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The map label for this gene is wcaJ [H]

Identifier: 108758966

GI number: 108758966

Start: 3423007

End: 3424401

Strand: Direct

Name: wcaJ [H]

Synonym: MXAN_2922

Alternate gene names: 108758966

Gene position: 3423007-3424401 (Clockwise)

Preceding gene: 108759676

Following gene: 108759526

Centisome position: 37.45

GC content: 64.3

Gene sequence:

>1395_bases
GTGTTCAGTCGTCTCCAGCGTTTCTACACGTCGATCAAAGTCGTCGCCGACATGGTGATGCTCGCGCTGGCATTCGTCCT
CGCGTACGCCACGCGTTTCTCCGGCATCGTGCCCGTCACGGAGGGGATACCTCCCTGGACGGACTCGTTCTTTTCGCTGC
TGATGGTGCTCGTCATCTTCCCGGTGACATTCCACCAGTCACGCCTGTACGGCACCAATCGTTCCCGGACGAATACGGGC
GAGGTGTTCGAGGTATTCAAGTCGACCATCACCGCGGCGCTCATCCTCGTGGCGGTGACGTACTTCGTTCGCGAGCGCTA
TTCGCGCCTCACGCTGGTCATCTTCATCGTCTATGCGTTCGCGCTGGTGACCTGCAGCCGGCTGGTGACTCGCTATCTGG
TGAGTGAAATCCGGCGCCGGGGCCACAACCTCAAGTCCATCCTCGTCATCGGCGCGGGGGACCTGGGACAGCGGGTCATC
GAGACAGTGGAGCACCACCGCGAGCTGGGCTTCCGGGTGATGGGCGTGCTGACGCTGCACCCGGAGAAGGTGGGCCAGTA
CGTGAATGACGTGCGCGTCATCGGGGTCGTGGACCAGGTGAACGAGGTGCTGGATTCGCACCCGGTGGACCAGGTCATCC
TCGCGCTGCCGCTGGCGGGCCACGCCCACGTCAAGGCGCTGATGGACAAGCTGGCCCTGCGCACCGTGGATGTGCGCGTG
GTGCCGGACCTGTACCAGTACATCACCCTCTATGGTGGCCTGGAGGAGTTCGGCGGGTTGCCCATCATCCGTCTCCAGGG
CGACCCGATGGAGGGCTGGAGCCGGGTGGCCAAGCGGGCCTTCGACATCCTCTTCTCGTTGCTGGCCATCCTCATCACCG
CGCCGCTCATGGCCGCCACCGCGCTGGCGGTGCGGCTCACCAGCCGTGGGCCCATGCTCTACCGTCAGGAGCGCATGGGC
ATGGATGGGCGCACCTTCCCCATCCTCAAGTTCCGCACCATGTGCATCGACGCCGAGCACGGCGGCGCGATGATGACCTG
CCCGGACGACCCGCGGCGCACGGTGATTGGCACGTTCCTGCGGAAGTACTCCCTGGACGAGCTCCCTCAGTTTTTCAACG
TCCTGCGCGGTGACATGAGCCTGGTAGGGCCGCGCCCGGAGCGCCCCGTCTTCATCGAGGAGTTCAAGCGGCAGATTCCG
CGCTACCACCTGCGGCACAAGGTGAAGGCGGGCATCACCGGCTGGGCGCAAATCAACGGCCTGCGCGGCCAGACGTGCAT
CGAGAAGCGCATCGAGTACGACCTGTACTACATCGAGAACTGGTCGCTGCTGATGGACCTGAAGATTCTCGTGCGCACGG
CGCTGGGCGGCTTCCTGTCGAAGAACGCCTACTGA

Upstream 100 bases:

>100_bases
GGCTATCAAACGGTGATGGGCAAAACGCGCGTCCGGATGAAGAGGGCGCGAGTCTTGCACCGGGGTGGCCACCCGTTGAT
TACGCCACAGGAGTACTTCG

Downstream 100 bases:

>100_bases
CGCCGAGAGCCCGGGCGGTGGCGCTCGCCACGGCCCGGGCCCGGGTGTTCAACGCCGCTGCGTGGTGACTACTGGATGTT
CCACACCACGGTGCCGGTGC

Product: sugar transferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 464; Mature: 464

Protein sequence:

>464_residues
MFSRLQRFYTSIKVVADMVMLALAFVLAYATRFSGIVPVTEGIPPWTDSFFSLLMVLVIFPVTFHQSRLYGTNRSRTNTG
EVFEVFKSTITAALILVAVTYFVRERYSRLTLVIFIVYAFALVTCSRLVTRYLVSEIRRRGHNLKSILVIGAGDLGQRVI
ETVEHHRELGFRVMGVLTLHPEKVGQYVNDVRVIGVVDQVNEVLDSHPVDQVILALPLAGHAHVKALMDKLALRTVDVRV
VPDLYQYITLYGGLEEFGGLPIIRLQGDPMEGWSRVAKRAFDILFSLLAILITAPLMAATALAVRLTSRGPMLYRQERMG
MDGRTFPILKFRTMCIDAEHGGAMMTCPDDPRRTVIGTFLRKYSLDELPQFFNVLRGDMSLVGPRPERPVFIEEFKRQIP
RYHLRHKVKAGITGWAQINGLRGQTCIEKRIEYDLYYIENWSLLMDLKILVRTALGGFLSKNAY

Sequences:

>Translated_464_residues
MFSRLQRFYTSIKVVADMVMLALAFVLAYATRFSGIVPVTEGIPPWTDSFFSLLMVLVIFPVTFHQSRLYGTNRSRTNTG
EVFEVFKSTITAALILVAVTYFVRERYSRLTLVIFIVYAFALVTCSRLVTRYLVSEIRRRGHNLKSILVIGAGDLGQRVI
ETVEHHRELGFRVMGVLTLHPEKVGQYVNDVRVIGVVDQVNEVLDSHPVDQVILALPLAGHAHVKALMDKLALRTVDVRV
VPDLYQYITLYGGLEEFGGLPIIRLQGDPMEGWSRVAKRAFDILFSLLAILITAPLMAATALAVRLTSRGPMLYRQERMG
MDGRTFPILKFRTMCIDAEHGGAMMTCPDDPRRTVIGTFLRKYSLDELPQFFNVLRGDMSLVGPRPERPVFIEEFKRQIP
RYHLRHKVKAGITGWAQINGLRGQTCIEKRIEYDLYYIENWSLLMDLKILVRTALGGFLSKNAY
>Mature_464_residues
MFSRLQRFYTSIKVVADMVMLALAFVLAYATRFSGIVPVTEGIPPWTDSFFSLLMVLVIFPVTFHQSRLYGTNRSRTNTG
EVFEVFKSTITAALILVAVTYFVRERYSRLTLVIFIVYAFALVTCSRLVTRYLVSEIRRRGHNLKSILVIGAGDLGQRVI
ETVEHHRELGFRVMGVLTLHPEKVGQYVNDVRVIGVVDQVNEVLDSHPVDQVILALPLAGHAHVKALMDKLALRTVDVRV
VPDLYQYITLYGGLEEFGGLPIIRLQGDPMEGWSRVAKRAFDILFSLLAILITAPLMAATALAVRLTSRGPMLYRQERMG
MDGRTFPILKFRTMCIDAEHGGAMMTCPDDPRRTVIGTFLRKYSLDELPQFFNVLRGDMSLVGPRPERPVFIEEFKRQIP
RYHLRHKVKAGITGWAQINGLRGQTCIEKRIEYDLYYIENWSLLMDLKILVRTALGGFLSKNAY

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: COG2148

COG function: function code M; Sugar transferases involved in lipopolysaccharide synthesis

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial sugar transferase family [H]

Homologues:

Organism=Escherichia coli, GI1788360, Length=360, Percent_Identity=40.2777777777778, Blast_Score=265, Evalue=3e-72,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003362
- InterPro:   IPR017475
- InterPro:   IPR017473 [H]

Pfam domain/function: PF02397 Bac_transf [H]

EC number: NA

Molecular weight: Translated: 52697; Mature: 52697

Theoretical pI: Translated: 9.93; Mature: 9.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFSRLQRFYTSIKVVADMVMLALAFVLAYATRFSGIVPVTEGIPPWTDSFFSLLMVLVIF
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHH
PVTFHQSRLYGTNRSRTNTGEVFEVFKSTITAALILVAVTYFVRERYSRLTLVIFIVYAF
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALVTCSRLVTRYLVSEIRRRGHNLKSILVIGAGDLGQRVIETVEHHRELGFRVMGVLTLH
HHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHHCCHHHHHHEEEC
PEKVGQYVNDVRVIGVVDQVNEVLDSHPVDQVILALPLAGHAHVKALMDKLALRTVDVRV
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHEEE
VPDLYQYITLYGGLEEFGGLPIIRLQGDPMEGWSRVAKRAFDILFSLLAILITAPLMAAT
HHHHHHHHHHHCCHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALAVRLTSRGPMLYRQERMGMDGRTFPILKFRTMCIDAEHGGAMMTCPDDPRRTVIGTFL
HHHHHCCCCCCCHHHHHHCCCCCCCCHHHHHHHHEEECCCCCEEEECCCCCHHHHHHHHH
RKYSLDELPQFFNVLRGDMSLVGPRPERPVFIEEFKRQIPRYHLRHKVKAGITGWAQING
HHHCHHHHHHHHHHHHCCHHHCCCCCCCCHHHHHHHHHCCHHHHHHHHHCCCCCHHHCCC
LRGQTCIEKRIEYDLYYIENWSLLMDLKILVRTALGGFLSKNAY
CCCHHHHHHHHHHEEEEEECCHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MFSRLQRFYTSIKVVADMVMLALAFVLAYATRFSGIVPVTEGIPPWTDSFFSLLMVLVIF
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHH
PVTFHQSRLYGTNRSRTNTGEVFEVFKSTITAALILVAVTYFVRERYSRLTLVIFIVYAF
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALVTCSRLVTRYLVSEIRRRGHNLKSILVIGAGDLGQRVIETVEHHRELGFRVMGVLTLH
HHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHHCCHHHHHHEEEC
PEKVGQYVNDVRVIGVVDQVNEVLDSHPVDQVILALPLAGHAHVKALMDKLALRTVDVRV
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHEEE
VPDLYQYITLYGGLEEFGGLPIIRLQGDPMEGWSRVAKRAFDILFSLLAILITAPLMAAT
HHHHHHHHHHHCCHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALAVRLTSRGPMLYRQERMGMDGRTFPILKFRTMCIDAEHGGAMMTCPDDPRRTVIGTFL
HHHHHCCCCCCCHHHHHHCCCCCCCCHHHHHHHHEEECCCCCEEEECCCCCHHHHHHHHH
RKYSLDELPQFFNVLRGDMSLVGPRPERPVFIEEFKRQIPRYHLRHKVKAGITGWAQING
HHHCHHHHHHHHHHHHCCHHHCCCCCCCCHHHHHHHHHCCHHHHHHHHHCCCCCHHHCCC
LRGQTCIEKRIEYDLYYIENWSLLMDLKILVRTALGGFLSKNAY
CCCHHHHHHHHHHEEEEEECCHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8759852; 9097040; 9278503 [H]