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The map label for this gene is stp [H]

Identifier: 108756840

GI number: 108756840

Start: 346571

End: 347986

Strand: Reverse

Name: stp [H]

Synonym: MXAN_0292

Alternate gene names: 108756840

Gene position: 347986-346571 (Counterclockwise)

Preceding gene: 108759219

Following gene: 108760444

Centisome position: 3.81

GC content: 73.02

Gene sequence:

>1416_bases
ATGCCCACCAACGCCCCCGACACCACCGTCGCCTATGACAGTCCTCGCGGCCGGGGTGTGCTGCTCGCCAGCGTGCTGGG
CAGCGGCATGGCGTTCCTCGATTCCACCGCCGTCAACGTCGCCCTGCCCGCCATGGGCCGCGAGCTGCACACGGGCCTGT
CCGGCCTCCAATGGGCCGTGGACGCGTACCTGCTCACGCTCGGCTCCCTGGTGCTCACCGGGGGCGCGCTCGGCGACGCG
AAGGGCCAGCGACGCGTCTTCCTGCTGGGCATGCTCGCCTTCACCGTCACGTCAGTGCTGTGCGGCCTGGCGCCCAACAC
CTGGACGCTGGCCGCCTTCCGCGCGGCGCAGGGTGTGGGAGCCGCGCTGCTGGTGCCCGCGAGCCTCGCATTGCTGCGCA
CGTCGTTCCCGCCTCAGGACCGGCAACGCGCCGTGTCCGCCTGGGCCGGACTGTCCGGCGTCACCACGGCCGTGGGGCCG
CTGCTGGGTGGGTGGCTCGTGGACGCAGGCTCATGGCGCTTCGTCTTCTTCCTCAACGTGCCCCTGGCCGCGCTCACCGC
GTGGGCGGCGCTGCGCCACGTGCCGGACATCGCGCCGAAGACAGGGGCGCGCGGGTTGGACGTCGCGGGCTCGGTGACGG
CGGCGCTCGGGCTGGGTGGCCTCATCTACGCGCTCATCGAAGGCCCCGCCCACGGCTGGCCCCTGGCGGCCATCGGCGCC
GCGGCCGGAGGCGCCGTCCTGCTGGCCGCGTTCTTCGTCCTGGAGGCACGGCAGCGGGAGCCGATGCTTCCGCTCACCCT
CTTCCACTCGCGCACCTTCTCCGGCGCGAACCTCACCACGCTCGTCGTGTACTTCGCGCTCGGAGGCGCCACCTTCCTGG
TGGTGCTCGCCCTGCAGCAGCAGCTCGGCTATTCGGCGCTCGGCGCGGGAGCAGCGCTGCTCCCCATCACGCTGATGCTG
CTTCTGTTTTCACCTTCGGTGGGGCGGCTCGCGGGGCACATCGGCGCACGTCCGTTGATGACGGCGGGGCCGCTGCTCGC
GGGCGTGGGGCTGGCGCTCCTGACGCGCATCCAGCGCGGTGGTGACTACGTCAGCACCGTGCTGCCTGGCATCCTGGTGC
TGGGACTGGGGCTGGCGCTCACCGTAGGCCCGCTCACGGCGGTGGTGCTCGGCGCCGTCGAGGACCGCTACGCGGGCATC
GCCTCTGGCGTGAACAACGCCGTGGCCCGCCTCGCCGGCCTGCTCGCGGTGGCGCTGCTGCCGCTGCTGGGCGGCCTCGC
TGGCGACACCGGCATGGACTTCCTGGTGGGCACACGGCGCGCGCTCTGGGTGTCCGCCGGCCTGTGCGCCGTGGGAGCGC
TGTGCTCGCTGCTCACGATTCCACGCGAGGCCGGGCGCACGACCTCCGCGCAATAG

Upstream 100 bases:

>100_bases
TTCAGTGAGCACCAGCCAGGATGGCGAGCCACTGGAGGCACGTCCCCACCCCGCAGCAGCGGATGGCACGCGCGGAGCCC
CGGGCGCACGGTAGAGCCAC

Downstream 100 bases:

>100_bases
GAGCACGGGACCAAGCGCACCGCGTGGGTCCAACAAGCGCTCATGACGTCGTCGCGGGATGGCGCCTCGGCCCCCGGCTG
GCTTCTTCGCGGCCTTGCCG

Product: EmrB/QacA family drug resistance transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Spectinomycin tetracycline efflux pump [H]

Number of amino acids: Translated: 471; Mature: 470

Protein sequence:

>471_residues
MPTNAPDTTVAYDSPRGRGVLLASVLGSGMAFLDSTAVNVALPAMGRELHTGLSGLQWAVDAYLLTLGSLVLTGGALGDA
KGQRRVFLLGMLAFTVTSVLCGLAPNTWTLAAFRAAQGVGAALLVPASLALLRTSFPPQDRQRAVSAWAGLSGVTTAVGP
LLGGWLVDAGSWRFVFFLNVPLAALTAWAALRHVPDIAPKTGARGLDVAGSVTAALGLGGLIYALIEGPAHGWPLAAIGA
AAGGAVLLAAFFVLEARQREPMLPLTLFHSRTFSGANLTTLVVYFALGGATFLVVLALQQQLGYSALGAGAALLPITLML
LLFSPSVGRLAGHIGARPLMTAGPLLAGVGLALLTRIQRGGDYVSTVLPGILVLGLGLALTVGPLTAVVLGAVEDRYAGI
ASGVNNAVARLAGLLAVALLPLLGGLAGDTGMDFLVGTRRALWVSAGLCAVGALCSLLTIPREAGRTTSAQ

Sequences:

>Translated_471_residues
MPTNAPDTTVAYDSPRGRGVLLASVLGSGMAFLDSTAVNVALPAMGRELHTGLSGLQWAVDAYLLTLGSLVLTGGALGDA
KGQRRVFLLGMLAFTVTSVLCGLAPNTWTLAAFRAAQGVGAALLVPASLALLRTSFPPQDRQRAVSAWAGLSGVTTAVGP
LLGGWLVDAGSWRFVFFLNVPLAALTAWAALRHVPDIAPKTGARGLDVAGSVTAALGLGGLIYALIEGPAHGWPLAAIGA
AAGGAVLLAAFFVLEARQREPMLPLTLFHSRTFSGANLTTLVVYFALGGATFLVVLALQQQLGYSALGAGAALLPITLML
LLFSPSVGRLAGHIGARPLMTAGPLLAGVGLALLTRIQRGGDYVSTVLPGILVLGLGLALTVGPLTAVVLGAVEDRYAGI
ASGVNNAVARLAGLLAVALLPLLGGLAGDTGMDFLVGTRRALWVSAGLCAVGALCSLLTIPREAGRTTSAQ
>Mature_470_residues
PTNAPDTTVAYDSPRGRGVLLASVLGSGMAFLDSTAVNVALPAMGRELHTGLSGLQWAVDAYLLTLGSLVLTGGALGDAK
GQRRVFLLGMLAFTVTSVLCGLAPNTWTLAAFRAAQGVGAALLVPASLALLRTSFPPQDRQRAVSAWAGLSGVTTAVGPL
LGGWLVDAGSWRFVFFLNVPLAALTAWAALRHVPDIAPKTGARGLDVAGSVTAALGLGGLIYALIEGPAHGWPLAAIGAA
AGGAVLLAAFFVLEARQREPMLPLTLFHSRTFSGANLTTLVVYFALGGATFLVVLALQQQLGYSALGAGAALLPITLMLL
LFSPSVGRLAGHIGARPLMTAGPLLAGVGLALLTRIQRGGDYVSTVLPGILVLGLGLALTVGPLTAVVLGAVEDRYAGIA
SGVNNAVARLAGLLAVALLPLLGGLAGDTGMDFLVGTRRALWVSAGLCAVGALCSLLTIPREAGRTTSAQ

Specific function: Contributes to spectinomycin and tetracycline resistance [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1788710, Length=409, Percent_Identity=29.3398533007335, Blast_Score=149, Evalue=5e-37,
Organism=Escherichia coli, GI1789042, Length=391, Percent_Identity=28.9002557544757, Blast_Score=146, Evalue=3e-36,
Organism=Escherichia coli, GI87081983, Length=420, Percent_Identity=29.2857142857143, Blast_Score=137, Evalue=2e-33,
Organism=Escherichia coli, GI1790195, Length=414, Percent_Identity=28.0193236714976, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI1788392, Length=455, Percent_Identity=28.3516483516484, Blast_Score=108, Evalue=8e-25,
Organism=Saccharomyces cerevisiae, GI6324954, Length=433, Percent_Identity=23.3256351039261, Blast_Score=89, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6323935, Length=493, Percent_Identity=23.5294117647059, Blast_Score=78, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6323520, Length=434, Percent_Identity=21.6589861751152, Blast_Score=72, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 47681; Mature: 47550

Theoretical pI: Translated: 10.13; Mature: 10.13

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTNAPDTTVAYDSPRGRGVLLASVLGSGMAFLDSTAVNVALPAMGRELHTGLSGLQWAV
CCCCCCCCEEEECCCCCCCEEHHHHHCCCHHHHHCCHHHEEHHHCCHHHHHHHHHHHHHH
DAYLLTLGSLVLTGGALGDAKGQRRVFLLGMLAFTVTSVLCGLAPNTWTLAAFRAAQGVG
HHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCC
AALLVPASLALLRTSFPPQDRQRAVSAWAGLSGVTTAVGPLLGGWLVDAGSWRFVFFLNV
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHEECCCEEEEEEECC
PLAALTAWAALRHVPDIAPKTGARGLDVAGSVTAALGLGGLIYALIEGPAHGWPLAAIGA
HHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHH
AAGGAVLLAAFFVLEARQREPMLPLTLFHSRTFSGANLTTLVVYFALGGATFLVVLALQQ
CCCHHHHHHHHHHHHHHCCCCCCCEEEHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
QLGYSALGAGAALLPITLMLLLFSPSVGRLAGHIGARPLMTAGPLLAGVGLALLTRIQRG
HHCHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHC
GDYVSTVLPGILVLGLGLALTVGPLTAVVLGAVEDRYAGIASGVNNAVARLAGLLAVALL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PLLGGLAGDTGMDFLVGTRRALWVSAGLCAVGALCSLLTIPREAGRTTSAQ
HHHCCCCCCCCCHHHHHCHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCC
>Mature Secondary Structure 
PTNAPDTTVAYDSPRGRGVLLASVLGSGMAFLDSTAVNVALPAMGRELHTGLSGLQWAV
CCCCCCCEEEECCCCCCCEEHHHHHCCCHHHHHCCHHHEEHHHCCHHHHHHHHHHHHHH
DAYLLTLGSLVLTGGALGDAKGQRRVFLLGMLAFTVTSVLCGLAPNTWTLAAFRAAQGVG
HHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCC
AALLVPASLALLRTSFPPQDRQRAVSAWAGLSGVTTAVGPLLGGWLVDAGSWRFVFFLNV
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHEECCCEEEEEEECC
PLAALTAWAALRHVPDIAPKTGARGLDVAGSVTAALGLGGLIYALIEGPAHGWPLAAIGA
HHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHH
AAGGAVLLAAFFVLEARQREPMLPLTLFHSRTFSGANLTTLVVYFALGGATFLVVLALQQ
CCCHHHHHHHHHHHHHHCCCCCCCEEEHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
QLGYSALGAGAALLPITLMLLLFSPSVGRLAGHIGARPLMTAGPLLAGVGLALLTRIQRG
HHCHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHC
GDYVSTVLPGILVLGLGLALTVGPLTAVVLGAVEDRYAGIASGVNNAVARLAGLLAVALL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PLLGGLAGDTGMDFLVGTRRALWVSAGLCAVGALCSLLTIPREAGRTTSAQ
HHHCCCCCCCCCHHHHHCHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]