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The map label for this gene is nth [H]

Identifier: 108562989

GI number: 108562989

Start: 581032

End: 581595

Strand: Reverse

Name: nth [H]

Synonym: HPAG1_0564

Alternate gene names: 108562989

Gene position: 581595-581032 (Counterclockwise)

Preceding gene: 108562990

Following gene: 108562988

Centisome position: 36.43

GC content: 41.31

Gene sequence:

>564_bases
TTGCACCATAAAAACCCCTATGAGTTATTGGTGGCTACCATTTTAAGTGCCCAATGTACGGACGCTAGAGTGAATCAAAT
AACGCCCAAGCTGTTTGAAAAATACCCCAGCGTGAACGATTTAGCGCTCGCTTCTTTAGAAGAGGTTAAAGAGATCATTA
AATCCGTTTCGTATTCCAACAATAAAAGCAAGCATTTAATTAATATGGCACAAAAAGTGGTTAGGGATTTTAAGGGCGTT
ATCCCCTCTACGCAAAAAGAATTGATGAGTTTAGATGGCGTGGGGCAAAAAACCGCTAATGTGGTGCTTTCAGTGTGCTT
TGATGCAAATTATATGGCGGTAGATACCCATGTGTTCCGCACGACACACCGATTAGGTTTAAGTAGTGCTAACACGCCCA
CTAAGACCGAAGAAGAACTCAGCGATCTTTTTAAAGACAACCTGTCCAAACTCCACCATGCCTTAATCTTGTTTGGCCGT
TATACCTGCAAAGCTAAAAACCCCTTATGCGATGCGTGTTTTTTAAAAGAATTTTGCGTTTCTAAAGCTAGCTTTAAGGC
GTAG

Upstream 100 bases:

>100_bases
GAAAGCAAGCAAGAATGAGCTTAAAACGCACTAAAACTTACCAAAAAGCCCAACAAATCAAAGAGTTGCTCTTAAAACAT
TACCCCACCAAACCACCGAG

Downstream 100 bases:

>100_bases
TGGCTTTATTTGTTATAAAATTTATAGTAAAATTAAGATTTTATAATCAAAGTCAAATCATTCTTGAAAGAGTAGAGTTT
TATGCTAGAAACAGAATCTA

Product: endonuclease III

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase [H]

Number of amino acids: Translated: 187; Mature: 187

Protein sequence:

>187_residues
MHHKNPYELLVATILSAQCTDARVNQITPKLFEKYPSVNDLALASLEEVKEIIKSVSYSNNKSKHLINMAQKVVRDFKGV
IPSTQKELMSLDGVGQKTANVVLSVCFDANYMAVDTHVFRTTHRLGLSSANTPTKTEEELSDLFKDNLSKLHHALILFGR
YTCKAKNPLCDACFLKEFCVSKASFKA

Sequences:

>Translated_187_residues
MHHKNPYELLVATILSAQCTDARVNQITPKLFEKYPSVNDLALASLEEVKEIIKSVSYSNNKSKHLINMAQKVVRDFKGV
IPSTQKELMSLDGVGQKTANVVLSVCFDANYMAVDTHVFRTTHRLGLSSANTPTKTEEELSDLFKDNLSKLHHALILFGR
YTCKAKNPLCDACFLKEFCVSKASFKA
>Mature_187_residues
MHHKNPYELLVATILSAQCTDARVNQITPKLFEKYPSVNDLALASLEEVKEIIKSVSYSNNKSKHLINMAQKVVRDFKGV
IPSTQKELMSLDGVGQKTANVVLSVCFDANYMAVDTHVFRTTHRLGLSSANTPTKTEEELSDLFKDNLSKLHHALILFGR
YTCKAKNPLCDACFLKEFCVSKASFKA

Specific function: Has Both An Apurinic And/Or Apyrimidinic Endonuclease Activity And A DNA N-Glycosylase Activity. Incises Damaged DNA At Cytosines, Thymines And Guanines. Acts On A Damaged Strand, 5' From The Damaged Site. Required For The Repair Of Both Oxidative DNA Da

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI190358497, Length=186, Percent_Identity=26.8817204301075, Blast_Score=78, Evalue=4e-15,
Organism=Homo sapiens, GI6912520, Length=186, Percent_Identity=26.8817204301075, Blast_Score=78, Evalue=4e-15,
Organism=Homo sapiens, GI115298650, Length=187, Percent_Identity=27.807486631016, Blast_Score=78, Evalue=4e-15,
Organism=Homo sapiens, GI115298648, Length=186, Percent_Identity=26.8817204301075, Blast_Score=78, Evalue=4e-15,
Organism=Homo sapiens, GI115298654, Length=187, Percent_Identity=27.807486631016, Blast_Score=78, Evalue=5e-15,
Organism=Homo sapiens, GI115298652, Length=187, Percent_Identity=27.807486631016, Blast_Score=78, Evalue=5e-15,
Organism=Homo sapiens, GI4505471, Length=177, Percent_Identity=26.5536723163842, Blast_Score=76, Evalue=1e-14,
Organism=Escherichia coli, GI1787920, Length=183, Percent_Identity=37.7049180327869, Blast_Score=127, Evalue=5e-31,
Organism=Escherichia coli, GI1789331, Length=187, Percent_Identity=26.2032085561497, Blast_Score=76, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17554540, Length=177, Percent_Identity=31.0734463276836, Blast_Score=91, Evalue=4e-19,
Organism=Drosophila melanogaster, GI45550361, Length=179, Percent_Identity=27.9329608938547, Blast_Score=77, Evalue=9e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF00633 HHH; PF00730 HhH-GPD [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 20897; Mature: 20897

Theoretical pI: Translated: 8.80; Mature: 8.80

Prosite motif: PS00764 ENDONUCLEASE_III_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHHKNPYELLVATILSAQCTDARVNQITPKLFEKYPSVNDLALASLEEVKEIIKSVSYSN
CCCCCHHHHHHHHHHHHHCCHHHHHHCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCC
NKSKHLINMAQKVVRDFKGVIPSTQKELMSLDGVGQKTANVVLSVCFDANYMAVDTHVFR
CCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCHHHHHHHHHHHHCCCEEEHHHHHHH
TTHRLGLSSANTPTKTEEELSDLFKDNLSKLHHALILFGRYTCKAKNPLCDACFLKEFCV
HHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
SKASFKA
HHHHCCC
>Mature Secondary Structure
MHHKNPYELLVATILSAQCTDARVNQITPKLFEKYPSVNDLALASLEEVKEIIKSVSYSN
CCCCCHHHHHHHHHHHHHCCHHHHHHCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCC
NKSKHLINMAQKVVRDFKGVIPSTQKELMSLDGVGQKTANVVLSVCFDANYMAVDTHVFR
CCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCHHHHHHHHHHHHCCCEEEHHHHHHH
TTHRLGLSSANTPTKTEEELSDLFKDNLSKLHHALILFGRYTCKAKNPLCDACFLKEFCV
HHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
SKASFKA
HHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7704260; 8760912; 9384377 [H]