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The map label for this gene is frdB [C]

Identifier: 108758026

GI number: 108758026

Start: 6520839

End: 6521651

Strand: Reverse

Name: frdB [C]

Synonym: MXAN_5222

Alternate gene names: 108758026

Gene position: 6521651-6520839 (Counterclockwise)

Preceding gene: 108756792

Following gene: 108763118

Centisome position: 71.35

GC content: 68.39

Gene sequence:

>813_bases
GTGCGCTTCGTTCTGCAGATCTGGAGACAGGAGAGGCCGCGGTCCCTGGGCGCCTTCCGCCGTTACGAGGTGTCTGACAT
CACCGCCGACATGTCCTTTCTCGAGATGCTCGATGTCCTGAACGAGCAACTCGCCGCCAAAGGCGAGGCCCCGGTCGCGT
TCGAGAGCGACTGTCGCGAGGGCATCTGCGGGACGTGCGGCGTGATGATCAATGGCGCGGCGCATGGTCCCCTCGGCGGC
GCGGCGGTGTGTCAGCTCCACATGCGCCGCTTTCCGGACGGCGCGCTGCTGGTGATCGAGCCCTGGCGCGCGCGGGCGTT
TCCTGTGCTCAAGGATCTCATCGTCGATCGCAGCGCGCTCGACCGAATCATCGGCGCTGGCGGGTTCATCTCGGTGCCAA
CGGGGGCCGCGCCCGAGGCGAGCACGCGGGCCGTCTCGAAACAGAACGCCGACGGGGCGATGGACGCCGCGGCTTGCATC
GGCTGTGGGGCCTGCGTCGCCGCCTGCCCGAACGCCTCGGCCGCGCTCTTCACCGGCGCGAAGGTCTCTCACCTGGCGCT
CCTGCCTCAGGGGCGGCCGGAGCGGGAGCGGCGGGTGCTCTCCATGGTCGCGCGCATGGATGAGGAGGGCTTTGGCAACT
GCACCTGGCACGGCGAGTGCCAGGCGGCGTGCCCGAAGTCGATCAGCATCGATGCCATCGCGCTCATGAACCATGAATGG
CTCCGGGCATCGATCGCATCCCAACGCGAAGTGTGGCGCGAGCCTGAGGCGGACGCCCAGAGCCAAGACCCGGAAAGAGG
AGGAACGGCATGA

Upstream 100 bases:

>100_bases
CCGCGCGGCGGCGCATCCGTGATGGGCAATCTGCTGAACACCCGCGCGACGAGTACCTCCATACCGAGGCGCTCGTGGGC
GAAGGGGGAAGGAGAACAGC

Downstream 100 bases:

>100_bases
CCCAACCGATACATCCCGTAGGCGGAGCAACGTTCGACCTGGCGGCAGTCGAGCGTGAAATGCGGCGAGAAGACGCCTAC
CAGCGGGAGGGACACACGGC

Product: succinate dehydrogenase/fumarate reductase iron-sulfur subunit

Products: fumarate; reduced acceptor

Alternate protein names: NA

Number of amino acids: Translated: 270; Mature: 270

Protein sequence:

>270_residues
MRFVLQIWRQERPRSLGAFRRYEVSDITADMSFLEMLDVLNEQLAAKGEAPVAFESDCREGICGTCGVMINGAAHGPLGG
AAVCQLHMRRFPDGALLVIEPWRARAFPVLKDLIVDRSALDRIIGAGGFISVPTGAAPEASTRAVSKQNADGAMDAAACI
GCGACVAACPNASAALFTGAKVSHLALLPQGRPERERRVLSMVARMDEEGFGNCTWHGECQAACPKSISIDAIALMNHEW
LRASIASQREVWREPEADAQSQDPERGGTA

Sequences:

>Translated_270_residues
MRFVLQIWRQERPRSLGAFRRYEVSDITADMSFLEMLDVLNEQLAAKGEAPVAFESDCREGICGTCGVMINGAAHGPLGG
AAVCQLHMRRFPDGALLVIEPWRARAFPVLKDLIVDRSALDRIIGAGGFISVPTGAAPEASTRAVSKQNADGAMDAAACI
GCGACVAACPNASAALFTGAKVSHLALLPQGRPERERRVLSMVARMDEEGFGNCTWHGECQAACPKSISIDAIALMNHEW
LRASIASQREVWREPEADAQSQDPERGGTA
>Mature_270_residues
MRFVLQIWRQERPRSLGAFRRYEVSDITADMSFLEMLDVLNEQLAAKGEAPVAFESDCREGICGTCGVMINGAAHGPLGG
AAVCQLHMRRFPDGALLVIEPWRARAFPVLKDLIVDRSALDRIIGAGGFISVPTGAAPEASTRAVSKQNADGAMDAAACI
GCGACVAACPNASAALFTGAKVSHLALLPQGRPERERRVLSMVARMDEEGFGNCTWHGECQAACPKSISIDAIALMNHEW
LRASIASQREVWREPEADAQSQDPERGGTA

Specific function: Two Distinct, Membrane-Bound, FAD-Containing Enzymes Are Responsible For The Catalysis Of Fumarate And Succinate Interconversion; The Fumarate Reductase Is Used In Anaerobic Growth, And The Succinate Dehydrogenase Is Used In Aerobic Growth. [C]

COG id: COG0479

COG function: function code C; Succinate dehydrogenase/fumarate reductase, Fe-S protein subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1790596, Length=208, Percent_Identity=25.4807692307692, Blast_Score=65, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006058
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR012675
- InterPro:   IPR004017
- InterPro:   IPR001041
- InterPro:   IPR012285
- InterPro:   IPR009051
- InterPro:   IPR004489 [H]

Pfam domain/function: PF02754 CCG; PF00111 Fer2 [H]

EC number: 1.3.99.1

Molecular weight: Translated: 28950; Mature: 28950

Theoretical pI: Translated: 5.70; Mature: 5.70

Prosite motif: PS00197 2FE2S_FER_1 ; PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.1 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
7.4 %Cys+Met (Translated Protein)
4.1 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
7.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFVLQIWRQERPRSLGAFRRYEVSDITADMSFLEMLDVLNEQLAAKGEAPVAFESDCRE
CHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHC
GICGTCGVMINGAAHGPLGGAAVCQLHMRRFPDGALLVIEPWRARAFPVLKDLIVDRSAL
CCCCCCCEEEECCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHCHHHH
DRIIGAGGFISVPTGAAPEASTRAVSKQNADGAMDAAACIGCGACVAACPNASAALFTGA
HHHHCCCCEEECCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECC
KVSHLALLPQGRPERERRVLSMVARMDEEGFGNCTWHGECQAACPKSISIDAIALMNHEW
CCEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCEECCCCCCCCCCCCCCCEEEHHCHHH
LRASIASQREVWREPEADAQSQDPERGGTA
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MRFVLQIWRQERPRSLGAFRRYEVSDITADMSFLEMLDVLNEQLAAKGEAPVAFESDCRE
CHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHC
GICGTCGVMINGAAHGPLGGAAVCQLHMRRFPDGALLVIEPWRARAFPVLKDLIVDRSAL
CCCCCCCEEEECCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHCHHHH
DRIIGAGGFISVPTGAAPEASTRAVSKQNADGAMDAAACIGCGACVAACPNASAALFTGA
HHHHCCCCEEECCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECC
KVSHLALLPQGRPERERRVLSMVARMDEEGFGNCTWHGECQAACPKSISIDAIALMNHEW
CCEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCEECCCCCCCCCCCCCCCEEEHHCHHH
LRASIASQREVWREPEADAQSQDPERGGTA
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: ATP; Dithiothreitol; FAD; Fe-S-clusters [C]

Metal ions: Anions; Diphosphate; Fe [C]

Kcat value (1/min): 10000-13000 [C]

Specific activity: 509

Km value (mM): 0.4 {fumarate}} 0.02 {succinate}} 0.3 {ferricyanide}} [C]

Substrates: succinate; acceptor

Specific reaction: succinate + acceptor = fumarate + reduced acceptor

General reaction: Redox reaction [C]

Inhibitor: 5, 5'-Dithiobis (2-nitrobenzoate); Chaotropic reagents [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA