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The map label for this gene is hsdM [H]

Identifier: 108562863

GI number: 108562863

Start: 448537

End: 450168

Strand: Reverse

Name: hsdM [H]

Synonym: HPAG1_0438

Alternate gene names: 108562863

Gene position: 450168-448537 (Counterclockwise)

Preceding gene: 108562864

Following gene: 108562862

Centisome position: 28.2

GC content: 37.32

Gene sequence:

>1632_bases
ATGCCTAATAACGCTTTATTGCAAATCAAACAAGACACCCTAAGCCTCATTGATGATTTAAAAGTCATTTGCGCAAGTTT
TGGTTTAGGGAATGACGGCAACGAATACAAAATCATCACACAATGCTTTTTGTATAAATTCTTATGCGATAAGTTTGAAT
TCCTTTTTGAACAAGAATTCCCTAACCAAACGATACAAGATTACAAAGACTTTAACAAGGAAGAAAAAGAAGATTTCTTT
ATTACCTTAACCGATAAAAGACTCCCCAAACTCGCCTATGATGAGCTTTTAAGCTATCTTTTTGAAAAACATTTTAATGA
TAACGATTTACACCTAAAGCTAGACGCTATTTTTAATCGTATTTCTAGCAATAATGCCGAGCTTTTCAACACCACAAGCA
CGGATAAAACCACTATCGCCTTATTTGAAAGCGTCTCACAATACATTAATGAAGAGTCTAAAAGGGCTAATTTTACAAGA
GTTTTATTAGACAAACTCAAAAAATTTAATTTCAAACAAGCTTTTTTAAACTTACAAAACCAACAAGGCTATGATTTTTT
CGCCCCCATTTTTGAATACTTACTCAAAGATTACAATAACGCCGGCACAGGCAAATACGCCGAATACTACACCCCTTTAA
GCATCGCTAGCATCATTGCTAAGCTTTTAATTAATGAGCCGACTCAAAGCGTCAAAATCTATGACCCAAGCGCTGGCACA
GGAACGCTTTTAATGGCATTAGCCCACCAAATAGGCACCAATTCTTGCACCCTTTATGCCCAAGACATTTCGCAAAAATC
CTTAAGAATGCTCAAACTCAACCTCATTTTAAACGACTTGACCCACTCTTTAAAAAACGCCATTGAGGGAAACACTTTAA
CCAACCCCTACCATTCTAAAGAATGTCATGGGAAAATGGATTTCATCGTGAGTAACCCCCCTTTCAAGCTGGATTTTTCC
AACGAGCATGCCGAGATTTCGCAAAACAAAAACGATTTTTTCTTAGGCGTGCCTAATATCCCTAAAAACGATAAAAGCAA
AATGCCCATTTACACGCTCTTTTTCCAGCATTGCTTGAACATGCTTAGCCATAAAGGTAAGGGGGCTATTATCGTGCCAA
CCGGATTCATTAGTGCTAAAAGCGGGGTAGAAAATAAGATTGTCCGGCATTTAGTGGATGAAAGGCTCGTTTATGGGGTG
GTTTGCATGCCCAGTCAAGTTTTTGCCAACACCGGCACTAATGTGAGCATCATCTTTTTTCAAAAAACGCCAAGCGCAAA
GGAAGTGGTTTTGATTGACGCTTCCAAACTCGGCGAAGAATACACCGAAAACAAAAACAAAAAAACGCGCTTAAGAACAA
GCGATATTGGTTTGATTTTAGAAACTTTTCAAAATAAAACCCAAAAAGCGGATTTTTGCGCTCTGGTTTCTTTTGATGAA
ATTACAGAAAAAAATTATTCTCTAAACCCCGGGCAGTATTTCACTATAGAGGACACAAGCGAGAAAATCAGCCAAGCGGA
GTTTGAAAACCTAATGCAACAATATTCAAGCGAATTAACAAGCCTTTTTGATGAAAGCCAGAGCTTGCAACAAGAAATTT
TAGAAACTTTAGGAAATCTTAATTATGACTAA

Upstream 100 bases:

>100_bases
AAAGAAAACCCCTCCTTAAAAGATTTGGAAAAAGAAAAAGAACTTATCATTCAAACCCTTTTTAACGAACTCACACAAAA
CCACCATCAAGGAAATTCAC

Downstream 100 bases:

>100_bases
ACTTTTAGATTTAAAAAATAATTCAGTAATTACTATCAATCAAAACAACTACACTAAAGAAGACAATTACAAAAAAGTTT
ATTATTTAGATACTGATAAT

Product: type I restriction enzyme M protein

Products: NA

Alternate protein names: M.EcoR124II [H]

Number of amino acids: Translated: 543; Mature: 542

Protein sequence:

>543_residues
MPNNALLQIKQDTLSLIDDLKVICASFGLGNDGNEYKIITQCFLYKFLCDKFEFLFEQEFPNQTIQDYKDFNKEEKEDFF
ITLTDKRLPKLAYDELLSYLFEKHFNDNDLHLKLDAIFNRISSNNAELFNTTSTDKTTIALFESVSQYINEESKRANFTR
VLLDKLKKFNFKQAFLNLQNQQGYDFFAPIFEYLLKDYNNAGTGKYAEYYTPLSIASIIAKLLINEPTQSVKIYDPSAGT
GTLLMALAHQIGTNSCTLYAQDISQKSLRMLKLNLILNDLTHSLKNAIEGNTLTNPYHSKECHGKMDFIVSNPPFKLDFS
NEHAEISQNKNDFFLGVPNIPKNDKSKMPIYTLFFQHCLNMLSHKGKGAIIVPTGFISAKSGVENKIVRHLVDERLVYGV
VCMPSQVFANTGTNVSIIFFQKTPSAKEVVLIDASKLGEEYTENKNKKTRLRTSDIGLILETFQNKTQKADFCALVSFDE
ITEKNYSLNPGQYFTIEDTSEKISQAEFENLMQQYSSELTSLFDESQSLQQEILETLGNLNYD

Sequences:

>Translated_543_residues
MPNNALLQIKQDTLSLIDDLKVICASFGLGNDGNEYKIITQCFLYKFLCDKFEFLFEQEFPNQTIQDYKDFNKEEKEDFF
ITLTDKRLPKLAYDELLSYLFEKHFNDNDLHLKLDAIFNRISSNNAELFNTTSTDKTTIALFESVSQYINEESKRANFTR
VLLDKLKKFNFKQAFLNLQNQQGYDFFAPIFEYLLKDYNNAGTGKYAEYYTPLSIASIIAKLLINEPTQSVKIYDPSAGT
GTLLMALAHQIGTNSCTLYAQDISQKSLRMLKLNLILNDLTHSLKNAIEGNTLTNPYHSKECHGKMDFIVSNPPFKLDFS
NEHAEISQNKNDFFLGVPNIPKNDKSKMPIYTLFFQHCLNMLSHKGKGAIIVPTGFISAKSGVENKIVRHLVDERLVYGV
VCMPSQVFANTGTNVSIIFFQKTPSAKEVVLIDASKLGEEYTENKNKKTRLRTSDIGLILETFQNKTQKADFCALVSFDE
ITEKNYSLNPGQYFTIEDTSEKISQAEFENLMQQYSSELTSLFDESQSLQQEILETLGNLNYD
>Mature_542_residues
PNNALLQIKQDTLSLIDDLKVICASFGLGNDGNEYKIITQCFLYKFLCDKFEFLFEQEFPNQTIQDYKDFNKEEKEDFFI
TLTDKRLPKLAYDELLSYLFEKHFNDNDLHLKLDAIFNRISSNNAELFNTTSTDKTTIALFESVSQYINEESKRANFTRV
LLDKLKKFNFKQAFLNLQNQQGYDFFAPIFEYLLKDYNNAGTGKYAEYYTPLSIASIIAKLLINEPTQSVKIYDPSAGTG
TLLMALAHQIGTNSCTLYAQDISQKSLRMLKLNLILNDLTHSLKNAIEGNTLTNPYHSKECHGKMDFIVSNPPFKLDFSN
EHAEISQNKNDFFLGVPNIPKNDKSKMPIYTLFFQHCLNMLSHKGKGAIIVPTGFISAKSGVENKIVRHLVDERLVYGVV
CMPSQVFANTGTNVSIIFFQKTPSAKEVVLIDASKLGEEYTENKNKKTRLRTSDIGLILETFQNKTQKADFCALVSFDEI
TEKNYSLNPGQYFTIEDTSEKISQAEFENLMQQYSSELTSLFDESQSLQQEILETLGNLNYD

Specific function: Methylation of specific adenine residues; required for both restriction and modification activities. The EcoR124/3 I enzyme recognizes 5'-GAAN(7)RTCG-3' [H]

COG id: COG0286

COG function: function code V; Type I restriction-modification system methyltransferase subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022749
- InterPro:   IPR003356
- InterPro:   IPR002052
- InterPro:   IPR002296
- InterPro:   IPR004546 [H]

Pfam domain/function: PF12161 HsdM_N; PF02384 N6_Mtase [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 62039; Mature: 61908

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPNNALLQIKQDTLSLIDDLKVICASFGLGNDGNEYKIITQCFLYKFLCDKFEFLFEQEF
CCCCCEEEEHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
PNQTIQDYKDFNKEEKEDFFITLTDKRLPKLAYDELLSYLFEKHFNDNDLHLKLDAIFNR
CCCHHHHHHCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHH
ISSNNAELFNTTSTDKTTIALFESVSQYINEESKRANFTRVLLDKLKKFNFKQAFLNLQN
HCCCCCEEEECCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCHHHHHHHCCC
QQGYDFFAPIFEYLLKDYNNAGTGKYAEYYTPLSIASIIAKLLINEPTQSVKIYDPSAGT
CCCCHHHHHHHHHHHHHCCCCCCCCCHHHCCHHHHHHHHHHHHHCCCCCCEEEECCCCCH
GTLLMALAHQIGTNSCTLYAQDISQKSLRMLKLNLILNDLTHSLKNAIEGNTLTNPYHSK
HHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
ECHGKMDFIVSNPPFKLDFSNEHAEISQNKNDFFLGVPNIPKNDKSKMPIYTLFFQHCLN
CCCCCEEEEECCCCEEEECCCCCHHHCCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHH
MLSHKGKGAIIVPTGFISAKSGVENKIVRHLVDERLVYGVVCMPSQVFANTGTNVSIIFF
HHHCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHEEEECCHHHHHCCCCCEEEEEE
QKTPSAKEVVLIDASKLGEEYTENKNKKTRLRTSDIGLILETFQNKTQKADFCALVSFDE
ECCCCCCEEEEEEHHHHCHHHHHCCCCHHEEEHHHHHHHHHHHHCCCCCCCEEEEEEHHH
ITEKNYSLNPGQYFTIEDTSEKISQAEFENLMQQYSSELTSLFDESQSLQQEILETLGNL
HHCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
NYD
CCC
>Mature Secondary Structure 
PNNALLQIKQDTLSLIDDLKVICASFGLGNDGNEYKIITQCFLYKFLCDKFEFLFEQEF
CCCCEEEEHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
PNQTIQDYKDFNKEEKEDFFITLTDKRLPKLAYDELLSYLFEKHFNDNDLHLKLDAIFNR
CCCHHHHHHCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHH
ISSNNAELFNTTSTDKTTIALFESVSQYINEESKRANFTRVLLDKLKKFNFKQAFLNLQN
HCCCCCEEEECCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCHHHHHHHCCC
QQGYDFFAPIFEYLLKDYNNAGTGKYAEYYTPLSIASIIAKLLINEPTQSVKIYDPSAGT
CCCCHHHHHHHHHHHHHCCCCCCCCCHHHCCHHHHHHHHHHHHHCCCCCCEEEECCCCCH
GTLLMALAHQIGTNSCTLYAQDISQKSLRMLKLNLILNDLTHSLKNAIEGNTLTNPYHSK
HHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
ECHGKMDFIVSNPPFKLDFSNEHAEISQNKNDFFLGVPNIPKNDKSKMPIYTLFFQHCLN
CCCCCEEEEECCCCEEEECCCCCHHHCCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHH
MLSHKGKGAIIVPTGFISAKSGVENKIVRHLVDERLVYGVVCMPSQVFANTGTNVSIIFF
HHHCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHEEEECCHHHHHCCCCCEEEEEE
QKTPSAKEVVLIDASKLGEEYTENKNKKTRLRTSDIGLILETFQNKTQKADFCALVSFDE
ECCCCCCEEEEEEHHHHCHHHHHCCCCHHEEEHHHHHHHHHHHHCCCCCCCEEEEEEHHH
ITEKNYSLNPGQYFTIEDTSEKISQAEFENLMQQYSSELTSLFDESQSLQQEILETLGNL
HHCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
NYD
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2784505 [H]