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The map label for this gene is tilS [H]

Identifier: 108758860

GI number: 108758860

Start: 5366031

End: 5367386

Strand: Reverse

Name: tilS [H]

Synonym: MXAN_4360

Alternate gene names: 108758860

Gene position: 5367386-5366031 (Counterclockwise)

Preceding gene: 108760562

Following gene: 108759619

Centisome position: 58.73

GC content: 74.04

Gene sequence:

>1356_bases
ATGCCCCGTCACGATTCCGCCACGCAGCTGCTGACGACGACGCTTCAAGAGGCGTACACCCGGCTGGGACTCGAGGGAGG
TTCGGTGCTGCTCGCCGTTTCCGGCGGCGCGGACTCCACGGCCCTCCTGATGGGAACCGTGCTCGTCGCCGAGCGACTCC
GGCTTCGCGTGGAGGTGGCCTCGCTGGACCATGGCCTGCGTCCCGAAGCGGCGGAGGAGGCCCGCCAGGTGTCGGTCCTG
GCGGCCGCGCGGGGGCTGCCCTGCCACGTCCAGGCGCTGCGGCTGCATCCAGGGGTCGGCATCGAGGCGCGCGCGAGGGA
GGCTCGCTACGCGGCGCTGGAGGCACTCCGCCTCGAGCGGGGCCTGGACGTGGTGGCCACCGCGCACACCGCCACGGACC
AGGCGGAGACGCTGCTCATGCGCCTGGCACGAGGCACCGCCCTGCGCGGCGCGGTGGGCATCCACGAAGCGCGCCCCGGA
CTGGTGCGCCCCCTGCTGTCGTGCACGCGGGAGGAGGTGGTGGCCTTCCTGGCGGAGCAGGGTGTCGCCTATGTGACAGA
CCCGATGAACACCGACCCTGTCCACTTCCGGACGCGCGTTCGCCTTGGAGTGCTACCCGCCCTGTCGCGCGCCGCTGGCT
TCGCGGTGGAGGGGCACCTGGCCGCCTTCGCGCGGGTGGTCGCGGAGGACGAGTCGCTGCTGGCGTCCATGGCGGACGCG
GCCTTCGACCGGCTGCGCCTGGAGGATGGCGCCCTGGATGCGGTGGGCGTGCGCGCGCTGGAGCCCGCGCTGCGCCGGCG
GGTGCTCGCCCGGCTGGTGGCCGGCACGGAGGCCGCGGTGGATGAGGCCACGCTGGCCCGCGTGCAGCGCGCGGTGGCCC
AGGGCGGCACGGCCACGCTGGGGCGGGGCTACGTGCTGCGCGCGACCAGCGGACGGGTGCGCTGCGTGCGGCAGGTGCCG
TCCACCCCACCGGCGGAACTCCGGCTGGAGCACGCGGGTTCGCGGGGCGCGCTCGCGGGGACGGGCTGGAATTTTTCCGT
CGAGTTCGCACCTCCACCAGCGGGCGTGCTCGGGCTGGCGCTCGGGGACGGGACGCGCTGGCCGCTGACCGTGCGGACAC
GCCGCCCCGGCGATCGGGTGTGCACTGCTGGGGGACAACGCAAGCTTCAAGATGTGTTGGTTGATCTTCGAGTGCCCGCG
GAAGCACGGGCCACGCGGCCGGTGGTGGCGGATGCCGAGGGGCAGGTGTTGTGGCTCCCCGGCCTCTGGTCTCCGGTGGC
TCCGCGTGCGGCCGCCAGGGAGTACCTCTGGGCGGTCCCTCCCGGTTCGAGCATTCAGCGGACCGCGGCGTTATAG

Upstream 100 bases:

>100_bases
TGATTCGATGTTGTGGCGATCATAAGAATCAGCCCCCCTGCCTTCAACCGCACGTGCCTCTGTAACCCGCTCACCCGACA
CTCCATTCCACCGCGGTCCG

Downstream 100 bases:

>100_bases
AGTGGCAGAGTCGCAGTTCGGGGGATGGGCGCGGCCCCCTAAATAGTTGAGGGCTTTTTGCTGTTGCTGATACGGTCCGT
CGCTGGGTAGCTCGCCTGGT

Product: tRNA(Ile)-lysidine synthetase

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase [H]

Number of amino acids: Translated: 451; Mature: 450

Protein sequence:

>451_residues
MPRHDSATQLLTTTLQEAYTRLGLEGGSVLLAVSGGADSTALLMGTVLVAERLRLRVEVASLDHGLRPEAAEEARQVSVL
AAARGLPCHVQALRLHPGVGIEARAREARYAALEALRLERGLDVVATAHTATDQAETLLMRLARGTALRGAVGIHEARPG
LVRPLLSCTREEVVAFLAEQGVAYVTDPMNTDPVHFRTRVRLGVLPALSRAAGFAVEGHLAAFARVVAEDESLLASMADA
AFDRLRLEDGALDAVGVRALEPALRRRVLARLVAGTEAAVDEATLARVQRAVAQGGTATLGRGYVLRATSGRVRCVRQVP
STPPAELRLEHAGSRGALAGTGWNFSVEFAPPPAGVLGLALGDGTRWPLTVRTRRPGDRVCTAGGQRKLQDVLVDLRVPA
EARATRPVVADAEGQVLWLPGLWSPVAPRAAAREYLWAVPPGSSIQRTAAL

Sequences:

>Translated_451_residues
MPRHDSATQLLTTTLQEAYTRLGLEGGSVLLAVSGGADSTALLMGTVLVAERLRLRVEVASLDHGLRPEAAEEARQVSVL
AAARGLPCHVQALRLHPGVGIEARAREARYAALEALRLERGLDVVATAHTATDQAETLLMRLARGTALRGAVGIHEARPG
LVRPLLSCTREEVVAFLAEQGVAYVTDPMNTDPVHFRTRVRLGVLPALSRAAGFAVEGHLAAFARVVAEDESLLASMADA
AFDRLRLEDGALDAVGVRALEPALRRRVLARLVAGTEAAVDEATLARVQRAVAQGGTATLGRGYVLRATSGRVRCVRQVP
STPPAELRLEHAGSRGALAGTGWNFSVEFAPPPAGVLGLALGDGTRWPLTVRTRRPGDRVCTAGGQRKLQDVLVDLRVPA
EARATRPVVADAEGQVLWLPGLWSPVAPRAAAREYLWAVPPGSSIQRTAAL
>Mature_450_residues
PRHDSATQLLTTTLQEAYTRLGLEGGSVLLAVSGGADSTALLMGTVLVAERLRLRVEVASLDHGLRPEAAEEARQVSVLA
AARGLPCHVQALRLHPGVGIEARAREARYAALEALRLERGLDVVATAHTATDQAETLLMRLARGTALRGAVGIHEARPGL
VRPLLSCTREEVVAFLAEQGVAYVTDPMNTDPVHFRTRVRLGVLPALSRAAGFAVEGHLAAFARVVAEDESLLASMADAA
FDRLRLEDGALDAVGVRALEPALRRRVLARLVAGTEAAVDEATLARVQRAVAQGGTATLGRGYVLRATSGRVRCVRQVPS
TPPAELRLEHAGSRGALAGTGWNFSVEFAPPPAGVLGLALGDGTRWPLTVRTRRPGDRVCTAGGQRKLQDVLVDLRVPAE
ARATRPVVADAEGQVLWLPGLWSPVAPRAAAREYLWAVPPGSSIQRTAAL

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786386, Length=403, Percent_Identity=28.7841191066998, Blast_Score=94, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012094
- InterPro:   IPR012796
- InterPro:   IPR012795
- InterPro:   IPR015262
- InterPro:   IPR020825
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3; PF09179 DUF1946; PF11734 TilS_C [H]

EC number: NA

Molecular weight: Translated: 47768; Mature: 47637

Theoretical pI: Translated: 9.91; Mature: 9.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRHDSATQLLTTTLQEAYTRLGLEGGSVLLAVSGGADSTALLMGTVLVAERLRLRVEVA
CCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHEEEEE
SLDHGLRPEAAEEARQVSVLAAARGLPCHVQALRLHPGVGIEARAREARYAALEALRLER
CCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHC
GLDVVATAHTATDQAETLLMRLARGTALRGAVGIHEARPGLVRPLLSCTREEVVAFLAEQ
CCCEEEECCCCCHHHHHHHHHHHCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHC
GVAYVTDPMNTDPVHFRTRVRLGVLPALSRAAGFAVEGHLAAFARVVAEDESLLASMADA
CCEEEECCCCCCCCEEEEEEEHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHHHHH
AFDRLRLEDGALDAVGVRALEPALRRRVLARLVAGTEAAVDEATLARVQRAVAQGGTATL
HHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCEEC
GRGYVLRATSGRVRCVRQVPSTPPAELRLEHAGSRGALAGTGWNFSVEFAPPPAGVLGLA
CCCEEEEECCCCHHHHCCCCCCCCHHEEEECCCCCCCEEECCCEEEEEECCCCCCEEEEE
LGDGTRWPLTVRTRRPGDRVCTAGGQRKLQDVLVDLRVPAEARATRPVVADAEGQVLWLP
ECCCCCCCEEEECCCCCCCEECCCCHHHHHHHHHHHCCCCHHHCCCCEEECCCCCEEECC
GLWSPVAPRAAAREYLWAVPPGSSIQRTAAL
CCCCCCCCHHHHHHCEEECCCCCCCHHHHCC
>Mature Secondary Structure 
PRHDSATQLLTTTLQEAYTRLGLEGGSVLLAVSGGADSTALLMGTVLVAERLRLRVEVA
CCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHEEEEE
SLDHGLRPEAAEEARQVSVLAAARGLPCHVQALRLHPGVGIEARAREARYAALEALRLER
CCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHC
GLDVVATAHTATDQAETLLMRLARGTALRGAVGIHEARPGLVRPLLSCTREEVVAFLAEQ
CCCEEEECCCCCHHHHHHHHHHHCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHC
GVAYVTDPMNTDPVHFRTRVRLGVLPALSRAAGFAVEGHLAAFARVVAEDESLLASMADA
CCEEEECCCCCCCCEEEEEEEHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHHHHH
AFDRLRLEDGALDAVGVRALEPALRRRVLARLVAGTEAAVDEATLARVQRAVAQGGTATL
HHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCEEC
GRGYVLRATSGRVRCVRQVPSTPPAELRLEHAGSRGALAGTGWNFSVEFAPPPAGVLGLA
CCCEEEEECCCCHHHHCCCCCCCCHHEEEECCCCCCCEEECCCEEEEEECCCCCCEEEEE
LGDGTRWPLTVRTRRPGDRVCTAGGQRKLQDVLVDLRVPAEARATRPVVADAEGQVLWLP
ECCCCCCCEEEECCCCCCCEECCCCHHHHHHHHHHHCCCCHHHCCCCEEECCCCCEEECC
GLWSPVAPRAAAREYLWAVPPGSSIQRTAAL
CCCCCCCCHHHHHHCEEECCCCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]