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The map label for this gene is ampD [H]

Identifier: 108758072

GI number: 108758072

Start: 1140175

End: 1140786

Strand: Reverse

Name: ampD [H]

Synonym: MXAN_0987

Alternate gene names: 108758072

Gene position: 1140786-1140175 (Counterclockwise)

Preceding gene: 108762817

Following gene: 108757229

Centisome position: 12.48

GC content: 64.71

Gene sequence:

>612_bases
GTGAAGAAGTTCCAGGCGGACAACAAGCTGCCCACCACCGGTTTCTACGGTGACCTCACGCAGGCGGCGCTGAAGAAGGC
GCTGGGCACCAGCGGCCCCACCACGCCCACCACCCCGGGCGGCAAGTTCACCAAGCCCGCGGTCATCAACGCGCCCTCGC
CGAACCAGAACTCGCGCGGCGGGACGAAGATCGACACCATCGTCATGCACCACACCGCGACCAACAACGGCGCGGGCGAC
CTGTCGTGGATGCGCAATCCGCAGAGCAAGGTGTCCGCGCACTACATGGTGGACCGCGACGGGAAGATCTATCAGCTGGT
CGGTGACGACAAGCGCGCCTGGCACGCGGGCAAGGGCGAGCTGCACGGCGTGCCCTCGGACATCAACAACCGCTCCATCG
GCATCGAAATCGTGAACGACGGCAGCGGCAAGACGCCCTTCACCGAGGCGCAGTACAAGTCACTCACCCAGCTCACCGGT
TACCTGAAGCAGCAGCACAACATTCCGATGAAGAACATCGTGGGCCACGCCGACGTGGCGGTTCCCAAGGGCCGCAAGAA
CGACCCGGCGCCCAACTTCGATTGGAACCGGCTGCGCAAGGGCATCTCCTGA

Upstream 100 bases:

>100_bases
GGTGAAGCAACTGCAGGATGCGCTCGTGAAGGTGGGCTACATGACGAAGGCCCAGGTGACCACGGGCCCCGGCACCTTCG
GTCCGCAGACGGAAACGGCG

Downstream 100 bases:

>100_bases
CAGTCGTCTTCCCCTGCGTGCGAGGAGGCTCGCTGAGCGTGAGCCCCTCGCACGCATCGCGTTTGGAAACGTGGTAGAAG
GTCGCCAGCGTGCTCCGAAT

Product: N-acetylmuramoyl-L-alanine amidase

Products: NA

Alternate protein names: N-acetylmuramoyl-L-alanine amidase [H]

Number of amino acids: Translated: 203; Mature: 203

Protein sequence:

>203_residues
MKKFQADNKLPTTGFYGDLTQAALKKALGTSGPTTPTTPGGKFTKPAVINAPSPNQNSRGGTKIDTIVMHHTATNNGAGD
LSWMRNPQSKVSAHYMVDRDGKIYQLVGDDKRAWHAGKGELHGVPSDINNRSIGIEIVNDGSGKTPFTEAQYKSLTQLTG
YLKQQHNIPMKNIVGHADVAVPKGRKNDPAPNFDWNRLRKGIS

Sequences:

>Translated_203_residues
MKKFQADNKLPTTGFYGDLTQAALKKALGTSGPTTPTTPGGKFTKPAVINAPSPNQNSRGGTKIDTIVMHHTATNNGAGD
LSWMRNPQSKVSAHYMVDRDGKIYQLVGDDKRAWHAGKGELHGVPSDINNRSIGIEIVNDGSGKTPFTEAQYKSLTQLTG
YLKQQHNIPMKNIVGHADVAVPKGRKNDPAPNFDWNRLRKGIS
>Mature_203_residues
MKKFQADNKLPTTGFYGDLTQAALKKALGTSGPTTPTTPGGKFTKPAVINAPSPNQNSRGGTKIDTIVMHHTATNNGAGD
LSWMRNPQSKVSAHYMVDRDGKIYQLVGDDKRAWHAGKGELHGVPSDINNRSIGIEIVNDGSGKTPFTEAQYKSLTQLTG
YLKQQHNIPMKNIVGHADVAVPKGRKNDPAPNFDWNRLRKGIS

Specific function: Involved in both cell wall peptidoglycans recycling and beta-lactamase induction. Specifically cleaves the amide bond between the lactyl group of N-acetylmuramic acid and the alpha- amino group of the L-alanine in degradation products containing an anhydr

COG id: COG3023

COG function: function code V; Negative regulator of beta-lactamase expression

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family [H]

Homologues:

Organism=Escherichia coli, GI1786300, Length=172, Percent_Identity=35.4651162790698, Blast_Score=93, Evalue=1e-20,
Organism=Escherichia coli, GI1787092, Length=155, Percent_Identity=36.1290322580645, Blast_Score=78, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002502 [H]

Pfam domain/function: PF01510 Amidase_2 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 22016; Mature: 22016

Theoretical pI: Translated: 10.50; Mature: 10.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKFQADNKLPTTGFYGDLTQAALKKALGTSGPTTPTTPGGKFTKPAVINAPSPNQNSRG
CCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCC
GTKIDTIVMHHTATNNGAGDLSWMRNPQSKVSAHYMVDRDGKIYQLVGDDKRAWHAGKGE
CCEEEEEEEEECCCCCCCCCCHHHCCCHHHCEEEEEECCCCCEEEEECCCCCEEECCCCC
LHGVPSDINNRSIGIEIVNDGSGKTPFTEAQYKSLTQLTGYLKQQHNIPMKNIVGHADVA
CCCCCCCCCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCEE
VPKGRKNDPAPNFDWNRLRKGIS
CCCCCCCCCCCCCCHHHHHCCCC
>Mature Secondary Structure
MKKFQADNKLPTTGFYGDLTQAALKKALGTSGPTTPTTPGGKFTKPAVINAPSPNQNSRG
CCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCC
GTKIDTIVMHHTATNNGAGDLSWMRNPQSKVSAHYMVDRDGKIYQLVGDDKRAWHAGKGE
CCEEEEEEEEECCCCCCCCCCHHHCCCHHHCEEEEEECCCCCEEEEECCCCCEEECCCCC
LHGVPSDINNRSIGIEIVNDGSGKTPFTEAQYKSLTQLTGYLKQQHNIPMKNIVGHADVA
CCCCCCCCCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCEE
VPKGRKNDPAPNFDWNRLRKGIS
CCCCCCCCCCCCCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2691840; 2607970; 9278503; 7959070 [H]