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The map label for this gene is lgrC [H]

Identifier: 108758947

GI number: 108758947

Start: 5299998

End: 5301512

Strand: Direct

Name: lgrC [H]

Synonym: MXAN_4305

Alternate gene names: 108758947

Gene position: 5299998-5301512 (Clockwise)

Preceding gene: 108759280

Following gene: 108762178

Centisome position: 57.99

GC content: 68.78

Gene sequence:

>1515_bases
ATGACACTCGACCAAATCGTCATCCGTGCAGCGGCGAAGGCTCCCGAGTCCATCGCCATCAAGGGTCCCGACGGGACCCT
CACCTACGGCCAGTTGGACGCGCTCGCCAACCGCATCGCCCGCGCGCTCCAGGAGCTGGGCGTGAAGCAGGGAGACCGCG
TGGGGCTGTGGACGGAAAAGTCCGTGCGCGCCGTCGCCGCGATGCAGGGCATTGCCCGGCTGGGCGCCGCCTACGTCCCC
TTGGACCCGCTGAACCCCGCGACGCGAACGCGGCTCATCCTCGACGACTGCGGCATTGACGTGATGGTGACCACCACCAC
GCGCGCGTCCGAGCTCCACAACGCCGGCGTGAGCCGGCTGCGCTACCTGCTGGTGGACGACAAGGGGCCCGAGATCTGCT
GGAACCGGCTGTCCGGTTTTTCATCTGAGCCGCTGCCGCCGCACGGCGCGGGCGACCACGACCTGGCGTACATCCTCTAC
ACGTCAGGCTCCACGGGAACGCCCAAGGGCGTGTGCATCAGCCAGCGGAACGCGATGGCCTTCATCGAGTGGTGCCATGC
GCTCCTGGGCACCACACCGGAGGACCGCTTCAGCAACCACGCGCCGTTCTTCTTCGACCTCTCGGTGCTGGACCTGTATG
CGGCCTTCATGGGCGGCGCGTCCGTCACGCTCATCCCGGAGGCGCTGGCCTTCGCGCCGGAGAAGCTGGTGGAGCTGGTC
CTCCGCGAGCGGTTCACCTGTTGGTACTCGGTGCCGTCGGCGCTGATGCTGATGATGCAGGAAGGCGGCCTGCTGAAGCA
TGGGGCGCTGCCCTTCCGCGCCGTGCTCTTCGCCGGCGAGCCGTTCCCCATCCGGCACCTGCGCCCGCTTCGTGAGCACC
TGCCACAGGCGCGGTTCTTCAACCTCTATGGACCCACGGAGACCAACGTCTGCACCTTCCATGAGGTGACGGACATTTCG
CCCCACCGCACCGAGCCGGTGCCCATTGGCCGAGCGAGCTGTGGCAACCGCGTGTGGTTGGCCCGGCCGCCGCCGGGCTC
AGAGGAAGCGCGGGAGGAAGGCGACGGCGTGGGCGAGCTGATGGTGGAAGGCCCCACGGTGATGCTGGGCTACTGGGGCC
AGCCCCGCCATGGGAGCGGGCCGTATGCCACGGGGGACCGCTGCCGCGAGGTGCCGGACGGCACGTTCGAATACCTGGGC
CGCCGCGACAACATGCTGAAGGTGCGCGGGCGCCGCATCGAAGCGGGCGAAATCGAAGCGGCGCTGCTCACGCACCCGGA
CATCCGCGAGGCAGGCGTGATTGCCACCGGCTCCGGCCTGGAAGCGCGGCTGGTGGCCTTCGTGGTCAGCGGCGCCAGCA
AGCCCCCTTCCCTGCTCAAGGTGAAGAAGCACTGCGCGGAGCGGTTGCCTCGCTACATGATTGTCGACGAGGTCCGCGTG
CTGCCCGAGCTGCCCCGGACTCCCAACGGCAAGCTCAACCGGCGCGCGCTCAGGGAACTGACGCAGGCGCCTTGA

Upstream 100 bases:

>100_bases
AAACGGGCATCGAGCGCGTGCTGCGTGACGCCATTCCCAGCGCCATCTTCTCCGGCACCTCCGAAATCCAACGCGACATC
ATCGCCAACCAGCTCGGCCT

Downstream 100 bases:

>100_bases
GCCGGTGACGGGCCGCCAGGGAGCCTGGCGGCCCTCCCCACACCGTCCGTGCAACCGCCCCATGCCTGCCCGCCTGCCCA
CAACGTCTCCTCGACTTCGT

Product: amino acid adenyltransferase

Products: pyrophosphate; AMP; enterobactin; pyrophosphate; L-Seryl-AMP [C]

Alternate protein names: ATP-dependent valine adenylase; ValA; Valine activase; ATP-dependent D-valine adenylase; D-ValA; D-valine activase; Valine racemase [ATP-hydrolyzing]; ATP-dependent tryptophan adenylase; TrpA; Tryptophan activase; ATP-dependent D-leucine adenylase; D-LeuA; D-leucine activase; Leucine racemase [ATP-hydrolyzing]; ATP-dependent tryptophan/phenylalanine/tyrosine adenylase; Trp/Phe/TyrA; Tryptophan/phenylalanine/tyrosine activase; ATP-dependent D-leucine adenylase; D-LeuA; D-leucine activase; Leucine racemase [ATP-hydrolyzing] [H]

Number of amino acids: Translated: 504; Mature: 503

Protein sequence:

>504_residues
MTLDQIVIRAAAKAPESIAIKGPDGTLTYGQLDALANRIARALQELGVKQGDRVGLWTEKSVRAVAAMQGIARLGAAYVP
LDPLNPATRTRLILDDCGIDVMVTTTTRASELHNAGVSRLRYLLVDDKGPEICWNRLSGFSSEPLPPHGAGDHDLAYILY
TSGSTGTPKGVCISQRNAMAFIEWCHALLGTTPEDRFSNHAPFFFDLSVLDLYAAFMGGASVTLIPEALAFAPEKLVELV
LRERFTCWYSVPSALMLMMQEGGLLKHGALPFRAVLFAGEPFPIRHLRPLREHLPQARFFNLYGPTETNVCTFHEVTDIS
PHRTEPVPIGRASCGNRVWLARPPPGSEEAREEGDGVGELMVEGPTVMLGYWGQPRHGSGPYATGDRCREVPDGTFEYLG
RRDNMLKVRGRRIEAGEIEAALLTHPDIREAGVIATGSGLEARLVAFVVSGASKPPSLLKVKKHCAERLPRYMIVDEVRV
LPELPRTPNGKLNRRALRELTQAP

Sequences:

>Translated_504_residues
MTLDQIVIRAAAKAPESIAIKGPDGTLTYGQLDALANRIARALQELGVKQGDRVGLWTEKSVRAVAAMQGIARLGAAYVP
LDPLNPATRTRLILDDCGIDVMVTTTTRASELHNAGVSRLRYLLVDDKGPEICWNRLSGFSSEPLPPHGAGDHDLAYILY
TSGSTGTPKGVCISQRNAMAFIEWCHALLGTTPEDRFSNHAPFFFDLSVLDLYAAFMGGASVTLIPEALAFAPEKLVELV
LRERFTCWYSVPSALMLMMQEGGLLKHGALPFRAVLFAGEPFPIRHLRPLREHLPQARFFNLYGPTETNVCTFHEVTDIS
PHRTEPVPIGRASCGNRVWLARPPPGSEEAREEGDGVGELMVEGPTVMLGYWGQPRHGSGPYATGDRCREVPDGTFEYLG
RRDNMLKVRGRRIEAGEIEAALLTHPDIREAGVIATGSGLEARLVAFVVSGASKPPSLLKVKKHCAERLPRYMIVDEVRV
LPELPRTPNGKLNRRALRELTQAP
>Mature_503_residues
TLDQIVIRAAAKAPESIAIKGPDGTLTYGQLDALANRIARALQELGVKQGDRVGLWTEKSVRAVAAMQGIARLGAAYVPL
DPLNPATRTRLILDDCGIDVMVTTTTRASELHNAGVSRLRYLLVDDKGPEICWNRLSGFSSEPLPPHGAGDHDLAYILYT
SGSTGTPKGVCISQRNAMAFIEWCHALLGTTPEDRFSNHAPFFFDLSVLDLYAAFMGGASVTLIPEALAFAPEKLVELVL
RERFTCWYSVPSALMLMMQEGGLLKHGALPFRAVLFAGEPFPIRHLRPLREHLPQARFFNLYGPTETNVCTFHEVTDISP
HRTEPVPIGRASCGNRVWLARPPPGSEEAREEGDGVGELMVEGPTVMLGYWGQPRHGSGPYATGDRCREVPDGTFEYLGR
RDNMLKVRGRRIEAGEIEAALLTHPDIREAGVIATGSGLEARLVAFVVSGASKPPSLLKVKKHCAERLPRYMIVDEVRVL
PELPRTPNGKLNRRALRELTQAP

Specific function: Activates the 7th to 12th amino acids (Val, D-Val, Trp, D-Leu, Xaa and D-Leu) in linear gramicidin and catalyzes the formation of the peptide bond between them. This enzyme is also responsible for the epimerization of the 8th (D-Val), the 10th (D- Leu) an

COG id: COG1020

COG function: function code Q; Non-ribosomal peptide synthetase modules and related proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 6 acyl carrier domains [H]

Homologues:

Organism=Homo sapiens, GI45580730, Length=550, Percent_Identity=24.1818181818182, Blast_Score=118, Evalue=1e-26,
Organism=Homo sapiens, GI156151445, Length=546, Percent_Identity=24.1758241758242, Blast_Score=117, Evalue=3e-26,
Organism=Homo sapiens, GI28416953, Length=552, Percent_Identity=25.9057971014493, Blast_Score=115, Evalue=1e-25,
Organism=Homo sapiens, GI38505220, Length=359, Percent_Identity=25.6267409470752, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI42544132, Length=537, Percent_Identity=22.3463687150838, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI122937307, Length=388, Percent_Identity=24.7422680412371, Blast_Score=73, Evalue=5e-13,
Organism=Homo sapiens, GI8923896, Length=127, Percent_Identity=34.6456692913386, Blast_Score=72, Evalue=2e-12,
Organism=Homo sapiens, GI253970489, Length=127, Percent_Identity=34.6456692913386, Blast_Score=72, Evalue=2e-12,
Organism=Escherichia coli, GI1786801, Length=533, Percent_Identity=30.7692307692308, Blast_Score=176, Evalue=3e-45,
Organism=Escherichia coli, GI145693145, Length=530, Percent_Identity=27.3584905660377, Blast_Score=119, Evalue=7e-28,
Organism=Escherichia coli, GI1788107, Length=544, Percent_Identity=24.6323529411765, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1790505, Length=552, Percent_Identity=24.8188405797101, Blast_Score=105, Evalue=6e-24,
Organism=Escherichia coli, GI221142682, Length=509, Percent_Identity=23.5756385068762, Blast_Score=89, Evalue=9e-19,
Organism=Escherichia coli, GI1786810, Length=542, Percent_Identity=23.6162361623616, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1789201, Length=366, Percent_Identity=27.5956284153005, Blast_Score=69, Evalue=9e-13,
Organism=Caenorhabditis elegans, GI17531443, Length=529, Percent_Identity=24.3856332703214, Blast_Score=109, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI17557194, Length=531, Percent_Identity=22.4105461393597, Blast_Score=91, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17559526, Length=392, Percent_Identity=23.7244897959184, Blast_Score=90, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI32564422, Length=349, Percent_Identity=25.5014326647564, Blast_Score=89, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI32564420, Length=349, Percent_Identity=25.5014326647564, Blast_Score=89, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17556356, Length=463, Percent_Identity=25.9179265658747, Blast_Score=89, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI71983001, Length=546, Percent_Identity=23.992673992674, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI71982997, Length=546, Percent_Identity=23.992673992674, Blast_Score=74, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6319591, Length=567, Percent_Identity=22.0458553791887, Blast_Score=102, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6319264, Length=559, Percent_Identity=24.865831842576, Blast_Score=93, Evalue=9e-20,
Organism=Saccharomyces cerevisiae, GI6319699, Length=382, Percent_Identity=25.6544502617801, Blast_Score=80, Evalue=5e-16,
Organism=Drosophila melanogaster, GI24648676, Length=525, Percent_Identity=26.8571428571429, Blast_Score=124, Evalue=2e-28,
Organism=Drosophila melanogaster, GI21355181, Length=496, Percent_Identity=24.1935483870968, Blast_Score=105, Evalue=6e-23,
Organism=Drosophila melanogaster, GI62472339, Length=550, Percent_Identity=22.3636363636364, Blast_Score=102, Evalue=5e-22,
Organism=Drosophila melanogaster, GI24667955, Length=573, Percent_Identity=22.3385689354276, Blast_Score=102, Evalue=6e-22,
Organism=Drosophila melanogaster, GI24648260, Length=524, Percent_Identity=24.4274809160305, Blast_Score=99, Evalue=6e-21,
Organism=Drosophila melanogaster, GI18859661, Length=380, Percent_Identity=25.7894736842105, Blast_Score=98, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24667959, Length=394, Percent_Identity=23.0964467005076, Blast_Score=89, Evalue=6e-18,
Organism=Drosophila melanogaster, GI21356441, Length=536, Percent_Identity=24.6268656716418, Blast_Score=84, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010071
- InterPro:   IPR009081
- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR023213
- InterPro:   IPR001242
- InterPro:   IPR010060
- InterPro:   IPR006163
- InterPro:   IPR020806
- InterPro:   IPR006162 [H]

Pfam domain/function: PF00501 AMP-binding; PF00668 Condensation; PF00550 PP-binding [H]

EC number: 2.7.7.- [C]

Molecular weight: Translated: 55191; Mature: 55060

Theoretical pI: Translated: 7.56; Mature: 7.56

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLDQIVIRAAAKAPESIAIKGPDGTLTYGQLDALANRIARALQELGVKQGDRVGLWTEK
CCHHHHHHHHHHCCCCCEEEECCCCCEEHHHHHHHHHHHHHHHHHCCCCCCCEEEECCHH
SVRAVAAMQGIARLGAAYVPLDPLNPATRTRLILDDCGIDVMVTTTTRASELHNAGVSRL
HHHHHHHHHHHHHHCCCCCCCCCCCHHHHEEEEEECCCCEEEEEECCHHHHHHHHHHHHE
RYLLVDDKGPEICWNRLSGFSSEPLPPHGAGDHDLAYILYTSGSTGTPKGVCISQRNAMA
EEEEEECCCHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCEEEECCCHHH
FIEWCHALLGTTPEDRFSNHAPFFFDLSVLDLYAAFMGGASVTLIPEALAFAPEKLVELV
HHHHHHHHHCCCCHHHCCCCCCEEEEHHHHHHHHHHHCCCCEEEEHHHHHHCHHHHHHHH
LRERFTCWYSVPSALMLMMQEGGLLKHGALPFRAVLFAGEPFPIRHLRPLREHLPQARFF
HHHHHHHHHHHHHHHHHHHHCCCCEECCCCCEEEEEECCCCCCHHHHHHHHHHCCCCEEE
NLYGPTETNVCTFHEVTDISPHRTEPVPIGRASCGNRVWLARPPPGSEEAREEGDGVGEL
EECCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHCCCCEEE
MVEGPTVMLGYWGQPRHGSGPYATGDRCREVPDGTFEYLGRRDNMLKVRGRRIEAGEIEA
EECCCEEEEECCCCCCCCCCCCCCCHHHHCCCCCHHHHHCCCCCEEEECCCEEECCCCCE
ALLTHPDIREAGVIATGSGLEARLVAFVVSGASKPPSLLKVKKHCAERLPRYMIVDEVRV
EEECCCCCHHCCEEEECCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCEEHHHHHHH
LPELPRTPNGKLNRRALRELTQAP
HHCCCCCCCCCCHHHHHHHHHCCC
>Mature Secondary Structure 
TLDQIVIRAAAKAPESIAIKGPDGTLTYGQLDALANRIARALQELGVKQGDRVGLWTEK
CHHHHHHHHHHCCCCCEEEECCCCCEEHHHHHHHHHHHHHHHHHCCCCCCCEEEECCHH
SVRAVAAMQGIARLGAAYVPLDPLNPATRTRLILDDCGIDVMVTTTTRASELHNAGVSRL
HHHHHHHHHHHHHHCCCCCCCCCCCHHHHEEEEEECCCCEEEEEECCHHHHHHHHHHHHE
RYLLVDDKGPEICWNRLSGFSSEPLPPHGAGDHDLAYILYTSGSTGTPKGVCISQRNAMA
EEEEEECCCHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCEEEECCCHHH
FIEWCHALLGTTPEDRFSNHAPFFFDLSVLDLYAAFMGGASVTLIPEALAFAPEKLVELV
HHHHHHHHHCCCCHHHCCCCCCEEEEHHHHHHHHHHHCCCCEEEEHHHHHHCHHHHHHHH
LRERFTCWYSVPSALMLMMQEGGLLKHGALPFRAVLFAGEPFPIRHLRPLREHLPQARFF
HHHHHHHHHHHHHHHHHHHHCCCCEECCCCCEEEEEECCCCCCHHHHHHHHHHCCCCEEE
NLYGPTETNVCTFHEVTDISPHRTEPVPIGRASCGNRVWLARPPPGSEEAREEGDGVGEL
EECCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHCCCCEEE
MVEGPTVMLGYWGQPRHGSGPYATGDRCREVPDGTFEYLGRRDNMLKVRGRRIEAGEIEA
EECCCEEEEECCCCCCCCCCCCCCCHHHHCCCCCHHHHHCCCCCEEEECCCEEECCCCCE
ALLTHPDIREAGVIATGSGLEARLVAFVVSGASKPPSLLKVKKHCAERLPRYMIVDEVRV
EEECCCCCHHCCEEEECCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCEEHHHHHHH
LPELPRTPNGKLNRRALRELTQAP
HHCCCCCCCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Phosphopantetheine. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 6 ATP; L-serine; 2,3-dihydroxybenzoate [C]

Specific reaction: 6 ATP + 3 L-serine + 3 2,3-dihydroxybenzoate = 6 pyrophosphate + 6 AMP + enterobactin ATP + L-serine = pyrophosphate + L-Seryl-AMP 6 ATP + 3 L-serine + 3 2,3-dihydroxybenzoate = 6 pyrophosphate + 6 AMP + enterobactin ATP + L-serine = pyrophosphate + L-Ser

General reaction: Transferases; Acyltransferases; Transferring groups other than amino-acyl groups [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA