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The map label for this gene is rlmI [H]

Identifier: 108758395

GI number: 108758395

Start: 3833668

End: 3834879

Strand: Direct

Name: rlmI [H]

Synonym: MXAN_3282

Alternate gene names: 108758395

Gene position: 3833668-3834879 (Clockwise)

Preceding gene: 108763808

Following gene: 108758039

Centisome position: 41.94

GC content: 68.73

Gene sequence:

>1212_bases
GTGAGGAGTGACACCGTGAATGTCGTGAAGCTGGAACTGGCGCGCGGACTGGGGCGCCACCTGCGCGCGGGGCACCCGTG
GGTGTTCCGCAAGGCGCTGGAGCACGTGCCGAAGATTCCGGCCGGCAGCGTGGTGGACCTGACGGAGAACGGGAAGTTCG
TGGCGCGCGGGTATTACGACCCGCACTCGGCCATCGCCGTGCGTGTGCTCACGCGCGACTCGCGTGACACCGTGGATGCG
GGCTTCATCACCCGGCGGGTGCGTCAGGCCCTGTCCGAGCGCACGGCGCTCATCGACCTCACCGACACGGACAGCTACCG
CCTGATTCATGGCGAGGGCGACGGCCTGCCGGGCGTGGTGGTGGACCTGTACGCGGGCTGGGCGGTGATGAAGCTGTACT
CCGCGGGCCTCACACCGTACCGGCCGCTCCTCATCGAGGCGCTGAAGGCCGGCGTGCCCGGGCTCAAGGGCATCATCGGC
CGTGACGAGGTCGGCCGCGACGACGTCGACGAGGACGAGGGACGCGGCTCCGGGAAGATGCTCTTCGGCCACGAGGCCCC
GGAGCTGATTCCCATCCGCGAGCGCGGGGCCACCTTCCTGGTGGACGCCTGGAAGGGGCAAAAGACGGGGTTCTTCCTGG
ATCAGCGGGAGAACCGCTACCTCATCCGTCGGCTGGGCAAGGGCCGCGACGTGCTCAATTGCTTCAGCTTCAGCGGCGGC
TTCTCCGTCAACGCGGCGCTGGGTGGGGCCAACAGCGTCTTCTCCGTGGATCAGGACCCGGACGCCATCGCGCTGGCTCG
GGAGAACTTCACGCGCAACGGCATCGCCGCGGAGAAGCACGACTTCCTGGCGGCGGACGTCTTCAAGATCATCCAGTCCT
TCAAGGACGAGGGCCGCACCTTCGACCTCATCATCCTGGACCCGCCCGCCTTCGCGAAGAGCCAGCGCGCGGTGCAGGCG
GCCATTGACGGGTATGCGTCCCTCAACCGGCAGGCCCTGGCCATCCTCCGGCCGGGTGGGTTGCTGGCGACGGCGTCGTG
CTCGGCGCGCGTGAGCCCGGACGACTTCATGGGCGCGGTGCGCGAGGCGGGGTTCAAGGCGGGCGTTGACCTGGCGCTCG
TCGAGGAGCGCTACCAGCCGCCGGACCACCCGGTGCGCTTGCAGTTCCCGGAGGGGAAGTACCTCAAGTTCTACGTGCTG
CAGTCCGTGTAG

Upstream 100 bases:

>100_bases
ATCGAGGCGCCACCTGCTTTCATCACCCGGGCGCCCGAGGGGCGGGGCTTCGAGGAACTGGCTCGCGCCATCCTGGCCCG
GAGGGGCCGCGGCGCGGCCC

Downstream 100 bases:

>100_bases
CCCGCCGCCGCGAATCGCGGGTTGAAACGCAAGAGGCCACGAGGTTCATCACCTCGTGGCCTCCGCTGTTTCCCGGTAGG
GAAGGGCGGCTCAGAAGTTG

Product: hypothetical protein

Products: NA

Alternate protein names: 23S rRNA m5C1962 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase RlmI [H]

Number of amino acids: Translated: 403; Mature: 403

Protein sequence:

>403_residues
MRSDTVNVVKLELARGLGRHLRAGHPWVFRKALEHVPKIPAGSVVDLTENGKFVARGYYDPHSAIAVRVLTRDSRDTVDA
GFITRRVRQALSERTALIDLTDTDSYRLIHGEGDGLPGVVVDLYAGWAVMKLYSAGLTPYRPLLIEALKAGVPGLKGIIG
RDEVGRDDVDEDEGRGSGKMLFGHEAPELIPIRERGATFLVDAWKGQKTGFFLDQRENRYLIRRLGKGRDVLNCFSFSGG
FSVNAALGGANSVFSVDQDPDAIALARENFTRNGIAAEKHDFLAADVFKIIQSFKDEGRTFDLIILDPPAFAKSQRAVQA
AIDGYASLNRQALAILRPGGLLATASCSARVSPDDFMGAVREAGFKAGVDLALVEERYQPPDHPVRLQFPEGKYLKFYVL
QSV

Sequences:

>Translated_403_residues
MRSDTVNVVKLELARGLGRHLRAGHPWVFRKALEHVPKIPAGSVVDLTENGKFVARGYYDPHSAIAVRVLTRDSRDTVDA
GFITRRVRQALSERTALIDLTDTDSYRLIHGEGDGLPGVVVDLYAGWAVMKLYSAGLTPYRPLLIEALKAGVPGLKGIIG
RDEVGRDDVDEDEGRGSGKMLFGHEAPELIPIRERGATFLVDAWKGQKTGFFLDQRENRYLIRRLGKGRDVLNCFSFSGG
FSVNAALGGANSVFSVDQDPDAIALARENFTRNGIAAEKHDFLAADVFKIIQSFKDEGRTFDLIILDPPAFAKSQRAVQA
AIDGYASLNRQALAILRPGGLLATASCSARVSPDDFMGAVREAGFKAGVDLALVEERYQPPDHPVRLQFPEGKYLKFYVL
QSV
>Mature_403_residues
MRSDTVNVVKLELARGLGRHLRAGHPWVFRKALEHVPKIPAGSVVDLTENGKFVARGYYDPHSAIAVRVLTRDSRDTVDA
GFITRRVRQALSERTALIDLTDTDSYRLIHGEGDGLPGVVVDLYAGWAVMKLYSAGLTPYRPLLIEALKAGVPGLKGIIG
RDEVGRDDVDEDEGRGSGKMLFGHEAPELIPIRERGATFLVDAWKGQKTGFFLDQRENRYLIRRLGKGRDVLNCFSFSGG
FSVNAALGGANSVFSVDQDPDAIALARENFTRNGIAAEKHDFLAADVFKIIQSFKDEGRTFDLIILDPPAFAKSQRAVQA
AIDGYASLNRQALAILRPGGLLATASCSARVSPDDFMGAVREAGFKAGVDLALVEERYQPPDHPVRLQFPEGKYLKFYVL
QSV

Specific function: Specifically methylates the cytosine at position 1962 (m5C1962) of 23S rRNA [H]

COG id: COG1092

COG function: function code R; Predicted SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Escherichia coli, GI145693111, Length=393, Percent_Identity=39.1857506361323, Blast_Score=285, Evalue=3e-78,
Organism=Escherichia coli, GI1787181, Length=281, Percent_Identity=29.1814946619217, Blast_Score=101, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002478
- InterPro:   IPR015947
- InterPro:   IPR019614 [H]

Pfam domain/function: PF10672 Methyltrans_SAM [H]

EC number: =2.1.1.191 [H]

Molecular weight: Translated: 44168; Mature: 44168

Theoretical pI: Translated: 8.21; Mature: 8.21

Prosite motif: PS50890 PUA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSDTVNVVKLELARGLGRHLRAGHPWVFRKALEHVPKIPAGSVVDLTENGKFVARGYYD
CCCCCEEHEEEHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCEEEEECCCCEEEEECCC
PHSAIAVRVLTRDSRDTVDAGFITRRVRQALSERTALIDLTDTDSYRLIHGEGDGLPGVV
CCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHCEEEEEEECCCCEEEEECCCCCCCHHH
VDLYAGWAVMKLYSAGLTPYRPLLIEALKAGVPGLKGIIGRDEVGRDDVDEDEGRGSGKM
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCE
LFGHEAPELIPIRERGATFLVDAWKGQKTGFFLDQRENRYLIRRLGKGRDVLNCFSFSGG
EECCCCCCEEEECCCCCEEEEECCCCCCCCEEEECCCCHHHHHHHCCCCCEEEEEECCCC
FSVNAALGGANSVFSVDQDPDAIALARENFTRNGIAAEKHDFLAADVFKIIQSFKDEGRT
CEEEEECCCCCCEEECCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCE
FDLIILDPPAFAKSQRAVQAAIDGYASLNRQALAILRPGGLLATASCSARVSPDDFMGAV
EEEEEECCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEECCCCCCCCHHHHHHHH
REAGFKAGVDLALVEERYQPPDHPVRLQFPEGKYLKFYVLQSV
HHCCHHCCCCEEEHHHHCCCCCCCEEEECCCCCEEEEEEEECC
>Mature Secondary Structure
MRSDTVNVVKLELARGLGRHLRAGHPWVFRKALEHVPKIPAGSVVDLTENGKFVARGYYD
CCCCCEEHEEEHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCEEEEECCCCEEEEECCC
PHSAIAVRVLTRDSRDTVDAGFITRRVRQALSERTALIDLTDTDSYRLIHGEGDGLPGVV
CCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHCEEEEEEECCCCEEEEECCCCCCCHHH
VDLYAGWAVMKLYSAGLTPYRPLLIEALKAGVPGLKGIIGRDEVGRDDVDEDEGRGSGKM
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCE
LFGHEAPELIPIRERGATFLVDAWKGQKTGFFLDQRENRYLIRRLGKGRDVLNCFSFSGG
EECCCCCCEEEECCCCCEEEEECCCCCCCCEEEECCCCHHHHHHHCCCCCEEEEEECCCC
FSVNAALGGANSVFSVDQDPDAIALARENFTRNGIAAEKHDFLAADVFKIIQSFKDEGRT
CEEEEECCCCCCEEECCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCE
FDLIILDPPAFAKSQRAVQAAIDGYASLNRQALAILRPGGLLATASCSARVSPDDFMGAV
EEEEEECCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEECCCCCCCCHHHHHHHH
REAGFKAGVDLALVEERYQPPDHPVRLQFPEGKYLKFYVLQSV
HHCCHHCCCCEEEHHHHCCCCCCCEEEECCCCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA