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The map label for this gene is kinE [H]

Identifier: 108759200

GI number: 108759200

Start: 6294467

End: 6296014

Strand: Direct

Name: kinE [H]

Synonym: MXAN_5034

Alternate gene names: 108759200

Gene position: 6294467-6296014 (Clockwise)

Preceding gene: 108761223

Following gene: 108761135

Centisome position: 68.87

GC content: 70.03

Gene sequence:

>1548_bases
ATGCCCGTGAAGCTCTCTCTCGCGACACGCATCTTCCTGGGCTACGCCGTGGTGCTCGGCACCTTCGGGCTGGTGTCCCT
GTTCAGCGTGACGGAGCTCCACCGCAACCGGCTGGAAATCCGGCTGGTGAGCCAGGGCTACCTCCAGCTCTCCCAGGACG
CCGCGGAGCTGGAGACCTTCCACGCCACCCAGGAGAAGGACACCGAGCGCCTGATTCAAGAAGGCAACGTCGAAATCCGC
CGCGCCTTCATCCGGCTGGCCCGGACCTACAACTCGCCCCTCATGACCCAGCGGCTCGCCGCCGCCCAGGCCAAGGCGCA
TGAGGTGCTGGCCTCCGCCCCCGACAGCGAGGGGCCCTTCATCCGGGGCCTGGAGGACCGCTTCGGCGAGCTCCAGGCCC
GCTACCGCGACTACGGCCGCGCCGCGGAGGCCGTCTTCGCCGTCCTGTCCACCGAGAATCCGAACCGGGAACAGGTCGCC
GTCGCCACCGCCGAGCTGCGGCAGGAGGAGAACGCCATCGGCCGAGAGATTCGCGTGCTGCGCGCCGCCCTGTCCAACCG
CATCCGCGAGCGCGTGGACGGCGCCGAGGAGCGCGAGCGCACCACCGGCCTCTTCATCATCGGCTTCTCCGTGGCCGCCA
TCGCCGTGGGCGTGGGCGCCACTGCATGGTCCGCCCGCACGCTGCGCCCCATGCGCAACCTCATCCGGGGCGTGTCCCGC
ATCGGCCGTGGTGACTACAACGCCCAGCTCGGCGTGCGCGGCGACGACGAGGTGGCCGTCCTGGCCCGGGAGTTCGACCA
GATGGCCCGCTCGCTCCAGGCGCGTGAGGCCCAGCTCAAGGCCCAGGCCGAGGCCCTCATGCGCGCGGAGCAACTGGCCG
CCGTGGGCCGCATCTCCGCGCAAATCGTCCACGAGGTGCGCAACCCCCTGTCCTCCATTGGCCTCAACGTGGAGTTGCTC
CAGGACGGGTTCGAAACCGCCCGCTTCGCGACCCCCGAGGACGCGGCCGAGGTGAAGGACCTCATCTCCGCCGTCACCCA
GGAGGTGGACCGCCTGGCGGATGTCACCGAGCAGTACCTGCGCATGGCCCGCCCGCCCCGGCCCGACCTGGACCCGCGCG
ACGTCACCGCCGTGCTGGACACCGTGCTCGACTTCACCCGAGAGGAGTTGGAGCGCGCCGGCGTGGAAGTGGTGCGCGAC
TTCGCCCTGGATACCCCGCATGTGCTCGCCGACGAGGGCCAGTTGCGGCAGGTCTTCCTCAACCTGCTGCGCAACAGCCG
CGAGGCCATGCCGTCCGGCGGCCGCCTCACCATCGTCACCCGTCCGGCGGAGGACGCCGTGGAAGTCACGGTGCGCGATA
CCGGACAGGGCATGACGGAGGATATCCGACAGCGCCTCTTCGAGCCCTTCTTCACCACCAAGGAGGGGGGCACGGGCCTG
GGCCTGGCCGTCAGCCAGCAAATCCTCCAGGTGCACGGTGGCTCGCTCTCCTGCCAGAGTATTCCCGGCCAGGGGACGGC
CTTCGTGTTAAGGCTTCCTCGCGCATGA

Upstream 100 bases:

>100_bases
CCCACTGGAGGTAGCCCCTCCCCCTCGGCGGCCCCCGAGCAGGCGCCTGCGCCCCCTGGAGACGAGACTTGCGGTTGGAC
GGCCCGCCACGTAGCGAGGA

Downstream 100 bases:

>100_bases
GCTTCACTACGTACCGGGACGTGCTGCCCTCCGGGCTGCGCGTCGTCACTATCGAGACGCCCCACCTCCACACCGCCCTG
CTCGCCGTCTACGTGCGGAC

Product: sensor histidine kinase

Products: NA

Alternate protein names: Sensor histidine kinase E [H]

Number of amino acids: Translated: 515; Mature: 514

Protein sequence:

>515_residues
MPVKLSLATRIFLGYAVVLGTFGLVSLFSVTELHRNRLEIRLVSQGYLQLSQDAAELETFHATQEKDTERLIQEGNVEIR
RAFIRLARTYNSPLMTQRLAAAQAKAHEVLASAPDSEGPFIRGLEDRFGELQARYRDYGRAAEAVFAVLSTENPNREQVA
VATAELRQEENAIGREIRVLRAALSNRIRERVDGAEERERTTGLFIIGFSVAAIAVGVGATAWSARTLRPMRNLIRGVSR
IGRGDYNAQLGVRGDDEVAVLAREFDQMARSLQAREAQLKAQAEALMRAEQLAAVGRISAQIVHEVRNPLSSIGLNVELL
QDGFETARFATPEDAAEVKDLISAVTQEVDRLADVTEQYLRMARPPRPDLDPRDVTAVLDTVLDFTREELERAGVEVVRD
FALDTPHVLADEGQLRQVFLNLLRNSREAMPSGGRLTIVTRPAEDAVEVTVRDTGQGMTEDIRQRLFEPFFTTKEGGTGL
GLAVSQQILQVHGGSLSCQSIPGQGTAFVLRLPRA

Sequences:

>Translated_515_residues
MPVKLSLATRIFLGYAVVLGTFGLVSLFSVTELHRNRLEIRLVSQGYLQLSQDAAELETFHATQEKDTERLIQEGNVEIR
RAFIRLARTYNSPLMTQRLAAAQAKAHEVLASAPDSEGPFIRGLEDRFGELQARYRDYGRAAEAVFAVLSTENPNREQVA
VATAELRQEENAIGREIRVLRAALSNRIRERVDGAEERERTTGLFIIGFSVAAIAVGVGATAWSARTLRPMRNLIRGVSR
IGRGDYNAQLGVRGDDEVAVLAREFDQMARSLQAREAQLKAQAEALMRAEQLAAVGRISAQIVHEVRNPLSSIGLNVELL
QDGFETARFATPEDAAEVKDLISAVTQEVDRLADVTEQYLRMARPPRPDLDPRDVTAVLDTVLDFTREELERAGVEVVRD
FALDTPHVLADEGQLRQVFLNLLRNSREAMPSGGRLTIVTRPAEDAVEVTVRDTGQGMTEDIRQRLFEPFFTTKEGGTGL
GLAVSQQILQVHGGSLSCQSIPGQGTAFVLRLPRA
>Mature_514_residues
PVKLSLATRIFLGYAVVLGTFGLVSLFSVTELHRNRLEIRLVSQGYLQLSQDAAELETFHATQEKDTERLIQEGNVEIRR
AFIRLARTYNSPLMTQRLAAAQAKAHEVLASAPDSEGPFIRGLEDRFGELQARYRDYGRAAEAVFAVLSTENPNREQVAV
ATAELRQEENAIGREIRVLRAALSNRIRERVDGAEERERTTGLFIIGFSVAAIAVGVGATAWSARTLRPMRNLIRGVSRI
GRGDYNAQLGVRGDDEVAVLAREFDQMARSLQAREAQLKAQAEALMRAEQLAAVGRISAQIVHEVRNPLSSIGLNVELLQ
DGFETARFATPEDAAEVKDLISAVTQEVDRLADVTEQYLRMARPPRPDLDPRDVTAVLDTVLDFTREELERAGVEVVRDF
ALDTPHVLADEGQLRQVFLNLLRNSREAMPSGGRLTIVTRPAEDAVEVTVRDTGQGMTEDIRQRLFEPFFTTKEGGTGLG
LAVSQQILQVHGGSLSCQSIPGQGTAFVLRLPRA

Specific function: Phosphorylates the sporulation-regulatory protein spo0A under biofilm growth conditions. Also able to weakly phosphorylate spo0F [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 PAS (PER-ARNT-SIM) domains [H]

Homologues:

Organism=Escherichia coli, GI1788549, Length=229, Percent_Identity=32.7510917030568, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI1790436, Length=229, Percent_Identity=32.7510917030568, Blast_Score=125, Evalue=6e-30,
Organism=Escherichia coli, GI1788393, Length=296, Percent_Identity=29.3918918918919, Blast_Score=111, Evalue=1e-25,
Organism=Escherichia coli, GI1790300, Length=250, Percent_Identity=28, Blast_Score=89, Evalue=1e-18,
Organism=Escherichia coli, GI1789149, Length=351, Percent_Identity=25.0712250712251, Blast_Score=82, Evalue=7e-17,
Organism=Escherichia coli, GI145693157, Length=249, Percent_Identity=28.9156626506024, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI87081816, Length=344, Percent_Identity=27.6162790697674, Blast_Score=79, Evalue=9e-16,
Organism=Escherichia coli, GI1786783, Length=289, Percent_Identity=25.2595155709343, Blast_Score=74, Evalue=3e-14,
Organism=Escherichia coli, GI1790346, Length=295, Percent_Identity=25.0847457627119, Blast_Score=73, Evalue=5e-14,
Organism=Escherichia coli, GI48994928, Length=228, Percent_Identity=28.5087719298246, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI1786600, Length=256, Percent_Identity=25, Blast_Score=67, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 56860; Mature: 56729

Theoretical pI: Translated: 5.35; Mature: 5.35

Prosite motif: PS50885 HAMP ; PS00050 RIBOSOMAL_L23 ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVKLSLATRIFLGYAVVLGTFGLVSLFSVTELHRNRLEIRLVSQGYLQLSQDAAELETF
CCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCHHHHHHHHHHHHHHH
HATQEKDTERLIQEGNVEIRRAFIRLARTYNSPLMTQRLAAAQAKAHEVLASAPDSEGPF
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCC
IRGLEDRFGELQARYRDYGRAAEAVFAVLSTENPNREQVAVATAELRQEENAIGREIRVL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
RAALSNRIRERVDGAEERERTTGLFIIGFSVAAIAVGVGATAWSARTLRPMRNLIRGVSR
HHHHHHHHHHHHCCHHHHHHHCCEEEEHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
IGRGDYNAQLGVRGDDEVAVLAREFDQMARSLQAREAQLKAQAEALMRAEQLAAVGRISA
HCCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QIVHEVRNPLSSIGLNVELLQDGFETARFATPEDAAEVKDLISAVTQEVDRLADVTEQYL
HHHHHHHHHHHHHCCCHHHHHCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
RMARPPRPDLDPRDVTAVLDTVLDFTREELERAGVEVVRDFALDTPHVLADEGQLRQVFL
HHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHH
NLLRNSREAMPSGGRLTIVTRPAEDAVEVTVRDTGQGMTEDIRQRLFEPFFTTKEGGTGL
HHHHHHHHCCCCCCEEEEEECCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCC
GLAVSQQILQVHGGSLSCQSIPGQGTAFVLRLPRA
HHHHHHHHHHHCCCCEEECCCCCCCCEEEEECCCC
>Mature Secondary Structure 
PVKLSLATRIFLGYAVVLGTFGLVSLFSVTELHRNRLEIRLVSQGYLQLSQDAAELETF
CEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCHHHHHHHHHHHHHHH
HATQEKDTERLIQEGNVEIRRAFIRLARTYNSPLMTQRLAAAQAKAHEVLASAPDSEGPF
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCC
IRGLEDRFGELQARYRDYGRAAEAVFAVLSTENPNREQVAVATAELRQEENAIGREIRVL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
RAALSNRIRERVDGAEERERTTGLFIIGFSVAAIAVGVGATAWSARTLRPMRNLIRGVSR
HHHHHHHHHHHHCCHHHHHHHCCEEEEHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
IGRGDYNAQLGVRGDDEVAVLAREFDQMARSLQAREAQLKAQAEALMRAEQLAAVGRISA
HCCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QIVHEVRNPLSSIGLNVELLQDGFETARFATPEDAAEVKDLISAVTQEVDRLADVTEQYL
HHHHHHHHHHHHHCCCHHHHHCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
RMARPPRPDLDPRDVTAVLDTVLDFTREELERAGVEVVRDFALDTPHVLADEGQLRQVFL
HHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHH
NLLRNSREAMPSGGRLTIVTRPAEDAVEVTVRDTGQGMTEDIRQRLFEPFFTTKEGGTGL
HHHHHHHHCCCCCCEEEEEECCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCC
GLAVSQQILQVHGGSLSCQSIPGQGTAFVLRLPRA
HHHHHHHHHHHCCCCEEECCCCCCCCEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]