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The map label for this gene is glgX [H]

Identifier: 108757366

GI number: 108757366

Start: 1801848

End: 1803989

Strand: Direct

Name: glgX [H]

Synonym: MXAN_1534

Alternate gene names: 108757366

Gene position: 1801848-1803989 (Clockwise)

Preceding gene: 108757241

Following gene: 108758817

Centisome position: 19.71

GC content: 66.9

Gene sequence:

>2142_bases
ATGAGGAGGGCCGAGGTGCTTCCAGGGAAGCCGTATCCCCTGGGCGCCACGTTCGATGGGCAGGGTGTCAACTTCGCCGT
TTTCAGCGAGCACGCGAAGAAGGTCGAGGTCTGTCTCTTCGATCCGGAGGACCCCGCGAAGGAGACGCGCCGCTTTCCCT
TGCTGGAGACGACGCACCAGGTGTGGCACGGCTACGTGCCTGGCCTGGCCGCGGGCGCCCTCTACGGACTGCGCGTGCAC
GGGCCCTACGAGCCGAAGAAGGGGCTGCGCTTCAACCCCCACAAGCTGTTGGTGGACCCGTATGCCCGGGCCATCCACGG
CCAGGTGGACTACCAGGCCCCCATCTACGCCTATACGCCGGGCACCAAGGAGGACGACCTCGCGTTCGACACGCGCGATG
ACGCGGCCGCCGTGCCCAAGGGCGTGGTGATGGGCGCGGACACCTTCGACTGGGAGGGAGATACCCCGCCTGGCGTGCCG
TGGCACGACACGCTCATCTATGAAGTCCACGTGAAGGGCTTCACCCAGCTGCACCCGCGCGTCCCGGAGGCGCTGCGGGG
CACCTACGCGGGGCTGGCGCACCCGGCCAGCATCGAGCACCTGAAGAAGGTGGGCGTCACCGCGGTGGAGCTGCTGCCCA
TCCAGCACATCGTCGACGAGCCTTTCCTCATCCAGCGCGAGAAGGTCAATTACTGGGGCTACAACACGCTGGGGTTCTTC
GCGCCGGACGCGCGCTACAGCGGCTCGGGTTCGCTGGGCGGGCAGGTGGACGAGTTCAAGCGCATGGTGAAGCAGCTGCA
CCGCGCTGGCATCGAGGTGCTGCTCGACGTCGTCTACAACCACACCTGCGAAGGCAACCAGCTGGGCCCCACGCTGTCCT
TCAAGGGCGTGGACAACGCGGCGTACTACCGGCTCACGGAGAAGGACCCGCGCTACTACCTGGACGTCACCGGGTGCGGG
AACTCGTGGAACGCCACGCACCCGTACGCGCTGAAGCTCGTCGCCGACTCCCTGCGCTATTGGGTGGAGGAGATGCATGT
GGACGGGTTCCGCTTCGACCTGGCCACCACGCTGGGAAGAGACAGGCACGGCTACGACACCCGCGCCGCCTTCTTCCAAA
TCATCCACCAGGACCCGGTGCTCAGCCGGGTGAAGCTCATCTCCGAACCCTGGGACGTGGGCGACTTTGGCTACCAGGTG
GGCAACTTCCCGGTGCTGTGGAGCGAGTGGAACGGCAAGTACCGCGACACCATCCGCCGCTACTGGAAGGGCGATGACCG
GCAGGCGGCGGAGATTGGCTACCGCCTCACCGGCAGCTCGGACCTGTTCTCACTGTCCGGCCGCAAGCCCGCAGCGAGCG
TGAACTTCGTCACCGCGCACGACGGCTTCACGCTGCACGACCTCGTCACGTACAACGACAAGCACAACGAGGCGAACGGT
GAGGAGAACCGCGACGGCGCCAACGACAACCACTCCTGGAACTGCGGGGTGGAGGGCGAGACGACCGACGCCGAAATCAA
CGCCCTGCGCGAACAGCAGAAGCGCAACTTCCTGGCGACGCTCTTCCTGTCCCAGGGCGTGCCCATGCTGGTGGCCGGCG
ACGAGATGGGCCGCACGCAGAAGGGCAACAACAACGCCTACTGCCAGGACAACGAGCTGTCGTGGGTGGACTGGGAGCTG
AACGACACGCAGCGCGCGCTGCTGGACTTCACCTGCGCCCTGACCAAGCTGCGGCGTGAGCAGCCGGTGCTGCACAAGCG
GCGCTTCTTCCGCGGCGCCCATATGTGGGACAGCGAGCTGAAGGACCTGGCGTGGTTCCGGCCCGATGGCCAGGAGATGC
GCAAGGACGACTGGGAGAAGCCCTACGTGCGCTCCCTGGGATTCCTGCTGGGCGGTGACGCCATCACCGCGCCGGACGAT
GAAGGGAACCGGATTGTGGGGGACACCATCCTGGTGCTGATGAACGCCCACCACGAGCCCATCACCTTCAGGCTGCCTGC
TGTCGAATGGGGCGCGGACTGGGAGCTGGTGGTGGACACGGCGTTGGCGGGGGAGTCGCTTCGCACGCATACGCCGGCGG
GGGGCACCGTGCAGGTGGTGGGCCGCTCCCTGGCGGTGCTGCGCCGCCCGGCGACGGAGTAG

Upstream 100 bases:

>100_bases
GCTGTCAGGTGCGGCCCGAGCGTGGACCGGGTGGTGCGGTGTTGCCTCTGGGGCCGCTCCTGGCGGAGTTTCCGGTGATT
CTGCTGGAGAGGAGCGCCGG

Downstream 100 bases:

>100_bases
CCCCCGCGGGGCCGGAAGGGGCCCGCTGGACGAAAGCAGGAGGTCCAGCGGGTTTCACGCGAGGGGTCGACGCCCGGGCA
GCCCGCCGGTAGGGTGCGCC

Product: glycogen debranching enzyme GlgX

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 713; Mature: 713

Protein sequence:

>713_residues
MRRAEVLPGKPYPLGATFDGQGVNFAVFSEHAKKVEVCLFDPEDPAKETRRFPLLETTHQVWHGYVPGLAAGALYGLRVH
GPYEPKKGLRFNPHKLLVDPYARAIHGQVDYQAPIYAYTPGTKEDDLAFDTRDDAAAVPKGVVMGADTFDWEGDTPPGVP
WHDTLIYEVHVKGFTQLHPRVPEALRGTYAGLAHPASIEHLKKVGVTAVELLPIQHIVDEPFLIQREKVNYWGYNTLGFF
APDARYSGSGSLGGQVDEFKRMVKQLHRAGIEVLLDVVYNHTCEGNQLGPTLSFKGVDNAAYYRLTEKDPRYYLDVTGCG
NSWNATHPYALKLVADSLRYWVEEMHVDGFRFDLATTLGRDRHGYDTRAAFFQIIHQDPVLSRVKLISEPWDVGDFGYQV
GNFPVLWSEWNGKYRDTIRRYWKGDDRQAAEIGYRLTGSSDLFSLSGRKPAASVNFVTAHDGFTLHDLVTYNDKHNEANG
EENRDGANDNHSWNCGVEGETTDAEINALREQQKRNFLATLFLSQGVPMLVAGDEMGRTQKGNNNAYCQDNELSWVDWEL
NDTQRALLDFTCALTKLRREQPVLHKRRFFRGAHMWDSELKDLAWFRPDGQEMRKDDWEKPYVRSLGFLLGGDAITAPDD
EGNRIVGDTILVLMNAHHEPITFRLPAVEWGADWELVVDTALAGESLRTHTPAGGTVQVVGRSLAVLRRPATE

Sequences:

>Translated_713_residues
MRRAEVLPGKPYPLGATFDGQGVNFAVFSEHAKKVEVCLFDPEDPAKETRRFPLLETTHQVWHGYVPGLAAGALYGLRVH
GPYEPKKGLRFNPHKLLVDPYARAIHGQVDYQAPIYAYTPGTKEDDLAFDTRDDAAAVPKGVVMGADTFDWEGDTPPGVP
WHDTLIYEVHVKGFTQLHPRVPEALRGTYAGLAHPASIEHLKKVGVTAVELLPIQHIVDEPFLIQREKVNYWGYNTLGFF
APDARYSGSGSLGGQVDEFKRMVKQLHRAGIEVLLDVVYNHTCEGNQLGPTLSFKGVDNAAYYRLTEKDPRYYLDVTGCG
NSWNATHPYALKLVADSLRYWVEEMHVDGFRFDLATTLGRDRHGYDTRAAFFQIIHQDPVLSRVKLISEPWDVGDFGYQV
GNFPVLWSEWNGKYRDTIRRYWKGDDRQAAEIGYRLTGSSDLFSLSGRKPAASVNFVTAHDGFTLHDLVTYNDKHNEANG
EENRDGANDNHSWNCGVEGETTDAEINALREQQKRNFLATLFLSQGVPMLVAGDEMGRTQKGNNNAYCQDNELSWVDWEL
NDTQRALLDFTCALTKLRREQPVLHKRRFFRGAHMWDSELKDLAWFRPDGQEMRKDDWEKPYVRSLGFLLGGDAITAPDD
EGNRIVGDTILVLMNAHHEPITFRLPAVEWGADWELVVDTALAGESLRTHTPAGGTVQVVGRSLAVLRRPATE
>Mature_713_residues
MRRAEVLPGKPYPLGATFDGQGVNFAVFSEHAKKVEVCLFDPEDPAKETRRFPLLETTHQVWHGYVPGLAAGALYGLRVH
GPYEPKKGLRFNPHKLLVDPYARAIHGQVDYQAPIYAYTPGTKEDDLAFDTRDDAAAVPKGVVMGADTFDWEGDTPPGVP
WHDTLIYEVHVKGFTQLHPRVPEALRGTYAGLAHPASIEHLKKVGVTAVELLPIQHIVDEPFLIQREKVNYWGYNTLGFF
APDARYSGSGSLGGQVDEFKRMVKQLHRAGIEVLLDVVYNHTCEGNQLGPTLSFKGVDNAAYYRLTEKDPRYYLDVTGCG
NSWNATHPYALKLVADSLRYWVEEMHVDGFRFDLATTLGRDRHGYDTRAAFFQIIHQDPVLSRVKLISEPWDVGDFGYQV
GNFPVLWSEWNGKYRDTIRRYWKGDDRQAAEIGYRLTGSSDLFSLSGRKPAASVNFVTAHDGFTLHDLVTYNDKHNEANG
EENRDGANDNHSWNCGVEGETTDAEINALREQQKRNFLATLFLSQGVPMLVAGDEMGRTQKGNNNAYCQDNELSWVDWEL
NDTQRALLDFTCALTKLRREQPVLHKRRFFRGAHMWDSELKDLAWFRPDGQEMRKDDWEKPYVRSLGFLLGGDAITAPDD
EGNRIVGDTILVLMNAHHEPITFRLPAVEWGADWELVVDTALAGESLRTHTPAGGTVQVVGRSLAVLRRPATE

Specific function: Hydrolyzes The Alpha-1,6-Glucosidic Linkages In Glycogen Which Has First Been Partially Depolymerized By Phosphorylase. Shows Only Very Little Activity With Native Glycogen. [C]

COG id: COG1523

COG function: function code G; Type II secretory pathway, pullulanase PulA and related glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Escherichia coli, GI2367229, Length=664, Percent_Identity=45.933734939759, Blast_Score=564, Evalue=1e-162,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004193
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR011837
- InterPro:   IPR013783
- InterPro:   IPR014756 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02922 CBM_48 [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 80296; Mature: 80296

Theoretical pI: Translated: 5.96; Mature: 5.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRAEVLPGKPYPLGATFDGQGVNFAVFSEHAKKVEVCLFDPEDPAKETRRFPLLETTHQ
CCCCCCCCCCCCCCCCEECCCCCEEEEECCCCCEEEEEEECCCCCHHHHHCCCCHHHHHH
VWHGYVPGLAAGALYGLRVHGPYEPKKGLRFNPHKLLVDPYARAIHGQVDYQAPIYAYTP
HHHCCCCHHHHHHEEEEEEECCCCCCCCCCCCCCEEEECHHHHHHCCCCCCCCCEEEECC
GTKEDDLAFDTRDDAAAVPKGVVMGADTFDWEGDTPPGVPWHDTLIYEVHVKGFTQLHPR
CCCCCCCEECCCCCHHHCCCCEEECCCCCCCCCCCCCCCCCCCEEEEEEEECCHHHCCCC
VPEALRGTYAGLAHPASIEHLKKVGVTAVELLPIQHIVDEPFLIQREKVNYWGYNTLGFF
CCHHHHHHHHCCCCCHHHHHHHHCCCHHEEHHHHHHHHCCCCEEEEECCCCCCCCCCEEE
APDARYSGSGSLGGQVDEFKRMVKQLHRAGIEVLLDVVYNHTCEGNQLGPTLSFKGVDNA
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCC
AYYRLTEKDPRYYLDVTGCGNSWNATHPYALKLVADSLRYWVEEMHVDGFRFDLATTLGR
EEEEEECCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEHHHHHCC
DRHGYDTRAAFFQIIHQDPVLSRVKLISEPWDVGDFGYQVGNFPVLWSEWNGKYRDTIRR
CCCCCCHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCEECCCEEEEEECCCCHHHHHHHH
YWKGDDRQAAEIGYRLTGSSDLFSLSGRKPAASVNFVTAHDGFTLHDLVTYNDKHNEANG
HHCCCCCCHHHCCEEEECCCCEEEECCCCCCCEEEEEEECCCEEEEEEEEECCCCCCCCC
EENRDGANDNHSWNCGVEGETTDAEINALREQQKRNFLATLFLSQGVPMLVAGDEMGRTQ
CCCCCCCCCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCC
KGNNNAYCQDNELSWVDWELNDTQRALLDFTCALTKLRREQPVLHKRRFFRGAHMWDSEL
CCCCCCEEECCCCEEEEEECCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCHHHH
KDLAWFRPDGQEMRKDDWEKPYVRSLGFLLGGDAITAPDDEGNRIVGDTILVLMNAHHEP
HHHEEECCCHHHHHHCCCCCHHHHHHHHHCCCCEEECCCCCCCEEECCEEEEEEECCCCC
ITFRLPAVEWGADWELVVDTALAGESLRTHTPAGGTVQVVGRSLAVLRRPATE
EEEEECCEECCCCCEEEEEEHHCCCCCCCCCCCCCEEEHHHHHHHHHCCCCCC
>Mature Secondary Structure
MRRAEVLPGKPYPLGATFDGQGVNFAVFSEHAKKVEVCLFDPEDPAKETRRFPLLETTHQ
CCCCCCCCCCCCCCCCEECCCCCEEEEECCCCCEEEEEEECCCCCHHHHHCCCCHHHHHH
VWHGYVPGLAAGALYGLRVHGPYEPKKGLRFNPHKLLVDPYARAIHGQVDYQAPIYAYTP
HHHCCCCHHHHHHEEEEEEECCCCCCCCCCCCCCEEEECHHHHHHCCCCCCCCCEEEECC
GTKEDDLAFDTRDDAAAVPKGVVMGADTFDWEGDTPPGVPWHDTLIYEVHVKGFTQLHPR
CCCCCCCEECCCCCHHHCCCCEEECCCCCCCCCCCCCCCCCCCEEEEEEEECCHHHCCCC
VPEALRGTYAGLAHPASIEHLKKVGVTAVELLPIQHIVDEPFLIQREKVNYWGYNTLGFF
CCHHHHHHHHCCCCCHHHHHHHHCCCHHEEHHHHHHHHCCCCEEEEECCCCCCCCCCEEE
APDARYSGSGSLGGQVDEFKRMVKQLHRAGIEVLLDVVYNHTCEGNQLGPTLSFKGVDNA
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCC
AYYRLTEKDPRYYLDVTGCGNSWNATHPYALKLVADSLRYWVEEMHVDGFRFDLATTLGR
EEEEEECCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEHHHHHCC
DRHGYDTRAAFFQIIHQDPVLSRVKLISEPWDVGDFGYQVGNFPVLWSEWNGKYRDTIRR
CCCCCCHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCEECCCEEEEEECCCCHHHHHHHH
YWKGDDRQAAEIGYRLTGSSDLFSLSGRKPAASVNFVTAHDGFTLHDLVTYNDKHNEANG
HHCCCCCCHHHCCEEEECCCCEEEECCCCCCCEEEEEEECCCEEEEEEEEECCCCCCCCC
EENRDGANDNHSWNCGVEGETTDAEINALREQQKRNFLATLFLSQGVPMLVAGDEMGRTQ
CCCCCCCCCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCC
KGNNNAYCQDNELSWVDWELNDTQRALLDFTCALTKLRREQPVLHKRRFFRGAHMWDSEL
CCCCCCEEECCCCEEEEEECCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCHHHH
KDLAWFRPDGQEMRKDDWEKPYVRSLGFLLGGDAITAPDDEGNRIVGDTILVLMNAHHEP
HHHEEECCCHHHHHHCCCCCHHHHHHHHHCCCCEEECCCCCCCEEECCEEEEEEECCCCC
ITFRLPAVEWGADWELVVDTALAGESLRTHTPAGGTVQVVGRSLAVLRRPATE
EEEEECCEECCCCCEEEEEEHHCCCCCCCCCCCCCEEEHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Glycosylases; Glycosidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]