Click here to switch to the map view.

The map label for this gene is cheB

Identifier: 108758247

GI number: 108758247

Start: 5954512

End: 5955549

Strand: Reverse

Name: cheB

Synonym: MXAN_4752

Alternate gene names: 108758247

Gene position: 5955549-5954512 (Counterclockwise)

Preceding gene: 108761128

Following gene: 108759520

Centisome position: 65.16

GC content: 71.58

Gene sequence:

>1038_bases
ATGAGCAGCAAGGGCGTCATCTCCGTACTGGTCATCGACGACTCTGCCCACAACCGGCGGACCCTCTCCACGATGTTGGA
GTCGGAGCCGGACGTGCGAGTGGTGGACCGCGCGGCGGATGGCGAAGAGGGGCTGAAGAAGGTCGTGGACCTGAAGCCCG
ACGTGGTGACGCTGGACCTGGAGATGCCGAAGCTCGGCGGGTACACATTCCTCCGCCTGCTCATGCGCACGGCGCCCACG
CCCGTCATCGTCATCTCCAGCTATTCGCACCGCTCGGATGTCTTCAAGGCGCTGGAGTTGGGGGCATTCGACTTCATCGC
CAAGCCGCAGCACCCGACGTTCGAGGCCATGGAGGCGCTTCGGGTGGAGCTGCTGGAGAAGGTCCGGGCGGCGCGGCACG
TGCGCCCCGGTTACCGGCACGCGGCGCCAGGGGCTCGCGCCATGGCGGTGGCGGGCGAGCCGCCACTGGTGGTGGCCGTG
GGCGCCTCCACGGGGGGCCCCCCCGCGGTGCAGCGGGTGCTGGAGGGACTCGCCGTGGAGCCCACGCCGTGCGTGTTGGT
CTGCCAGCACATGCCGCCGCAGTTCACCCGCGCCTTCGCGGACCGGTTGGACCGCATCGGCCCCTTCTCGGTGACGGAGG
CTCGTGACGGAGACCTGGTACAGCCCGGGCACGTGTACATCGCTCCAGGCGGGCGGCACATGGTGGTGGCCGAGCGGGGC
TCCCGGCTGGAGCTGCAAACGCCGCCGCCCACGCCCGTGGACAAGTACGCGCCCTGTGTGGACCGCCTCTTCGTCAGCAT
GGCGCAGGTGCTCGGCGCCAAGGCCCTGGGGGTGGTGCTCACGGGCATGGGCGCCGACGGTGCGGAGGGCGCGCGCGCGG
TGCACCGCGCTGGCGGCGAGGTCTGGGCGCAGTCGGAGGAGACCTCGGTGGTGTTCGGCATGCCGGGGGAGGCCATCGCC
ACCGGGGCGGTGAAGCGGGTGATTCCACTGGGTGACATCGGCCCGGCGCTCGCGGCCCTGGCGCGGCGGCGGCGCTGA

Upstream 100 bases:

>100_bases
ACTCAGAGAACCTGCTGAACCTGGGCTCGGACTTCGAGTTCGTTCACCTGCGCGGGGACCTGGTGTACCGGAGGCCGGAG
CTGGACGAAGGAGCGCGTCG

Downstream 100 bases:

>100_bases
AACTCCGGCCCCTGGCGCACGGGTGCTAACGTCCCCGGCCCATGACGCAGCAGATTCGAGCCTTGGTGGTGGATGACTCG
CAGGCCATGCGGCGCAGCAT

Product: protein-glutamate methylesterase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 345; Mature: 344

Protein sequence:

>345_residues
MSSKGVISVLVIDDSAHNRRTLSTMLESEPDVRVVDRAADGEEGLKKVVDLKPDVVTLDLEMPKLGGYTFLRLLMRTAPT
PVIVISSYSHRSDVFKALELGAFDFIAKPQHPTFEAMEALRVELLEKVRAARHVRPGYRHAAPGARAMAVAGEPPLVVAV
GASTGGPPAVQRVLEGLAVEPTPCVLVCQHMPPQFTRAFADRLDRIGPFSVTEARDGDLVQPGHVYIAPGGRHMVVAERG
SRLELQTPPPTPVDKYAPCVDRLFVSMAQVLGAKALGVVLTGMGADGAEGARAVHRAGGEVWAQSEETSVVFGMPGEAIA
TGAVKRVIPLGDIGPALAALARRRR

Sequences:

>Translated_345_residues
MSSKGVISVLVIDDSAHNRRTLSTMLESEPDVRVVDRAADGEEGLKKVVDLKPDVVTLDLEMPKLGGYTFLRLLMRTAPT
PVIVISSYSHRSDVFKALELGAFDFIAKPQHPTFEAMEALRVELLEKVRAARHVRPGYRHAAPGARAMAVAGEPPLVVAV
GASTGGPPAVQRVLEGLAVEPTPCVLVCQHMPPQFTRAFADRLDRIGPFSVTEARDGDLVQPGHVYIAPGGRHMVVAERG
SRLELQTPPPTPVDKYAPCVDRLFVSMAQVLGAKALGVVLTGMGADGAEGARAVHRAGGEVWAQSEETSVVFGMPGEAIA
TGAVKRVIPLGDIGPALAALARRRR
>Mature_344_residues
SSKGVISVLVIDDSAHNRRTLSTMLESEPDVRVVDRAADGEEGLKKVVDLKPDVVTLDLEMPKLGGYTFLRLLMRTAPTP
VIVISSYSHRSDVFKALELGAFDFIAKPQHPTFEAMEALRVELLEKVRAARHVRPGYRHAAPGARAMAVAGEPPLVVAVG
ASTGGPPAVQRVLEGLAVEPTPCVLVCQHMPPQFTRAFADRLDRIGPFSVTEARDGDLVQPGHVYIAPGGRHMVVAERGS
RLELQTPPPTPVDKYAPCVDRLFVSMAQVLGAKALGVVLTGMGADGAEGARAVHRAGGEVWAQSEETSVVFGMPGEAIAT
GAVKRVIPLGDIGPALAALARRRR

Specific function: Involved in the modulation of the chemotaxis system; catalyzes the demethylation of specific methylglutamate residues introduced into the chemoreceptors (methyl-accepting chemotaxis proteins) by CheR

COG id: COG2201

COG function: function code NT; Chemotaxis response regulator containing a CheY-like receiver domain and a methylesterase domain

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain

Homologues:

Organism=Escherichia coli, GI1788192, Length=339, Percent_Identity=41.2979351032448, Blast_Score=243, Evalue=2e-65,
Organism=Escherichia coli, GI1786911, Length=102, Percent_Identity=39.2156862745098, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI87082052, Length=104, Percent_Identity=29.8076923076923, Blast_Score=61, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CHEB1_MYXXD (Q1D359)

Other databases:

- EMBL:   CP000113
- RefSeq:   YP_632914.1
- ProteinModelPortal:   Q1D359
- SMR:   Q1D359
- IntAct:   Q1D359
- STRING:   Q1D359
- GeneID:   4102107
- GenomeReviews:   CP000113_GR
- KEGG:   mxa:MXAN_4752
- TIGR:   MXAN_4752
- eggNOG:   COG2201
- HOGENOM:   HBG705324
- OMA:   SQHMPAG
- PhylomeDB:   Q1D359
- BioCyc:   MXAN246197:MXAN_4752-MONOMER
- GO:   GO:0005737
- GO:   GO:0006935
- HAMAP:   MF_00099
- InterPro:   IPR011006
- InterPro:   IPR008248
- InterPro:   IPR000673
- InterPro:   IPR001789
- Gene3D:   G3DSA:3.40.50.180
- PIRSF:   PIRSF000876
- SMART:   SM00448

Pfam domain/function: PF01339 CheB_methylest; PF00072 Response_reg; SSF52738 Chemotax_RR_pGlu_Me-esterase; SSF52172 CheY_like

EC number: =3.1.1.61

Molecular weight: Translated: 36756; Mature: 36625

Theoretical pI: Translated: 8.12; Mature: 8.12

Prosite motif: PS50122 CHEB; PS50110 RESPONSE_REGULATORY

Important sites: ACT_SITE 163-163 ACT_SITE 190-190 ACT_SITE 286-286

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSKGVISVLVIDDSAHNRRTLSTMLESEPDVRVVDRAADGEEGLKKVVDLKPDVVTLDL
CCCCCEEEEEEEECCCCCHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHCCCCCEEEEEE
EMPKLGGYTFLRLLMRTAPTPVIVISSYSHRSDVFKALELGAFDFIAKPQHPTFEAMEAL
CCCCCCHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHCCHHHHCCCCCCCHHHHHHH
RVELLEKVRAARHVRPGYRHAAPGARAMAVAGEPPLVVAVGASTGGPPAVQRVLEGLAVE
HHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCCEEEEECCCCCCCHHHHHHHHCCCCC
PTPCVLVCQHMPPQFTRAFADRLDRIGPFSVTEARDGDLVQPGHVYIAPGGRHMVVAERG
CCCCEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEECCCCCEEEEEECC
SRLELQTPPPTPVDKYAPCVDRLFVSMAQVLGAKALGVVLTGMGADGAEGARAVHRAGGE
CEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCHHHHHHEEECCCCCCCHHHHHHHHCCCC
VWAQSEETSVVFGMPGEAIATGAVKRVIPLGDIGPALAALARRRR
EEECCCCCEEEEECCCHHHHHHHHHHHCCCCCCCHHHHHHHHHCC
>Mature Secondary Structure 
SSKGVISVLVIDDSAHNRRTLSTMLESEPDVRVVDRAADGEEGLKKVVDLKPDVVTLDL
CCCCEEEEEEEECCCCCHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHCCCCCEEEEEE
EMPKLGGYTFLRLLMRTAPTPVIVISSYSHRSDVFKALELGAFDFIAKPQHPTFEAMEAL
CCCCCCHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHCCHHHHCCCCCCCHHHHHHH
RVELLEKVRAARHVRPGYRHAAPGARAMAVAGEPPLVVAVGASTGGPPAVQRVLEGLAVE
HHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCCEEEEECCCCCCCHHHHHHHHCCCCC
PTPCVLVCQHMPPQFTRAFADRLDRIGPFSVTEARDGDLVQPGHVYIAPGGRHMVVAERG
CCCCEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEECCCCCEEEEEECC
SRLELQTPPPTPVDKYAPCVDRLFVSMAQVLGAKALGVVLTGMGADGAEGARAVHRAGGE
CEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCHHHHHHEEECCCCCCCHHHHHHHHCCCC
VWAQSEETSVVFGMPGEAIATGAVKRVIPLGDIGPALAALARRRR
EEECCCCCEEEEECCCHHHHHHHHHHHCCCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA