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The map label for this gene is ksgA

Identifier: 108758752

GI number: 108758752

Start: 2384738

End: 2385589

Strand: Direct

Name: ksgA

Synonym: MXAN_2052

Alternate gene names: 108758752

Gene position: 2384738-2385589 (Clockwise)

Preceding gene: 108762785

Following gene: 108757889

Centisome position: 26.09

GC content: 71.24

Gene sequence:

>852_bases
GTGGAATCGCCTAGAGACATCCTCAAGCGGCATGGCCTGCGCGCCAAGTACAGCTGGGGACAGAACTTCCTCGGAGACGA
GGACGCGCTGGAGGCCATCGCCGACGCGCTGAACCTGCGCGCCGATGAGCCGGTGGTGGAGCTGGGCCCGGGCCTGGGCC
ACCTCACGCGCTTCCTGGCCGCCACCGGGGCCCGCGTCACCGCCGTGGAGCGGGACCGGGACATGGTGATGGTGCTGGAG
AAGGAGGCCATCCCCGGCGTGCGCGTGGTGTCCGGCAACGCGGCCACGGTGGACTTCGCCCAGGTGGCTGGCGCGCCCGA
CGTCGCGGTGGCGGGAAACCTCCCGTACCACCTCACCAGCCCCATTCTCTTCCGAGTGCTGGAGCAGCGGGCCCATGTCT
CGCGCGCGGTCTTCACGCTCCAGAAGGAAGTGGTGGAGCGGCTCGCCGCGGAGCCCGGCAACCGGGACTACGGTCTGCTG
ACGGTGCTGCTGGGCATGCACTACGACGCGGAGAACGTCCTCACGCTGGAGGCGTGGCGCTTCCATCCGCCCCCGAAGGT
GGACTCCGCGGTGCTGCGGCTCACCCGCCGCAAGTCGCCGCGCGCGCCCATCATCGACGAGGCCCGCTTCACCCGCGTGG
TGAAGGCGTCCTTCGCGCACCGGCGCAAGACGCTCATCAACTCCATCAAGTCGGACCCGACGCTCGGGACGACGGAGACG
CTCATCGCCGCCCTGGAGGCCGCGGGGGTGGATCCGCAGCGCCGCGCGGAGACGCTGACTCCCGAGGAGTTCGCCGCCAT
CGAGCGCGCGCTCGGCCCCCTGACGCCGGCTACGTCCACGCCCGCGGAGTAG

Upstream 100 bases:

>100_bases
TGATTGCGGTGGCGGGGTATGAGGCGTACCGCCGCGGCCTGCGCGGAGATTTCCGCCTCGCCGCCGACCCGGCCTGGCGC
ATGTAGAGGGGAGCAGACGG

Downstream 100 bases:

>100_bases
GGCGCCGCTAGCGTCCGCGCATGCCCAGTGCCTGGAGCAGGCCGCCTTGCTGCTCCAGGTAGGCGAAGAACTCGGCTTCG
GGGATGCGCATCCGGGCGAG

Product: dimethyladenosine transferase

Products: NA

Alternate protein names: 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase; 16S rRNA dimethyladenosine transferase; 16S rRNA dimethylase; S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase

Number of amino acids: Translated: 283; Mature: 283

Protein sequence:

>283_residues
MESPRDILKRHGLRAKYSWGQNFLGDEDALEAIADALNLRADEPVVELGPGLGHLTRFLAATGARVTAVERDRDMVMVLE
KEAIPGVRVVSGNAATVDFAQVAGAPDVAVAGNLPYHLTSPILFRVLEQRAHVSRAVFTLQKEVVERLAAEPGNRDYGLL
TVLLGMHYDAENVLTLEAWRFHPPPKVDSAVLRLTRRKSPRAPIIDEARFTRVVKASFAHRRKTLINSIKSDPTLGTTET
LIAALEAAGVDPQRRAETLTPEEFAAIERALGPLTPATSTPAE

Sequences:

>Translated_283_residues
MESPRDILKRHGLRAKYSWGQNFLGDEDALEAIADALNLRADEPVVELGPGLGHLTRFLAATGARVTAVERDRDMVMVLE
KEAIPGVRVVSGNAATVDFAQVAGAPDVAVAGNLPYHLTSPILFRVLEQRAHVSRAVFTLQKEVVERLAAEPGNRDYGLL
TVLLGMHYDAENVLTLEAWRFHPPPKVDSAVLRLTRRKSPRAPIIDEARFTRVVKASFAHRRKTLINSIKSDPTLGTTET
LIAALEAAGVDPQRRAETLTPEEFAAIERALGPLTPATSTPAE
>Mature_283_residues
MESPRDILKRHGLRAKYSWGQNFLGDEDALEAIADALNLRADEPVVELGPGLGHLTRFLAATGARVTAVERDRDMVMVLE
KEAIPGVRVVSGNAATVDFAQVAGAPDVAVAGNLPYHLTSPILFRVLEQRAHVSRAVFTLQKEVVERLAAEPGNRDYGLL
TVLLGMHYDAENVLTLEAWRFHPPPKVDSAVLRLTRRKSPRAPIIDEARFTRVVKASFAHRRKTLINSIKSDPTLGTTET
LIAALEAAGVDPQRRAETLTPEEFAAIERALGPLTPATSTPAE

Specific function: Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits

COG id: COG0030

COG function: function code J; Dimethyladenosine transferase (rRNA methylation)

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily

Homologues:

Organism=Homo sapiens, GI7657198, Length=225, Percent_Identity=30.6666666666667, Blast_Score=101, Evalue=8e-22,
Organism=Homo sapiens, GI156415992, Length=289, Percent_Identity=28.3737024221453, Blast_Score=92, Evalue=7e-19,
Organism=Escherichia coli, GI1786236, Length=271, Percent_Identity=33.210332103321, Blast_Score=148, Evalue=4e-37,
Organism=Caenorhabditis elegans, GI25146882, Length=212, Percent_Identity=31.6037735849057, Blast_Score=104, Evalue=4e-23,
Organism=Saccharomyces cerevisiae, GI6324989, Length=225, Percent_Identity=27.1111111111111, Blast_Score=86, Evalue=7e-18,
Organism=Drosophila melanogaster, GI21358017, Length=239, Percent_Identity=28.4518828451883, Blast_Score=99, Evalue=4e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMA_MYXXD (Q1DAP2)

Other databases:

- EMBL:   CP000113
- RefSeq:   YP_630281.1
- ProteinModelPortal:   Q1DAP2
- SMR:   Q1DAP2
- STRING:   Q1DAP2
- GeneID:   4101271
- GenomeReviews:   CP000113_GR
- KEGG:   mxa:MXAN_2052
- TIGR:   MXAN_2052
- eggNOG:   COG0030
- HOGENOM:   HBG319664
- OMA:   TPIIMKL
- PhylomeDB:   Q1DAP2
- ProtClustDB:   PRK00274
- BioCyc:   MXAN246197:MXAN_2052-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00607
- InterPro:   IPR023165
- InterPro:   IPR020596
- InterPro:   IPR001737
- InterPro:   IPR020598
- InterPro:   IPR011530
- Gene3D:   G3DSA:1.10.8.100
- PANTHER:   PTHR11727
- SMART:   SM00650
- TIGRFAMs:   TIGR00755

Pfam domain/function: PF00398 RrnaAD

EC number: =2.1.1.182

Molecular weight: Translated: 30872; Mature: 30872

Theoretical pI: Translated: 6.91; Mature: 6.91

Prosite motif: PS01131 RRNA_A_DIMETH

Important sites: BINDING 22-22 BINDING 24-24 BINDING 49-49 BINDING 70-70 BINDING 113-113

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MESPRDILKRHGLRAKYSWGQNFLGDEDALEAIADALNLRADEPVVELGPGLGHLTRFLA
CCCHHHHHHHCCCCEEECCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHCCCHHHHHHHHH
ATGARVTAVERDRDMVMVLEKEAIPGVRVVSGNAATVDFAQVAGAPDVAVAGNLPYHLTS
HCCCEEEEEECCCCEEEEEECCCCCCEEEECCCCCEEHHHHHCCCCCEEEECCCCHHHHH
PILFRVLEQRAHVSRAVFTLQKEVVERLAAEPGNRDYGLLTVLLGMHYDAENVLTLEAWR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCEEEEEEEE
FHPPPKVDSAVLRLTRRKSPRAPIIDEARFTRVVKASFAHRRKTLINSIKSDPTLGTTET
CCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
LIAALEAAGVDPQRRAETLTPEEFAAIERALGPLTPATSTPAE
HHHHHHHCCCCHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MESPRDILKRHGLRAKYSWGQNFLGDEDALEAIADALNLRADEPVVELGPGLGHLTRFLA
CCCHHHHHHHCCCCEEECCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHCCCHHHHHHHHH
ATGARVTAVERDRDMVMVLEKEAIPGVRVVSGNAATVDFAQVAGAPDVAVAGNLPYHLTS
HCCCEEEEEECCCCEEEEEECCCCCCEEEECCCCCEEHHHHHCCCCCEEEECCCCHHHHH
PILFRVLEQRAHVSRAVFTLQKEVVERLAAEPGNRDYGLLTVLLGMHYDAENVLTLEAWR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCEEEEEEEE
FHPPPKVDSAVLRLTRRKSPRAPIIDEARFTRVVKASFAHRRKTLINSIKSDPTLGTTET
CCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
LIAALEAAGVDPQRRAETLTPEEFAAIERALGPLTPATSTPAE
HHHHHHHCCCCHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA