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The map label for this gene is truA

Identifier: 108758714

GI number: 108758714

Start: 6389878

End: 6390675

Strand: Reverse

Name: truA

Synonym: MXAN_5115

Alternate gene names: 108758714

Gene position: 6390675-6389878 (Counterclockwise)

Preceding gene: 108759758

Following gene: 108760698

Centisome position: 69.92

GC content: 70.93

Gene sequence:

>798_bases
ATGCCCCGGCTGAAGCTGACGCTCGAATACGAAGGAACGCGCTACGTGGGCTGGCAGGTGCAGCCCAACGGGCCCTCCAT
CCAGTCGGTGCTGGAATCGGGACTGGAGCGGCTGCTGGGGGCGCGTGTGTCGGTGGCGTCGGCGGGGCGTACGGATGCGG
GCGTCCATGCCTCCGGGCAGGTGGCTTGCTTCGACTCGCCCCGGGTGTTGCCGATGAAGGCGTACGTCATGGGGCTCAAT
GGCCTGCTGCCTCCCGACGTGGCCGTGGTGGACGCGGTGGAGGTGGCCGAGGACTTCGACCCACGCCGGTGGTCCCGGGG
CAAGCGCTACCGGTATCGCGTGAGCAACCGGCGCACGCGCTCGCCCCTGCGGCGGATGACGCACTGGGAAGTCTTCGCTC
CGTTGGATGTGGAGGCCATGCGGCAGGCGGCCGCGCACCTGTTGGGGCGGCATGACTTCTCTGCCTTCAGGGCCGCGGAC
TGCCAGGCCAAGCACGCGGTGCGGGAGATACGGAGCCTGGCGGTGGAAGGCACGTCGGGGGACGCCGTGTCCTTCGTGGT
GGAGGGCACCGCGTTCCTGAAGCACATGGTGCGCAACCTGGCGGGGACATTGGTGGAGGTGGGGAAGGGACGGCGGCCCG
CGTCCTGGGTGGCGGAGGTGCTGGCCTCCCAGGAGCGCAAGCGCGCCGGGCCCACCGCGCCGCCTCAGGGGCTGGTGCTG
GAGGAGGTCTTCTACCGGGACGGCCCGCCTGCCCGCACGCCGGGGGGAACGACGGACGCGGAAGAGGATGAGGGCTGA

Upstream 100 bases:

>100_bases
CGTCTCGGAAGCGCTGGAGGCGGTGGCCCGCCGCAACAACGTGCCCGCGGGCCGCATGGTGGCCGAGCTGCTCGAGTCCT
GGCTCAAGTACCGCTGACCC

Downstream 100 bases:

>100_bases
AGTGGAGTAGGCTGCCTGCCCGTGAGCTCCAAGACGAGTGTCCTCGAACCGCTGCGCGTCCGTGTCCGTCGCTTCCAGTT
CATCATGGGACTGGGGTTCA

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I

Number of amino acids: Translated: 265; Mature: 264

Protein sequence:

>265_residues
MPRLKLTLEYEGTRYVGWQVQPNGPSIQSVLESGLERLLGARVSVASAGRTDAGVHASGQVACFDSPRVLPMKAYVMGLN
GLLPPDVAVVDAVEVAEDFDPRRWSRGKRYRYRVSNRRTRSPLRRMTHWEVFAPLDVEAMRQAAAHLLGRHDFSAFRAAD
CQAKHAVREIRSLAVEGTSGDAVSFVVEGTAFLKHMVRNLAGTLVEVGKGRRPASWVAEVLASQERKRAGPTAPPQGLVL
EEVFYRDGPPARTPGGTTDAEEDEG

Sequences:

>Translated_265_residues
MPRLKLTLEYEGTRYVGWQVQPNGPSIQSVLESGLERLLGARVSVASAGRTDAGVHASGQVACFDSPRVLPMKAYVMGLN
GLLPPDVAVVDAVEVAEDFDPRRWSRGKRYRYRVSNRRTRSPLRRMTHWEVFAPLDVEAMRQAAAHLLGRHDFSAFRAAD
CQAKHAVREIRSLAVEGTSGDAVSFVVEGTAFLKHMVRNLAGTLVEVGKGRRPASWVAEVLASQERKRAGPTAPPQGLVL
EEVFYRDGPPARTPGGTTDAEEDEG
>Mature_264_residues
PRLKLTLEYEGTRYVGWQVQPNGPSIQSVLESGLERLLGARVSVASAGRTDAGVHASGQVACFDSPRVLPMKAYVMGLNG
LLPPDVAVVDAVEVAEDFDPRRWSRGKRYRYRVSNRRTRSPLRRMTHWEVFAPLDVEAMRQAAAHLLGRHDFSAFRAADC
QAKHAVREIRSLAVEGTSGDAVSFVVEGTAFLKHMVRNLAGTLVEVGKGRRPASWVAEVLASQERKRAGPTAPPQGLVLE
EVFYRDGPPARTPGGTTDAEEDEG

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family

Homologues:

Organism=Homo sapiens, GI23503259, Length=274, Percent_Identity=32.4817518248175, Blast_Score=94, Evalue=2e-19,
Organism=Escherichia coli, GI1788657, Length=247, Percent_Identity=40.080971659919, Blast_Score=181, Evalue=6e-47,
Organism=Caenorhabditis elegans, GI71985046, Length=267, Percent_Identity=27.7153558052434, Blast_Score=78, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUA_MYXXA (Q93NE2)

Other databases:

- EMBL:   AF377337
- ProteinModelPortal:   Q93NE2
- SMR:   Q93NE2
- BRENDA:   5.4.99.12
- HAMAP:   MF_00171
- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094
- Gene3D:   G3DSA:3.30.70.580
- Gene3D:   G3DSA:3.30.70.660
- PANTHER:   PTHR11142
- PIRSF:   PIRSF001430
- TIGRFAMs:   TIGR00071

Pfam domain/function: PF01416 PseudoU_synth_1; SSF55120 PsdUridine_synth_cat_dom

EC number: =5.4.99.12

Molecular weight: Translated: 29014; Mature: 28883

Theoretical pI: Translated: 9.43; Mature: 9.43

Prosite motif: NA

Important sites: ACT_SITE 52-52

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRLKLTLEYEGTRYVGWQVQPNGPSIQSVLESGLERLLGARVSVASAGRTDAGVHASGQ
CCCEEEEEEECCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
VACFDSPRVLPMKAYVMGLNGLLPPDVAVVDAVEVAEDFDPRRWSRGKRYRYRVSNRRTR
EEEECCCCEEHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCHHHHCCCCEEEECCCCHHH
SPLRRMTHWEVFAPLDVEAMRQAAAHLLGRHDFSAFRAADCQAKHAVREIRSLAVEGTSG
HHHHHHHHHEEECCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCC
DAVSFVVEGTAFLKHMVRNLAGTLVEVGKGRRPASWVAEVLASQERKRAGPTAPPQGLVL
CEEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHH
EEVFYRDGPPARTPGGTTDAEEDEG
HHHHHCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
PRLKLTLEYEGTRYVGWQVQPNGPSIQSVLESGLERLLGARVSVASAGRTDAGVHASGQ
CCEEEEEEECCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
VACFDSPRVLPMKAYVMGLNGLLPPDVAVVDAVEVAEDFDPRRWSRGKRYRYRVSNRRTR
EEEECCCCEEHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCHHHHCCCCEEEECCCCHHH
SPLRRMTHWEVFAPLDVEAMRQAAAHLLGRHDFSAFRAADCQAKHAVREIRSLAVEGTSG
HHHHHHHHHEEECCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCC
DAVSFVVEGTAFLKHMVRNLAGTLVEVGKGRRPASWVAEVLASQERKRAGPTAPPQGLVL
CEEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHH
EEVFYRDGPPARTPGGTTDAEEDEG
HHHHHCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA