Click here to switch to the map view.

The map label for this gene is trpA [H]

Identifier: 108563660

GI number: 108563660

Start: 1297524

End: 1298312

Strand: Direct

Name: trpA [H]

Synonym: HPAG1_1235

Alternate gene names: 108563660

Gene position: 1297524-1298312 (Clockwise)

Preceding gene: 108563659

Following gene: 108563661

Centisome position: 81.28

GC content: 40.18

Gene sequence:

>789_bases
ATGAGGTATCAAAACATGTTTGAAACCTTAAAAAAACAAGACAAAATGGCGTTTATCCCGTTTGTAACCTTGGGTGATCC
CAATTATGAATGGAGTTTTGAAATCATTAAAACCCTAATTGATAGCGGGGTGAGCGCTTTAGAATTGGGTTTTGCTTTTT
CCGATCCGGTAGCGGATGGCGTTACCATACAAGCGAGCCATTTAAGGGCGTTAAAACACGCTAGCATGGCTAAAAATTTC
CAGCTTTTAAGGGCGCTCAGAGATTACAATCCCCACATTCCCATAGGGCTTTTAGCGTATGCGAATCTCATTTTTTCTTA
TGGTGTTGATGGCTTTTACGCTCAAATCAAAGAATGCGGCGTGGATAGCGTTTTAATAGCGGATATGCCCCTAATAGAAA
AAGAATTAGTCATCAAATCCGCTCAAAAACACCAAATCAAGCAGATCTTTATCGCCAGCCCTAATGCGAGCGTTAAAGAT
TTAGAACAAGTCGCTACGCATTCGCAAGGCTATATCTACACTTTAGCCAGGAGTGGGGTTACAGGGGCGAGCCATACTTT
AAAAAATGACGCAAGCGCTATTATTAAAACCTTAAAAACCTTTAGCTCTACCCCAGCATTACTAGGCTTTGGCATTTCCA
AAAAAGAACACATCACAAACGCTAAAGGCATGGGTGCTGATGGCGTGATTTGTGGCTCAGCGTTAGTCAAAATCATAGAA
GAAAATTTAAACAATGAAAACGCCATGCTAGAAAAAATTAAAGGGTTTATAGGAGGAATGATTTTTTAA

Upstream 100 bases:

>100_bases
CTCAAAAATGCGAAGAAGAAAGTATCATTGTGGTGAATTTGAGCGGTCGGGGGGATAAGGATTTAAGCACCGTTTATAAC
GCTTTAAAAGGAGGTTTAAA

Downstream 100 bases:

>100_bases
AGGCTTTTAGGCTTTGTTGCGTCAAAAAATTAAAGATCACAGATTAACAATCATCATCAGGTTTTATTGCATGTGCGTTA
TTATGGCGTCAGCATAGAAA

Product: tryptophan synthase subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 262; Mature: 262

Protein sequence:

>262_residues
MRYQNMFETLKKQDKMAFIPFVTLGDPNYEWSFEIIKTLIDSGVSALELGFAFSDPVADGVTIQASHLRALKHASMAKNF
QLLRALRDYNPHIPIGLLAYANLIFSYGVDGFYAQIKECGVDSVLIADMPLIEKELVIKSAQKHQIKQIFIASPNASVKD
LEQVATHSQGYIYTLARSGVTGASHTLKNDASAIIKTLKTFSSTPALLGFGISKKEHITNAKGMGADGVICGSALVKIIE
ENLNNENAMLEKIKGFIGGMIF

Sequences:

>Translated_262_residues
MRYQNMFETLKKQDKMAFIPFVTLGDPNYEWSFEIIKTLIDSGVSALELGFAFSDPVADGVTIQASHLRALKHASMAKNF
QLLRALRDYNPHIPIGLLAYANLIFSYGVDGFYAQIKECGVDSVLIADMPLIEKELVIKSAQKHQIKQIFIASPNASVKD
LEQVATHSQGYIYTLARSGVTGASHTLKNDASAIIKTLKTFSSTPALLGFGISKKEHITNAKGMGADGVICGSALVKIIE
ENLNNENAMLEKIKGFIGGMIF
>Mature_262_residues
MRYQNMFETLKKQDKMAFIPFVTLGDPNYEWSFEIIKTLIDSGVSALELGFAFSDPVADGVTIQASHLRALKHASMAKNF
QLLRALRDYNPHIPIGLLAYANLIFSYGVDGFYAQIKECGVDSVLIADMPLIEKELVIKSAQKHQIKQIFIASPNASVKD
LEQVATHSQGYIYTLARSGVTGASHTLKNDASAIIKTLKTFSSTPALLGFGISKKEHITNAKGMGADGVICGSALVKIIE
ENLNNENAMLEKIKGFIGGMIF

Specific function: The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3- phosphate [H]

COG id: COG0159

COG function: function code E; Tryptophan synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpA family [H]

Homologues:

Organism=Escherichia coli, GI1787514, Length=260, Percent_Identity=46.9230769230769, Blast_Score=233, Evalue=1e-62,
Organism=Saccharomyces cerevisiae, GI6321412, Length=237, Percent_Identity=30.379746835443, Blast_Score=128, Evalue=7e-31,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR011060
- InterPro:   IPR018204
- InterPro:   IPR002028 [H]

Pfam domain/function: PF00290 Trp_syntA [H]

EC number: =4.2.1.20 [H]

Molecular weight: Translated: 28663; Mature: 28663

Theoretical pI: Translated: 8.22; Mature: 8.22

Prosite motif: PS00167 TRP_SYNTHASE_ALPHA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRYQNMFETLKKQDKMAFIPFVTLGDPNYEWSFEIIKTLIDSGVSALELGFAFSDPVADG
CCHHHHHHHHHHCCCEEEEEEEEECCCCCCHHHHHHHHHHHCCCHHHHHCCCCCCCCCCC
VTIQASHLRALKHASMAKNFQLLRALRDYNPHIPIGLLAYANLIFSYGVDGFYAQIKECG
EEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHCC
VDSVLIADMPLIEKELVIKSAQKHQIKQIFIASPNASVKDLEQVATHSQGYIYTLARSGV
CCEEEECCCCHHHHHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHCCCCEEEEEECCCC
TGASHTLKNDASAIIKTLKTFSSTPALLGFGISKKEHITNAKGMGADGVICGSALVKIIE
CCCHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCHHHHCCCCCCCCCCHHHHHHHHHHHH
ENLNNENAMLEKIKGFIGGMIF
HHCCCHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MRYQNMFETLKKQDKMAFIPFVTLGDPNYEWSFEIIKTLIDSGVSALELGFAFSDPVADG
CCHHHHHHHHHHCCCEEEEEEEEECCCCCCHHHHHHHHHHHCCCHHHHHCCCCCCCCCCC
VTIQASHLRALKHASMAKNFQLLRALRDYNPHIPIGLLAYANLIFSYGVDGFYAQIKECG
EEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHCC
VDSVLIADMPLIEKELVIKSAQKHQIKQIFIASPNASVKDLEQVATHSQGYIYTLARSGV
CCEEEECCCCHHHHHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHCCCCEEEEEECCCC
TGASHTLKNDASAIIKTLKTFSSTPALLGFGISKKEHITNAKGMGADGVICGSALVKIIE
CCCHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCHHHHCCCCCCCCCCHHHHHHHHHHHH
ENLNNENAMLEKIKGFIGGMIF
HHCCCHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA