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The map label for this gene is miaA

Identifier: 108563766

GI number: 108563766

Start: 1391720

End: 1392550

Strand: Direct

Name: miaA

Synonym: HPAG1_1341

Alternate gene names: 108563766

Gene position: 1391720-1392550 (Clockwise)

Preceding gene: 108563765

Following gene: 108563767

Centisome position: 87.18

GC content: 39.59

Gene sequence:

>831_bases
TTGGACGCTGAAATCTTTTCTTTGGATTCTTTGAGTATTTATAAAGACATTAATATCGCTTCGGCTAAACCGAGCCTGAA
AGAACGAAAAAACATCAAGCATTACGCCCTAGATCACCTTAACATTGATGAAAAAAATAACGCCCAGCTCTTTAAAACCC
TTTTAGAGGACGCCATGAGGGTGTCTTCTAAAGAAATTTTACTCATTGTGGGAGGGAGCAGTTTTTACCTAAAATCCATT
TTAGAAGGTTTGAGCCGCATGCCAAAAATTAGCGGTGAGGAGGTTGTAAAAATAGAGCGAGAAATCAGCACCCTAGCTGA
CCCTTACGCGTTTTTAAAATCCATTGACCCTACGATCGCTTTTAAAATCCATCCAAACGACACTTACCGCATCCATAAGG
CTTTAGAAATCTTTTATGCCACCCACACGCCCCCAAGCGAGTATTTTAAGGCCAACCCTAAAAAACCCTTTGAGCATGCC
ATCTCGTTATTCGCTCTGTCTATTGAAAAAAGCACGCTTCATAGCAACATCAAACAGCGCACCAAGAACATGCTCCATTC
AGGGCTTGTTGAAGAAATCAAAGCCCTCTATACCCAATACCCTAAAGATTCGCAGCCTTTTAAAGCCATAGGCGTTAAAG
AGAGTATTCTTTTTTTAGAAAAACAACTCACTTTAAAGGAGCTAGAAGAAGCGATTATCTCTAACACCATGAAATTAGCC
AAGTGCCAAAACACTTTCAATAAAACCCAATTCAATAACCTTTATGTAGGGAGCGTTAAAGAAGTTAGGCATGCGATTTT
AAACCACTCAAAAAGCGCTATTAAAGGATAA

Upstream 100 bases:

>100_bases
AAGAGGGAGAGCGTTCATCAAAACGCCTAAGAAACTCATCGCGCTTCTAGGGCCTAGCGGGAGCGGGAAAAGCGCTCTTT
CCATTGAATTAGCCCAAGAA

Downstream 100 bases:

>100_bases
CCTAATGGATACGCAAACTTTGCAAAATCAAATTATCCCTATTTTTATGAGTTTTGATAAGAATTATGCGCTAGGGGCTG
GCGTGAGCCTTTATTCTTTA

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase

Number of amino acids: Translated: 276; Mature: 276

Protein sequence:

>276_residues
MDAEIFSLDSLSIYKDINIASAKPSLKERKNIKHYALDHLNIDEKNNAQLFKTLLEDAMRVSSKEILLIVGGSSFYLKSI
LEGLSRMPKISGEEVVKIEREISTLADPYAFLKSIDPTIAFKIHPNDTYRIHKALEIFYATHTPPSEYFKANPKKPFEHA
ISLFALSIEKSTLHSNIKQRTKNMLHSGLVEEIKALYTQYPKDSQPFKAIGVKESILFLEKQLTLKELEEAIISNTMKLA
KCQNTFNKTQFNNLYVGSVKEVRHAILNHSKSAIKG

Sequences:

>Translated_276_residues
MDAEIFSLDSLSIYKDINIASAKPSLKERKNIKHYALDHLNIDEKNNAQLFKTLLEDAMRVSSKEILLIVGGSSFYLKSI
LEGLSRMPKISGEEVVKIEREISTLADPYAFLKSIDPTIAFKIHPNDTYRIHKALEIFYATHTPPSEYFKANPKKPFEHA
ISLFALSIEKSTLHSNIKQRTKNMLHSGLVEEIKALYTQYPKDSQPFKAIGVKESILFLEKQLTLKELEEAIISNTMKLA
KCQNTFNKTQFNNLYVGSVKEVRHAILNHSKSAIKG
>Mature_276_residues
MDAEIFSLDSLSIYKDINIASAKPSLKERKNIKHYALDHLNIDEKNNAQLFKTLLEDAMRVSSKEILLIVGGSSFYLKSI
LEGLSRMPKISGEEVVKIEREISTLADPYAFLKSIDPTIAFKIHPNDTYRIHKALEIFYATHTPPSEYFKANPKKPFEHA
ISLFALSIEKSTLHSNIKQRTKNMLHSGLVEEIKALYTQYPKDSQPFKAIGVKESILFLEKQLTLKELEEAIISNTMKLA
KCQNTFNKTQFNNLYVGSVKEVRHAILNHSKSAIKG

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A)

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family

Homologues:

Organism=Homo sapiens, GI31581534, Length=293, Percent_Identity=25.938566552901, Blast_Score=85, Evalue=8e-17,
Organism=Escherichia coli, GI1790613, Length=250, Percent_Identity=30.4, Blast_Score=115, Evalue=4e-27,
Organism=Caenorhabditis elegans, GI25144712, Length=248, Percent_Identity=25.8064516129032, Blast_Score=69, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6324848, Length=300, Percent_Identity=29, Blast_Score=105, Evalue=7e-24,
Organism=Drosophila melanogaster, GI24649830, Length=264, Percent_Identity=26.1363636363636, Blast_Score=74, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAA_HELPH (Q1CRL4)

Other databases:

- EMBL:   CP000241
- RefSeq:   YP_628082.1
- ProteinModelPortal:   Q1CRL4
- SMR:   Q1CRL4
- STRING:   Q1CRL4
- GeneID:   4098138
- GenomeReviews:   CP000241_GR
- KEGG:   hpa:HPAG1_1341
- eggNOG:   COG0324
- HOGENOM:   HBG559459
- OMA:   KEVRHAI
- ProtClustDB:   PRK00091
- BioCyc:   HPYL357544:HPAG1_1341-MONOMER
- HAMAP:   MF_00185
- InterPro:   IPR002627
- InterPro:   IPR018022
- PANTHER:   PTHR11088
- TIGRFAMs:   TIGR00174

Pfam domain/function: PF01715 IPPT

EC number: =2.5.1.75

Molecular weight: Translated: 31356; Mature: 31356

Theoretical pI: Translated: 9.69; Mature: 9.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDAEIFSLDSLSIYKDINIASAKPSLKERKNIKHYALDHLNIDEKNNAQLFKTLLEDAMR
CCCCEEECCCCHHHHCCCCCCCCCCHHHHHCCHHHHHCCCCCCCCCCHHHHHHHHHHHHH
VSSKEILLIVGGSSFYLKSILEGLSRMPKISGEEVVKIEREISTLADPYAFLKSIDPTIA
CCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEE
FKIHPNDTYRIHKALEIFYATHTPPSEYFKANPKKPFEHAISLFALSIEKSTLHSNIKQR
EEECCCCCHHHHHHHHHHHCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
TKNMLHSGLVEEIKALYTQYPKDSQPFKAIGVKESILFLEKQLTLKELEEAIISNTMKLA
HHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KCQNTFNKTQFNNLYVGSVKEVRHAILNHSKSAIKG
HHHHHHHHHHCCCEEECCHHHHHHHHHHCCHHHCCC
>Mature Secondary Structure
MDAEIFSLDSLSIYKDINIASAKPSLKERKNIKHYALDHLNIDEKNNAQLFKTLLEDAMR
CCCCEEECCCCHHHHCCCCCCCCCCHHHHHCCHHHHHCCCCCCCCCCHHHHHHHHHHHHH
VSSKEILLIVGGSSFYLKSILEGLSRMPKISGEEVVKIEREISTLADPYAFLKSIDPTIA
CCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEE
FKIHPNDTYRIHKALEIFYATHTPPSEYFKANPKKPFEHAISLFALSIEKSTLHSNIKQR
EEECCCCCHHHHHHHHHHHCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
TKNMLHSGLVEEIKALYTQYPKDSQPFKAIGVKESILFLEKQLTLKELEEAIISNTMKLA
HHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KCQNTFNKTQFNNLYVGSVKEVRHAILNHSKSAIKG
HHHHHHHHHHCCCEEECCHHHHHHHHHHCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA