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The map label for this gene is tgt [H]

Identifier: 108562708

GI number: 108562708

Start: 286351

End: 287466

Strand: Reverse

Name: tgt [H]

Synonym: HPAG1_0283

Alternate gene names: 108562708

Gene position: 287466-286351 (Counterclockwise)

Preceding gene: 108562722

Following gene: 108562688

Centisome position: 18.01

GC content: 43.82

Gene sequence:

>1116_bases
ATGGATTTTCAACTCCAAGCTGTTGATGATAACGCGCGAGCTGGTCTTTTAAATTTAGCCCATTCTCAAGTGGCAACGCC
TGTTTTTATGCCAGTAGGCACGCAAGGCTGCATCAAATCTTTAGACGCTACAGATGCGCAAGAAATTTTAGGCGCTAAAC
TCATTTTAGCCAACACCTATCACATGTATTTAAGGCCGGGTGAGAAAGTGGTTGGACAATTAGGGGGCTTGCATCGTTTC
GCTCAATTTCAGGGGAGTTTTTTAACCGATAGCGGAGGGTTTCAAGCCTTCAGTTTGAGCGGCAATATCAAATTGCAAGA
AGACGGAATTGTTTTTAAATCCCATATTGATGGAAGCAAGCATTTATTCACGCCCGCTAAAGTTTTGGACATTCAATATT
CTTTGAATAGCGATATTATGATGGTTTTAGACGATTTAGTGGGCTTGCCCGCCCCCTTAAAACGCCTTGAAGAATCCATT
AAAAGAAGCGCCAAATGGGCGAATCTCAGCCTAGAATACCACAAGCAAAAAAACCGCCCGAGCAACAACCTTTTTGCCAT
TATCCAGGGCGGGACGCATTTGAAAATGCGCAGCCTTAGCGTGGGATTAACGCATGAGGGTTTTGATGGCTATGCTATAG
GCGGTTTAGCGGTGGGGGAAAGCGTGGATGAAATGCTAGAAACCATCGCGCACACCGCCCCCTTGCTCCCCAAAGACAAG
CCTCGCTACTTAATGGGTGTAGGCACGCCTGAAAATATCCTAGACGCTATCGGTTTGGGGGTGGATATGTTTGATTGCGT
GATGCCCACCAGAAACGCCAGAAACGCCACCCTTTTCACGTATTCTGGCAAAATTTCTATCAAAAACGCACCCTATAAAT
TGGATAATACCCCTATTGAAGAAAATTGCGCATGTTATACTTGCAAACGCTATTCTAAAGCCTATTTGCACCATTTGTTT
AGGGCTAAAGAACTCACTTACGCCCGTTTAGCCAGCTTGCACAATTTGCATTTTTATTTAGAGCTGGTTAAAAACGCCAG
AAACGCCATTTTAGAAAAGCGGTTTTTGAGTTTTAAAAAAGAATTTTTGGAGAAATACAACTCCCGCTCTCATTGA

Upstream 100 bases:

>100_bases
TTTTTTGCTACAATGCCATCTAAAATAAGGGCGATTTTTTTCAATTCCAAACTTCCTAAAAAATGATTACGCCATTATAT
CCAAGATTAAGGCTTAAACG

Downstream 100 bases:

>100_bases
ATGATGGAAAGCAAAAATACTAAAAAGCGTTTTTCACCATCAATAAAAGTTTTCTTCAAATAAGGCTTTAGTTTTTAATT
TTTCATTAAAACAAAACCCT

Product: queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase [H]

Number of amino acids: Translated: 371; Mature: 371

Protein sequence:

>371_residues
MDFQLQAVDDNARAGLLNLAHSQVATPVFMPVGTQGCIKSLDATDAQEILGAKLILANTYHMYLRPGEKVVGQLGGLHRF
AQFQGSFLTDSGGFQAFSLSGNIKLQEDGIVFKSHIDGSKHLFTPAKVLDIQYSLNSDIMMVLDDLVGLPAPLKRLEESI
KRSAKWANLSLEYHKQKNRPSNNLFAIIQGGTHLKMRSLSVGLTHEGFDGYAIGGLAVGESVDEMLETIAHTAPLLPKDK
PRYLMGVGTPENILDAIGLGVDMFDCVMPTRNARNATLFTYSGKISIKNAPYKLDNTPIEENCACYTCKRYSKAYLHHLF
RAKELTYARLASLHNLHFYLELVKNARNAILEKRFLSFKKEFLEKYNSRSH

Sequences:

>Translated_371_residues
MDFQLQAVDDNARAGLLNLAHSQVATPVFMPVGTQGCIKSLDATDAQEILGAKLILANTYHMYLRPGEKVVGQLGGLHRF
AQFQGSFLTDSGGFQAFSLSGNIKLQEDGIVFKSHIDGSKHLFTPAKVLDIQYSLNSDIMMVLDDLVGLPAPLKRLEESI
KRSAKWANLSLEYHKQKNRPSNNLFAIIQGGTHLKMRSLSVGLTHEGFDGYAIGGLAVGESVDEMLETIAHTAPLLPKDK
PRYLMGVGTPENILDAIGLGVDMFDCVMPTRNARNATLFTYSGKISIKNAPYKLDNTPIEENCACYTCKRYSKAYLHHLF
RAKELTYARLASLHNLHFYLELVKNARNAILEKRFLSFKKEFLEKYNSRSH
>Mature_371_residues
MDFQLQAVDDNARAGLLNLAHSQVATPVFMPVGTQGCIKSLDATDAQEILGAKLILANTYHMYLRPGEKVVGQLGGLHRF
AQFQGSFLTDSGGFQAFSLSGNIKLQEDGIVFKSHIDGSKHLFTPAKVLDIQYSLNSDIMMVLDDLVGLPAPLKRLEESI
KRSAKWANLSLEYHKQKNRPSNNLFAIIQGGTHLKMRSLSVGLTHEGFDGYAIGGLAVGESVDEMLETIAHTAPLLPKDK
PRYLMGVGTPENILDAIGLGVDMFDCVMPTRNARNATLFTYSGKISIKNAPYKLDNTPIEENCACYTCKRYSKAYLHHLF
RAKELTYARLASLHNLHFYLELVKNARNAILEKRFLSFKKEFLEKYNSRSH

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI13654276, Length=353, Percent_Identity=41.0764872521246, Blast_Score=290, Evalue=1e-78,
Organism=Escherichia coli, GI1786607, Length=367, Percent_Identity=46.3215258855586, Blast_Score=344, Evalue=6e-96,
Organism=Caenorhabditis elegans, GI17544680, Length=368, Percent_Identity=38.3152173913043, Blast_Score=246, Evalue=1e-65,
Organism=Caenorhabditis elegans, GI25146502, Length=171, Percent_Identity=29.8245614035088, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI19920510, Length=357, Percent_Identity=41.1764705882353, Blast_Score=295, Evalue=3e-80,
Organism=Drosophila melanogaster, GI21355311, Length=251, Percent_Identity=27.4900398406374, Blast_Score=96, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004803
- InterPro:   IPR002616 [H]

Pfam domain/function: PF01702 TGT [H]

EC number: =2.4.2.29 [H]

Molecular weight: Translated: 41298; Mature: 41298

Theoretical pI: Translated: 8.84; Mature: 8.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFQLQAVDDNARAGLLNLAHSQVATPVFMPVGTQGCIKSLDATDAQEILGAKLILANTY
CCCEEEEECCCCCHHHHHHHHHHHCCEEEEECCCHHHHHCCCCCCHHHHHCCEEEEEEEE
HMYLRPGEKVVGQLGGLHRFAQFQGSFLTDSGGFQAFSLSGNIKLQEDGIVFKSHIDGSK
EEEECCCHHHHHHHHHHHHHHHHCCCEEECCCCEEEEEECCCEEEECCCEEEEECCCCCC
HLFTPAKVLDIQYSLNSDIMMVLDDLVGLPAPLKRLEESIKRSAKWANLSLEYHKQKNRP
EEECCCEEEEEEECCCCCHHHHHHHHHCCCHHHHHHHHHHHHCCHHCCCEEEEHHHCCCC
SNNLFAIIQGGTHLKMRSLSVGLTHEGFDGYAIGGLAVGESVDEMLETIAHTAPLLPKDK
CCCEEEEEECCCEEEEEEEEEECEECCCCCEEECCCHHHHHHHHHHHHHHHHCCCCCCCC
PRYLMGVGTPENILDAIGLGVDMFDCVMPTRNARNATLFTYSGKISIKNAPYKLDNTPIE
CCEEEECCCHHHHHHHHHCCHHHHHHHCCCCCCCCEEEEEEECEEEECCCCEECCCCCCC
ENCACYTCKRYSKAYLHHLFRAKELTYARLASLHNLHFYLELVKNARNAILEKRFLSFKK
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EFLEKYNSRSH
HHHHHHCCCCC
>Mature Secondary Structure
MDFQLQAVDDNARAGLLNLAHSQVATPVFMPVGTQGCIKSLDATDAQEILGAKLILANTY
CCCEEEEECCCCCHHHHHHHHHHHCCEEEEECCCHHHHHCCCCCCHHHHHCCEEEEEEEE
HMYLRPGEKVVGQLGGLHRFAQFQGSFLTDSGGFQAFSLSGNIKLQEDGIVFKSHIDGSK
EEEECCCHHHHHHHHHHHHHHHHCCCEEECCCCEEEEEECCCEEEECCCEEEEECCCCCC
HLFTPAKVLDIQYSLNSDIMMVLDDLVGLPAPLKRLEESIKRSAKWANLSLEYHKQKNRP
EEECCCEEEEEEECCCCCHHHHHHHHHCCCHHHHHHHHHHHHCCHHCCCEEEEHHHCCCC
SNNLFAIIQGGTHLKMRSLSVGLTHEGFDGYAIGGLAVGESVDEMLETIAHTAPLLPKDK
CCCEEEEEECCCEEEEEEEEEECEECCCCCEEECCCHHHHHHHHHHHHHHHHCCCCCCCC
PRYLMGVGTPENILDAIGLGVDMFDCVMPTRNARNATLFTYSGKISIKNAPYKLDNTPIE
CCEEEECCCHHHHHHHHHCCHHHHHHHCCCCCCCCEEEEEEECEEEECCCCEECCCCCCC
ENCACYTCKRYSKAYLHHLFRAKELTYARLASLHNLHFYLELVKNARNAILEKRFLSFKK
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EFLEKYNSRSH
HHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA