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The map label for this gene is ddlA [C]

Identifier: 108757010

GI number: 108757010

Start: 5041602

End: 5043791

Strand: Reverse

Name: ddlA [C]

Synonym: MXAN_4097

Alternate gene names: 108757010

Gene position: 5043791-5041602 (Counterclockwise)

Preceding gene: 108758059

Following gene: 108758847

Centisome position: 55.19

GC content: 69.0

Gene sequence:

>2190_bases
ATGCGCATCGCGCTGACCCACAACCTCAGGCTGTCTGATTTGGAAGAAGAGGCGGAGTTCGACACGCAGGAGACGGTCAA
CGCGCTCGCGGCGGCCATCGAGCGCATCGGCCACCGGCTGGAGCGCTTCGAGGTGAGCGGCCCGGCCTCGCGCACCGTGG
CGCGGCTGGAGGCCTACAGCCCGGACCTCATCTTCAACACCGCCGAGGGCCGCCGCGGCCGCTTCCGCGAGGCCTTCTAT
CCGGCCCTCTTCGACGAGCTGGGCTTCCCGTACACGGGCTCGGACGCGTACGCGCTGGCGGTGACGCTCGACAAGCAGCT
CACCAAGCTGGTGCTGTCCAAGCAGGGCATCCGCACGCCCGGCTGGCAGTACGTGGAGAAGCTCAGCGAGCTGTCCGCGG
AGAACCTGCGCTTCCCTGTCATCGTGAAGCCCAACTTCGAGGGCTCGTCCAAGGGCATCAGCCAGGACTCCATCGCGGAG
ACGCTGGACGAGGTTCGCGCCAAGGTGGCCGCCGCGCTGGAGAAGTACCCGTCCGGCGTGCTGGTGGAGGAGTACATCTC
CGGCCGCGACCTCGCCGTGCCGTTCCTGGAGTCGGTGGACAATGACTACGACGGCGTGCTCTCCCCGGTGGAGTACGTCA
TCGACCCGGCCGTCACGCAGGGCCGCAAGTACGCCATCTACGACTATGCGCTGAAGACGTCGCGGGAGGGCACGGTCAGC
GTGCGCGCCCCTGCGCAGATTCCGCCTCGCACGGCGGAGGACGTCCGGCGGATGGCGCAAAAGGTGTACCGGGCGCTCGA
CTGCCGCGACCTGGGCCGCATCGACGTGCGCCTCAGTGACGCCGGCGTGCCGTACTTCCTGGAGATCAACGCGCTCCCCA
GCCTGGAGCCGGGCGCGGGCATCTACGCGGCGGCGGAGCTGGACGGCCTGCACCTGGATGGGGTCATCAACTCCATCATC
ACCAGCGCGGCGAAGCGGTACAAAATCAAGGACTCGTCGCGTCGCCAGGGCAAGCCAGCGCGCAAGAGCGGTCCGCTGCG
CGTGGGCTTCAGCTTCAACGTGAAGCGCGTGAAGCCCACGGCCACGGCGGAGGCGGTGGAGGACAGCGAGGCCGAGTACG
ACTCGCCGAACACGCTCCAGGCCATCCGCGAGGCGATTGCGTCGTGGGGCCACGAGGTCATCGACCTGGAGGCCACGGCC
GAGCTTCCCTCGGTGCTGTCGAGCACGCCGCTGGACGTGGTGTTCAACATCGCGGAGGGCTTCAAGGGCCGCAACCGAGA
GAGCCAGGTCCCGGCGCTGCTGGAGCTGCTGGACATCCCGTACACGGGCTCGGACCCGGCCACGCTGTCCATCGCCCTGG
ACAAGGGCCTGGCGAAGAAGATTGTCCGGCAGGCTGGCATCCTCACGCCCAACTTCCAGCTCATGGCGACGGGCAAGGAG
CGCCTCAACAAGGAGTTCACCAGCTTCCCGCTCATCGTGAAGCCGGTGGCGGAGGGCAGCTCCAAGGGCGTCGTCACCAA
GAGCGTCTGCCACAGCGAGGCGGAGCTGCGCGAGGTGGTGCGCGAGATTGCCGGCAAGTACCAGCAGCCCGCGCTCATCG
AGGAGTACATCCGCGGCCGTGAGTTCACCGTGGGCCTGCTGGGCGAGCGGCGTCCCCGCGTCCTGCCGCCCATGGAGATC
GTCTTCCTGGACCGGGAGGAGAAGAACCCCGTCTACAGCTTCCAGCACAAGCTCGATTGGACGGACCGCATCCGCTACGA
CGCGCCGGCGAAGCTGGAGCCCGCGCTGCTGGAGCGGCTGCGCACGGCGGCGCGCAACTCGTTCATGGCGCTGGGATGCC
GCGACGTCGCGCGCATCGACTTCCGCATGGATGACAAGGGGCGCATCTACTTCATCGAGTGCAACCCGCTGCCGGGCCTG
ACGCCGGGCTGGAGCGACCTGGTGCTCATCGCCCAGGGCGCCGGCATGGACTACCGGGGGCTCATCGGCGAAATCATGGC
CCCCGCCATCCCCCGCTACAAGGAGCGCGAGGCGCGCCGCGCCGCCAGCGAGCACCCCGCTTCGGTGTTGCGCAAGAGCG
TGCCCCTGGAGGAGGGGACTCCCGCCGCCGCGCAGCCCGTGGCCGCGACGGCCCCCGCACCGGCATCCCCAGCGCCGGAG
CCGTCTCCGCGCATGGACGCGAAGGCCTGA

Upstream 100 bases:

>100_bases
CTCGCACGCCGGTCGCGCATGAGCGCGCCTCCGGCAGGCTCATGTCCGCGGCGAAGGTGCGCCGTGGACGCTCGTCGTTC
AGGAAGCGAAGGGGGTACCC

Downstream 100 bases:

>100_bases
GTCCCACGCGACGCCCTGGCCGCCCCGTCCCATGCCTCCTCTTCGAGGGGGCGGGCGGGGCTGCCTGGAGTGGTTGACTG
TGGGGATCGGTCGGTTAGTG

Product: D-alanine-D-alanine ligase family protein

Products: ADP; phosphate; D-alanyl-D-alanine

Alternate protein names: NA

Number of amino acids: Translated: 729; Mature: 729

Protein sequence:

>729_residues
MRIALTHNLRLSDLEEEAEFDTQETVNALAAAIERIGHRLERFEVSGPASRTVARLEAYSPDLIFNTAEGRRGRFREAFY
PALFDELGFPYTGSDAYALAVTLDKQLTKLVLSKQGIRTPGWQYVEKLSELSAENLRFPVIVKPNFEGSSKGISQDSIAE
TLDEVRAKVAAALEKYPSGVLVEEYISGRDLAVPFLESVDNDYDGVLSPVEYVIDPAVTQGRKYAIYDYALKTSREGTVS
VRAPAQIPPRTAEDVRRMAQKVYRALDCRDLGRIDVRLSDAGVPYFLEINALPSLEPGAGIYAAAELDGLHLDGVINSII
TSAAKRYKIKDSSRRQGKPARKSGPLRVGFSFNVKRVKPTATAEAVEDSEAEYDSPNTLQAIREAIASWGHEVIDLEATA
ELPSVLSSTPLDVVFNIAEGFKGRNRESQVPALLELLDIPYTGSDPATLSIALDKGLAKKIVRQAGILTPNFQLMATGKE
RLNKEFTSFPLIVKPVAEGSSKGVVTKSVCHSEAELREVVREIAGKYQQPALIEEYIRGREFTVGLLGERRPRVLPPMEI
VFLDREEKNPVYSFQHKLDWTDRIRYDAPAKLEPALLERLRTAARNSFMALGCRDVARIDFRMDDKGRIYFIECNPLPGL
TPGWSDLVLIAQGAGMDYRGLIGEIMAPAIPRYKEREARRAASEHPASVLRKSVPLEEGTPAAAQPVAATAPAPASPAPE
PSPRMDAKA

Sequences:

>Translated_729_residues
MRIALTHNLRLSDLEEEAEFDTQETVNALAAAIERIGHRLERFEVSGPASRTVARLEAYSPDLIFNTAEGRRGRFREAFY
PALFDELGFPYTGSDAYALAVTLDKQLTKLVLSKQGIRTPGWQYVEKLSELSAENLRFPVIVKPNFEGSSKGISQDSIAE
TLDEVRAKVAAALEKYPSGVLVEEYISGRDLAVPFLESVDNDYDGVLSPVEYVIDPAVTQGRKYAIYDYALKTSREGTVS
VRAPAQIPPRTAEDVRRMAQKVYRALDCRDLGRIDVRLSDAGVPYFLEINALPSLEPGAGIYAAAELDGLHLDGVINSII
TSAAKRYKIKDSSRRQGKPARKSGPLRVGFSFNVKRVKPTATAEAVEDSEAEYDSPNTLQAIREAIASWGHEVIDLEATA
ELPSVLSSTPLDVVFNIAEGFKGRNRESQVPALLELLDIPYTGSDPATLSIALDKGLAKKIVRQAGILTPNFQLMATGKE
RLNKEFTSFPLIVKPVAEGSSKGVVTKSVCHSEAELREVVREIAGKYQQPALIEEYIRGREFTVGLLGERRPRVLPPMEI
VFLDREEKNPVYSFQHKLDWTDRIRYDAPAKLEPALLERLRTAARNSFMALGCRDVARIDFRMDDKGRIYFIECNPLPGL
TPGWSDLVLIAQGAGMDYRGLIGEIMAPAIPRYKEREARRAASEHPASVLRKSVPLEEGTPAAAQPVAATAPAPASPAPE
PSPRMDAKA
>Mature_729_residues
MRIALTHNLRLSDLEEEAEFDTQETVNALAAAIERIGHRLERFEVSGPASRTVARLEAYSPDLIFNTAEGRRGRFREAFY
PALFDELGFPYTGSDAYALAVTLDKQLTKLVLSKQGIRTPGWQYVEKLSELSAENLRFPVIVKPNFEGSSKGISQDSIAE
TLDEVRAKVAAALEKYPSGVLVEEYISGRDLAVPFLESVDNDYDGVLSPVEYVIDPAVTQGRKYAIYDYALKTSREGTVS
VRAPAQIPPRTAEDVRRMAQKVYRALDCRDLGRIDVRLSDAGVPYFLEINALPSLEPGAGIYAAAELDGLHLDGVINSII
TSAAKRYKIKDSSRRQGKPARKSGPLRVGFSFNVKRVKPTATAEAVEDSEAEYDSPNTLQAIREAIASWGHEVIDLEATA
ELPSVLSSTPLDVVFNIAEGFKGRNRESQVPALLELLDIPYTGSDPATLSIALDKGLAKKIVRQAGILTPNFQLMATGKE
RLNKEFTSFPLIVKPVAEGSSKGVVTKSVCHSEAELREVVREIAGKYQQPALIEEYIRGREFTVGLLGERRPRVLPPMEI
VFLDREEKNPVYSFQHKLDWTDRIRYDAPAKLEPALLERLRTAARNSFMALGCRDVARIDFRMDDKGRIYFIECNPLPGL
TPGWSDLVLIAQGAGMDYRGLIGEIMAPAIPRYKEREARRAASEHPASVLRKSVPLEEGTPAAAQPVAATAPAPASPAPE
PSPRMDAKA

Specific function: Could be involved in the biosynthesis of a cell wall component [H]

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1786579, Length=296, Percent_Identity=28.3783783783784, Blast_Score=112, Evalue=1e-25,
Organism=Escherichia coli, GI1786280, Length=269, Percent_Identity=30.8550185873606, Blast_Score=105, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR011095
- InterPro:   IPR016185 [H]

Pfam domain/function: PF07478 Dala_Dala_lig_C [H]

EC number: 6.3.2.4

Molecular weight: Translated: 80307; Mature: 80307

Theoretical pI: Translated: 5.73; Mature: 5.73

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIALTHNLRLSDLEEEAEFDTQETVNALAAAIERIGHRLERFEVSGPASRTVARLEAYS
CEEEEECCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCC
PDLIFNTAEGRRGRFREAFYPALFDELGFPYTGSDAYALAVTLDKQLTKLVLSKQGIRTP
CCEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCCEEEEEEHHHHHHHHHHHHCCCCCC
GWQYVEKLSELSAENLRFPVIVKPNFEGSSKGISQDSIAETLDEVRAKVAAALEKYPSGV
CHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
LVEEYISGRDLAVPFLESVDNDYDGVLSPVEYVIDPAVTQGRKYAIYDYALKTSREGTVS
CHHHHHCCCCCCCHHHHHCCCCCCHHHHHHHHHHCHHHHCCCEEEEEEEHHHCCCCCEEE
VRAPAQIPPRTAEDVRRMAQKVYRALDCRDLGRIDVRLSDAGVPYFLEINALPSLEPGAG
EECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCEEEEECCCCCCCCCCC
IYAAAELDGLHLDGVINSIITSAAKRYKIKDSSRRQGKPARKSGPLRVGFSFNVKRVKPT
EEEEECCCCEEHHHHHHHHHHHHHHHEECCCCHHHCCCCCCCCCCEEEEEEECEEEECCC
ATAEAVEDSEAEYDSPNTLQAIREAIASWGHEVIDLEATAELPSVLSSTPLDVVFNIAEG
HHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHCCCCHHHHHHHHHC
FKGRNRESQVPALLELLDIPYTGSDPATLSIALDKGLAKKIVRQAGILTPNFQLMATGKE
CCCCCCCHHHHHHHHHHCCCCCCCCCCEEEEEEHHHHHHHHHHHHCCCCCCCEEEECCHH
RLNKEFTSFPLIVKPVAEGSSKGVVTKSVCHSEAELREVVREIAGKYQQPALIEEYIRGR
HHHHHHHCCCEEEEECCCCCCCCCEEHHHHCCHHHHHHHHHHHHCCCCCCHHHHHHHCCC
EFTVGLLGERRPRVLPPMEIVFLDREEKNPVYSFQHKLDWTDRIRYDAPAKLEPALLERL
CEEEEECCCCCCCCCCCEEEEEECCCCCCCCCHHHHCCCHHHCCCCCCCCCCCHHHHHHH
RTAARNSFMALGCRDVARIDFRMDDKGRIYFIECNPLPGLTPGWSDLVLIAQGAGMDYRG
HHHHHCCEEEECCCHHHEEEEEECCCCCEEEEECCCCCCCCCCCCCEEEEEECCCCCHHH
LIGEIMAPAIPRYKEREARRAASEHPASVLRKSVPLEEGTPAAAQPVAATAPAPASPAPE
HHHHHHHCCCCCHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCC
PSPRMDAKA
CCCCCCCCC
>Mature Secondary Structure
MRIALTHNLRLSDLEEEAEFDTQETVNALAAAIERIGHRLERFEVSGPASRTVARLEAYS
CEEEEECCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCC
PDLIFNTAEGRRGRFREAFYPALFDELGFPYTGSDAYALAVTLDKQLTKLVLSKQGIRTP
CCEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCCEEEEEEHHHHHHHHHHHHCCCCCC
GWQYVEKLSELSAENLRFPVIVKPNFEGSSKGISQDSIAETLDEVRAKVAAALEKYPSGV
CHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
LVEEYISGRDLAVPFLESVDNDYDGVLSPVEYVIDPAVTQGRKYAIYDYALKTSREGTVS
CHHHHHCCCCCCCHHHHHCCCCCCHHHHHHHHHHCHHHHCCCEEEEEEEHHHCCCCCEEE
VRAPAQIPPRTAEDVRRMAQKVYRALDCRDLGRIDVRLSDAGVPYFLEINALPSLEPGAG
EECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCEEEEECCCCCCCCCCC
IYAAAELDGLHLDGVINSIITSAAKRYKIKDSSRRQGKPARKSGPLRVGFSFNVKRVKPT
EEEEECCCCEEHHHHHHHHHHHHHHHEECCCCHHHCCCCCCCCCCEEEEEEECEEEECCC
ATAEAVEDSEAEYDSPNTLQAIREAIASWGHEVIDLEATAELPSVLSSTPLDVVFNIAEG
HHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHCCCCHHHHHHHHHC
FKGRNRESQVPALLELLDIPYTGSDPATLSIALDKGLAKKIVRQAGILTPNFQLMATGKE
CCCCCCCHHHHHHHHHHCCCCCCCCCCEEEEEEHHHHHHHHHHHHCCCCCCCEEEECCHH
RLNKEFTSFPLIVKPVAEGSSKGVVTKSVCHSEAELREVVREIAGKYQQPALIEEYIRGR
HHHHHHHCCCEEEEECCCCCCCCCEEHHHHCCHHHHHHHHHHHHCCCCCCHHHHHHHCCC
EFTVGLLGERRPRVLPPMEIVFLDREEKNPVYSFQHKLDWTDRIRYDAPAKLEPALLERL
CEEEEECCCCCCCCCCCEEEEEECCCCCCCCCHHHHCCCHHHCCCCCCCCCCCHHHHHHH
RTAARNSFMALGCRDVARIDFRMDDKGRIYFIECNPLPGLTPGWSDLVLIAQGAGMDYRG
HHHHHCCEEEECCCHHHEEEEEECCCCCEEEEECCCCCCCCCCCCCEEEEEECCCCCHHH
LIGEIMAPAIPRYKEREARRAASEHPASVLRKSVPLEEGTPAAAQPVAATAPAPASPAPE
HHHHHHHCCCCCHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCC
PSPRMDAKA
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; D-alanine

Specific reaction: ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine

General reaction: Carboxamide formation; Carboxylic acid amide formation [C]

Inhibitor: (3-Amino-2-oxoalkyl) phosphonic acids; alpha-Aminophosphonamidic acid; D-(1-Aminoethyl) phosphonic acid in presence of ATP; D-Cycloserine; Tabtoxinine [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]