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The map label for this gene is fumB [H]

Identifier: 108757029

GI number: 108757029

Start: 3704909

End: 3706618

Strand: Reverse

Name: fumB [H]

Synonym: MXAN_3162

Alternate gene names: 108757029

Gene position: 3706618-3704909 (Counterclockwise)

Preceding gene: 108761242

Following gene: 108758585

Centisome position: 40.55

GC content: 66.9

Gene sequence:

>1710_bases
ATGCTGGGAGGTTTTCTCGCGCCGCGAGTTCCAGCTACCCACGAGGGCCGCATCAGCGCTACGCCTACGGGTATGACCGA
CTTCCAGTTCCAGGACATGCTGCCGCTGGGCAAGGACGAGACGCCGTACCGGCTGCTCTCCAAGGACCACGTCTCCACCT
TCGAAGCCGGCGGCCGCACCTTCCTCCAGGTCGCGCCCGAGGCGCTCACCCTGCTCACCCGCGAGGCCATGCGCGACATC
GCGCACCTGCTGCGCCCCGGGCACCTGAAGCAGCTCTCCCACATCCTCGAGGACCCCGAGGCGTCATCCAACGACCGCTT
CGTGGCGCTGGAGCTGCTGAAGAACGCCAACATCGCCGCCGGCGGCGTGCTGCCCTCCTGCCAGGACACGGGCACCGCCA
TCGTCATGGGCAAGAAGGGCCAGTACGTCATCACCGGTGGCAATGACGAGGAGGCCATCTCCCGCGGCGTGTTCGAGACG
TACCAGACGTCGAACCTGCGCTACTCGCAGATGGCGCCGCTGGACATGTACAAGGAGGTCAACACCGGCAACAACCTGCC
CGCGCAGATCGAGCTCTACGCGACGGACGGTGACGCCTACAAGTTCCTCTTCATGGCGAAGGGCGGCGGCTCCGCCAACA
AGAGCTACCTGTTCCAGGAGACGAAGGCGCTGCTCAACCCGCAGAGCCTGCTGAACTTCGTGGACGCGAAGATCCGCTCG
CTGGGCACCGCGGCCTGTCCGCCGTACCACCTGGCCATCGTCATTGGCGGCACCTCCGCCGAATACGCGCTGAAGACGGC
CAAGTACGCCTCCGCCCGCTACCTGGATTCGCTCCCCACCGAGGGCAACAAGCTGGGTCGCGGCTTCCGCGACGTGGCGC
TGGAGCAGGAGATCCTCAAGCTCACGCAGCGCACCGGCATTGGCGCGCAGTTCGGCGGCAAGTACTTCTGCCACGACGTG
CGCGTCATCCGCCTGCCCCGCCACGGCGCGTCCTGCCCGGTGGCCATCGCGGTGTCGTGCTCGGCGGACCGGCAGATCCT
GGGGAAAATCACCCAGGACGGCATCTTCCTGGAGCAGTTGGAGGCCGACCCGGCGAAGTACCTGCCGGAGACGACGGACG
GGGAGCTGGGCGGCGAGGTGGTGAAGATCGACCTCAACCGCCCCATGAGCGAGATTCGCGCGGAGCTGTCGCGCTACCCC
ATCAAGACGCGCCTGTCGCTCAGCGGCCCCCTGGTGGTGGCGCGCGACATCGCCCACGCGAAGATCAAGGAGGTGCTGGA
CGCCGGCAACGGCATGCCCCAGTACCTCAAGGACTACATGGTCTACTACGCGGGCCCGGCGAAGACGCCGGAAGGCTATG
CCTCGGGCTCCTTCGGCCCGACGACGGCCGGCCGCATGGACGCGTACGTGGACCAGTTCCAGGCCGCGGGCGGCAGCTTC
GTGATGCTGGCCAAGGGCAACCGCTCCCCCGCCGTCACCGAGGCCTGCAAGAAGCACGGTGGCTTCTATCTGGGCTCCAT
CGGCGGCCCGGCGGCGCGGCTGGCCAAGGACTGCATCACCAAGGTGGAGGTGCTCGAGTACGCCGAGCTGGGCATGGAGG
CCGTGTGGAAGATTGAAGTGGTGGACTTCCCGGCCTTCATCGTCGTGGACGACAAGGGCAACGACTTCTTCGCCAACATC
AACAAGCCGTCGGCGGCGAAGAAGGCCTGA

Upstream 100 bases:

>100_bases
TTGTCCGCCACCCAGGCTGACGACCAGCCAACCCTCGGCCACCTTCCCTCCCCTGTCCATGTATCAGGCTGTATCTGGAG
TGAAGGGACTTGTAGCTCCC

Downstream 100 bases:

>100_bases
ACGGGAGCCACCCGCCCCTGACGCTCAGGGACGAGGCGGCTCCCCGACCTTGGGAGCCGCCGTGGCGGAGGCGCGGCGCT
TCGGGATGATGGAGCGCCGT

Product: fumarate hydratase, class I

Products: NA

Alternate protein names: Fumarase [H]

Number of amino acids: Translated: 569; Mature: 569

Protein sequence:

>569_residues
MLGGFLAPRVPATHEGRISATPTGMTDFQFQDMLPLGKDETPYRLLSKDHVSTFEAGGRTFLQVAPEALTLLTREAMRDI
AHLLRPGHLKQLSHILEDPEASSNDRFVALELLKNANIAAGGVLPSCQDTGTAIVMGKKGQYVITGGNDEEAISRGVFET
YQTSNLRYSQMAPLDMYKEVNTGNNLPAQIELYATDGDAYKFLFMAKGGGSANKSYLFQETKALLNPQSLLNFVDAKIRS
LGTAACPPYHLAIVIGGTSAEYALKTAKYASARYLDSLPTEGNKLGRGFRDVALEQEILKLTQRTGIGAQFGGKYFCHDV
RVIRLPRHGASCPVAIAVSCSADRQILGKITQDGIFLEQLEADPAKYLPETTDGELGGEVVKIDLNRPMSEIRAELSRYP
IKTRLSLSGPLVVARDIAHAKIKEVLDAGNGMPQYLKDYMVYYAGPAKTPEGYASGSFGPTTAGRMDAYVDQFQAAGGSF
VMLAKGNRSPAVTEACKKHGGFYLGSIGGPAARLAKDCITKVEVLEYAELGMEAVWKIEVVDFPAFIVVDDKGNDFFANI
NKPSAAKKA

Sequences:

>Translated_569_residues
MLGGFLAPRVPATHEGRISATPTGMTDFQFQDMLPLGKDETPYRLLSKDHVSTFEAGGRTFLQVAPEALTLLTREAMRDI
AHLLRPGHLKQLSHILEDPEASSNDRFVALELLKNANIAAGGVLPSCQDTGTAIVMGKKGQYVITGGNDEEAISRGVFET
YQTSNLRYSQMAPLDMYKEVNTGNNLPAQIELYATDGDAYKFLFMAKGGGSANKSYLFQETKALLNPQSLLNFVDAKIRS
LGTAACPPYHLAIVIGGTSAEYALKTAKYASARYLDSLPTEGNKLGRGFRDVALEQEILKLTQRTGIGAQFGGKYFCHDV
RVIRLPRHGASCPVAIAVSCSADRQILGKITQDGIFLEQLEADPAKYLPETTDGELGGEVVKIDLNRPMSEIRAELSRYP
IKTRLSLSGPLVVARDIAHAKIKEVLDAGNGMPQYLKDYMVYYAGPAKTPEGYASGSFGPTTAGRMDAYVDQFQAAGGSF
VMLAKGNRSPAVTEACKKHGGFYLGSIGGPAARLAKDCITKVEVLEYAELGMEAVWKIEVVDFPAFIVVDDKGNDFFANI
NKPSAAKKA
>Mature_569_residues
MLGGFLAPRVPATHEGRISATPTGMTDFQFQDMLPLGKDETPYRLLSKDHVSTFEAGGRTFLQVAPEALTLLTREAMRDI
AHLLRPGHLKQLSHILEDPEASSNDRFVALELLKNANIAAGGVLPSCQDTGTAIVMGKKGQYVITGGNDEEAISRGVFET
YQTSNLRYSQMAPLDMYKEVNTGNNLPAQIELYATDGDAYKFLFMAKGGGSANKSYLFQETKALLNPQSLLNFVDAKIRS
LGTAACPPYHLAIVIGGTSAEYALKTAKYASARYLDSLPTEGNKLGRGFRDVALEQEILKLTQRTGIGAQFGGKYFCHDV
RVIRLPRHGASCPVAIAVSCSADRQILGKITQDGIFLEQLEADPAKYLPETTDGELGGEVVKIDLNRPMSEIRAELSRYP
IKTRLSLSGPLVVARDIAHAKIKEVLDAGNGMPQYLKDYMVYYAGPAKTPEGYASGSFGPTTAGRMDAYVDQFQAAGGSF
VMLAKGNRSPAVTEACKKHGGFYLGSIGGPAARLAKDCITKVEVLEYAELGMEAVWKIEVVDFPAFIVVDDKGNDFFANI
NKPSAAKKA

Specific function: It functions as an aerobic enzyme in the citric acid cycle. It accounts for about 80% of the fumarase activity when the bacteria grows aerobically [H]

COG id: COG1951

COG function: function code C; Tartrate dehydratase alpha subunit/Fumarate hydratase class I, N-terminal domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I fumarase family [H]

Homologues:

Organism=Escherichia coli, GI1787897, Length=534, Percent_Identity=67.6029962546816, Blast_Score=763, Evalue=0.0,
Organism=Escherichia coli, GI1790564, Length=539, Percent_Identity=66.2337662337662, Blast_Score=748, Evalue=0.0,
Organism=Escherichia coli, GI1789443, Length=168, Percent_Identity=29.1666666666667, Blast_Score=78, Evalue=2e-15,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004646
- InterPro:   IPR004647
- InterPro:   IPR011167
- InterPro:   IPR020557 [H]

Pfam domain/function: PF05681 Fumerase; PF05683 Fumerase_C [H]

EC number: =4.2.1.2 [H]

Molecular weight: Translated: 61547; Mature: 61547

Theoretical pI: Translated: 7.04; Mature: 7.04

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLGGFLAPRVPATHEGRISATPTGMTDFQFQDMLPLGKDETPYRLLSKDHVSTFEAGGRT
CCCCCCCCCCCCCCCCEEEECCCCCCCCCHHHCCCCCCCCCCHHHHCCCCHHHHHCCCCE
FLQVAPEALTLLTREAMRDIAHLLRPGHLKQLSHILEDPEASSNDRFVALELLKNANIAA
EEEHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCEEEEEEHHCCCEEE
GGVLPSCQDTGTAIVMGKKGQYVITGGNDEEAISRGVFETYQTSNLRYSQMAPLDMYKEV
CCCCCCCCCCCCEEEEECCCCEEEECCCCHHHHHHHHHHHHHCCCCCHHHCCCHHHHHHC
NTGNNLPAQIELYATDGDAYKFLFMAKGGGSANKSYLFQETKALLNPQSLLNFVDAKIRS
CCCCCCCEEEEEEEECCCEEEEEEEECCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHH
LGTAACPPYHLAIVIGGTSAEYALKTAKYASARYLDSLPTEGNKLGRGFRDVALEQEILK
CCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCHHHCCCHHHHHHHHHHHH
LTQRTGIGAQFGGKYFCHDVRVIRLPRHGASCPVAIAVSCSADRQILGKITQDGIFLEQL
HHHHCCCCHHCCCEEEEECEEEEEECCCCCCCCEEEEEECCCCHHHHHHHHCCCEEHHHH
EADPAKYLPETTDGELGGEVVKIDLNRPMSEIRAELSRYPIKTRLSLSGPLVVARDIAHA
CCCHHHHCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHCCCEEEEECCCCEEEEHHHHHH
KIKEVLDAGNGMPQYLKDYMVYYAGPAKTPEGYASGSFGPTTAGRMDAYVDQFQAAGGSF
HHHHHHHCCCCCHHHHHHHEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCE
VMLAKGNRSPAVTEACKKHGGFYLGSIGGPAARLAKDCITKVEVLEYAELGMEAVWKIEV
EEEECCCCCCHHHHHHHHHCCEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEE
VDFPAFIVVDDKGNDFFANINKPSAAKKA
EECCEEEEEECCCCCEEEECCCCCCCCCC
>Mature Secondary Structure
MLGGFLAPRVPATHEGRISATPTGMTDFQFQDMLPLGKDETPYRLLSKDHVSTFEAGGRT
CCCCCCCCCCCCCCCCEEEECCCCCCCCCHHHCCCCCCCCCCHHHHCCCCHHHHHCCCCE
FLQVAPEALTLLTREAMRDIAHLLRPGHLKQLSHILEDPEASSNDRFVALELLKNANIAA
EEEHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCEEEEEEHHCCCEEE
GGVLPSCQDTGTAIVMGKKGQYVITGGNDEEAISRGVFETYQTSNLRYSQMAPLDMYKEV
CCCCCCCCCCCCEEEEECCCCEEEECCCCHHHHHHHHHHHHHCCCCCHHHCCCHHHHHHC
NTGNNLPAQIELYATDGDAYKFLFMAKGGGSANKSYLFQETKALLNPQSLLNFVDAKIRS
CCCCCCCEEEEEEEECCCEEEEEEEECCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHH
LGTAACPPYHLAIVIGGTSAEYALKTAKYASARYLDSLPTEGNKLGRGFRDVALEQEILK
CCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCHHHCCCHHHHHHHHHHHH
LTQRTGIGAQFGGKYFCHDVRVIRLPRHGASCPVAIAVSCSADRQILGKITQDGIFLEQL
HHHHCCCCHHCCCEEEEECEEEEEECCCCCCCCEEEEEECCCCHHHHHHHHCCCEEHHHH
EADPAKYLPETTDGELGGEVVKIDLNRPMSEIRAELSRYPIKTRLSLSGPLVVARDIAHA
CCCHHHHCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHCCCEEEEECCCCEEEEHHHHHH
KIKEVLDAGNGMPQYLKDYMVYYAGPAKTPEGYASGSFGPTTAGRMDAYVDQFQAAGGSF
HHHHHHHCCCCCHHHHHHHEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCE
VMLAKGNRSPAVTEACKKHGGFYLGSIGGPAARLAKDCITKVEVLEYAELGMEAVWKIEV
EEEECCCCCCHHHHHHHHHCCEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEE
VDFPAFIVVDDKGNDFFANINKPSAAKKA
EECCEEEEEECCCCCEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11677609; 1879695 [H]