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The map label for this gene is rpoN [H]

Identifier: 108758300

GI number: 108758300

Start: 1238621

End: 1240138

Strand: Direct

Name: rpoN [H]

Synonym: MXAN_1061

Alternate gene names: 108758300

Gene position: 1238621-1240138 (Clockwise)

Preceding gene: 108757943

Following gene: 108760357

Centisome position: 13.55

GC content: 64.36

Gene sequence:

>1518_bases
ATGGCGATGGAACTGAAACAAAGCCTGAAGCTTGCCCAACAGCTGGTGATGACGCCGCAGCTGCAGCAAGCCATCAAGCT
CCTCCAACTGTCTCGCATGGAACTGCTCGAGCAGGTCCGCGAGGAGATGGAGCAGAACCCGCTGCTGGAGCAACCAGACG
AGCAAGCGCCGGGGGATGTGGGAGACAAGGAACCCGGGGAAGCCTCCCTGGAAGCAGACAACATGGAGGTGCCGCGGGAC
GTGGATCTGCCCGCGGCCACCAGCGACACCGCCACGGAGTTCAAGGCGGACGGGGAGGGCCCCCCGGAAATCGACTGGGA
GCAGTACCTCAACAGCTACCAGTTCAATGAGCCCACCACGGCCTCCAACAAGGGCAACGTGGCCACGGACGACATGCCGT
CGTTCGAGGCCAACCTCGTCAAGAAGGCGGACCTGGTCGACCACATCCAGGAGCAGCTGGGCACGCTGCGCCTGAATGAC
GCCGAGCGCCGCATCGCCATGCTCATCCTGGGCAACCTGGATGACGACGGCTACCTCAAGCTGCCGGAAGTGGACGGGGA
TCCGCTCATCCGCCTGGCCAACGAGGCGGACGTGCCCATGCACGTCGCGGAGCGCACGCTGCGGCGCATCCAGATGTTGG
ATCCGCGCGGCTGCGGCGCCCGTGACTTGCAGGAGTGCCTGCTCATCCAGCTCCAGGGCATCAGGGAGCCGCACGCGCCG
CTGTTGGGCCTCATCATCAAGCGGCACATGAAGTACCTGGAGAGCAAGAACCTGCCCGCCATCGCCAAGGACCTGAAGGT
CACCTTGGAAGAGGTGGTGGGGGCGGTGCGGCTGCTCCCGAAGCTGGACCCGAAGCCGGGCCGCAACTTCAGCGGGGACG
ACGCGCAGTACATCTCCCCCGACGTGTTCGTCTACAAGATGGGGGACGACTACACGGTGGTGCTCAACGATGACGGCCTG
TCCAAGCTGCGCATCTCCGGCACCTACCGGAACGCGCTGAAGACGGGCGCGGTGGGCCCGGGCCAGACGAAGGACTTCAT
CCAGGACAAGCTGCGCAGCGCGATGTGGCTCATCCGCTCCATCCACCAGCGGCAGCGGACCATCTACAAAGTCACCGAGA
GCATCGTGAAGTTCCAGCGGGACTTCCTGGACAAGGGCATTGCCTACCTCAAGCCGCTCATCCTCCGGGACGTGGCCGAG
GACATCGGCATGCACGAGTCCACGGTGAGCCGCGTCACCACCAGCAAGTACGTGCACACACCGCAGGGCATCTTCGAGCT
GAAGTACTTCTTCAACTCGTCCATCGCCCGCGTCTCCGGTGAGGACACCGCGAGCGAGGCGGTGAAGCACCACATCAAGC
AGTTGGTGGCGCAGGAAGACGCCCGCAACCCGTACTCGGACCAGAAAATCGTCGAGCTGCTGCGCTCGCAGGGCACCGAG
ATTGCCCGCCGCACGGTGGCCAAGTACCGCGAGGTGCTGGGCATCCTCCCCAGCAGCAAGCGCAAGCGGTACTACTGA

Upstream 100 bases:

>100_bases
TCGGGGCTAGACGAAGTCAGGGCTCCTTGTTACGTTGGCACGGTCTTTGATGGTGGTCCTCTGTCCAGAGGGACCGAATC
AACCCGGGAGACCCCGTCTC

Downstream 100 bases:

>100_bases
GTCAGGGCTGTTCGGGCGCGCCGCTTGTCGTCGGCGCGCCCGGGGGGGCAGAGTGGGCGTCCCATGAGCGCCACCCTCCC
TCCCGATTCCGCCGTGCACC

Product: RNA polymerase sigma-54 factor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 505; Mature: 504

Protein sequence:

>505_residues
MAMELKQSLKLAQQLVMTPQLQQAIKLLQLSRMELLEQVREEMEQNPLLEQPDEQAPGDVGDKEPGEASLEADNMEVPRD
VDLPAATSDTATEFKADGEGPPEIDWEQYLNSYQFNEPTTASNKGNVATDDMPSFEANLVKKADLVDHIQEQLGTLRLND
AERRIAMLILGNLDDDGYLKLPEVDGDPLIRLANEADVPMHVAERTLRRIQMLDPRGCGARDLQECLLIQLQGIREPHAP
LLGLIIKRHMKYLESKNLPAIAKDLKVTLEEVVGAVRLLPKLDPKPGRNFSGDDAQYISPDVFVYKMGDDYTVVLNDDGL
SKLRISGTYRNALKTGAVGPGQTKDFIQDKLRSAMWLIRSIHQRQRTIYKVTESIVKFQRDFLDKGIAYLKPLILRDVAE
DIGMHESTVSRVTTSKYVHTPQGIFELKYFFNSSIARVSGEDTASEAVKHHIKQLVAQEDARNPYSDQKIVELLRSQGTE
IARRTVAKYREVLGILPSSKRKRYY

Sequences:

>Translated_505_residues
MAMELKQSLKLAQQLVMTPQLQQAIKLLQLSRMELLEQVREEMEQNPLLEQPDEQAPGDVGDKEPGEASLEADNMEVPRD
VDLPAATSDTATEFKADGEGPPEIDWEQYLNSYQFNEPTTASNKGNVATDDMPSFEANLVKKADLVDHIQEQLGTLRLND
AERRIAMLILGNLDDDGYLKLPEVDGDPLIRLANEADVPMHVAERTLRRIQMLDPRGCGARDLQECLLIQLQGIREPHAP
LLGLIIKRHMKYLESKNLPAIAKDLKVTLEEVVGAVRLLPKLDPKPGRNFSGDDAQYISPDVFVYKMGDDYTVVLNDDGL
SKLRISGTYRNALKTGAVGPGQTKDFIQDKLRSAMWLIRSIHQRQRTIYKVTESIVKFQRDFLDKGIAYLKPLILRDVAE
DIGMHESTVSRVTTSKYVHTPQGIFELKYFFNSSIARVSGEDTASEAVKHHIKQLVAQEDARNPYSDQKIVELLRSQGTE
IARRTVAKYREVLGILPSSKRKRYY
>Mature_504_residues
AMELKQSLKLAQQLVMTPQLQQAIKLLQLSRMELLEQVREEMEQNPLLEQPDEQAPGDVGDKEPGEASLEADNMEVPRDV
DLPAATSDTATEFKADGEGPPEIDWEQYLNSYQFNEPTTASNKGNVATDDMPSFEANLVKKADLVDHIQEQLGTLRLNDA
ERRIAMLILGNLDDDGYLKLPEVDGDPLIRLANEADVPMHVAERTLRRIQMLDPRGCGARDLQECLLIQLQGIREPHAPL
LGLIIKRHMKYLESKNLPAIAKDLKVTLEEVVGAVRLLPKLDPKPGRNFSGDDAQYISPDVFVYKMGDDYTVVLNDDGLS
KLRISGTYRNALKTGAVGPGQTKDFIQDKLRSAMWLIRSIHQRQRTIYKVTESIVKFQRDFLDKGIAYLKPLILRDVAED
IGMHESTVSRVTTSKYVHTPQGIFELKYFFNSSIARVSGEDTASEAVKHHIKQLVAQEDARNPYSDQKIVELLRSQGTEI
ARRTVAKYREVLGILPSSKRKRYY

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released [H]

COG id: COG1508

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma54 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-54 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789594, Length=498, Percent_Identity=36.3453815261044, Blast_Score=309, Evalue=2e-85,

Paralogues:

None

Copy number: 70 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000394
- InterPro:   IPR007046
- InterPro:   IPR007634 [H]

Pfam domain/function: PF00309 Sigma54_AID; PF04963 Sigma54_CBD; PF04552 Sigma54_DBD [H]

EC number: NA

Molecular weight: Translated: 56975; Mature: 56844

Theoretical pI: Translated: 5.33; Mature: 5.33

Prosite motif: PS00717 SIGMA54_1 ; PS00718 SIGMA54_2 ; PS50044 SIGMA54_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAMELKQSLKLAQQLVMTPQLQQAIKLLQLSRMELLEQVREEMEQNPLLEQPDEQAPGDV
CCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
GDKEPGEASLEADNMEVPRDVDLPAATSDTATEFKADGEGPPEIDWEQYLNSYQFNEPTT
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCHHHHHCCCCCCCCCC
ASNKGNVATDDMPSFEANLVKKADLVDHIQEQLGTLRLNDAERRIAMLILGNLDDDGYLK
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCEEECCHHHHEEEEEEECCCCCCCEE
LPEVDGDPLIRLANEADVPMHVAERTLRRIQMLDPRGCGARDLQECLLIQLQGIREPHAP
CCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCHH
LLGLIIKRHMKYLESKNLPAIAKDLKVTLEEVVGAVRLLPKLDPKPGRNFSGDDAQYISP
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
DVFVYKMGDDYTVVLNDDGLSKLRISGTYRNALKTGAVGPGQTKDFIQDKLRSAMWLIRS
CEEEEEECCCEEEEECCCCCCEEEECCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHH
IHQRQRTIYKVTESIVKFQRDFLDKGIAYLKPLILRDVAEDIGMHESTVSRVTTSKYVHT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCC
PQGIFELKYFFNSSIARVSGEDTASEAVKHHIKQLVAQEDARNPYSDQKIVELLRSQGTE
CCHHHHHHHHHCCCHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCHH
IARRTVAKYREVLGILPSSKRKRYY
HHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
AMELKQSLKLAQQLVMTPQLQQAIKLLQLSRMELLEQVREEMEQNPLLEQPDEQAPGDV
CCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
GDKEPGEASLEADNMEVPRDVDLPAATSDTATEFKADGEGPPEIDWEQYLNSYQFNEPTT
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCHHHHHCCCCCCCCCC
ASNKGNVATDDMPSFEANLVKKADLVDHIQEQLGTLRLNDAERRIAMLILGNLDDDGYLK
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCEEECCHHHHEEEEEEECCCCCCCEE
LPEVDGDPLIRLANEADVPMHVAERTLRRIQMLDPRGCGARDLQECLLIQLQGIREPHAP
CCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCHH
LLGLIIKRHMKYLESKNLPAIAKDLKVTLEEVVGAVRLLPKLDPKPGRNFSGDDAQYISP
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
DVFVYKMGDDYTVVLNDDGLSKLRISGTYRNALKTGAVGPGQTKDFIQDKLRSAMWLIRS
CEEEEEECCCEEEEECCCCCCEEEECCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHH
IHQRQRTIYKVTESIVKFQRDFLDKGIAYLKPLILRDVAEDIGMHESTVSRVTTSKYVHT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCC
PQGIFELKYFFNSSIARVSGEDTASEAVKHHIKQLVAQEDARNPYSDQKIVELLRSQGTE
CCHHHHHHHHHCCCHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCHH
IARRTVAKYREVLGILPSSKRKRYY
HHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9421909 [H]