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The map label for this gene is gatA [H]

Identifier: 108563241

GI number: 108563241

Start: 850259

End: 851620

Strand: Reverse

Name: gatA [H]

Synonym: HPAG1_0816

Alternate gene names: 108563241

Gene position: 851620-850259 (Counterclockwise)

Preceding gene: 108563242

Following gene: 108563240

Centisome position: 53.35

GC content: 43.32

Gene sequence:

>1362_bases
ATGATCACTTTAAAACAAGCCCTTTCTTTATCCCAAGATGAATTAGAAACCCTTAAAAACGAAATTGATGCTAAGGTTAG
AGCTTCAGATTTGAACGCTTATATTAAAGCCCCTAGCCTTAATGGCGCTAGCGCTAAAGGCGTGCCGATCCTTATTAAAG
ACAATATCAGCGTTAAGGGGTGGGAAGTTACTTGCTCCAGTAAGATTTTAGAAGGCTATGTCGCTCCTTATCATGCGAGC
GTGATAGAAAACTTGCACCAAAACAGCATGGCAGGGTTTGGGCTTTCTAACATGGACGAGTTTGCGATGGGAAGCACCAC
AGAGTCTAGTTGCTATGGGATCACTAAAAACCCACGAGACAAAAACAGAGTGCCTGGAGGGAGTAGTGGAGGAAGTGCAG
CAGCAGTCGCTGGCGGCTTAGCGGTGGCGGCTTTAGGGAGCGATACGGGCGGGTCTATCAGGCAGCCGGCGAGCTATTGC
GGGTGCGTGGGGTTAAAGCCCACTTATGGGAGGGTGAGCCGTTATGGTTTGATCGCGTATTGCTCTAGTTTTGATCAAAT
CGGGCCTATCACGCAAAATGTAGAAGACGCTTCTATTTTATTTGACGCCATTAGCGGGCATGATAATAAGGACTCCACGA
GCGCTAATCTCAAGCCCACGCAAACCTTTAAAAACCTTAACAGAGACAAACGCTTTAAAATCGCTATTTTGAGAGATCAC
ATTAAAGACGCGAGCAATGAAGTGCAGCTCGCTTATGAAAACACCCTTAAAGCCTTGAAAGAAATGGGGCATGAGATTGT
GGAAAAAAAGATGTTGGATTCGCATTATCAAATCTCTATTTATTATATTATCAGCATGGCTGAAGCGAGTTCGAATCTGG
CCAGATTTGATGGGGTGCGTTATGGGAGGAGGGCTCAAAATATTAAAGATTTGAAAGAATTGTATCTCAAAAGCCGCAGT
GAAGGTTTTGGCGATGAGGTGAAACGGCGCATCATGTTAGGGAATTTTGTCTTAAGCAGTGGGTATTATGACGCTTATTA
TTTGAAAGCCCAGCAAATGCGTTTGATAATTAAAGAGCAATACAACAAGATTTTTGAAGAAGTGGATTTGATTTTCACCC
CTGTTGCTCCTACGAGCGCCCATTTATTCAATTACCATGCAAGCCCTTTAGAAATGTATTTGAGCGATATTTACACGATT
GGGGCGAATTTGAGCGGTTTGCCAGCCCTTTCTTTACCGGTCGCTAAAGATCCTTTAGGCTTGCCCATAGGGATGCAATT
CATTGCTAAGGCTTTTGATGAGCAAAGCCTTTTAGATGTTTCTTACGCTTTAGAGCAAGAATTAGATTTAAAATTAGATT
AA

Upstream 100 bases:

>100_bases
TGAATAAGCAGGTTGAACGCTTTTTAAAAACGCTCTTATAAGCTCGTTTTATCTCTAAGCATGTTAAACTACCATTAAAT
TTTTTAATAAGGCTAAAAAC

Downstream 100 bases:

>100_bases
GGATAGAAAATGAGAATTTTACAAAGGGCTTTGACTTTTGAAGACGTGTTGATGGTGCCTAGAAAGTCCAGCGTTTTACC
TAAAGATGTGAGCTTGAAGT

Product: aspartyl/glutamyl-tRNA amidotransferase subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 453; Mature: 453

Protein sequence:

>453_residues
MITLKQALSLSQDELETLKNEIDAKVRASDLNAYIKAPSLNGASAKGVPILIKDNISVKGWEVTCSSKILEGYVAPYHAS
VIENLHQNSMAGFGLSNMDEFAMGSTTESSCYGITKNPRDKNRVPGGSSGGSAAAVAGGLAVAALGSDTGGSIRQPASYC
GCVGLKPTYGRVSRYGLIAYCSSFDQIGPITQNVEDASILFDAISGHDNKDSTSANLKPTQTFKNLNRDKRFKIAILRDH
IKDASNEVQLAYENTLKALKEMGHEIVEKKMLDSHYQISIYYIISMAEASSNLARFDGVRYGRRAQNIKDLKELYLKSRS
EGFGDEVKRRIMLGNFVLSSGYYDAYYLKAQQMRLIIKEQYNKIFEEVDLIFTPVAPTSAHLFNYHASPLEMYLSDIYTI
GANLSGLPALSLPVAKDPLGLPIGMQFIAKAFDEQSLLDVSYALEQELDLKLD

Sequences:

>Translated_453_residues
MITLKQALSLSQDELETLKNEIDAKVRASDLNAYIKAPSLNGASAKGVPILIKDNISVKGWEVTCSSKILEGYVAPYHAS
VIENLHQNSMAGFGLSNMDEFAMGSTTESSCYGITKNPRDKNRVPGGSSGGSAAAVAGGLAVAALGSDTGGSIRQPASYC
GCVGLKPTYGRVSRYGLIAYCSSFDQIGPITQNVEDASILFDAISGHDNKDSTSANLKPTQTFKNLNRDKRFKIAILRDH
IKDASNEVQLAYENTLKALKEMGHEIVEKKMLDSHYQISIYYIISMAEASSNLARFDGVRYGRRAQNIKDLKELYLKSRS
EGFGDEVKRRIMLGNFVLSSGYYDAYYLKAQQMRLIIKEQYNKIFEEVDLIFTPVAPTSAHLFNYHASPLEMYLSDIYTI
GANLSGLPALSLPVAKDPLGLPIGMQFIAKAFDEQSLLDVSYALEQELDLKLD
>Mature_453_residues
MITLKQALSLSQDELETLKNEIDAKVRASDLNAYIKAPSLNGASAKGVPILIKDNISVKGWEVTCSSKILEGYVAPYHAS
VIENLHQNSMAGFGLSNMDEFAMGSTTESSCYGITKNPRDKNRVPGGSSGGSAAAVAGGLAVAALGSDTGGSIRQPASYC
GCVGLKPTYGRVSRYGLIAYCSSFDQIGPITQNVEDASILFDAISGHDNKDSTSANLKPTQTFKNLNRDKRFKIAILRDH
IKDASNEVQLAYENTLKALKEMGHEIVEKKMLDSHYQISIYYIISMAEASSNLARFDGVRYGRRAQNIKDLKELYLKSRS
EGFGDEVKRRIMLGNFVLSSGYYDAYYLKAQQMRLIIKEQYNKIFEEVDLIFTPVAPTSAHLFNYHASPLEMYLSDIYTI
GANLSGLPALSLPVAKDPLGLPIGMQFIAKAFDEQSLLDVSYALEQELDLKLD

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=463, Percent_Identity=35.6371490280778, Blast_Score=272, Evalue=6e-73,
Organism=Homo sapiens, GI195972892, Length=407, Percent_Identity=23.8329238329238, Blast_Score=99, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17543272, Length=407, Percent_Identity=35.1351351351351, Blast_Score=235, Evalue=3e-62,
Organism=Caenorhabditis elegans, GI17556264, Length=238, Percent_Identity=32.7731092436975, Blast_Score=113, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17537465, Length=445, Percent_Identity=25.6179775280899, Blast_Score=110, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI71990152, Length=264, Percent_Identity=31.0606060606061, Blast_Score=104, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17556276, Length=249, Percent_Identity=30.9236947791165, Blast_Score=92, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI17556278, Length=249, Percent_Identity=30.9236947791165, Blast_Score=92, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI17538252, Length=259, Percent_Identity=24.7104247104247, Blast_Score=84, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6323950, Length=417, Percent_Identity=35.4916067146283, Blast_Score=224, Evalue=2e-59,
Organism=Saccharomyces cerevisiae, GI6319685, Length=396, Percent_Identity=27.5252525252525, Blast_Score=99, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24648113, Length=465, Percent_Identity=37.6344086021505, Blast_Score=270, Evalue=1e-72,
Organism=Drosophila melanogaster, GI24644968, Length=428, Percent_Identity=27.3364485981308, Blast_Score=118, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24652985, Length=418, Percent_Identity=27.2727272727273, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI19922090, Length=418, Percent_Identity=27.2727272727273, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24652981, Length=418, Percent_Identity=27.2727272727273, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24652983, Length=418, Percent_Identity=27.2727272727273, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI45550774, Length=433, Percent_Identity=25.1732101616628, Blast_Score=93, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24648435, Length=433, Percent_Identity=25.1732101616628, Blast_Score=93, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24648437, Length=433, Percent_Identity=25.1732101616628, Blast_Score=93, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24648441, Length=443, Percent_Identity=24.8306997742664, Blast_Score=92, Evalue=7e-19,
Organism=Drosophila melanogaster, GI24648439, Length=443, Percent_Identity=24.8306997742664, Blast_Score=92, Evalue=7e-19,
Organism=Drosophila melanogaster, GI161078093, Length=418, Percent_Identity=25.5980861244019, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI21356731, Length=130, Percent_Identity=35.3846153846154, Blast_Score=70, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.-

Molecular weight: Translated: 49661; Mature: 49661

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITLKQALSLSQDELETLKNEIDAKVRASDLNAYIKAPSLNGASAKGVPILIKDNISVKG
CCCHHHHHCCCHHHHHHHHHHHCCEEEECCCCEEEECCCCCCCCCCCCEEEEECCCEEEE
WEVTCSSKILEGYVAPYHASVIENLHQNSMAGFGLSNMDEFAMGSTTESSCYGITKNPRD
EEEEHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCC
KNRVPGGSSGGSAAAVAGGLAVAALGSDTGGSIRQPASYCGCVGLKPTYGRVSRYGLIAY
CCCCCCCCCCCCHHHHHCCEEEEEECCCCCCCCCCCHHHHCCCCCCCCHHHHHHHHHHHH
CSSFDQIGPITQNVEDASILFDAISGHDNKDSTSANLKPTQTFKNLNRDKRFKIAILRDH
HHCHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHCCCCCEEEEEEEHHH
IKDASNEVQLAYENTLKALKEMGHEIVEKKMLDSHYQISIYYIISMAEASSNLARFDGVR
HCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEEEHHHHCCHHHHCCCH
YGRRAQNIKDLKELYLKSRSEGFGDEVKRRIMLGNFVLSSGYYDAYYLKAQQMRLIIKEQ
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHH
YNKIFEEVDLIFTPVAPTSAHLFNYHASPLEMYLSDIYTIGANLSGLPALSLPVAKDPLG
HHHHHHHHHEEEECCCCCCHHHEECCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
LPIGMQFIAKAFDEQSLLDVSYALEQELDLKLD
CCHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MITLKQALSLSQDELETLKNEIDAKVRASDLNAYIKAPSLNGASAKGVPILIKDNISVKG
CCCHHHHHCCCHHHHHHHHHHHCCEEEECCCCEEEECCCCCCCCCCCCEEEEECCCEEEE
WEVTCSSKILEGYVAPYHASVIENLHQNSMAGFGLSNMDEFAMGSTTESSCYGITKNPRD
EEEEHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCC
KNRVPGGSSGGSAAAVAGGLAVAALGSDTGGSIRQPASYCGCVGLKPTYGRVSRYGLIAY
CCCCCCCCCCCCHHHHHCCEEEEEECCCCCCCCCCCHHHHCCCCCCCCHHHHHHHHHHHH
CSSFDQIGPITQNVEDASILFDAISGHDNKDSTSANLKPTQTFKNLNRDKRFKIAILRDH
HHCHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHCCCCCEEEEEEEHHH
IKDASNEVQLAYENTLKALKEMGHEIVEKKMLDSHYQISIYYIISMAEASSNLARFDGVR
HCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEEEHHHHCCHHHHCCCH
YGRRAQNIKDLKELYLKSRSEGFGDEVKRRIMLGNFVLSSGYYDAYYLKAQQMRLIIKEQ
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHH
YNKIFEEVDLIFTPVAPTSAHLFNYHASPLEMYLSDIYTIGANLSGLPALSLPVAKDPLG
HHHHHHHHHEEEECCCCCCHHHEECCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
LPIGMQFIAKAFDEQSLLDVSYALEQELDLKLD
CCHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA