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The map label for this gene is thiC

Identifier: 108757595

GI number: 108757595

Start: 5194388

End: 5196262

Strand: Reverse

Name: thiC

Synonym: MXAN_4235

Alternate gene names: 108757595

Gene position: 5196262-5194388 (Counterclockwise)

Preceding gene: 108757728

Following gene: 108758672

Centisome position: 56.85

GC content: 67.52

Gene sequence:

>1875_bases
ATGAGCGGAGCGTCCAGAAGCCTGAAGGTCGACGGGAAGGTGCTGGAGGGAATCAGCCGGGGCCCGCTGCCAGCCTCGCG
CAAGGTGTACGTGCCCGGCGTGCTGCACCCGGACCTGCGCGTGCCCCTGCGCGAAATCAGCCAGACGCCCACGCGCCTCG
GCCACGGCCCGGACGCGAAGGAGACGGCCAACCCGCCGGTGCACGTCTACGACTCCAGCGGGCCGTATACGGATCCGGCC
GCGGAGCTCGACCTGCGGCAGGGGCTGCCCGCGCTGCGCGAGTCCTGGATCCTGGGCCGCAATGACACCGAAGCGCTGTC
CGGCGTGAGCTCCGAATACGGCCGCGCCCGTGAAGCGGACCCGCGTCTGGCGGGCCTGCGCTTCGCCCACCGCCGCTCGC
CGCGCGTGGCGAAGTCCGGCGCCAACGTCACGCAGTTGCACTACGCGCGCAAGGGCATCATCACCCCGGAGATGGAGTAC
GTGGCGCTGCGGGAGAATCTCCAGGTGGAGGCGTCGCTCGCGGCCCAGCACCCGGGCCAGTCGTGGGGCGCGGCGATTCC
GCGGGTGATTACGCCCGAGTTCGTGCGCGACGAAATCGCGCGGGGCCGCGCCATCATCCCGGCCAACATCAACCACCCGG
AGCTGGAGCCGATGATCATCGGCCGCAACTTCCTGGTGAAGATCAACGCCAACATCGGCAACTCGGCCGTCACGTCTTCC
ATCGAGGAAGAGGTGGAGAAGATGGTGTGGTCCATCCGCTGGGGCGCGGACACGGTGATGGACCTGTCCACCGGCCGAAA
CATCCACGAGACGCGCGAGTGGATCCTCCGCAACGCGCCGGTGCCCATCGGCACCGTGCCCATCTACCAGGCGCTGGAGA
AGGTGGGCGGCAAGGCGGAGGACCTCACCTGGGAGCTGTACCGCGACACGCTCATCGAGCAGTGCGAGCAGGGCGTGGAT
TACTTCACCATCCACGCGGGCGTGCTGCTGCGCTACGTGCCGTGGACGGCGAAGCGCCTGACCGGCATCGTCAGCCGTGG
CGGCTCCATCATGGCCAAGTGGTGCCTCGCCCACCACCGGGAGAACTTCCTCTACACGCACTTCGAGGAAATCTGCGAAA
TCATGAAGGCGTACGACGTCAGCTTCAGCCTGGGAGACGGGCTGCGGCCGGGCTCCATCGCGGATGCCAACGACGCGGCG
CAGTTCGGTGAGCTGGAGACGCTGGGCGAGCTCACCCAGATTGCCTGGAAGCACGACGTGCAGGTGATGATTGAAGGCCC
GGGCCACGTGCCCATGCACCTCATCCAGGAGAACATGACGAAGCAGCTGGCCGTGTGCGGCGAGGCGCCGTTCTACACGC
TGGGGCCTCTCACCACGGACATCGCGCCGGGCTACGACCACTTCACCAGCGGCATCGGCGCGGCGATGATCGGCTGGTTC
GGCACGGCGATGCTCTGCTACGTGACGCCCAAGGAGCACCTGGGCCTGCCGGATCGGGACGACGTGAAGGAAGGCGTCAT
CACGTACAAGATTGCCGCCCACGCCGCGGACCTGGCCAAGGGGCATCCAGGGGCCCAGGCCCGGGACAACGCGCTGTCCA
AGGCCCGCTTCGAGTTCCGTTGGGAGGACCAGTTCAACCTGTCGCTGGACCCCGAGCGCGCCCGCGCCTTCCACGACGAG
ACGCTTCCGGCGGAAGGCGCGAAGGTGGCGCACTTCTGCTCCATGTGCGGTCCGCAGTTCTGCTCCATGAAGATCACGCA
AGAGGTGCGTGACTTCGCGGCGAAGCAGGGCGTGAGCGACGACACCGCGCTTCAGTCCGCGCTCGATGAGAAGAGCGCGG
AATTCAAGAAGGCTGGGAGCCAGTTGTACCGCTAG

Upstream 100 bases:

>100_bases
TTGGAACCTGAACCGGTTCGTACCGGCGGAGGGAAGCGGCCCGGGAGGCTTGCTGCCCCCCGACCGCCTGCCCGTCCGCC
CTCTCAAGAGGAGATGCGTG

Downstream 100 bases:

>100_bases
GCGGGTGCCCCTTCAGATCACCTGTCCTGGACGTGACAATCAGAGCGGGTGCGTGTCATGGTGGACCCCGCTGGGTGCGG
TGCACGCGCCCGAGACATCT

Product: thiamine biosynthesis protein ThiC

Products: @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine!; 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

Alternate protein names: Hydroxymethylpyrimidine phosphate synthase; HMP-P synthase; HMP-phosphate synthase; HMPP synthase; Thiamine biosynthesis protein thiC

Number of amino acids: Translated: 624; Mature: 623

Protein sequence:

>624_residues
MSGASRSLKVDGKVLEGISRGPLPASRKVYVPGVLHPDLRVPLREISQTPTRLGHGPDAKETANPPVHVYDSSGPYTDPA
AELDLRQGLPALRESWILGRNDTEALSGVSSEYGRAREADPRLAGLRFAHRRSPRVAKSGANVTQLHYARKGIITPEMEY
VALRENLQVEASLAAQHPGQSWGAAIPRVITPEFVRDEIARGRAIIPANINHPELEPMIIGRNFLVKINANIGNSAVTSS
IEEEVEKMVWSIRWGADTVMDLSTGRNIHETREWILRNAPVPIGTVPIYQALEKVGGKAEDLTWELYRDTLIEQCEQGVD
YFTIHAGVLLRYVPWTAKRLTGIVSRGGSIMAKWCLAHHRENFLYTHFEEICEIMKAYDVSFSLGDGLRPGSIADANDAA
QFGELETLGELTQIAWKHDVQVMIEGPGHVPMHLIQENMTKQLAVCGEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWF
GTAMLCYVTPKEHLGLPDRDDVKEGVITYKIAAHAADLAKGHPGAQARDNALSKARFEFRWEDQFNLSLDPERARAFHDE
TLPAEGAKVAHFCSMCGPQFCSMKITQEVRDFAAKQGVSDDTALQSALDEKSAEFKKAGSQLYR

Sequences:

>Translated_624_residues
MSGASRSLKVDGKVLEGISRGPLPASRKVYVPGVLHPDLRVPLREISQTPTRLGHGPDAKETANPPVHVYDSSGPYTDPA
AELDLRQGLPALRESWILGRNDTEALSGVSSEYGRAREADPRLAGLRFAHRRSPRVAKSGANVTQLHYARKGIITPEMEY
VALRENLQVEASLAAQHPGQSWGAAIPRVITPEFVRDEIARGRAIIPANINHPELEPMIIGRNFLVKINANIGNSAVTSS
IEEEVEKMVWSIRWGADTVMDLSTGRNIHETREWILRNAPVPIGTVPIYQALEKVGGKAEDLTWELYRDTLIEQCEQGVD
YFTIHAGVLLRYVPWTAKRLTGIVSRGGSIMAKWCLAHHRENFLYTHFEEICEIMKAYDVSFSLGDGLRPGSIADANDAA
QFGELETLGELTQIAWKHDVQVMIEGPGHVPMHLIQENMTKQLAVCGEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWF
GTAMLCYVTPKEHLGLPDRDDVKEGVITYKIAAHAADLAKGHPGAQARDNALSKARFEFRWEDQFNLSLDPERARAFHDE
TLPAEGAKVAHFCSMCGPQFCSMKITQEVRDFAAKQGVSDDTALQSALDEKSAEFKKAGSQLYR
>Mature_623_residues
SGASRSLKVDGKVLEGISRGPLPASRKVYVPGVLHPDLRVPLREISQTPTRLGHGPDAKETANPPVHVYDSSGPYTDPAA
ELDLRQGLPALRESWILGRNDTEALSGVSSEYGRAREADPRLAGLRFAHRRSPRVAKSGANVTQLHYARKGIITPEMEYV
ALRENLQVEASLAAQHPGQSWGAAIPRVITPEFVRDEIARGRAIIPANINHPELEPMIIGRNFLVKINANIGNSAVTSSI
EEEVEKMVWSIRWGADTVMDLSTGRNIHETREWILRNAPVPIGTVPIYQALEKVGGKAEDLTWELYRDTLIEQCEQGVDY
FTIHAGVLLRYVPWTAKRLTGIVSRGGSIMAKWCLAHHRENFLYTHFEEICEIMKAYDVSFSLGDGLRPGSIADANDAAQ
FGELETLGELTQIAWKHDVQVMIEGPGHVPMHLIQENMTKQLAVCGEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFG
TAMLCYVTPKEHLGLPDRDDVKEGVITYKIAAHAADLAKGHPGAQARDNALSKARFEFRWEDQFNLSLDPERARAFHDET
LPAEGAKVAHFCSMCGPQFCSMKITQEVRDFAAKQGVSDDTALQSALDEKSAEFKKAGSQLYR

Specific function: Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction

COG id: COG0422

COG function: function code H; Thiamine biosynthesis protein ThiC

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiC family

Homologues:

Organism=Escherichia coli, GI1790427, Length=615, Percent_Identity=71.219512195122, Blast_Score=905, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIC_MYXXD (Q1D4L3)

Other databases:

- EMBL:   CP000113
- RefSeq:   YP_632410.1
- STRING:   Q1D4L3
- GeneID:   4105256
- GenomeReviews:   CP000113_GR
- KEGG:   mxa:MXAN_4235
- TIGR:   MXAN_4235
- eggNOG:   COG0422
- HOGENOM:   HBG285234
- OMA:   TWELFRD
- PhylomeDB:   Q1D4L3
- ProtClustDB:   PRK09284
- BioCyc:   MXAN246197:MXAN_4235-MONOMER
- HAMAP:   MF_00089
- InterPro:   IPR002817
- TIGRFAMs:   TIGR00190

Pfam domain/function: PF01964 ThiC

EC number: NA

Molecular weight: Translated: 68785; Mature: 68654

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: PS00133 CARBOXYPEPT_ZN_2

Important sites: BINDING 231-231 BINDING 260-260 BINDING 289-289 BINDING 325-325 BINDING 425-425 BINDING 452-452

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGASRSLKVDGKVLEGISRGPLPASRKVYVPGVLHPDLRVPLREISQTPTRLGHGPDAK
CCCCCCCEEECHHHHHCCCCCCCCCCCEEEECCCCCCCCCCCHHHHHCCHHHCCCCCCCC
ETANPPVHVYDSSGPYTDPAAELDLRQGLPALRESWILGRNDTEALSGVSSEYGRAREAD
CCCCCCEEEECCCCCCCCCHHHHHHHHCCHHHHHCEECCCCCHHHHHCCHHHHCCCCCCC
PRLAGLRFAHRRSPRVAKSGANVTQLHYARKGIITPEMEYVALRENLQVEASLAAQHPGQ
CHHHHHHHHHHCCCCHHHCCCCHHHHHHHHCCCCCCCHHHHHHHHCCEEEEHHHHCCCCC
SWGAAIPRVITPEFVRDEIARGRAIIPANINHPELEPMIIGRNFLVKINANIGNSAVTSS
CHHCCCCCCCCHHHHHHHHHCCCEEEECCCCCCCCCEEEECCEEEEEEECCCCCHHHHHH
IEEEVEKMVWSIRWGADTVMDLSTGRNIHETREWILRNAPVPIGTVPIYQALEKVGGKAE
HHHHHHHHHHHHCCCCCCEEECCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCC
DLTWELYRDTLIEQCEQGVDYFTIHAGVLLRYVPWTAKRLTGIVSRGGSIMAKWCLAHHR
CHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHH
ENFLYTHFEEICEIMKAYDVSFSLGDGLRPGSIADANDAAQFGELETLGELTQIAWKHDV
CCCHHHHHHHHHHHHHHHCCCEECCCCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHCCC
QVMIEGPGHVPMHLIQENMTKQLAVCGEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWF
EEEEECCCCCHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHH
GTAMLCYVTPKEHLGLPDRDDVKEGVITYKIAAHAADLAKGHPGAQARDNALSKARFEFR
HHEEEEEECCHHHCCCCCHHHHHCCCEEEEHHHHHHHHHCCCCCCCHHHHHHHHCEEEEE
WEDQFNLSLDPERARAFHDETLPAEGAKVAHFCSMCGPQFCSMKITQEVRDFAAKQGVSD
ECCCCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCH
DTALQSALDEKSAEFKKAGSQLYR
HHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SGASRSLKVDGKVLEGISRGPLPASRKVYVPGVLHPDLRVPLREISQTPTRLGHGPDAK
CCCCCCEEECHHHHHCCCCCCCCCCCEEEECCCCCCCCCCCHHHHHCCHHHCCCCCCCC
ETANPPVHVYDSSGPYTDPAAELDLRQGLPALRESWILGRNDTEALSGVSSEYGRAREAD
CCCCCCEEEECCCCCCCCCHHHHHHHHCCHHHHHCEECCCCCHHHHHCCHHHHCCCCCCC
PRLAGLRFAHRRSPRVAKSGANVTQLHYARKGIITPEMEYVALRENLQVEASLAAQHPGQ
CHHHHHHHHHHCCCCHHHCCCCHHHHHHHHCCCCCCCHHHHHHHHCCEEEEHHHHCCCCC
SWGAAIPRVITPEFVRDEIARGRAIIPANINHPELEPMIIGRNFLVKINANIGNSAVTSS
CHHCCCCCCCCHHHHHHHHHCCCEEEECCCCCCCCCEEEECCEEEEEEECCCCCHHHHHH
IEEEVEKMVWSIRWGADTVMDLSTGRNIHETREWILRNAPVPIGTVPIYQALEKVGGKAE
HHHHHHHHHHHHCCCCCCEEECCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCC
DLTWELYRDTLIEQCEQGVDYFTIHAGVLLRYVPWTAKRLTGIVSRGGSIMAKWCLAHHR
CHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHH
ENFLYTHFEEICEIMKAYDVSFSLGDGLRPGSIADANDAAQFGELETLGELTQIAWKHDV
CCCHHHHHHHHHHHHHHHCCCEECCCCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHCCC
QVMIEGPGHVPMHLIQENMTKQLAVCGEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWF
EEEEECCCCCHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHH
GTAMLCYVTPKEHLGLPDRDDVKEGVITYKIAAHAADLAKGHPGAQARDNALSKARFEFR
HHEEEEEECCHHHCCCCCHHHHHCCCEEEEHHHHHHHHHCCCCCCCHHHHHHHHCEEEEE
WEDQFNLSLDPERARAFHDETLPAEGAKVAHFCSMCGPQFCSMKITQEVRDFAAKQGVSD
ECCCCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCH
DTALQSALDEKSAEFKKAGSQLYR
HHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ?; ?; 5'-phosphoribosyl-5-aminoimidazole [C]

Specific reaction: Synthesis of @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine! 5'-phosphoribosyl-5-aminoimidazole = 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA