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The map label for this gene is miaB [H]

Identifier: 108562696

GI number: 108562696

Start: 276148

End: 277461

Strand: Direct

Name: miaB [H]

Synonym: HPAG1_0271

Alternate gene names: 108562696

Gene position: 276148-277461 (Clockwise)

Preceding gene: 108562695

Following gene: 108562697

Centisome position: 17.3

GC content: 42.54

Gene sequence:

>1314_bases
TTGAAAGTTTATATTGAAACCATGGGTTGTGCCATGAATTCTAGGGATAGCGAGCATCTACTAAGCGAACTATCCAAACT
AGACTATAAAGAGACCAATGACCCTAAAATAGCGGATTTGATTTTAATCAACACTTGCAGCGTGCGCGAAAAACCTGAAA
GGAAATTGTTTTCAGAAATCGGGCAATTCGCTAAAATCAAAAAACCCAACGCCAAAATCGGGGTTTGCGGGTGCACTGCA
AGCCACATGGGAGCGGATATTTTGAAAAAAGCCCCGAGCGTGAGCTTTGTGTTAGGGGCTAGGAATGTGTCTAAAATCTC
TCAAGTGATCCATAAAGAAAAAGCGGTTGAAGTGGCGATTGATTATGATGAAAGTGCGTATGCGTTTGAATTTTTTGAAA
AAAAGGCTCAAATCCGATCGTTACTAAACATCTCTATAGGTTGCGATAAAAAATGCGCTTATTGCATTGTCCCGCACACT
AGGGGGAAAGAAATTTCTATCCCTATGGATTTGATTTTAAAAGAAGCTGAGAAATTAGCGAATAACGGCACTAAAGAGCT
CATGCTTTTAGGGCAGAATGTGAATAATTACGGCGTGCGTTTCAGCAGCGAGCATGCGAAAGTGGATTTTAGCGATTTGT
TGGATAAATTGAGCGAAATTCAAGGCATTGAGAGGATACGATTCACTTCGCCTCACCCCTTGCACATGAATGATGGATTT
TTAGAGCGTTTTGCCAAAAACCCTAAAGTGTGCAAGAGTATCCACATGCCCTTACAGAGCGGATCTAGCGCGGTGTTAAA
GATGATGCGAAGGGGTTATAGTAAGGAGTGGTTTTTAAATCGGGTGGAGAGGTTAAAGGCTTTAGTGCCTGAAGTGGGCA
TCAGCACGGATATTATCGTGGGCTTTCCTAATGAGAGCGATAAGGATTTTGAAGACACGATGGAGGTGCTAGAAAAAGTG
CGCTTTGACACGCTCTATAGTTTCATTTACTCCCCACGCCCTTTCACTGAAGCGGGAGCTTGGAAAGAAAGAGTGCCATT
AGAGGTTTCATATTCAAGGTTGGAGAGGTTGCAAAACAGGCACAAAGAAATTTTAGAGGAAAAAGCCAAGTTGGAAGTGG
GTAAAACGCATGTGGTGTTGGTGGAAAATAGGCGTGAAATGGATAATCAAATCGTGGGTTTTGAAGGGCGCAGCGATACG
GGGAAATTCATTGAAGTAACTTGTAAGGAAAAAAGAAACCCGGGCGAGCTTGTAAAAGTGGAGATTATTTCTCATTCCAA
AGGGCGCTTGATAGCGGCCATTAAAGGCAACTAA

Upstream 100 bases:

>100_bases
TTTCAAAGAAAAAGGCCATACGGCTTATTTAGATGAGGTGAGGGTCAGCACTGATGAAAAAGATTTTCTTTATGAATTGC
ACATTATTTAAAGGCTTGTA

Downstream 100 bases:

>100_bases
TCAAAATAACCAATGAAGAGTGGGTTTAAAGGCGAGAATTGAGCTTAAAATTTTTCAGGAAAAATATCGTTTTAAAGGTT
GTCCCTCGTTTGGCGTTTGG

Product: (dimethylallyl)adenosine tRNA methylthiotransferase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase [H]

Number of amino acids: Translated: 437; Mature: 437

Protein sequence:

>437_residues
MKVYIETMGCAMNSRDSEHLLSELSKLDYKETNDPKIADLILINTCSVREKPERKLFSEIGQFAKIKKPNAKIGVCGCTA
SHMGADILKKAPSVSFVLGARNVSKISQVIHKEKAVEVAIDYDESAYAFEFFEKKAQIRSLLNISIGCDKKCAYCIVPHT
RGKEISIPMDLILKEAEKLANNGTKELMLLGQNVNNYGVRFSSEHAKVDFSDLLDKLSEIQGIERIRFTSPHPLHMNDGF
LERFAKNPKVCKSIHMPLQSGSSAVLKMMRRGYSKEWFLNRVERLKALVPEVGISTDIIVGFPNESDKDFEDTMEVLEKV
RFDTLYSFIYSPRPFTEAGAWKERVPLEVSYSRLERLQNRHKEILEEKAKLEVGKTHVVLVENRREMDNQIVGFEGRSDT
GKFIEVTCKEKRNPGELVKVEIISHSKGRLIAAIKGN

Sequences:

>Translated_437_residues
MKVYIETMGCAMNSRDSEHLLSELSKLDYKETNDPKIADLILINTCSVREKPERKLFSEIGQFAKIKKPNAKIGVCGCTA
SHMGADILKKAPSVSFVLGARNVSKISQVIHKEKAVEVAIDYDESAYAFEFFEKKAQIRSLLNISIGCDKKCAYCIVPHT
RGKEISIPMDLILKEAEKLANNGTKELMLLGQNVNNYGVRFSSEHAKVDFSDLLDKLSEIQGIERIRFTSPHPLHMNDGF
LERFAKNPKVCKSIHMPLQSGSSAVLKMMRRGYSKEWFLNRVERLKALVPEVGISTDIIVGFPNESDKDFEDTMEVLEKV
RFDTLYSFIYSPRPFTEAGAWKERVPLEVSYSRLERLQNRHKEILEEKAKLEVGKTHVVLVENRREMDNQIVGFEGRSDT
GKFIEVTCKEKRNPGELVKVEIISHSKGRLIAAIKGN
>Mature_437_residues
MKVYIETMGCAMNSRDSEHLLSELSKLDYKETNDPKIADLILINTCSVREKPERKLFSEIGQFAKIKKPNAKIGVCGCTA
SHMGADILKKAPSVSFVLGARNVSKISQVIHKEKAVEVAIDYDESAYAFEFFEKKAQIRSLLNISIGCDKKCAYCIVPHT
RGKEISIPMDLILKEAEKLANNGTKELMLLGQNVNNYGVRFSSEHAKVDFSDLLDKLSEIQGIERIRFTSPHPLHMNDGF
LERFAKNPKVCKSIHMPLQSGSSAVLKMMRRGYSKEWFLNRVERLKALVPEVGISTDIIVGFPNESDKDFEDTMEVLEKV
RFDTLYSFIYSPRPFTEAGAWKERVPLEVSYSRLERLQNRHKEILEEKAKLEVGKTHVVLVENRREMDNQIVGFEGRSDT
GKFIEVTCKEKRNPGELVKVEIISHSKGRLIAAIKGN

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine [H]

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI28872784, Length=455, Percent_Identity=35.1648351648352, Blast_Score=246, Evalue=4e-65,
Organism=Homo sapiens, GI28872782, Length=455, Percent_Identity=35.3846153846154, Blast_Score=245, Evalue=5e-65,
Organism=Homo sapiens, GI93277076, Length=368, Percent_Identity=28.804347826087, Blast_Score=155, Evalue=7e-38,
Organism=Escherichia coli, GI1786882, Length=425, Percent_Identity=36.7058823529412, Blast_Score=269, Evalue=2e-73,
Organism=Escherichia coli, GI1787057, Length=447, Percent_Identity=27.0693512304251, Blast_Score=167, Evalue=2e-42,
Organism=Caenorhabditis elegans, GI17553146, Length=466, Percent_Identity=28.755364806867, Blast_Score=203, Evalue=2e-52,
Organism=Caenorhabditis elegans, GI71996771, Length=257, Percent_Identity=31.5175097276265, Blast_Score=112, Evalue=5e-25,
Organism=Drosophila melanogaster, GI21356207, Length=431, Percent_Identity=31.322505800464, Blast_Score=231, Evalue=5e-61,
Organism=Drosophila melanogaster, GI19922432, Length=456, Percent_Identity=27.1929824561404, Blast_Score=151, Evalue=7e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792 [H]

Pfam domain/function: PF04055 Radical_SAM; PF00919 UPF0004 [H]

EC number: NA

Molecular weight: Translated: 49534; Mature: 49534

Theoretical pI: Translated: 8.74; Mature: 8.74

Prosite motif: PS50926 TRAM ; PS01278 UPF0004

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVYIETMGCAMNSRDSEHLLSELSKLDYKETNDPKIADLILINTCSVREKPERKLFSEI
CEEEEEECCCCCCCCCHHHHHHHHHHCCCCCCCCCCEEEEEEEECCCCCCCHHHHHHHHH
GQFAKIKKPNAKIGVCGCTASHMGADILKKAPSVSFVLGARNVSKISQVIHKEKAVEVAI
HHHHHCCCCCCCEEEEECCHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHCCEEEEE
DYDESAYAFEFFEKKAQIRSLLNISIGCDKKCAYCIVPHTRGKEISIPMDLILKEAEKLA
ECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCEECCCHHHHHHHHHHHH
NNGTKELMLLGQNVNNYGVRFSSEHAKVDFSDLLDKLSEIQGIERIRFTSPHPLHMNDGF
CCCCEEEEEEECCCCCCCCEECCCCCCCCHHHHHHHHHHHCCHHHEECCCCCCCCCCCHH
LERFAKNPKVCKSIHMPLQSGSSAVLKMMRRGYSKEWFLNRVERLKALVPEVGISTDIIV
HHHHHCCHHHHHHHHCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCEEEE
GFPNESDKDFEDTMEVLEKVRFDTLYSFIYSPRPFTEAGAWKERVPLEVSYSRLERLQNR
ECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHCCCCEECHHHHHHHHHH
HKEILEEKAKLEVGKTHVVLVENRREMDNQIVGFEGRSDTGKFIEVTCKEKRNPGELVKV
HHHHHHHHHHHCCCCEEEEEECCCCCCCCCEECCCCCCCCCCEEEEEECCCCCCCCEEEE
EIISHSKGRLIAAIKGN
EEEECCCCCEEEEEECC
>Mature Secondary Structure
MKVYIETMGCAMNSRDSEHLLSELSKLDYKETNDPKIADLILINTCSVREKPERKLFSEI
CEEEEEECCCCCCCCCHHHHHHHHHHCCCCCCCCCCEEEEEEEECCCCCCCHHHHHHHHH
GQFAKIKKPNAKIGVCGCTASHMGADILKKAPSVSFVLGARNVSKISQVIHKEKAVEVAI
HHHHHCCCCCCCEEEEECCHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHCCEEEEE
DYDESAYAFEFFEKKAQIRSLLNISIGCDKKCAYCIVPHTRGKEISIPMDLILKEAEKLA
ECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCEECCCHHHHHHHHHHHH
NNGTKELMLLGQNVNNYGVRFSSEHAKVDFSDLLDKLSEIQGIERIRFTSPHPLHMNDGF
CCCCEEEEEEECCCCCCCCEECCCCCCCCHHHHHHHHHHHCCHHHEECCCCCCCCCCCHH
LERFAKNPKVCKSIHMPLQSGSSAVLKMMRRGYSKEWFLNRVERLKALVPEVGISTDIIV
HHHHHCCHHHHHHHHCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCEEEE
GFPNESDKDFEDTMEVLEKVRFDTLYSFIYSPRPFTEAGAWKERVPLEVSYSRLERLQNR
ECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHCCCCEECHHHHHHHHHH
HKEILEEKAKLEVGKTHVVLVENRREMDNQIVGFEGRSDTGKFIEVTCKEKRNPGELVKV
HHHHHHHHHHHCCCCEEEEEECCCCCCCCCEECCCCCCCCCCEEEEEECCCCCCCCEEEE
EIISHSKGRLIAAIKGN
EEEECCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA