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The map label for this gene is tuf [H]

Identifier: 108758449

GI number: 108758449

Start: 3853234

End: 3854424

Strand: Direct

Name: tuf [H]

Synonym: MXAN_3298

Alternate gene names: 108758449

Gene position: 3853234-3854424 (Clockwise)

Preceding gene: 108757486

Following gene: 108763296

Centisome position: 42.16

GC content: 65.66

Gene sequence:

>1191_bases
ATGGCCAAGGAAAAGTTCGAGCGTAACAAGCCCCACGTGAACATCGGCACGATCGGACACGTGGACCACGGCAAGACGTC
GCTGACGGCCGCCATCACCAAGGTGCTGGCGAAGACGGGCGGCGCCACGTTCCTGGCGTACGACCTGATTGACAAGGCGC
CGGAGGAGCGTGAGCGCGGTATCACGATTTCCACCTCGCACGTGGAGTACCAGACGTCGAACCGGCACTACGCCCACGTC
GACTGTCCGGGCCACGCCGACTACGTGAAGAACATGATCACGGGCGCGGCGCAGATGGACGGCGCCATCCTGGTGGTGTC
GGCGGCGGACGGCCCGATGCCGCAGACGCGTGAGCACATCCTGCTGGCGCGCCAGGTCGGCGTTCCGTACATCGTGGTCT
TCCTGAACAAGGTGGACATGCTGGACGACCCCGAGCTGCGCGAGCTCGTGGAGATGGAAGTCCGCGACCTGCTGAAGAAG
TACGAGTTCCCTGGCGATGACATCCCCATCATCCCGGGCTCGGCGCTCAAGGCGCTGGAGGGTGACACCAGCGACATCGG
CGAGCCGGCCATCCTGAAGCTGATGGAGGCGGTGGACAGCTACATCCCGACGCCGCAGCGCGCGACGGACAAGCCCTTCC
TGATGCCGGTGGAGGACGTGTTCTCCATCTCCGGCCGCGGCACGGTGGCCACGGGCCGCGTCGAGCGCGGTATCATCAAG
GTCGGCGAGGAAGTGGAAGTCGTTGGTCTGCGCCCGACGCAGAAGACGGTCGTCACGGGCGTGGAGATGTTCCGCAAGCT
GCTGGACCAGGGCATGGCGGGCGACAACATCGGCGCGCTGGTGCGCGGCCTGAAGCGCGAGGACATGGAGCGCGGCCAGG
TGCTGGCCAAGCCGGGCAGCATCACCCCGCACACCAAGTTCAAGGCGCAGATCTACGTGCTGTCGAAGGAAGAGGGCGGT
CGTCACACCCCGTTCTTCAAGGGCTACCGCCCGCAGTTCTACTTCCGCACCACGGACGTGACGGGCTCGGTGAAGCTGCC
GGAGAACGTCGAAATGGTGATGCCGGGCGACAACATCGCCATTGAGGTGGAGCTCATCACCCCCGTGGCCATGGAGAAGG
AGCTGCGCTTCGCTGTCCGCGAGGGTGGCCGCACGGTGGGTGCCGGCGTCGTGGCGGAAATCGTCGAGTAG

Upstream 100 bases:

>100_bases
CGTTGTGGCAGGTTTGGAGCAGGACGCAGCACCTTCGCAGTTGTTTTCCGCGAGTCGAGACGTGCCGGGCTTTGGACCGG
CAACCCCGTGAGGAGAGGCC

Downstream 100 bases:

>100_bases
CGCAAACCCCCAGTGGCTCCCTGCGTTCCCAATTTCGGGAAATTTCAGGGAGCCGTTGGGAATCATGTTGCACACCCTGG
GGCGGGATGGTACACACCGC

Product: elongation factor Tu

Products: GDP; phosphate

Alternate protein names: EF-Tu 1 [H]

Number of amino acids: Translated: 396; Mature: 395

Protein sequence:

>396_residues
MAKEKFERNKPHVNIGTIGHVDHGKTSLTAAITKVLAKTGGATFLAYDLIDKAPEERERGITISTSHVEYQTSNRHYAHV
DCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLARQVGVPYIVVFLNKVDMLDDPELRELVEMEVRDLLKK
YEFPGDDIPIIPGSALKALEGDTSDIGEPAILKLMEAVDSYIPTPQRATDKPFLMPVEDVFSISGRGTVATGRVERGIIK
VGEEVEVVGLRPTQKTVVTGVEMFRKLLDQGMAGDNIGALVRGLKREDMERGQVLAKPGSITPHTKFKAQIYVLSKEEGG
RHTPFFKGYRPQFYFRTTDVTGSVKLPENVEMVMPGDNIAIEVELITPVAMEKELRFAVREGGRTVGAGVVAEIVE

Sequences:

>Translated_396_residues
MAKEKFERNKPHVNIGTIGHVDHGKTSLTAAITKVLAKTGGATFLAYDLIDKAPEERERGITISTSHVEYQTSNRHYAHV
DCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLARQVGVPYIVVFLNKVDMLDDPELRELVEMEVRDLLKK
YEFPGDDIPIIPGSALKALEGDTSDIGEPAILKLMEAVDSYIPTPQRATDKPFLMPVEDVFSISGRGTVATGRVERGIIK
VGEEVEVVGLRPTQKTVVTGVEMFRKLLDQGMAGDNIGALVRGLKREDMERGQVLAKPGSITPHTKFKAQIYVLSKEEGG
RHTPFFKGYRPQFYFRTTDVTGSVKLPENVEMVMPGDNIAIEVELITPVAMEKELRFAVREGGRTVGAGVVAEIVE
>Mature_395_residues
AKEKFERNKPHVNIGTIGHVDHGKTSLTAAITKVLAKTGGATFLAYDLIDKAPEERERGITISTSHVEYQTSNRHYAHVD
CPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLARQVGVPYIVVFLNKVDMLDDPELRELVEMEVRDLLKKY
EFPGDDIPIIPGSALKALEGDTSDIGEPAILKLMEAVDSYIPTPQRATDKPFLMPVEDVFSISGRGTVATGRVERGIIKV
GEEVEVVGLRPTQKTVVTGVEMFRKLLDQGMAGDNIGALVRGLKREDMERGQVLAKPGSITPHTKFKAQIYVLSKEEGGR
HTPFFKGYRPQFYFRTTDVTGSVKLPENVEMVMPGDNIAIEVELITPVAMEKELRFAVREGGRTVGAGVVAEIVE

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis [H]

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily [H]

Homologues:

Organism=Homo sapiens, GI34147630, Length=390, Percent_Identity=58.7179487179487, Blast_Score=488, Evalue=1e-138,
Organism=Homo sapiens, GI223555963, Length=441, Percent_Identity=31.5192743764172, Blast_Score=192, Evalue=4e-49,
Organism=Homo sapiens, GI5729864, Length=441, Percent_Identity=31.5192743764172, Blast_Score=191, Evalue=7e-49,
Organism=Homo sapiens, GI4503475, Length=450, Percent_Identity=30.8888888888889, Blast_Score=185, Evalue=5e-47,
Organism=Homo sapiens, GI4503471, Length=446, Percent_Identity=30.9417040358744, Blast_Score=182, Evalue=4e-46,
Organism=Homo sapiens, GI54607086, Length=345, Percent_Identity=31.0144927536232, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI194097354, Length=338, Percent_Identity=30.1775147928994, Blast_Score=129, Evalue=3e-30,
Organism=Homo sapiens, GI194018520, Length=338, Percent_Identity=30.1775147928994, Blast_Score=129, Evalue=3e-30,
Organism=Homo sapiens, GI194018522, Length=338, Percent_Identity=30.1775147928994, Blast_Score=129, Evalue=4e-30,
Organism=Homo sapiens, GI46094014, Length=338, Percent_Identity=29.585798816568, Blast_Score=125, Evalue=8e-29,
Organism=Homo sapiens, GI4503507, Length=269, Percent_Identity=29.7397769516729, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI157426893, Length=286, Percent_Identity=25.8741258741259, Blast_Score=77, Evalue=4e-14,
Organism=Homo sapiens, GI94966754, Length=148, Percent_Identity=34.4594594594595, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI53729339, Length=236, Percent_Identity=31.3559322033898, Blast_Score=67, Evalue=4e-11,
Organism=Homo sapiens, GI53729337, Length=236, Percent_Identity=31.3559322033898, Blast_Score=67, Evalue=4e-11,
Organism=Escherichia coli, GI1790412, Length=395, Percent_Identity=76.4556962025316, Blast_Score=607, Evalue=1e-175,
Organism=Escherichia coli, GI1789737, Length=395, Percent_Identity=76.4556962025316, Blast_Score=606, Evalue=1e-175,
Organism=Escherichia coli, GI2367247, Length=379, Percent_Identity=29.5514511873351, Blast_Score=137, Evalue=9e-34,
Organism=Escherichia coli, GI48994988, Length=242, Percent_Identity=34.7107438016529, Blast_Score=108, Evalue=5e-25,
Organism=Escherichia coli, GI1789108, Length=332, Percent_Identity=29.2168674698795, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI1788922, Length=138, Percent_Identity=33.3333333333333, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17556456, Length=400, Percent_Identity=52, Blast_Score=411, Evalue=1e-115,
Organism=Caenorhabditis elegans, GI25141371, Length=387, Percent_Identity=42.8940568475452, Blast_Score=330, Evalue=1e-90,
Organism=Caenorhabditis elegans, GI17552884, Length=449, Percent_Identity=31.6258351893096, Blast_Score=183, Evalue=2e-46,
Organism=Caenorhabditis elegans, GI17569207, Length=449, Percent_Identity=31.6258351893096, Blast_Score=183, Evalue=2e-46,
Organism=Caenorhabditis elegans, GI32566303, Length=420, Percent_Identity=30.7142857142857, Blast_Score=155, Evalue=4e-38,
Organism=Caenorhabditis elegans, GI115532067, Length=441, Percent_Identity=26.7573696145125, Blast_Score=139, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI115532065, Length=441, Percent_Identity=26.7573696145125, Blast_Score=138, Evalue=4e-33,
Organism=Caenorhabditis elegans, GI32566629, Length=454, Percent_Identity=27.0925110132159, Blast_Score=128, Evalue=5e-30,
Organism=Caenorhabditis elegans, GI17506081, Length=273, Percent_Identity=30.7692307692308, Blast_Score=116, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI32566301, Length=147, Percent_Identity=37.4149659863946, Blast_Score=95, Evalue=8e-20,
Organism=Caenorhabditis elegans, GI17509919, Length=281, Percent_Identity=27.7580071174377, Blast_Score=94, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17557151, Length=233, Percent_Identity=29.1845493562232, Blast_Score=86, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6324761, Length=396, Percent_Identity=67.4242424242424, Blast_Score=554, Evalue=1e-158,
Organism=Saccharomyces cerevisiae, GI6325337, Length=446, Percent_Identity=31.1659192825112, Blast_Score=181, Evalue=3e-46,
Organism=Saccharomyces cerevisiae, GI6319594, Length=446, Percent_Identity=31.1659192825112, Blast_Score=181, Evalue=3e-46,
Organism=Saccharomyces cerevisiae, GI6322937, Length=453, Percent_Identity=24.5033112582781, Blast_Score=112, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6320377, Length=346, Percent_Identity=29.1907514450867, Blast_Score=100, Evalue=4e-22,
Organism=Saccharomyces cerevisiae, GI6320863, Length=267, Percent_Identity=26.5917602996255, Blast_Score=82, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6323320, Length=241, Percent_Identity=29.4605809128631, Blast_Score=75, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6324550, Length=242, Percent_Identity=29.7520661157025, Blast_Score=69, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6323098, Length=176, Percent_Identity=31.25, Blast_Score=65, Evalue=1e-11,
Organism=Drosophila melanogaster, GI281363316, Length=389, Percent_Identity=58.6118251928021, Blast_Score=477, Evalue=1e-135,
Organism=Drosophila melanogaster, GI17864358, Length=389, Percent_Identity=58.6118251928021, Blast_Score=477, Evalue=1e-135,
Organism=Drosophila melanogaster, GI19921738, Length=386, Percent_Identity=48.7046632124352, Blast_Score=400, Evalue=1e-111,
Organism=Drosophila melanogaster, GI45553807, Length=457, Percent_Identity=31.7286652078775, Blast_Score=179, Evalue=4e-45,
Organism=Drosophila melanogaster, GI45553816, Length=457, Percent_Identity=31.7286652078775, Blast_Score=179, Evalue=4e-45,
Organism=Drosophila melanogaster, GI24651721, Length=457, Percent_Identity=31.7286652078775, Blast_Score=179, Evalue=4e-45,
Organism=Drosophila melanogaster, GI17864154, Length=457, Percent_Identity=31.7286652078775, Blast_Score=179, Evalue=4e-45,
Organism=Drosophila melanogaster, GI24652838, Length=456, Percent_Identity=30.7017543859649, Blast_Score=174, Evalue=8e-44,
Organism=Drosophila melanogaster, GI17137572, Length=456, Percent_Identity=30.7017543859649, Blast_Score=174, Evalue=8e-44,
Organism=Drosophila melanogaster, GI45550900, Length=447, Percent_Identity=29.082774049217, Blast_Score=144, Evalue=1e-34,
Organism=Drosophila melanogaster, GI17137380, Length=352, Percent_Identity=31.25, Blast_Score=133, Evalue=2e-31,
Organism=Drosophila melanogaster, GI19922690, Length=305, Percent_Identity=31.8032786885246, Blast_Score=132, Evalue=5e-31,
Organism=Drosophila melanogaster, GI24647046, Length=451, Percent_Identity=25.9423503325942, Blast_Score=96, Evalue=6e-20,
Organism=Drosophila melanogaster, GI24647048, Length=451, Percent_Identity=25.9423503325942, Blast_Score=95, Evalue=6e-20,
Organism=Drosophila melanogaster, GI28572034, Length=168, Percent_Identity=35.7142857142857, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI78706572, Length=246, Percent_Identity=27.6422764227642, Blast_Score=69, Evalue=5e-12,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3 [H]

EC number: 3.6.5.3

Molecular weight: Translated: 43417; Mature: 43285

Theoretical pI: Translated: 5.85; Mature: 5.85

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKEKFERNKPHVNIGTIGHVDHGKTSLTAAITKVLAKTGGATFLAYDLIDKAPEERERG
CCCCHHHCCCCEEEECEEECCCCCCHHHHHHHHHHHHHCCCCEEEEHHHHHCCCHHHHCC
ITISTSHVEYQTSNRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHI
EEEEECEEEEECCCCEEEEEECCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHH
LLARQVGVPYIVVFLNKVDMLDDPELRELVEMEVRDLLKKYEFPGDDIPIIPGSALKALE
HHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHC
GDTSDIGEPAILKLMEAVDSYIPTPQRATDKPFLMPVEDVFSISGRGTVATGRVERGIIK
CCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCEEEHHHHHCCCCCCEEEECCHHHHHHC
VGEEVEVVGLRPTQKTVVTGVEMFRKLLDQGMAGDNIGALVRGLKREDMERGQVLAKPGS
CCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCEEECCCC
ITPHTKFKAQIYVLSKEEGGRHTPFFKGYRPQFYFRTTDVTGSVKLPENVEMVMPGDNIA
CCCCCEEEEEEEEEEECCCCCCCCCCCCCCCEEEEEEECCCEEEECCCCCEEEECCCCEE
IEVELITPVAMEKELRFAVREGGRTVGAGVVAEIVE
EEEEEECCHHHHHHHHHHHHHCCCEECHHHHHHHCC
>Mature Secondary Structure 
AKEKFERNKPHVNIGTIGHVDHGKTSLTAAITKVLAKTGGATFLAYDLIDKAPEERERG
CCCHHHCCCCEEEECEEECCCCCCHHHHHHHHHHHHHCCCCEEEEHHHHHCCCHHHHCC
ITISTSHVEYQTSNRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHI
EEEEECEEEEECCCCEEEEEECCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHH
LLARQVGVPYIVVFLNKVDMLDDPELRELVEMEVRDLLKKYEFPGDDIPIIPGSALKALE
HHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHC
GDTSDIGEPAILKLMEAVDSYIPTPQRATDKPFLMPVEDVFSISGRGTVATGRVERGIIK
CCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCEEEHHHHHCCCCCCEEEECCHHHHHHC
VGEEVEVVGLRPTQKTVVTGVEMFRKLLDQGMAGDNIGALVRGLKREDMERGQVLAKPGS
CCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCEEECCCC
ITPHTKFKAQIYVLSKEEGGRHTPFFKGYRPQFYFRTTDVTGSVKLPENVEMVMPGDNIA
CCCCCEEEEEEEEEEECCCCCCCCCCCCCCCEEEEEEECCCEEEECCCCCEEEECCCCEE
IEVELITPVAMEKELRFAVREGGRTVGAGVVAEIVE
EEEEEECCHHHHHHHHHHHHHCCCEECHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA