
FKTHIEKLEYLKTLGLPINQYRRAEGLEGVRQAYDASLKGRHELPFEVDGMVVKVDDEDQ
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCEEEEECCHHH
RKRLGQVSKSPRWAVAYKFPPEEESTEVMDIGIQVGRTGALTPVAHLKPVKVGGVTVARA
HHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHEECCCCCCCCHHHCCEEEECCEEEEHH
TLHNEDELRRKDVRKGDTVFVRRAGDVIPEIVSVVLSKRPADSAPFEFPKHCPVCDAVAT
HHCCHHHHHHHHCCCCCEEEEECHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHCC
KDEDGAIIRCTGASCPAQLVEKIRHFASRLAMDIEGLGDKLAAQLVSTGRVKAFADLYAL
CCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
TKEDLLTLERMGDKSADNLIASLERSKQTTQRRFLYSLGIRHVGDATAKALAEAFPRSEM
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHH
LFEASLEDISRVKDVGPIMAQVIHTFFQEPQNQEAIRALLAAGVQPAAPQVATGGPFVGK
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCC
SVVLTGAMTGMTREQAKEEVERRGGKVAGSVSRKTDFVVAGEDAGSKLKKAQELGVRILD
HHHHHHHHHCCHHHHHHHHHHHCCCCEEECCCCCCCEEEECCCCCHHHHHHHHHCCEEEC
EQAFLQMLQTNA
HHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA