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The map label for this gene is grpE [H]

Identifier: 108562535

GI number: 108562535

Start: 115769

End: 116344

Strand: Reverse

Name: grpE [H]

Synonym: HPAG1_0110

Alternate gene names: 108562535

Gene position: 116344-115769 (Counterclockwise)

Preceding gene: 108562536

Following gene: 108562534

Centisome position: 7.29

GC content: 41.15

Gene sequence:

>576_bases
ATGAAAGATGAACACAACCAAGAACACGATCATTTAAGCCAAAAAGAGCCAGAGTCTTATCAAAAGGCTTGCGCTTGCAA
AGAACAACAGGGTGAAGAAAAGCAAGAAGCGAGCGAAAAAGAATGCGAGATTAAGGAAGATTTTGAGCTTAAGTATAAAG
AAATGCACGAAAAATACTTAAGAGTGCATGCGGATTTTGAAAATGTGAAAAAGCGCTTAGAAAGAGACAAGAGCATGGCG
TTAGAGTATGCGTATGAAAAGATCGCATTGGATCTATTGCCGGTGATTGATGCGCTTCTTGGGGCTTACAAGAGCGCTGC
AGAAGAAAATAAAGAGAGCGCTTTAACCAAAGGCTTGGAGCTTACGATGGAAAAGTTGCATGAAGTTTTGGCAAGGCATG
GCATTGAGGGGATTGAATGCTTAGAAGAATTTGATCCTAATTTCCACAATGCGATCATGCAAGTCAAAAGCGAGGAAAAA
GAAAACGGGAAAATCGTGCAGGTTTTGCAACAGGGTTACAAGTATAAGGGTAGGGTTTTGAGGCCGGCAATGGTGAGCAT
TGCTAAAAACGATTAA

Upstream 100 bases:

>100_bases
GCCCCATAGAATTTCAAAACAAGCGGATACAACTGCTTTGCGTGGGGAAATTAGAATGCGATTATGAAGGGTTTTTTCAA
ACGATTTCTGAGGAGGAATA

Downstream 100 bases:

>100_bases
AAACATTTATTTTAAAGGAAAGGATAACGCATGGGAAAAGTTATTGGAATTGATTTAGGGACAACCAACTCCGCAATGGC
GGTTTATGAAGGCAATGAAG

Product: heat shock protein GrpE

Products: NA

Alternate protein names: HSP-70 cofactor [H]

Number of amino acids: Translated: 191; Mature: 191

Protein sequence:

>191_residues
MKDEHNQEHDHLSQKEPESYQKACACKEQQGEEKQEASEKECEIKEDFELKYKEMHEKYLRVHADFENVKKRLERDKSMA
LEYAYEKIALDLLPVIDALLGAYKSAAEENKESALTKGLELTMEKLHEVLARHGIEGIECLEEFDPNFHNAIMQVKSEEK
ENGKIVQVLQQGYKYKGRVLRPAMVSIAKND

Sequences:

>Translated_191_residues
MKDEHNQEHDHLSQKEPESYQKACACKEQQGEEKQEASEKECEIKEDFELKYKEMHEKYLRVHADFENVKKRLERDKSMA
LEYAYEKIALDLLPVIDALLGAYKSAAEENKESALTKGLELTMEKLHEVLARHGIEGIECLEEFDPNFHNAIMQVKSEEK
ENGKIVQVLQQGYKYKGRVLRPAMVSIAKND
>Mature_191_residues
MKDEHNQEHDHLSQKEPESYQKACACKEQQGEEKQEASEKECEIKEDFELKYKEMHEKYLRVHADFENVKKRLERDKSMA
LEYAYEKIALDLLPVIDALLGAYKSAAEENKESALTKGLELTMEKLHEVLARHGIEGIECLEEFDPNFHNAIMQVKSEEK
ENGKIVQVLQQGYKYKGRVLRPAMVSIAKND

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family [H]

Homologues:

Organism=Homo sapiens, GI24308295, Length=170, Percent_Identity=32.3529411764706, Blast_Score=72, Evalue=4e-13,
Organism=Escherichia coli, GI1788967, Length=184, Percent_Identity=34.7826086956522, Blast_Score=102, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17552458, Length=152, Percent_Identity=31.5789473684211, Blast_Score=69, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6324806, Length=168, Percent_Identity=35.1190476190476, Blast_Score=87, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24653432, Length=143, Percent_Identity=35.6643356643357, Blast_Score=81, Evalue=5e-16,

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012 [H]

Pfam domain/function: PF01025 GrpE [H]

EC number: NA

Molecular weight: Translated: 22185; Mature: 22185

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: PS01071 GRPE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDEHNQEHDHLSQKEPESYQKACACKEQQGEEKQEASEKECEIKEDFELKYKEMHEKYL
CCCCCCCHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHH
RVHADFENVKKRLERDKSMALEYAYEKIALDLLPVIDALLGAYKSAAEENKESALTKGLE
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LTMEKLHEVLARHGIEGIECLEEFDPNFHNAIMQVKSEEKENGKIVQVLQQGYKYKGRVL
HHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHH
RPAMVSIAKND
HHHHHHHHCCC
>Mature Secondary Structure
MKDEHNQEHDHLSQKEPESYQKACACKEQQGEEKQEASEKECEIKEDFELKYKEMHEKYL
CCCCCCCHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHH
RVHADFENVKKRLERDKSMALEYAYEKIALDLLPVIDALLGAYKSAAEENKESALTKGLE
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LTMEKLHEVLARHGIEGIECLEEFDPNFHNAIMQVKSEEKENGKIVQVLQQGYKYKGRVL
HHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHH
RPAMVSIAKND
HHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA