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The map label for this gene is lipB

Identifier: 108757734

GI number: 108757734

Start: 5174006

End: 5175079

Strand: Reverse

Name: lipB

Synonym: MXAN_4215

Alternate gene names: 108757734

Gene position: 5175079-5174006 (Counterclockwise)

Preceding gene: 108758514

Following gene: 108762555

Centisome position: 56.62

GC content: 69.74

Gene sequence:

>1074_bases
ATGAACACCATCACCGTCTACCGCCTGGGCCGAGTGGAGTACGAGGACGGGCTCGCGCTGATGCACCTCTTCAGCGAGTC
GCGCCGCCAGGGGCTCTCCGGCGATGTGCTGCTCCTCTTGGAGCACCCGCCCATCCTCACCCTGGGCCGTGCCGCGAAGC
GTGAGAACATCGTCGCCAGCGACGCGCAGTTGGCGAAGGAGGGCGCGGAGGTCTTCGAGACCAACCGTGGCGGCGACGTC
ACCTACCATGGCCCCGGCCAACTGGTGGGCTACCCCATCTTCCTGCTGCCGGAGGACCGACGCGACGTGCGCCGTTACGT
GCGCGACGTGGAGCGCTCCGTCATGCAGGTGCTGGCCCAGTGGGGCATCACCGCGGGCCCCATCCCCAAGTGGCCCGGCG
TCTGGATTGGCGCGGAAGGTGCTCCGGACGCGCGGAAGATCGCCGCCATTGGCGTGCACCTGTCGCGTTGGCTCACCACG
CACGGCTTCGCGCTCAACGTGAACACGAACCTGGACCACTTCCAGCTCATCGTCCCGTGTGGCATCCGAGAAGCAGGTGT
CACGTCCATGCAGCGGGAGTTGGGCCGCGCGCTCCCCATGGCGGAGGTGGAAGAGGCCATCGCCAACAGCTTCTGCACCG
TGTTCGACAGCGAGCGCGTGGATGCGCCGCCGCCGATGCGGACGGTGAGCATCGCGGTGGTGAAGGGCCGTGGTCCCGAA
GCACGGGTGCTGCTCGTGCGCCGTCGCCCGGAGCGGGGCGGTTTCTGGCAGGTCCTCACCGGGAGGCTCGAAGCGGGTGA
GTCGCCAGCGCAGGCCGCGGCGCGGGAACTGGAGGAGGAGACAGGCCTCCGCGTGCCGCTGGTGGACCTGGACTACCGCC
ACGCCTTCGCCCTGGGAGAGGCGCTGCCACCGCAACTCGTGGAGGAGAACGGCTTCGCCGTCCACGTGCCGCCGGACGCG
GACGTGCGCCTGGGCGCGGAGCACGACGCCTTCGAGTGGGTGGACGTCCCCACGGCCCTGGAGCGGCTGCCCTTCCAGGG
CCTGCGGGAGACGGTGAAGCGCGCTACAGCTTGA

Upstream 100 bases:

>100_bases
AGCTGCATGCCCTCCTCAACCGGGTACCCACGGGAACGTGGAGCCTGGCGCCCACCGTGAAGCCCTCGCCCACGCGGAAG
GCTCGAAAGAAGCGAACGTC

Downstream 100 bases:

>100_bases
TGACCAGGGACTCGCGAGCGGCGATGGCGTCGGGGCGGTGCTTGCGCACCTGACGCAGCAGCCGGTCCATTCCCGCGTCA
TCTCGTGACGGGTCGTGGTG

Product: lipoate-protein ligase B

Products: NA

Alternate protein names: Lipoate-protein ligase B; Lipoyl/octanoyl transferase; Octanoyl-[acyl-carrier-protein]-protein N-octanoyltransferase

Number of amino acids: Translated: 357; Mature: 357

Protein sequence:

>357_residues
MNTITVYRLGRVEYEDGLALMHLFSESRRQGLSGDVLLLLEHPPILTLGRAAKRENIVASDAQLAKEGAEVFETNRGGDV
TYHGPGQLVGYPIFLLPEDRRDVRRYVRDVERSVMQVLAQWGITAGPIPKWPGVWIGAEGAPDARKIAAIGVHLSRWLTT
HGFALNVNTNLDHFQLIVPCGIREAGVTSMQRELGRALPMAEVEEAIANSFCTVFDSERVDAPPPMRTVSIAVVKGRGPE
ARVLLVRRRPERGGFWQVLTGRLEAGESPAQAAARELEEETGLRVPLVDLDYRHAFALGEALPPQLVEENGFAVHVPPDA
DVRLGAEHDAFEWVDVPTALERLPFQGLRETVKRATA

Sequences:

>Translated_357_residues
MNTITVYRLGRVEYEDGLALMHLFSESRRQGLSGDVLLLLEHPPILTLGRAAKRENIVASDAQLAKEGAEVFETNRGGDV
TYHGPGQLVGYPIFLLPEDRRDVRRYVRDVERSVMQVLAQWGITAGPIPKWPGVWIGAEGAPDARKIAAIGVHLSRWLTT
HGFALNVNTNLDHFQLIVPCGIREAGVTSMQRELGRALPMAEVEEAIANSFCTVFDSERVDAPPPMRTVSIAVVKGRGPE
ARVLLVRRRPERGGFWQVLTGRLEAGESPAQAAARELEEETGLRVPLVDLDYRHAFALGEALPPQLVEENGFAVHVPPDA
DVRLGAEHDAFEWVDVPTALERLPFQGLRETVKRATA
>Mature_357_residues
MNTITVYRLGRVEYEDGLALMHLFSESRRQGLSGDVLLLLEHPPILTLGRAAKRENIVASDAQLAKEGAEVFETNRGGDV
TYHGPGQLVGYPIFLLPEDRRDVRRYVRDVERSVMQVLAQWGITAGPIPKWPGVWIGAEGAPDARKIAAIGVHLSRWLTT
HGFALNVNTNLDHFQLIVPCGIREAGVTSMQRELGRALPMAEVEEAIANSFCTVFDSERVDAPPPMRTVSIAVVKGRGPE
ARVLLVRRRPERGGFWQVLTGRLEAGESPAQAAARELEEETGLRVPLVDLDYRHAFALGEALPPQLVEENGFAVHVPPDA
DVRLGAEHDAFEWVDVPTALERLPFQGLRETVKRATA

Specific function: Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domains of lipoate-dependent enzymes. Lipoyl-ACP can also act as a substrate although octanoyl-ACP is likely to be the physiological substrate

COG id: COG0321

COG function: function code H; Lipoate-protein ligase B

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 nudix hydrolase domain

Homologues:

Organism=Homo sapiens, GI221554520, Length=235, Percent_Identity=35.3191489361702, Blast_Score=103, Evalue=3e-22,
Organism=Escherichia coli, GI87081767, Length=180, Percent_Identity=38.3333333333333, Blast_Score=110, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6323268, Length=177, Percent_Identity=27.683615819209, Blast_Score=73, Evalue=6e-14,
Organism=Drosophila melanogaster, GI21355483, Length=219, Percent_Identity=41.0958904109589, Blast_Score=155, Evalue=4e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LIPB_MYXXA (Q9X6X4)

Other databases:

- EMBL:   AF153678
- ProteinModelPortal:   Q9X6X4
- BRENDA:   2.3.1.181
- GO:   GO:0005737
- HAMAP:   MF_00013
- InterPro:   IPR004143
- InterPro:   IPR020476
- InterPro:   IPR020084
- InterPro:   IPR000086
- InterPro:   IPR015797
- InterPro:   IPR000544
- InterPro:   IPR020605
- Gene3D:   G3DSA:3.90.79.10
- PRINTS:   PR00502
- TIGRFAMs:   TIGR00214

Pfam domain/function: PF03099 BPL_LipA_LipB; PF00293 NUDIX; SSF55811 NUDIX_hydrolase

EC number: =2.3.1.181

Molecular weight: Translated: 39373; Mature: 39373

Theoretical pI: Translated: 5.66; Mature: 5.66

Prosite motif: PS01313 LIPB; PS51462 NUDIX; PS00893 NUDIX_BOX

Important sites: ACT_SITE 180-180

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTITVYRLGRVEYEDGLALMHLFSESRRQGLSGDVLLLLEHPPILTLGRAAKRENIVAS
CCCEEEEEECCEECCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCEEECCHHHHCCCCCC
DAQLAKEGAEVFETNRGGDVTYHGPGQLVGYPIFLLPEDRRDVRRYVRDVERSVMQVLAQ
HHHHHHHHHHHHCCCCCCCEEECCCCCEECEEEEEECCCHHHHHHHHHHHHHHHHHHHHH
WGITAGPIPKWPGVWIGAEGAPDARKIAAIGVHLSRWLTTHGFALNVNTNLDHFQLIVPC
CCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHCCEEEEECCCCCEEEEEEEC
GIREAGVTSMQRELGRALPMAEVEEAIANSFCTVFDSERVDAPPPMRTVSIAVVKGRGPE
CCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEECCCCC
ARVLLVRRRPERGGFWQVLTGRLEAGESPAQAAARELEEETGLRVPLVDLDYRHAFALGE
EEEEEEEECCCCCCCHHHEECHHCCCCCHHHHHHHHHHHHCCCEEEEEECCCHHHHHHCC
ALPPQLVEENGFAVHVPPDADVRLGAEHDAFEWVDVPTALERLPFQGLRETVKRATA
CCCHHHHCCCCCEEECCCCCCEEECCCCCCCEEECCHHHHHHCCHHHHHHHHHHHCC
>Mature Secondary Structure
MNTITVYRLGRVEYEDGLALMHLFSESRRQGLSGDVLLLLEHPPILTLGRAAKRENIVAS
CCCEEEEEECCEECCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCEEECCHHHHCCCCCC
DAQLAKEGAEVFETNRGGDVTYHGPGQLVGYPIFLLPEDRRDVRRYVRDVERSVMQVLAQ
HHHHHHHHHHHHCCCCCCCEEECCCCCEECEEEEEECCCHHHHHHHHHHHHHHHHHHHHH
WGITAGPIPKWPGVWIGAEGAPDARKIAAIGVHLSRWLTTHGFALNVNTNLDHFQLIVPC
CCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHCCEEEEECCCCCEEEEEEEC
GIREAGVTSMQRELGRALPMAEVEEAIANSFCTVFDSERVDAPPPMRTVSIAVVKGRGPE
CCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEECCCCC
ARVLLVRRRPERGGFWQVLTGRLEAGESPAQAAARELEEETGLRVPLVDLDYRHAFALGE
EEEEEEEECCCCCCCHHHEECHHCCCCCHHHHHHHHHHHHCCCEEEEEECCCHHHHHHCC
ALPPQLVEENGFAVHVPPDADVRLGAEHDAFEWVDVPTALERLPFQGLRETVKRATA
CCCHHHHCCCCCEEECCCCCCEEECCCCCCCEEECCHHHHHHCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA