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The map label for this gene is cspB [H]

Identifier: 108757963

GI number: 108757963

Start: 6613968

End: 6614168

Strand: Reverse

Name: cspB [H]

Synonym: MXAN_5310

Alternate gene names: 108757963

Gene position: 6614168-6613968 (Counterclockwise)

Preceding gene: 108764060

Following gene: 108761656

Centisome position: 72.37

GC content: 62.19

Gene sequence:

>201_bases
ATGGCAATCGGTACCGTGAAGTGGTTCAACGACGCCAAGGGGTTCGGATTCATCGCGCAGGACAACGGTGAGGACGTGTT
CTGCCACCACACTGCCATCAACATGGATGGCTTCCGCACCCTCCAGGAAGGGCAGCAGGTGGAGTTCGAGGTCACGCGTG
GCCCCAAGGGCCTGCAGGCGCAGAACGTCCGCGCCGTCTGA

Upstream 100 bases:

>100_bases
CCAGGAAGTTTGTCGTTAGGGGGCCGGGGCGGTGTCCGCTCCGCACACGTCGATCGGGCAGCCCTTGGCGGCGGCCTCGA
AGCAGAAGGAAGAAGGTCAC

Downstream 100 bases:

>100_bases
CGCACCCGCAGGCCACTTCCGAGTGTGCCCGAGGCCCGGTCTCCCAGCAGGGAGGCGGGGCCTTCGCATGTCTGGCTCCT
CGTGCGCCGGGCTGGCGGCC

Product: cold-shock protein CspB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 66; Mature: 65

Protein sequence:

>66_residues
MAIGTVKWFNDAKGFGFIAQDNGEDVFCHHTAINMDGFRTLQEGQQVEFEVTRGPKGLQAQNVRAV

Sequences:

>Translated_66_residues
MAIGTVKWFNDAKGFGFIAQDNGEDVFCHHTAINMDGFRTLQEGQQVEFEVTRGPKGLQAQNVRAV
>Mature_65_residues
AIGTVKWFNDAKGFGFIAQDNGEDVFCHHTAINMDGFRTLQEGQQVEFEVTRGPKGLQAQNVRAV

Specific function: Inhibits DNA Replication At Both Initiation And Elongation Steps, Most Probably By Binding To The Opened, Single- Stranded Regions At Replication Forks. Plays A Regulatory Role In Chromosomal Replication In Nutrient-Depleted Cells. [C]

COG id: COG1278

COG function: function code K; Cold shock proteins

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CSD (cold-shock) domain [H]

Homologues:

Organism=Escherichia coli, GI1787107, Length=67, Percent_Identity=56.7164179104478, Blast_Score=81, Evalue=2e-17,
Organism=Escherichia coli, GI1786841, Length=64, Percent_Identity=59.375, Blast_Score=80, Evalue=3e-17,
Organism=Escherichia coli, GI1788126, Length=64, Percent_Identity=57.8125, Blast_Score=73, Evalue=3e-15,
Organism=Escherichia coli, GI1789979, Length=64, Percent_Identity=53.125, Blast_Score=71, Evalue=2e-14,
Organism=Escherichia coli, GI2367114, Length=61, Percent_Identity=57.3770491803279, Blast_Score=69, Evalue=6e-14,
Organism=Escherichia coli, GI1787834, Length=64, Percent_Identity=53.125, Blast_Score=68, Evalue=1e-13,
Organism=Escherichia coli, GI1787839, Length=61, Percent_Identity=55.7377049180328, Blast_Score=67, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24658520, Length=64, Percent_Identity=48.4375, Blast_Score=66, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019844
- InterPro:   IPR012156
- InterPro:   IPR011129
- InterPro:   IPR002059
- InterPro:   IPR012340
- InterPro:   IPR016027 [H]

Pfam domain/function: PF00313 CSD [H]

EC number: NA

Molecular weight: Translated: 7327; Mature: 7196

Theoretical pI: Translated: 5.58; Mature: 5.58

Prosite motif: PS00352 COLD_SHOCK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIGTVKWFNDAKGFGFIAQDNGEDVFCHHTAINMDGFRTLQEGQQVEFEVTRGPKGLQA
CCCCCEEECCCCCCCEEEEECCCCEEEEEEEEECCCCCEECCCCCEEEEEECCCCCCCCC
QNVRAV
CCEECC
>Mature Secondary Structure 
AIGTVKWFNDAKGFGFIAQDNGEDVFCHHTAINMDGFRTLQEGQQVEFEVTRGPKGLQA
CCCCEEECCCCCCCEEEEECCCCEEEEEEEEECCCCCEECCCCCEEEEEECCCCCCCCC
QNVRAV
CCEECC

PDB accession: NA

Resolution: NA

Structure class: Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA