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The map label for this gene is walK [H]

Identifier: 108756850

GI number: 108756850

Start: 742253

End: 743962

Strand: Reverse

Name: walK [H]

Synonym: MXAN_0643

Alternate gene names: 108756850

Gene position: 743962-742253 (Counterclockwise)

Preceding gene: 108760945

Following gene: 108758080

Centisome position: 8.14

GC content: 68.25

Gene sequence:

>1710_bases
TTGGCGTCCGCACGCCGACCATCGACGTTGACGGCGCGGGCTGTGCCTCCAATACCTACTTGTATGGAAAGGGACACGCC
CTCCGCATTTCGCGTCGAGGCGTTTCGAGCTCCCATGTCCCGGGACGCTGGCAACGACGACCAGCGGGTTTCCGAGGAGA
GCATGGCCTCAGGCATCGACGAACTCAGCGCGTGGCTGGACGCCGCCGTGGACCCTTTCGTGGCCTGCGACGCCGGTGAG
AACGTCTGCTTTCTCAACGCCGCCGCGGAGCGCCTGCTGGGATGGACGCGAGAGGAGCTCATCGGCCAGCCGGCGTCCCG
GCTCTTTCCCCAGCGGCTGCACCGCCACGAAGGCGTGAGCCTCCTGCGCCACCTGCTCTCCCGGCGGGCCGCGCTCGGAG
GGCGAGCGACCCGGGTGCTCGCACGTCGCAAGGACGGCGCTGAAATCATGGTGGAGCTGACGGTGGGCGCCTCCGGCAAA
GGCCGGGATGAGCGCATCGTCCTCAACTTCCGCCGCCTGCACGAGGTCGTCGACACCCTGGCCGAGCCCGTGGAGCGCAC
GCTGCACGGACTCGAGTCGGAGAGCGTTCCTGGCGACGCGCTCTTCCGGACCGTCGTGGAGCACGCGCCCATGGGAATCA
TCTACTTCGACCGGAGTGCCATCGTCATCGCGTGCAACGAGCTGTTCGTGAGCATCATCGGCTCACCCAAGCGCTTGTTG
GTGGGCCTCAACCTCCTGTCGCTCCGGGATGAGGGCATCCTCCACTGCGTCCGGGAGACGCTGGCGGGACATACCTGCGA
GTACGAAGGCGAGTACCGCGCCATCACCTCAGGCAGGAAGACGCCGGTCCACGTGCGCTTCGCCCCCTGCTTCAACGCGG
CCGGACAGGTGGAGGGCGGCATTGGCATCGTCGAGGACATCTCCGAGCGCCGCCGCGCGGAGCGCGAGCGCGAGCGCCTG
TACCGTGAAGCCCAGGAGGCCATCCGCGTGCGCGACGACTTCCTGTCCATCGCCTCGCACGAGCTGAAGACACCCCTCAC
CCCGCTGAGCCTCCGGCTGGCCACGCTGGAGCGCCGGCTGGCGCGTGGCGAGCTCGTGGAGTCCTCCACCTTGCGTCAGG
CGCGCCTGTACCTCTTGCGCATCACCAGCCTCATCAACGACTTGCTGGACTCCTCACGCATCGAGGCGGGACGACTGGCC
CTTCACCGGGAGGCCACCCGGCTGGAAGGACTGGTGGAGCACGTCCTCCACGAGCTCGAGCCCCAGCGCGGCAACCACAC
CGTGCGGTTCGACGCGCCCGAGCAGCCCGTGCAGGTGAACGTGGACCCCTTCCGGATGGAGCAGGTGCTCGCCAATCTGG
TGGAGAACGCGTTCAAGTACAGCCCGAATGGCGGCACCGTGCGCGTGAGCTTGCGCCAGCGCGGCGGGCTGGCGCTCCTG
TCTGTCTCCGACGAAGGCATCGGCATCCCTCCGGACCAGCAGAAGCTGCTCTTCGACCGGTACTTCCGGGCGCGCAACGC
CTCCGCCCATTCCTTCGGTGGACTGGGGCTGGGGCTCTACATCAGCCGGGACATCGTCGAGCGCCACGGCGGGCGCATCT
GGGTGGAGAGCGAGCCAGGCCATGGCTCCACCTTCCACGTCGCCCTGCCCTTGATGGCGGGCGCTCCCGCGCAGCCGAAC
GTCGAGGAACCCGGACAGCTCATCCACTGA

Upstream 100 bases:

>100_bases
CACTGCGCGCACGAAGACAGCCGCACGCAAGGCGGGTGGCCGGAAACGCAAGGCCACGCGCCGCTAGACAGGGGCCCGCT
CGCTCGCCTGCACGTCGAAC

Downstream 100 bases:

>100_bases
AACTCCGCCACCCCGCCTTCCGCAAAAATGACAGGTGTGGGTGTCTTCCATTGGCCAAGAGGCGGGGGGAAGCGCCTGGA
TTCGCACGCTTCCGGCGCTG

Product: sensory box histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 569; Mature: 568

Protein sequence:

>569_residues
MASARRPSTLTARAVPPIPTCMERDTPSAFRVEAFRAPMSRDAGNDDQRVSEESMASGIDELSAWLDAAVDPFVACDAGE
NVCFLNAAAERLLGWTREELIGQPASRLFPQRLHRHEGVSLLRHLLSRRAALGGRATRVLARRKDGAEIMVELTVGASGK
GRDERIVLNFRRLHEVVDTLAEPVERTLHGLESESVPGDALFRTVVEHAPMGIIYFDRSAIVIACNELFVSIIGSPKRLL
VGLNLLSLRDEGILHCVRETLAGHTCEYEGEYRAITSGRKTPVHVRFAPCFNAAGQVEGGIGIVEDISERRRAERERERL
YREAQEAIRVRDDFLSIASHELKTPLTPLSLRLATLERRLARGELVESSTLRQARLYLLRITSLINDLLDSSRIEAGRLA
LHREATRLEGLVEHVLHELEPQRGNHTVRFDAPEQPVQVNVDPFRMEQVLANLVENAFKYSPNGGTVRVSLRQRGGLALL
SVSDEGIGIPPDQQKLLFDRYFRARNASAHSFGGLGLGLYISRDIVERHGGRIWVESEPGHGSTFHVALPLMAGAPAQPN
VEEPGQLIH

Sequences:

>Translated_569_residues
MASARRPSTLTARAVPPIPTCMERDTPSAFRVEAFRAPMSRDAGNDDQRVSEESMASGIDELSAWLDAAVDPFVACDAGE
NVCFLNAAAERLLGWTREELIGQPASRLFPQRLHRHEGVSLLRHLLSRRAALGGRATRVLARRKDGAEIMVELTVGASGK
GRDERIVLNFRRLHEVVDTLAEPVERTLHGLESESVPGDALFRTVVEHAPMGIIYFDRSAIVIACNELFVSIIGSPKRLL
VGLNLLSLRDEGILHCVRETLAGHTCEYEGEYRAITSGRKTPVHVRFAPCFNAAGQVEGGIGIVEDISERRRAERERERL
YREAQEAIRVRDDFLSIASHELKTPLTPLSLRLATLERRLARGELVESSTLRQARLYLLRITSLINDLLDSSRIEAGRLA
LHREATRLEGLVEHVLHELEPQRGNHTVRFDAPEQPVQVNVDPFRMEQVLANLVENAFKYSPNGGTVRVSLRQRGGLALL
SVSDEGIGIPPDQQKLLFDRYFRARNASAHSFGGLGLGLYISRDIVERHGGRIWVESEPGHGSTFHVALPLMAGAPAQPN
VEEPGQLIH
>Mature_568_residues
ASARRPSTLTARAVPPIPTCMERDTPSAFRVEAFRAPMSRDAGNDDQRVSEESMASGIDELSAWLDAAVDPFVACDAGEN
VCFLNAAAERLLGWTREELIGQPASRLFPQRLHRHEGVSLLRHLLSRRAALGGRATRVLARRKDGAEIMVELTVGASGKG
RDERIVLNFRRLHEVVDTLAEPVERTLHGLESESVPGDALFRTVVEHAPMGIIYFDRSAIVIACNELFVSIIGSPKRLLV
GLNLLSLRDEGILHCVRETLAGHTCEYEGEYRAITSGRKTPVHVRFAPCFNAAGQVEGGIGIVEDISERRRAERERERLY
REAQEAIRVRDDFLSIASHELKTPLTPLSLRLATLERRLARGELVESSTLRQARLYLLRITSLINDLLDSSRIEAGRLAL
HREATRLEGLVEHVLHELEPQRGNHTVRFDAPEQPVQVNVDPFRMEQVLANLVENAFKYSPNGGTVRVSLRQRGGLALLS
VSDEGIGIPPDQQKLLFDRYFRARNASAHSFGGLGLGLYISRDIVERHGGRIWVESEPGHGSTFHVALPLMAGAPAQPNV
EEPGQLIH

Specific function: Member of the two-component regulatory system walK/walR that regulates genes involved in autolysis, biofilm formation and cell wall metabolism. WalK functions as a sensor protein kinase which is autophosphorylated at a histidine residue and transfers its

COG id: COG5002

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI145693157, Length=368, Percent_Identity=29.6195652173913, Blast_Score=135, Evalue=1e-32,
Organism=Escherichia coli, GI1786912, Length=233, Percent_Identity=36.0515021459227, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI48994928, Length=377, Percent_Identity=27.8514588859416, Blast_Score=122, Evalue=9e-29,
Organism=Escherichia coli, GI1789149, Length=243, Percent_Identity=33.3333333333333, Blast_Score=111, Evalue=1e-25,
Organism=Escherichia coli, GI1788393, Length=226, Percent_Identity=33.6283185840708, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1788713, Length=257, Percent_Identity=27.6264591439689, Blast_Score=99, Evalue=6e-22,
Organism=Escherichia coli, GI1790436, Length=247, Percent_Identity=31.9838056680162, Blast_Score=92, Evalue=8e-20,
Organism=Escherichia coli, GI1790346, Length=220, Percent_Identity=32.2727272727273, Blast_Score=92, Evalue=9e-20,
Organism=Escherichia coli, GI1788549, Length=354, Percent_Identity=26.5536723163842, Blast_Score=89, Evalue=1e-18,
Organism=Escherichia coli, GI1786600, Length=223, Percent_Identity=30.4932735426009, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI87081816, Length=254, Percent_Identity=31.1023622047244, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1786783, Length=223, Percent_Identity=27.8026905829596, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI87082128, Length=228, Percent_Identity=29.8245614035088, Blast_Score=78, Evalue=2e-15,
Organism=Escherichia coli, GI1787894, Length=218, Percent_Identity=30.7339449541284, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1790300, Length=356, Percent_Identity=26.9662921348315, Blast_Score=76, Evalue=7e-15,
Organism=Escherichia coli, GI1790551, Length=275, Percent_Identity=25.8181818181818, Blast_Score=69, Evalue=9e-13,
Organism=Escherichia coli, GI1789808, Length=246, Percent_Identity=26.8292682926829, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR000014
- InterPro:   IPR000700
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 63085; Mature: 62954

Theoretical pI: Translated: 6.93; Mature: 6.93

Prosite motif: PS50112 PAS ; PS50113 PAC ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASARRPSTLTARAVPPIPTCMERDTPSAFRVEAFRAPMSRDAGNDDQRVSEESMASGID
CCCCCCCCCEEECCCCCCCHHHCCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCHH
ELSAWLDAAVDPFVACDAGENVCFLNAAAERLLGWTREELIGQPASRLFPQRLHRHEGVS
HHHHHHHHHCCCEEEECCCCCEEEEHHHHHHHHCCCHHHHCCCCHHHHHHHHHHHHCCHH
LLRHLLSRRAALGGRATRVLARRKDGAEIMVELTVGASGKGRDERIVLNFRRLHEVVDTL
HHHHHHHHHHHCCCHHHHHHHHCCCCCEEEEEEEECCCCCCCCCEEEEHHHHHHHHHHHH
AEPVERTLHGLESESVPGDALFRTVVEHAPMGIIYFDRSAIVIACNELFVSIIGSPKRLL
HHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCEEEEECCCEEEEEHHHHHHHHHCCCHHHH
VGLNLLSLRDEGILHCVRETLAGHTCEYEGEYRAITSGRKTPVHVRFAPCFNAAGQVEGG
HCCHHHHHCCHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCEEEEEECCCCCCCCCCCCC
IGIVEDISERRRAERERERLYREAQEAIRVRDDFLSIASHELKTPLTPLSLRLATLERRL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
ARGELVESSTLRQARLYLLRITSLINDLLDSSRIEAGRLALHREATRLEGLVEHVLHELE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
PQRGNHTVRFDAPEQPVQVNVDPFRMEQVLANLVENAFKYSPNGGTVRVSLRQRGGLALL
CCCCCEEEEECCCCCCEEECCCHHHHHHHHHHHHHHHHEECCCCCEEEEEEECCCCEEEE
SVSDEGIGIPPDQQKLLFDRYFRARNASAHSFGGLGLGLYISRDIVERHGGRIWVESEPG
EECCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCEEHHHHHHHHHHHCCCEEEEECCCC
HGSTFHVALPLMAGAPAQPNVEEPGQLIH
CCCEEEEEEEHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
ASARRPSTLTARAVPPIPTCMERDTPSAFRVEAFRAPMSRDAGNDDQRVSEESMASGID
CCCCCCCCEEECCCCCCCHHHCCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCHH
ELSAWLDAAVDPFVACDAGENVCFLNAAAERLLGWTREELIGQPASRLFPQRLHRHEGVS
HHHHHHHHHCCCEEEECCCCCEEEEHHHHHHHHCCCHHHHCCCCHHHHHHHHHHHHCCHH
LLRHLLSRRAALGGRATRVLARRKDGAEIMVELTVGASGKGRDERIVLNFRRLHEVVDTL
HHHHHHHHHHHCCCHHHHHHHHCCCCCEEEEEEEECCCCCCCCCEEEEHHHHHHHHHHHH
AEPVERTLHGLESESVPGDALFRTVVEHAPMGIIYFDRSAIVIACNELFVSIIGSPKRLL
HHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCEEEEECCCEEEEEHHHHHHHHHCCCHHHH
VGLNLLSLRDEGILHCVRETLAGHTCEYEGEYRAITSGRKTPVHVRFAPCFNAAGQVEGG
HCCHHHHHCCHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCEEEEEECCCCCCCCCCCCC
IGIVEDISERRRAERERERLYREAQEAIRVRDDFLSIASHELKTPLTPLSLRLATLERRL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
ARGELVESSTLRQARLYLLRITSLINDLLDSSRIEAGRLALHREATRLEGLVEHVLHELE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
PQRGNHTVRFDAPEQPVQVNVDPFRMEQVLANLVENAFKYSPNGGTVRVSLRQRGGLALL
CCCCCEEEEECCCCCCEEECCCHHHHHHHHHHHHHHHHEECCCCCEEEEEEECCCCEEEE
SVSDEGIGIPPDQQKLLFDRYFRARNASAHSFGGLGLGLYISRDIVERHGGRIWVESEPG
EECCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCEEHHHHHHHHHHHCCCEEEEECCCC
HGSTFHVALPLMAGAPAQPNVEEPGQLIH
CCCEEEEEEEHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA