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The map label for this gene is trmB

Identifier: 108563157

GI number: 108563157

Start: 769775

End: 770956

Strand: Direct

Name: trmB

Synonym: HPAG1_0732

Alternate gene names: 108563157

Gene position: 769775-770956 (Clockwise)

Preceding gene: 108563156

Following gene: 108563158

Centisome position: 48.22

GC content: 34.69

Gene sequence:

>1182_bases
ATGCCCCATTTTTTAGCCAAGCTGGATTTTAAACCTTTAAAATACCCCTTAATTGAAGGGGATTTTTGTTTTCATAAGGA
ATTTTTAAGCCTAAAAAACCCCACTAAAAGCTGTGTGTATGCGAGTTTTAAGGATCGCATTTTTTTATTGCAAAAAATCA
GGCGAGCGAATGATTTTTTAATCAAAAGCGAAAAAACAACGCCCTTAAAACGAGAAATTTTAAAACAAGCTTTAAGGATT
TATTCGCAATCTTTTGAAGTTATTTCGCATAATTTGCAAGAAAATTCTAAACATGCGAGCGGAAAAAAAGCCCTTGATTT
AGGAACTTTTGAAGACTTTATTCAAAAAAATCAAGCCCCTATTTTAGCCGAAATTGGTTTTGGGAGCGGGAGGCATTTGA
TAGAATTAGCCAAAAACAACCCCACTAAAACATGCTTAGGGATAGAGATTCACACCCCCTCTATCGCGCAAGCGTTAAAG
CAAATTGAATTGTTGGATTTAAAAAATCTGCACATCTTGCAAGGCGATGGCCGTTTGATTTTAGAGAGCATGCCAAATTA
TAAGTGCGAGAAAATTTTTGTGCATTTCCCTGTGCCATGGAATGAGAAAAAACACCGCCGTGTGTTAAGCGAAAAATTTT
TAAACGAAGCTTTAAGGGTTTTAAAGCCTAGAGGGTTTTTGGAATTACGAACCGATGATGGTCTTTATTTTGAAGACAGC
CTAAAATTAGCCCTAAAAAACTTTCAATGCGAGATAGAAATCAAAAAAAACGCTCAAATACCGGTTATCAGCAAGTATGA
AGCGCGTTGGAATAAACTCAAAAAAGATATTTATGATTTAAGAATTTATTCTTTAGAATTAAATGAAACCCCTTTTTACA
ACCATGCATTTGATTTTTCTTTTGATACAATAACAATGAGTGAAAAGAGCGTTGGAACGATTTTAAAAACCAAAAAAATC
ATCCAAGAGGGGTATTTTGTGCATGTTTGTAATATTTATGAGAATAAGGGGGATTTTTTAGTGGAATTGAGCATGGGGGA
TTTTGATTGGCCTGTGCGTTTGTTTGTTTTACTGACAGAAAACCAGATTTTTTACCTCAATAAAAGCCCCTTAAAAACCT
TAAACAACCACAAAGCGCATCTTTTGCTTCAAAATATCCTAAGCCAAAAGGGAATTGGATGA

Upstream 100 bases:

>100_bases
GTGGCTTATCGCTATCAAGTGGTGAGCGTGGATAAGGATGGATTAGAGTCGCACCCAAGCAAAGAAGTGCGTTTGTTTTT
AGAGCGCTAAAAGGGTTTTA

Downstream 100 bases:

>100_bases
GTGTGATTATCGCAGCGAATAATTTATGCTTACAATACCAGCAAAACGAACCGGTCATTAAGCATGCCAATTTACGCATC
AAACGCAAGGATTTTGTGTT

Product: tRNA (guanine-N(7)-)-methyltransferase

Products: NA

Alternate protein names: tRNA(m7G46)-methyltransferase

Number of amino acids: Translated: 393; Mature: 392

Protein sequence:

>393_residues
MPHFLAKLDFKPLKYPLIEGDFCFHKEFLSLKNPTKSCVYASFKDRIFLLQKIRRANDFLIKSEKTTPLKREILKQALRI
YSQSFEVISHNLQENSKHASGKKALDLGTFEDFIQKNQAPILAEIGFGSGRHLIELAKNNPTKTCLGIEIHTPSIAQALK
QIELLDLKNLHILQGDGRLILESMPNYKCEKIFVHFPVPWNEKKHRRVLSEKFLNEALRVLKPRGFLELRTDDGLYFEDS
LKLALKNFQCEIEIKKNAQIPVISKYEARWNKLKKDIYDLRIYSLELNETPFYNHAFDFSFDTITMSEKSVGTILKTKKI
IQEGYFVHVCNIYENKGDFLVELSMGDFDWPVRLFVLLTENQIFYLNKSPLKTLNNHKAHLLLQNILSQKGIG

Sequences:

>Translated_393_residues
MPHFLAKLDFKPLKYPLIEGDFCFHKEFLSLKNPTKSCVYASFKDRIFLLQKIRRANDFLIKSEKTTPLKREILKQALRI
YSQSFEVISHNLQENSKHASGKKALDLGTFEDFIQKNQAPILAEIGFGSGRHLIELAKNNPTKTCLGIEIHTPSIAQALK
QIELLDLKNLHILQGDGRLILESMPNYKCEKIFVHFPVPWNEKKHRRVLSEKFLNEALRVLKPRGFLELRTDDGLYFEDS
LKLALKNFQCEIEIKKNAQIPVISKYEARWNKLKKDIYDLRIYSLELNETPFYNHAFDFSFDTITMSEKSVGTILKTKKI
IQEGYFVHVCNIYENKGDFLVELSMGDFDWPVRLFVLLTENQIFYLNKSPLKTLNNHKAHLLLQNILSQKGIG
>Mature_392_residues
PHFLAKLDFKPLKYPLIEGDFCFHKEFLSLKNPTKSCVYASFKDRIFLLQKIRRANDFLIKSEKTTPLKREILKQALRIY
SQSFEVISHNLQENSKHASGKKALDLGTFEDFIQKNQAPILAEIGFGSGRHLIELAKNNPTKTCLGIEIHTPSIAQALKQ
IELLDLKNLHILQGDGRLILESMPNYKCEKIFVHFPVPWNEKKHRRVLSEKFLNEALRVLKPRGFLELRTDDGLYFEDSL
KLALKNFQCEIEIKKNAQIPVISKYEARWNKLKKDIYDLRIYSLELNETPFYNHAFDFSFDTITMSEKSVGTILKTKKII
QEGYFVHVCNIYENKGDFLVELSMGDFDWPVRLFVLLTENQIFYLNKSPLKTLNNHKAHLLLQNILSQKGIG

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA

COG id: COG0220

COG function: function code R; Predicted S-adenosylmethionine-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. TrmB family

Homologues:

Organism=Escherichia coli, GI1789330, Length=172, Percent_Identity=30.2325581395349, Blast_Score=80, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMB_HELPH (Q1CTC3)

Other databases:

- EMBL:   CP000241
- RefSeq:   YP_627473.1
- ProteinModelPortal:   Q1CTC3
- SMR:   Q1CTC3
- STRING:   Q1CTC3
- GeneID:   4098820
- GenomeReviews:   CP000241_GR
- KEGG:   hpa:HPAG1_0732
- eggNOG:   COG0220
- HOGENOM:   HBG321449
- OMA:   FVHFPVP
- ProtClustDB:   PRK14121
- BioCyc:   HPYL357544:HPAG1_0732-MONOMER
- HAMAP:   MF_01057
- InterPro:   IPR003358
- PANTHER:   PTHR23417:SF1
- TIGRFAMs:   TIGR00091

Pfam domain/function: PF02390 Methyltransf_4

EC number: =2.1.1.33

Molecular weight: Translated: 45708; Mature: 45577

Theoretical pI: Translated: 9.61; Mature: 9.61

Prosite motif: NA

Important sites: BINDING 124-124 BINDING 149-149 BINDING 176-176 BINDING 232-232

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPHFLAKLDFKPLKYPLIEGDFCFHKEFLSLKNPTKSCVYASFKDRIFLLQKIRRANDFL
CCCCHHCCCCCCCCCCEECCCHHHHHHHHHCCCCCCHHEEHHHHHHHHHHHHHHCCCCEE
IKSEKTTPLKREILKQALRIYSQSFEVISHNLQENSKHASGKKALDLGTFEDFIQKNQAP
EECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCHHHHHHHCCCCC
ILAEIGFGSGRHLIELAKNNPTKTCLGIEIHTPSIAQALKQIELLDLKNLHILQGDGRLI
EEEEECCCCCCEEEEEECCCCCCEEEEEEEECCHHHHHHHHHHHHCCCCEEEEECCCEEE
LESMPNYKCEKIFVHFPVPWNEKKHRRVLSEKFLNEALRVLKPRGFLELRTDDGLYFEDS
EECCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEHHH
LKLALKNFQCEIEIKKNAQIPVISKYEARWNKLKKDIYDLRIYSLELNETPFYNHAFDFS
HHHEEECCEEEEEEECCCCCCEEEHHHHHHHHHHHHHHEEEEEEEEECCCCCCCCEEECE
FDTITMSEKSVGTILKTKKIIQEGYFVHVCNIYENKGDFLVELSMGDFDWPVRLFVLLTE
EEEEEECCHHHHHHHHHHHHHHCCCEEEEEEEEECCCCEEEEEECCCCCCCEEEEEEEEC
NQIFYLNKSPLKTLNNHKAHLLLQNILSQKGIG
CEEEEECCCHHHHHCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
PHFLAKLDFKPLKYPLIEGDFCFHKEFLSLKNPTKSCVYASFKDRIFLLQKIRRANDFL
CCCHHCCCCCCCCCCEECCCHHHHHHHHHCCCCCCHHEEHHHHHHHHHHHHHHCCCCEE
IKSEKTTPLKREILKQALRIYSQSFEVISHNLQENSKHASGKKALDLGTFEDFIQKNQAP
EECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCHHHHHHHCCCCC
ILAEIGFGSGRHLIELAKNNPTKTCLGIEIHTPSIAQALKQIELLDLKNLHILQGDGRLI
EEEEECCCCCCEEEEEECCCCCCEEEEEEEECCHHHHHHHHHHHHCCCCEEEEECCCEEE
LESMPNYKCEKIFVHFPVPWNEKKHRRVLSEKFLNEALRVLKPRGFLELRTDDGLYFEDS
EECCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEHHH
LKLALKNFQCEIEIKKNAQIPVISKYEARWNKLKKDIYDLRIYSLELNETPFYNHAFDFS
HHHEEECCEEEEEEECCCCCCEEEHHHHHHHHHHHHHHEEEEEEEEECCCCCCCCEEECE
FDTITMSEKSVGTILKTKKIIQEGYFVHVCNIYENKGDFLVELSMGDFDWPVRLFVLLTE
EEEEEECCHHHHHHHHHHHHHHCCCEEEEEEEEECCCCEEEEEECCCCCCCEEEEEEEEC
NQIFYLNKSPLKTLNNHKAHLLLQNILSQKGIG
CEEEEECCCHHHHHCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA