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The map label for this gene is stsIM [H]

Identifier: 108563838

GI number: 108563838

Start: 1470989

End: 1471888

Strand: Reverse

Name: stsIM [H]

Synonym: HPAG1_1413

Alternate gene names: 108563838

Gene position: 1471888-1470989 (Counterclockwise)

Preceding gene: 108563839

Following gene: 108563837

Centisome position: 92.2

GC content: 28.11

Gene sequence:

>900_bases
TTGAAAAAAGGTATTCGTTCTCCCTTTTTCTATGTAGGGGATAAATATAAACTCATGCCGCAACTCAATAAGCTATTCCC
AAATAACATTAATCAATTTATTGAGCCTTTTGTGGGTGGGGGTAGCGTGTTTTTAAACACTAAGGCTAAAAGATACCTAG
CTAACGACATAGATACCAATATTATCAATTTGCATAAAACTTTAAGCAAGTTCAATACTTGTGAGCTTTTTGATGAATTG
TCTAAAATCATCATTCATTATGGCTTGTCTTTCTCTTTTAAGGGGATTACAGCCCCTGATGAATTAAAAAAACAATATAT
AAAAACTTACTACGCCAAATACAATAAAATAGCTTATGAAAAACTAAGGGCTGATTTTAACTCCAATCAAAACAACATGC
TCTATTTGTATTTACTTTTAATTTATGGGTTTAATCACATGATTAGATTTAATTCTAAAGGGCTTTTTAATTTACCTGTA
GGTAATGTAGATTTTAATGAAAATGTTTATAATGCCCTAAAAAACTACATAGATTTTATACAGCAAAACACCATTATTTT
TTATAATGGCGATTATATTGATTTCCTTAACCACACCACTTATTTAAAAGATGATTATGTTTATTTTGACCCCCCTTATT
TAATCTCAAATAGTGAATACAACAAGTTATGGGATAGCGATAATGAGATAGCCTTATATGGTGTTTTAGATAACCTAGAT
AAAAAGGGAGTTTTATTTGGTATAACTAATCTTGTTTATCACAAGGGAGAGACTAATTTTATTTTAAAAGAATGGGCTAA
AAAATATTATATTTTTAATATCAAAAGTAATTATATCAGTTATAATGACAATACCATTAAAGAAAATAGTCAAGAAATCT
TTGTAACTAATTATAGGTGA

Upstream 100 bases:

>100_bases
GAAAATTAAGCGTTAAAGAAAAGGCTCATGGTTTTTTTAATTCAGGAAAAACTGATTTTAAAGAACATAAAGAATTTTTA
TTTATAGTGGAAGTGAAACC

Downstream 100 bases:

>100_bases
TTAATTATGACTAAAAAACCGGCACGAAAAATTTTAAGCTTTTCAACCACCATGCGAAACCCTAAAAGAATAGGGCAATT
TTTAGCTGTTTTAGGAAAGT

Product: type II adenine specific DNA methyltransferase

Products: NA

Alternate protein names: M.StsI; Adenine-specific methyltransferase StsI [H]

Number of amino acids: Translated: 299; Mature: 299

Protein sequence:

>299_residues
MKKGIRSPFFYVGDKYKLMPQLNKLFPNNINQFIEPFVGGGSVFLNTKAKRYLANDIDTNIINLHKTLSKFNTCELFDEL
SKIIIHYGLSFSFKGITAPDELKKQYIKTYYAKYNKIAYEKLRADFNSNQNNMLYLYLLLIYGFNHMIRFNSKGLFNLPV
GNVDFNENVYNALKNYIDFIQQNTIIFYNGDYIDFLNHTTYLKDDYVYFDPPYLISNSEYNKLWDSDNEIALYGVLDNLD
KKGVLFGITNLVYHKGETNFILKEWAKKYYIFNIKSNYISYNDNTIKENSQEIFVTNYR

Sequences:

>Translated_299_residues
MKKGIRSPFFYVGDKYKLMPQLNKLFPNNINQFIEPFVGGGSVFLNTKAKRYLANDIDTNIINLHKTLSKFNTCELFDEL
SKIIIHYGLSFSFKGITAPDELKKQYIKTYYAKYNKIAYEKLRADFNSNQNNMLYLYLLLIYGFNHMIRFNSKGLFNLPV
GNVDFNENVYNALKNYIDFIQQNTIIFYNGDYIDFLNHTTYLKDDYVYFDPPYLISNSEYNKLWDSDNEIALYGVLDNLD
KKGVLFGITNLVYHKGETNFILKEWAKKYYIFNIKSNYISYNDNTIKENSQEIFVTNYR
>Mature_299_residues
MKKGIRSPFFYVGDKYKLMPQLNKLFPNNINQFIEPFVGGGSVFLNTKAKRYLANDIDTNIINLHKTLSKFNTCELFDEL
SKIIIHYGLSFSFKGITAPDELKKQYIKTYYAKYNKIAYEKLRADFNSNQNNMLYLYLLLIYGFNHMIRFNSKGLFNLPV
GNVDFNENVYNALKNYIDFIQQNTIIFYNGDYIDFLNHTTYLKDDYVYFDPPYLISNSEYNKLWDSDNEIALYGVLDNLD
KKGVLFGITNLVYHKGETNFILKEWAKKYYIFNIKSNYISYNDNTIKENSQEIFVTNYR

Specific function: This methylase recognizes the double-stranded sequence 5'-GGATG-3' in one strand and 3'-CATCC-5' in the other, causes specific methylation on the A of both strands, and protects the DNA from cleavage by the StsI endonuclease. The 2 domains of the protein

COG id: COG0338

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789789, Length=301, Percent_Identity=25.2491694352159, Blast_Score=71, Evalue=9e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012186
- InterPro:   IPR023095
- InterPro:   IPR002052
- InterPro:   IPR012327 [H]

Pfam domain/function: PF02086 MethyltransfD12 [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 35356; Mature: 35356

Theoretical pI: Translated: 9.00; Mature: 9.00

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKGIRSPFFYVGDKYKLMPQLNKLFPNNINQFIEPFVGGGSVFLNTKAKRYLANDIDTN
CCCCCCCCEEEECCCEEECHHHHHHCCCCHHHHHHHHCCCCEEEEECHHHHHHHHCCCCH
IINLHKTLSKFNTCELFDELSKIIIHYGLSFSFKGITAPDELKKQYIKTYYAKYNKIAYE
HHHHHHHHHHCCHHHHHHHHHHHHHHHCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHH
KLRADFNSNQNNMLYLYLLLIYGFNHMIRFNSKGLFNLPVGNVDFNENVYNALKNYIDFI
HHHHHCCCCCCCCHHHHHHHHHCCCCEEEECCCCEEECCCCCCCCCHHHHHHHHHHHHHH
QQNTIIFYNGDYIDFLNHTTYLKDDYVYFDPPYLISNSEYNKLWDSDNEIALYGVLDNLD
HCCEEEEECCCEEEECCCCEEEECCEEEECCCEEECCCCCHHHCCCCCCEEEEEEECCCC
KKGVLFGITNLVYHKGETNFILKEWAKKYYIFNIKSNYISYNDNTIKENSQEIFVTNYR
CCCEEEEEHHHEEECCCCCHHHHHHCCEEEEEEEECCEEEECCCCCCCCCCEEEEEECC
>Mature Secondary Structure
MKKGIRSPFFYVGDKYKLMPQLNKLFPNNINQFIEPFVGGGSVFLNTKAKRYLANDIDTN
CCCCCCCCEEEECCCEEECHHHHHHCCCCHHHHHHHHCCCCEEEEECHHHHHHHHCCCCH
IINLHKTLSKFNTCELFDELSKIIIHYGLSFSFKGITAPDELKKQYIKTYYAKYNKIAYE
HHHHHHHHHHCCHHHHHHHHHHHHHHHCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHH
KLRADFNSNQNNMLYLYLLLIYGFNHMIRFNSKGLFNLPVGNVDFNENVYNALKNYIDFI
HHHHHCCCCCCCCHHHHHHHHHCCCCEEEECCCCEEECCCCCCCCCHHHHHHHHHHHHHH
QQNTIIFYNGDYIDFLNHTTYLKDDYVYFDPPYLISNSEYNKLWDSDNEIALYGVLDNLD
HCCEEEEECCCEEEECCCCEEEECCEEEECCCEEECCCCCHHHCCCCCCEEEEEEECCCC
KKGVLFGITNLVYHKGETNFILKEWAKKYYIFNIKSNYISYNDNTIKENSQEIFVTNYR
CCCEEEEEHHHEEECCCCCHHHHHHCCEEEEEEEECCEEEECCCCCCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1387204 [H]