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The map label for this gene is anfA [H]

Identifier: 108756928

GI number: 108756928

Start: 3875770

End: 3877470

Strand: Direct

Name: anfA [H]

Synonym: MXAN_3333

Alternate gene names: 108756928

Gene position: 3875770-3877470 (Clockwise)

Preceding gene: 108760839

Following gene: 108763576

Centisome position: 42.41

GC content: 73.96

Gene sequence:

>1701_bases
ATGCCCGCCCTCCTGCTGCTCACCGGTCCTTCCGCGGGGCGTCGTCACGACGTGCTCGCGGAGGCGACGATTGGCCGCAG
TCCCTCGTGTGAAATCCCGCTGGATGACCATCAGGTCTCCCGCAAGCACGCGCTGATTTCCGTTCTCGACGGGCAGGCGC
GCATCCGCGACTTGCGCTCGCGCAACGGGACGTTCGTCAACGGCGAGCGCCTCGTGGATGAGGTGGTGCTCAAGCCCGGT
GACCAGGTGCGCGTGGGCGTGACGACGGCCCTCTTCGAGCCGCCCCCGGTGACCCTGGTGGCCGATGGGCCGGAGAAGAT
GGGGCAGGTGCCCATCGAGGAAGTGCTGCCTCACGTGGGCGCGGCGGCGGCGATGTACTCGGCGGGCATCGCCCTGCTGG
GCGCGACGAGTGGCGCCCTGGTGCTGCGGCGCCTGGCGGATGAGGTCGCGCACGCACTCAATGCCGACCGCGCGGCCGCG
CTGCTGGGCAGCAGTGATGGCTTGCTCACCGCGGCGGTGGCGGGCGCGGAGGCGCTGACGATGCCGCGCGCGCTGGCGCA
GGCCGCGCTGGAGCGCAAGGAGTTGGTCCAGACGGATGACGTCCTCTGCGCGCCCCTGGTCGCCTCAGGCGGCATGCCCT
TCGGCGTGCTGTACGTGACGCGGGCGGAGCCGAAGTTCAGTGAGGGCGAGGGCCAGTTGCTCGCGGCGCTGGGACGGTTG
GGGGGCGAGGCCTACACCACCGTGCGCTCCCGCGTGGATGCGGAGCCGCCCGTGGCCGCGCTCTCCGGGACGTCCCGGCC
GCTGCGGGCCTTGCTGGAGCTGGCGCGCCGGGTGGCCGCCAGCGCCGCGCCGGTGGTGATTCATGGCGAGCCGGGCGTGG
GCAAGGCGCTGCTGGCTCGCTTCGTCCATGCGCGTTCGCCTCGGGCCCTGGGGCCTTTCATCGTGGTGGACTGCCGGGAG
CCGGCCGATGCCGTGGAGGAGGCGCTGTTCGGCAGGGCCAGCGCGCCCGGGCAGCCGCCTGTCGTATCCGCGCTGCTGCG
CGCGGATGGGGGCTCGCTGGTGCTGCTCCACGTGGAGTCGCTCGCGCGGCCTGCTTCGGAGCGGCTGGCGAGGGCCCTGG
CGCGCCGTTCAGCGCCCGCGCGGCAAGGCGGCGAGGAGCCCGTGGACGTTCGGGTGCTGGCCACCGCGCCCGCGTCGGTG
TCGTTGCTGGCGGCGCGCGGCGACGTGGAGGCCTCGTTGGCTCGGGCGCTGTCGGGCTTCGAGTTGGACGCCCCGCCCAT
CCGCGAGCGGCGCGCGGACGTGCTGCCGCTGCTGGAGCAGTTCGCGGCGCGCGCGGCCCGCCGGATTCGCAAGGAGCCAC
CGACGCTCAGTCCCGAGGCCCGGCGGCTGCTGACGGAGTACGGGTGGCCGCAGAACCTGCGGGAGCTGGAGCTGGTGGGG
GAGCGCCTGGGCCTGCTGTACGCGGCGAGCCGAGTGGGCGCGCTGCAACTGCCCCCGGAGTTCCAGGAGGGCGGCGACGA
GGGCGCGAAGACGTTGCAGGGACGCGTGGCCCGGCTGGAGCGCGATGCCATCGCCGAGGCCCTGCGCGAGGCGTCGGGAA
AGAAGGTCCGCGCCGCGGCGCTGCTCGGCATCAGCCGGCCCACGCTGGACAAGAAGATTGAGGAGTACGGGCTCGCGGTG
GAGCGGGGGCGCCGGGAGTGA

Upstream 100 bases:

>100_bases
GAGCGGGAACTCCGTGAACTCCTGAACGCCGAACGGCCGATGTAGCAAGCGGCGCCGGGGGCCTTGCCCAGCCATCGCGG
GGCGCTAGGCTCCGCGCCCC

Downstream 100 bases:

>100_bases
GTCAGGCCCCGGCGCCTACGACTTCCGCCGGCTGAGCAGCACGCCGGAGAGGACGAGCCCCGCTCCGAGCACCTGCATCC
AGGTGGGCTGCTCTCCACGC

Product: sigma-54 dependent trancsriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 566; Mature: 565

Protein sequence:

>566_residues
MPALLLLTGPSAGRRHDVLAEATIGRSPSCEIPLDDHQVSRKHALISVLDGQARIRDLRSRNGTFVNGERLVDEVVLKPG
DQVRVGVTTALFEPPPVTLVADGPEKMGQVPIEEVLPHVGAAAAMYSAGIALLGATSGALVLRRLADEVAHALNADRAAA
LLGSSDGLLTAAVAGAEALTMPRALAQAALERKELVQTDDVLCAPLVASGGMPFGVLYVTRAEPKFSEGEGQLLAALGRL
GGEAYTTVRSRVDAEPPVAALSGTSRPLRALLELARRVAASAAPVVIHGEPGVGKALLARFVHARSPRALGPFIVVDCRE
PADAVEEALFGRASAPGQPPVVSALLRADGGSLVLLHVESLARPASERLARALARRSAPARQGGEEPVDVRVLATAPASV
SLLAARGDVEASLARALSGFELDAPPIRERRADVLPLLEQFAARAARRIRKEPPTLSPEARRLLTEYGWPQNLRELELVG
ERLGLLYAASRVGALQLPPEFQEGGDEGAKTLQGRVARLERDAIAEALREASGKKVRAAALLGISRPTLDKKIEEYGLAV
ERGRRE

Sequences:

>Translated_566_residues
MPALLLLTGPSAGRRHDVLAEATIGRSPSCEIPLDDHQVSRKHALISVLDGQARIRDLRSRNGTFVNGERLVDEVVLKPG
DQVRVGVTTALFEPPPVTLVADGPEKMGQVPIEEVLPHVGAAAAMYSAGIALLGATSGALVLRRLADEVAHALNADRAAA
LLGSSDGLLTAAVAGAEALTMPRALAQAALERKELVQTDDVLCAPLVASGGMPFGVLYVTRAEPKFSEGEGQLLAALGRL
GGEAYTTVRSRVDAEPPVAALSGTSRPLRALLELARRVAASAAPVVIHGEPGVGKALLARFVHARSPRALGPFIVVDCRE
PADAVEEALFGRASAPGQPPVVSALLRADGGSLVLLHVESLARPASERLARALARRSAPARQGGEEPVDVRVLATAPASV
SLLAARGDVEASLARALSGFELDAPPIRERRADVLPLLEQFAARAARRIRKEPPTLSPEARRLLTEYGWPQNLRELELVG
ERLGLLYAASRVGALQLPPEFQEGGDEGAKTLQGRVARLERDAIAEALREASGKKVRAAALLGISRPTLDKKIEEYGLAV
ERGRRE
>Mature_565_residues
PALLLLTGPSAGRRHDVLAEATIGRSPSCEIPLDDHQVSRKHALISVLDGQARIRDLRSRNGTFVNGERLVDEVVLKPGD
QVRVGVTTALFEPPPVTLVADGPEKMGQVPIEEVLPHVGAAAAMYSAGIALLGATSGALVLRRLADEVAHALNADRAAAL
LGSSDGLLTAAVAGAEALTMPRALAQAALERKELVQTDDVLCAPLVASGGMPFGVLYVTRAEPKFSEGEGQLLAALGRLG
GEAYTTVRSRVDAEPPVAALSGTSRPLRALLELARRVAASAAPVVIHGEPGVGKALLARFVHARSPRALGPFIVVDCREP
ADAVEEALFGRASAPGQPPVVSALLRADGGSLVLLHVESLARPASERLARALARRSAPARQGGEEPVDVRVLATAPASVS
LLAARGDVEASLARALSGFELDAPPIRERRADVLPLLEQFAARAARRIRKEPPTLSPEARRLLTEYGWPQNLRELELVGE
RLGLLYAASRVGALQLPPEFQEGGDEGAKTLQGRVARLERDAIAEALREASGKKVRAAALLGISRPTLDKKIEEYGLAVE
RGRRE

Specific function: AnfA is essential for nitrogen fixation under Mo- and V- deficient conditions. It is required for the regulation of nitrogenase 3 transcription. Interacts with sigma-54 [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=307, Percent_Identity=31.5960912052117, Blast_Score=142, Evalue=7e-35,
Organism=Escherichia coli, GI1790299, Length=340, Percent_Identity=31.1764705882353, Blast_Score=126, Evalue=4e-30,
Organism=Escherichia coli, GI87081858, Length=305, Percent_Identity=31.8032786885246, Blast_Score=123, Evalue=3e-29,
Organism=Escherichia coli, GI1789233, Length=332, Percent_Identity=29.8192771084337, Blast_Score=122, Evalue=5e-29,
Organism=Escherichia coli, GI1790437, Length=318, Percent_Identity=31.7610062893082, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI1787583, Length=346, Percent_Identity=27.7456647398844, Blast_Score=119, Evalue=8e-28,
Organism=Escherichia coli, GI87082117, Length=322, Percent_Identity=31.055900621118, Blast_Score=111, Evalue=1e-25,
Organism=Escherichia coli, GI87081872, Length=230, Percent_Identity=33.4782608695652, Blast_Score=98, Evalue=2e-21,
Organism=Escherichia coli, GI1786524, Length=304, Percent_Identity=31.5789473684211, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI87082152, Length=222, Percent_Identity=35.1351351351351, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI1788905, Length=293, Percent_Identity=28.6689419795222, Blast_Score=92, Evalue=1e-19,
Organism=Escherichia coli, GI1789087, Length=399, Percent_Identity=28.8220551378446, Blast_Score=92, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR003018
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078 [H]

Pfam domain/function: PF01590 GAF; PF02954 HTH_8; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 59659; Mature: 59528

Theoretical pI: Translated: 8.11; Mature: 8.11

Prosite motif: PS50006 FHA_DOMAIN ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPALLLLTGPSAGRRHDVLAEATIGRSPSCEIPLDDHQVSRKHALISVLDGQARIRDLRS
CCEEEEEECCCCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHH
RNGTFVNGERLVDEVVLKPGDQVRVGVTTALFEPPPVTLVADGPEKMGQVPIEEVLPHVG
CCCCEECHHHHHHHHHHCCCCCEEEEEEEEEECCCCEEEEECCHHHHCCCCHHHHHHHHH
AAAAMYSAGIALLGATSGALVLRRLADEVAHALNADRAAALLGSSDGLLTAAVAGAEALT
HHHHHHHCCCCEEECCHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCHHHHHHCHHHHH
MPRALAQAALERKELVQTDDVLCAPLVASGGMPFGVLYVTRAEPKFSEGEGQLLAALGRL
HHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCEEEEEEEECCCCCCCCCHHHHHHHHHH
GGEAYTTVRSRVDAEPPVAALSGTSRPLRALLELARRVAASAAPVVIHGEPGVGKALLAR
CCHHHHHHHHHCCCCCCCHHHCCCCHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHH
FVHARSPRALGPFIVVDCREPADAVEEALFGRASAPGQPPVVSALLRADGGSLVLLHVES
HHHCCCCCCCCCEEEEECCCHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCEEEEEEHH
LARPASERLARALARRSAPARQGGEEPVDVRVLATAPASVSLLAARGDVEASLARALSGF
HCCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCEEEEEECCCHHHHHHHHHCCC
ELDAPPIRERRADVLPLLEQFAARAARRIRKEPPTLSPEARRLLTEYGWPQNLRELELVG
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCHHHHHHHH
ERLGLLYAASRVGALQLPPEFQEGGDEGAKTLQGRVARLERDAIAEALREASGKKVRAAA
HHHHHHHHHHCCCCCCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHH
LLGISRPTLDKKIEEYGLAVERGRRE
HHCCCCCCHHHHHHHHCHHHHCCCCC
>Mature Secondary Structure 
PALLLLTGPSAGRRHDVLAEATIGRSPSCEIPLDDHQVSRKHALISVLDGQARIRDLRS
CEEEEEECCCCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHH
RNGTFVNGERLVDEVVLKPGDQVRVGVTTALFEPPPVTLVADGPEKMGQVPIEEVLPHVG
CCCCEECHHHHHHHHHHCCCCCEEEEEEEEEECCCCEEEEECCHHHHCCCCHHHHHHHHH
AAAAMYSAGIALLGATSGALVLRRLADEVAHALNADRAAALLGSSDGLLTAAVAGAEALT
HHHHHHHCCCCEEECCHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCHHHHHHCHHHHH
MPRALAQAALERKELVQTDDVLCAPLVASGGMPFGVLYVTRAEPKFSEGEGQLLAALGRL
HHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCEEEEEEEECCCCCCCCCHHHHHHHHHH
GGEAYTTVRSRVDAEPPVAALSGTSRPLRALLELARRVAASAAPVVIHGEPGVGKALLAR
CCHHHHHHHHHCCCCCCCHHHCCCCHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHH
FVHARSPRALGPFIVVDCREPADAVEEALFGRASAPGQPPVVSALLRADGGSLVLLHVES
HHHCCCCCCCCCEEEEECCCHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCEEEEEEHH
LARPASERLARALARRSAPARQGGEEPVDVRVLATAPASVSLLAARGDVEASLARALSGF
HCCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCEEEEEECCCHHHHHHHHHCCC
ELDAPPIRERRADVLPLLEQFAARAARRIRKEPPTLSPEARRLLTEYGWPQNLRELELVG
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCHHHHHHHH
ERLGLLYAASRVGALQLPPEFQEGGDEGAKTLQGRVARLERDAIAEALREASGKKVRAAA
HHHHHHHHHHCCCCCCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHH
LLGISRPTLDKKIEEYGLAVERGRRE
HHCCCCCCHHHHHHHHCHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2722750 [H]